RNA polymerase II/III transcription specificity determined by TATA box orientation.

The TATA box sequence in eukaryotes is located about 25 bp upstream of many genes transcribed by RNA polymerase II (Pol II) and some genes transcribed by RNA polymerase III (Pol III). The TATA box is recognized in a sequence-specific manner by the TATA box-binding protein (TBP), an essential factor involved in the initiation of transcription by all three eukaryotic RNA polymerases. We have investigated the recognition of the TATA box by the Pol II and Pol III basal transcription machinery and its role in establishing the RNA polymerase specificity of the promoter. Artificial templates were constructed that contained a canonical TATA box as the sole promoter element but differed in the orientation of the 8-bp TATA box sequence. As expected, Pol II initiated transcription in unfractionated nuclear extracts downstream of the "forward" TATA box. In distinct contrast, transcription that initiated downstream of the "reverse" TATA box was carried out specifically by Pol III. Importantly, this effect was observed regardless of the source of the DNA either upstream or downstream of the TATA sequence. These findings suggest that TBP may bind in opposite orientations on Pol II and Pol III promoters and that opposite, yet homologous, surfaces of TBP may be utilized by the Pol II and Pol III basal machinery for the initiation of transcription.

Petri Artedi renovati pars I. et II. [III-V] : i.e. bibliotheca et philosophia ichthyologica / cura Iohannis Iulii Walbaumii.

v.1-2

Petri Artedi renovati pars I. et II. [III-V] : i.e. bibliotheca et philosophia ichthyologica / cura Iohannis Iulii Walbaumii.

v.3

National Park Service concessions management : hearings before the Subcommittee on National Parks, Historic Preservation, and Recreation of the Committee on Energy and Natural Resources, United States Senate, One Hundred Seventh Congress, first session to review the National Park Service implementation of management policies and procedures to comply with the provisions of Titles I, II, III, IV, V, VI, VII, and VIII of the National Parks Omnibus Management Act of 1998, March 22, 2001, March 29, 2001.

Shipping list no.: 2001-0261-P.

Voyage autour du monde, fait dans les années MDCCXL, I, II, III, IV /

MTSD 15-1.

Voyage autour du monde, fait dans les années MDCCXL, I, II, III, IV /

MTSD 15.

Tomus II [-III] Rerum Concilii Oecumenici Constantiensis de Pace ac unione Ecclesiae ... /

Mode of access: Internet.

Apéndice a los tomos I,II,III y IV de la obra Decretos del rey D. Fernando VII, o sease Colección de reales resoluciones respectivas a los años de 1814, 815, 816 y 817 /

Indice.

Octauarium Romanum : lectiones II & III Nocturni complectens, recitandas infra Octauas Festorum, praesertim Patronorum locorum, & Titularium Ecclesiarum, quae cum Octauis celebrari debent, iuxta Rubricas Breuiarij Romani : a Sacra Rituum Congregatione ad vsum totius Orbis Ecclesiarum approbatum.

Mode of access: Internet.

La vie de S. Paul, apostre des gentils et docteur de l'eglise : e'claircie par l'ecriture sainte, par l'histoire romaine, & par celle des Juifs : avec des réflexions tirées des SS. Peres ... : tome II [- III]

Mode of access: Internet.

Petri Zornii Bibliotheca antiquaria et exegetica in universam Scripturam S. Vet. et Novi Testamenti ... : Tom. I. Pars I, II, III.

Mode of access: Internet.

Carte varie a supplemento e complemento dei volumi II, III, XI, XII, XIII,XIV, XV, XXII, XXXVI, XLIV, XLV, LXVII, LXVIII della Biblioteca della Società storica subalpina /

Includes "Indice" by Pietro Massia published 1939 as supplement to v. 86, with special t. p.: p. [325]-418.

Indovelse i christendom : Nr. I. II. III. /

Himmelstrup, J. Søren Kierkegaard,

Gramática griega : parte II /

Mode of access: Internet.