729 resultados para Fenótipo morfológico
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The morphologic events related to the prenatal development of the ovaries in 81 Nelore breed embryos and fetuses gathered in a local slaughterhouse, with age range from 26 to 240 days following fecundation were studied. The age of fetuses was estimated from measures taken in the cranium-caudal direction. The sex was identified from macroscopic observations and using Polymerase Chain Reaction (PCR) technique. For histology the gonads were fixed in Bourn's fluid for 24 hours and 5 μm thick section's were stained with hematoxylin-eosin. Formation of gonadal ridge and presence of germinal cells were found within it at 29 and 34 days, respectively. Oogonia and primordial follicles, unlike the growing follicles, exhibited significant differences in diameter in the various periods studied. Positive correlation (P<0.05) was found between the diameter of oogonia and their nucleus as well as between primordial and growing follicles with their oocytes and respective nuclei. The gonad was fully formed at 40 days. Primordial follicles, in the growing stage, and antral follicles first appeared, approximately at 95, 140, and 180 days, respectively. Despite the onset and duration of oogenesis being similar to that of taurine breeds, folliculogenesis initiates at an early stages in the Nelore breed.
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The present study had as its objective the assessment of the possible effects of hydric stress on the growth, physiological characteristics of two different genetic materials from Eucalyptus urograndis. The experiment was carried out in a greenhouse at Faculdade de Ciências Agronômicas of UNESP, campus Botucatu from March to July, 2005. The hydric management was established based on the soil water potential. Two water levels were established, doing the evapotranspired water replacement by pot weighing. Two clones were used, Eucalyptus urograndis 105 and 433, being the first one more resistant to the hydric deficit and the 433 more sensitive to stress. The study was made from a 2×2 factorial (two levels of water × two genetic materials). For the hydric management, the plants were irrigated when they reached a soil water potential of -0.03 MPa or -1.5 MPa. The assessments made were: diffusive water vapor of stomato, transpiration, leaf temperature and leaf water potential. The physiological evaluations throughout the day, in the end of the experiment. Treatments without hydric stress had a higher performance in all studied characteristics, but the clones had no influence. The stomatic resistance followed the potentials, showing higher values in the treatments submitted to hydric deficiency, more intensely for clone 433, being that this also happened with the leaf water potential. The transpiration also followed the leaf water potential and the stomatic resistance more intensely for clone 105 both comparing stressed plants and non-stressed plants. Consequently, the leaf temperatures had higher values for clone 433 on the stressed treatment. Thus, it can be concluded that there was a better performance in plants kept on a soil water potential of -0.03 MPa and a higher resistance to hydric stress for clone 105.
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In the autumn the vas deferens of the Italian variety of domestic quail appeared as a single, thin, and straight duct along its total extension. Thus, transversal histologic sections of this duct showed a circular tubular shape. The pseudoestratified columnar epithelium that lined the vas deferens presented longitudinal folds which invaded the lumen frequently empty of spermatozoa. Although in the winter, spring and summer the usual morphological appearance of the quail's vas deferens was seen as a highly coiling duct. So, each transversal histologic section of the vas deferens, in all the segments, showed parallel cut sections of the duct being irregular in shape and variable in number and interconnected by the adventitial loose connective tissue. In these observations, the tubular lumen was totally performed by spermatozoa and seminal fluid. Consequently, with previous base on the testis kinetics of the Italian quail variety, it was concluded that the spermatozoa production, followed by emission, storage and ejaculation of spermatozoa through the vas deferens did not stop during the winter, spring and summer, but ceased in the autumn the quiescent phase of the circannual reproductive cycle in this bird.
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The objective of this work was to analyze the liver of Leporinus macrocephalus at the macroscopic and histologic level by evaluating the characteristics of the hepatic tissue. The fishes from a fishery in Bauru, S.P., were collected and sacrificed by destroying the spinal cord; afterwards the collected hepatic tissue was fixed and followed by histological routine. Macroscopic analysis of L. macrocephalus liver showed an organ of homogeneous structure, with red-brown color, located in the medial region of the body, caudal to heart and after the gills. It showed 3 pyramidal lobes, one central and two lateral right and left. The histological analysis showed the hepatic tissue constituted by hepatocytes cords surrounded by sinusoids, named muralium duplex. It yet revealed the diffused, distribution bile system by hepatic parenchyma, showing ducts with simple cubic epithelium, surrounded by muscular fibres and connective tissue. Cells of the exocrine pancreatic tissue were observed surrounding blood vessels in acinar arrangement as zymogen granules. Furthermore, there are melanomacrophages centers distributed along the hepatic parenchyma, preferably next to the blood vessels, constituted by cells accumulating material, such as melanin and lipofucsin, whose presence may be related to the nutritional status of the fish.
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The genus Stryphnodendron (S.) belongs to the family Leguminosae, subfamily Mimosoideae, which includes mostly trees of tropical and subtropical South America. Extracts of the stem bark are used traditionally by the local population to treat leucorrhoea and diarrhoea, as anti-inflammatory and antiseptic agents (antimicrobial) and to promote blood clotting and wound healing, and in a few cases of gastric ulcers. A review of the literature presented a previous morpho-anatomical study only for S. adstringens (Mart.) Coville. The aim of the present work is to compare morpho-anatomically the stem bark and leaves of three species of Stryphnodendron, known popularly as barbatimão: S. adstringens, S. polyphyllum and S. obovatum, in order to help botanical identification and contribute to quality control. Macro- and microscopical evaluation of the stem barks revealed no significant differences among the species. Morphological analyses of the leaves revealed differences in size, coloration, and pubescence. The leaves of S. adstringens are the largest, glabrous, and concolor. The leaves of S. polyphyllum are smaller, pubescent, and discolor; whereas the leaves of S. obovatum are the same size as those of S. polyphyllum, however, they are glabrous, and discolor.
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The objective of this study was to analyze the morphological and histological characteristics of eggs from six triatomine species in order to obtain a generic and specific characterization of this group of Trypanosoma cruzi vectors and better understand their phylogenetic and taxonomic aspects. The eggs of the respective species came from the collection of the Laboratório de Triatomíneos e Culicídeos da Faculdade de Saúde Pública/USP, from the Insetário do Serviço Especial de Saúde de Araraquara - SESA and from the Triatomine insectary of the Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos (FIOCRUZ), in Rio de Janeiro. Morphological studies were done with optical microscopy and scanning electron microscopy. Histological analyses used only optical microscopy. All of the species analyzed showed a predominance of hexagonal cells. The exchorion of Triatoma breyeri Del Ponte, T. costalimai Verano & Galvão, and T. tibiamaculata (Pinto) consists of unornamented cells, overlapping or slightly separated, with a smooth, padded appearance. Meanwhile, in T. matogrossensis Leite & Barbosa, T. sherlocki Papa, Juberg, Carcavallo, Cerqueira & Barata, and T. williami Galvão, Souza & Lima, the exchorion cells are ornamented on their entire surface with perforations and fissures. Egg histology showed undulations, pores, and orifices. The study helps to expand the generic and specific knowledge of the Triatominae subfamily. The characteristics identified in the exchorion of the eggshells can help separate close species. These new parameters will back the elaboration of future dichotomous keys, help to determine each vector species' role and expand knowledge of the various species.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Pós-graduação em Ciências da Motricidade - IBRC
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Pós-graduação em Ciências da Motricidade - IBRC
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Pós-graduação em Biologia Animal - IBILCE
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)