Detecção da produção de slime por estafilococos coagulase-negativa isolados de cateter venoso central

Slime production is an important virulence factor of coagulase-negative Staphylococcus spp., allowing them to attach to smooth surfaces of biomaterials, and it has been associated with infections of implanted medical devices. In the present study the production of slime capsules in 27 strains of coagulase-negative Staphylococcus was investigated by culture in Congo Red agar (77.7% positivity), spectrophotometric or microplate method (81.4% positivity) and scanning electron microscopy (88.9% positivity). The resistance of coagulase-negative strains of Staphylococcus to various antimicrobial agents was also determined by agar disk diffusion. The proportion of strains resistant to penicillin G, oxacillin, erythromycin, clindamycin and gentamicin among the slime-producing staphylococci was 88.9%, 70.4%, 81.5%, 66.7% and 59.2%, respectively; all of the coagulase-negative staphylococci were susceptible to vancomycin. The strains isolated from central venous catheters were identified by a conventional method and the API Staph system. The 27 coagulase-negative Staphylococcus strains were identified as: S. saprophyticus (3.7%), S. xylosus (7.4%), S. haemolyticus (14.8%), S. epidermidis (37.0%), S. warneri (14.8%), S. lugdunensis (7.4%), S. hominis (7.4%), S. schleiferi (3.7%) and S. chromogenes (3.7%). It can be concluded that in the most of the coagulase-negative Staphylococcus species there was an association between slime production, the nosocomial origin of the strains and reduced sensitivity to the antibiotics, suggesting a pathogenic potential in the hospital environment.

Produção de pimentão em substratos e fertirrigação com efluente de biodigestor

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Estratégias para produção de mudas de tomateiro utilizando paclobutrazol

The modern tomato production, either for fruit table production or for processing, has required quality seedlings, able to withstand environmental adversities after planting and maintaining the standard genotype productive. The aim of this study was to evaluate the effect of three concentrations of PBZ (0, 50 and 100 mg L-1) and two methods of application (seed treatment and watering seedlings) on seedling emergence and seedling production of tomato growth indeterminate. We adopted a completely randomized in a 2x3 factorial scheme with four replications. We evaluated the seedling emergence, the rate of speed of emergence, seedling height and diameter of hypocotyl and their absolute growth rate (TCA), leaf area, dry weight of shoots, roots and total, and reason leaf area (RAF). Results were subjected to analysis of variance and the mean levels of the factor methods of application compared by Tukey test at 5% and the mean levels of the factor concentrations subjected to regression analysis. The application of paclobutrazol via seedlings watering did not affect seedling emergence, significantly reduced the rate of seedling growth and, consequently, the size of the seedlings without affecting the diameter of hypocotyl, plus allow accumulation of dry roots. A concentration of 50 mg L-1 was shown to be sufficient to obtain seedlings more compact, subject to leaf area, the application via watering, beyond allowing an increase of about 37% in dry weight of roots.

Características agronômicas e bromatolôgicas do milho submetido a adubações com potássio na produção de silagem

The aim was to evaluate levels and forms of application of potassium on the agronomic and alimentary characteristics of corn used for silage. The experiment was carried out at UNIFENAS during the agricultural year of 2008/2009. Fertilization at planting and sowing was performed manually using 120kg ha-1 P2O5 and 30kg ha-1 N, the potassium being applied according to the treatments. The experimental design used was of randomized blocks in a 4 x 2 factorial arrangement with four replications, using four amounts (0; 60; 90 and 120 kg ha-1) and two formsof application of K (100% at planting and 50% at sowing+50% coverage). The interaction between the factors of amount and forms of application of the potassium was not significant (p>0.05) for the variables studied: the main effect of each factor was then investigated. There was no effect (p>0.05) for K levels on the percentage of drymatter (DM) and bedded plants, DM and grain productivity and grain participation in the mass. The highest grain yield was obtained when using K at planting (p<0.05). High doses of potassium did not produce changes in DM, percentage of bedded plants, DM production, grain yield or grain percentage of the plant. The different levels and forms of application of potassium do not alter the alimentary characteristics of corn silage.

Doses e modos de aplicação de fósforo na nutrição e produção do feijoeiro cultivar Pérola

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Desenvolvimento ovariano do Prochilodus lineatus em dois sistemas de produção: tanques rede e viveiros escavados

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Susceptibilidade antifúngica, produção de biofilme e caracterização do gene ALS3 em isolados de Candida albicans e não-albicans do hospital das clínicas, UNESP, Botucatu

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeito da silibinina sobre o metabolismo oxidativo e produção de citocinas por monócitos em gestantes portadores de pré-eclâmpsia

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeito da interleucina-15 sobre a atividade fungicida, metabolismo oxidativo e produção de citocinas por monócitos humanos, infectados in vitro com Paracoccidioides brasiliensis

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Stem cell factor estimula células da musculatura lisa da traqueia a produzir TGF-β, FGF-2 E CCL3/MIP-1α

Pós-graduação em Ciências Fisiológicas - FOA

Efeito da suplementação com L-Arginina sobre a produção de mediadores inflamatórios e fatores regenerativos pelo tecido muscular esquelético decorrente de uma sessão de exercício resistido de alta intensidade

Pós-graduação em Ciências Fisiológicas - FOA

Uma abordagem nebulosa de raciocínio baseado em casos para recomendação de sistemas marítimos de produção de petróleo

Pós-graduação em Ciência da Computação - IBILCE

A produção independente de webséries pela perspectiva multiplataforma da televisão digital e internet

Pós-graduação em Televisão Digital: Informação e Conhecimento - FAAC

Ferramentas matemáticas para modelagem da temperatura com aplicação em graus-dias para otimização da produção agrícola

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Produção e caracterização de micro e nanofibras de Poli(fluoreto de vinilideno) - PVDF obtidos pela técnica de fiação por sopro em solução

Pós-graduação em Ciência dos Materiais - FEIS