Effects of intensification of the amazon river prawn, macrobrachium amazonicum, grow-out on effluent quality

Studies to determine suitable levels of intensification are essential for developing sustainable aquaculture. The objective of this study was to evaluate the quality of effluents discharged from ponds stocked with 10 (D10), 20 (D20), 40 (D40), and 80 (D80) postlarvae of Macrobrachium amazonicum/m2. Intake and effluent water samples were taken throughout a 5.5-mo grow-out cycle. In that study, twelve 0.01-ha earthen ponds were stocked postlarvae with 0.01g. Average water exchange rate was 15%/d; water was discharged from the bottom of the ponds. Prawns were fed a commercial feed with 38% crude protein according to their biomass (3-10%) and the concentration of dissolved oxygen (DO). In our research, temperature, turbidity, total suspended solids, conductivity, DO, pH, biochemical oxygen demand (BOD), chemical oxygen demand (COD), N-ammonia, N-nitrite, N-nitrate, N-Kjeldahl nitrogen, total phosphorus, and soluble orthophosphate were measured every 15d throughout the experiment in the early morning (0630 to 0730h). Turbidity was lower in D10 than in D20 and D40 and total phosphorus was higher in D80 than in D10 and D20. An analysis of principal components comparing treatments and intake water showed three groups: intake, D10 and a cluster of D20, D40, and D80. On the basis of the water characteristics found in our study it appears that the farming of M. amazonicum is likely to have a low environmental impact, at least up to a stocking density of 80prawns/m2. © by the World Aquaculture Society 2013.

Subcellular localization and kinetic characterization of a gill (Na +, K+)-ATPase from the giant freshwater prawn macrobrachium rosenbergii

The stimulation by Mg2+, Na+, K+, NH 4 +, and ATP of (Na+, K+)-ATPase activity in a gill microsomal fraction from the freshwater prawn Macrobrachium rosenbergii was examined. Immunofluorescence labeling revealed that the (Na +, K+)-ATPase α-subunit is distributed predominantly within the intralamellar septum, while Western blotting revealed a single α-subunit isoform of about 108 kDa M r. Under saturating Mg2+, Na+, and K+ concentrations, the enzyme hydrolyzed ATP, obeying cooperative kinetics with V M = 115.0 ± 2.3 U mg-1, K 0.5 = 0.10 ± 0.01 mmol L-1. Stimulation by Na+ (V M = 110.0 ± 3.3 U mg-1, K 0.5 = 1.30 ± 0.03 mmol L -1), Mg2+ (V M = 115.0 ± 4.6 U mg -1, K 0.5 = 0.96 ± 0.03 mmol L-1), NH4 + (V M = 141.0 ± 5.6 U mg -1, K 0.5 = 1.90 ± 0.04 mmol L-1), and K+ (V M = 120.0 ± 2.4 U mg-1, K M = 2.74 ± 0.08 mmol L-1) followed single saturation curves and, except for K+, exhibited site-site interaction kinetics. Ouabain inhibited ATPase activity by around 73 % with K I = 12.4 ± 1.3 mol L-1. Complementary inhibition studies suggest the presence of F0F1-, Na+-, or K +-ATPases, but not V(H+)- or Ca2+-ATPases, in the gill microsomal preparation. K+ and NH4 + synergistically stimulated enzyme activity (≈25 %), suggesting that these ions bind to different sites on the molecule. We propose a mechanism for the stimulation by both NH4 +, and K+ of the gill enzyme. © 2013 Springer Science+Business Media New York.

Spatial distribution of the amazon river prawn Macrobrachium Amazonicum (Heller, 1862) (Decapoda, Caridea, Palaemonidae) in two perennial creeks of an estuary on the northern coast of Brazil (Guajará Bay, Belém, Pará)

Macrobrachium amazonicum é o crustáceo dulcícola mais frequentemente consumido pelas populações ribeirinhas da Amazônia. Entretanto, apesar de sua relativa abundância e vasto conhecimento da espécie a partir de dados de cultivo, pouco se conhece sobre sua biologia no ambiente natural, especialmente quanto à forma de utilização de canais fortemente influenciados pelas marés. Amostras desse camarão foram coletadas em setembro/06 (período seco) e março/07 (período chuvoso), em dois canais perenes da Baía do Guajará, Pará, utilizando armadilhas (matapis) como as utilizadas pelos pescadores locais para identificar a distribuição espacial dos organismos. Os canais de maré são utilizados por camarões de todos os tamanhos nas duas estações do ano, inclusive para a reprodução. A maior abundância de indivíduos da espécie foi encontrada no período seco, nas áreas a montante dos canais. Postula-se que a abundante matéria orgânica alóctone e a busca de locais protegidos podem ser os fatores que explicam a concentração da abundância e da atividade reprodutiva nas áreas a montante dos canais.

Reproductive variability of the Amazon River prawn, Macrobrachium amazonicum (Caridea, Palaemonidae): influence of life cycle on egg production

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Assessing the potential of partial replacing of artemia by practical inert diet in the larviculture of the Amazon River prawn

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

DIGESTIVE PROTEASES FROM WILD AND FARMED MALE MORPHOTYPES OF THE AMAZON RIVER PRAWN (MACROBRACHIUM AMAZONICUM)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Effect of tank colour on larval performance of the Amazon River prawn Macrobrachium amazonicum

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Effects of ambient nitrite on amazon river prawn, macrobrachium amazonicum, larvae

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Effect of Water Exchange and Mechanical Aeration on Grow-out of the Amazon River Prawn in Ponds

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Sex and reproductive stage differences in the growth, metabolism, feed, fecal production, excretion and energy budget of the Amazon River prawn (Macrobrachium amazonicum)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Integrated multi-trophic culture of Nile tilapia (Oreochromis niloticus) and Amazon river prawn (Macrobrachium amazonicum) in brackish water

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Comparing environmental impacts of native and introduced freshwater prawn farming in Brazil and the influence of better effluent management using LCA

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Treatment efficiency of effluent prawn culture by wetland with floating aquatic macrophytes arranged in series

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

The ultrastructural evaluation of digestive cells in the hepatopancreas of the Amazon River prawn, Macrobrachium amazonicum

The Amazon River prawn Macrobrachium amazonicum is endemic to tropical South America and is being intensively exploited by artisanal fisheries in Brazil. Limited information is available about the nutritional requirements of M. amazonicum, although the production of this species is now technically feasible. The digestive process in this species is still unclear and investigation into the digestive cells of its hepatopancreatic epithelium is required. Thus, the hepatopancreas from 15 specimens were fixed in Karnovsky Solution and processed for Transmission Electron Microscopy. Our results indicate that E cells are located at the distal portion of the hepatopancreatic tubule and are involved in mitotic activity. The cylindrical R cells are sparse and are only found in the proximal portion of the hepatopancreatic tubule. According to its ultrastructural characteristics, this cell is involved in pinocytosis. M cells are generally found near the R cells. The F cells are scattered throughout the length of the hepatopancreatic tubules, and B cells are observed mainly in the proximal and middle regions. F cells and B cells are likely related to, respectively, the synthesis of enzymes and the intracelular digestion. R- and M cells are probably related to material storage. Thus, these findings provide basic information on the cell types that perform protein digestion in M. amazonicum, and will be useful in further nutritional research. The identification and characterization of digestive cells is an important step towards understanding the digestive mechanisms.