Avaliação dos aspectos da dieta alimentar de Pimelodus maculatus em dois importantes tributários do reservatório de Jurumirim, Alto Rio Paranapanema-SP

The species Pimelodus maculatus is one of the most abundant fish in many artificial reservoirs. Shows feeding plasticity and exploits all trophic levels in aquatic ecosystems. This study aimed to analyze and compare aspects of the diet of P. maculatus in two important tributaries (Taquari and Veados rivers) in the upper Paranapanema River system, and also investigate possible differences in the condition factor using its feeding habit as reference. Samplings were carried out every three months during 2011 and 2012 in two tributaries of Jurumirim's dam. After biometric analysis, fish were dissected to remove the stomachs that were fixed in formaldehyde 10% and conserved in alcohol 70%. The stomach content was analyzed under stereomicroscope and the food items were identified until the least possible taxonomic level. The diet was characterized based on the Alimentary Index (IAi). Feeding strategy and aspects of species autoecology were evaluated by the relation between abundance of prey-specific (Pi) and occurrence frequency (Fo) of the prey, and also was calculated the niche width of Levin (B). The condition factor was also established and values obtained in samples of both rivers were compared, correlating them with diet of the species

First report of the characterization of the pathophysiological mechanisms caused by the freshwater catfish Pimelodus maculatus (order: Siluriformes)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Pimelodus maculatus e P. platicirris (Siluriformes: pimelodidae) no alto rio Paraná: caracterização morfológica, dieta e reprodução

Pós-graduação em Aquicultura - FCAV

Influence of cage farming on feeding and reproductive aspects of Pimelodus maculatus Lacépède, 1803 (Siluriformes: Pimelodidae) in the Chavantes reservoir, Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Feeding, reproduction and population structure of Pimelodus maculatus Lacépéde, 1803 (Teleostei, Siluriformes, Pimelodiae) from Paraná basin: a review

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Contribución al cultivo de los peces chilenos "Galaxias maculatus" (Jenyns, 1842) y "Genypterus chilensis" (Guichenot, 1848)

Programa de doctorado: Acuicultura: producción controlada de animales acuáticos.

(Figures 2,3) Lipid composition of liver and muscles of the daubed shanny (Leptoclinus maculatus), Kongsfjorden

A comparative study on the lipid composition of the liver and muscles has been performed in daubed shanny caught in summer (July) in Arctic waters at three different sites (biotopes) along the north-western coast of Spitsbergen. In marine organisms living at high latitudes, lipids play an especially important role, primarily as reserve substances and as a factor influencing adaptation to severe environmental conditions. Since the ecology of daubed shanny is poorly known, the data obtained may be considered novel.

Edad y crecimiento de Genypterus maculatus “congrio” capturado frente a Chimbote a bordo del BIC IMARPE V de abril a diciembre del 2014

La investigación tuvo como objetivo determinar la edad y crecimiento de Genypterus maculatus “congrio negro” capturado frente a Chimbote durante el año 2014. La muestra estuvo constituida por 705 pares de otolitos. Del análisis de microincrementos se comprobó que la periodicidad de formación de los anillos de crecimiento fue semestral. Se elaboró una clave talla-edad, al no encontrar diferencias significativas entre sexos. Asimismo, se obtuvo 4 edades (1, 2, 3 y 4 años), siendo el más representativo la edad de 1 año. Se obtuvo la distribución de frecuencias por edad. La relación entre la longitud total del pez y el radio total del otolito fue lineal y la relación longitud total entre el peso total y la longitud fue potencial con un b= 2.89, presentando un crecimiento alométrico negativo. Se estimó los parámetros de crecimiento de von Bertalanffy mediante el programa Table Curve 2D V5. 01, siendo estos L∞ = 101.74 cm; K = 0.124/año; t0 = -0.1997 años. La ecuación de la curva de crecimiento en longitud fue Lt = 101.74*(1-e(- 0.124*(t-0.1997))).

Hogchocker (Trinectes maculatus)

http://digitalcommons.fiu.edu/fce_lter_photos/1293/thumbnail.jpg

Effect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly)

GOMES, Carlos E. M. et al. Effect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly). Plant Physiology and Biochemistry (Paris), v. 43, n. 12, p. 1095-1102, 2005.ISSN 0981-9428. DOI:10.1016/j.plaphy.2005.11.004.

Effect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly)

GOMES, Carlos E. M. et al. Effect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly). Plant Physiology and Biochemistry (Paris), v. 43, n. 12, p. 1095-1102, 2005.ISSN 0981-9428. DOI:10.1016/j.plaphy.2005.11.004.

Determinação de motivos de ligação à quitina em vicilinas de Canavalia ensiformis e Vigna unguiculata através de métodos in silico e relação com suas toxicidades para o bruquídeo Callosobruchus maculatus (Coleoptera:Bruchidae)

Chitin is an important structural component of the cellular wall of fungi and exoskeleton of many invertebrate plagues, such as insects and nematodes. In digestory systems of insects it forms a named matrix of peritrophic membrane. One of the most studied interaction models protein-carbohydrate is the model that involves chitin-binding proteins. Among the involved characterized domains already in this interaction if they detach the hevein domain (HD), from of Hevea brasiliensis (Rubber tree), the R&R consensus domain (R&R), found in cuticular proteins of insects, and the motif called in this study as conglicinin motif (CD), found in the cristallography structure of the β-conglicinin bounded with GlcNac. These three chitin-binding domains had been used to determine which of them could be involved in silico in the interaction of Canavalia ensiformis and Vigna unguiculata vicilins with chitin, as well as associate these results with the WD50 of these vicilins for Callosobruchus maculatus larvae. The technique of comparative modeling was used for construction of the model 3D of the vicilin of V. unguiculata, that was not found in the data bases. Using the ClustalW program it was gotten localization of these domains in the vicilins primary structure. The domains R&R and CD had been found with bigger homology in the vicilins primary sequences and had been target of interaction studies. Through program GRAMM models of interaction ( dockings ) of the vicilins with GlcNac had been gotten. The results had shown that, through analysis in silico, HD is not part of the vicilins structures, proving the result gotten with the alignment of the primary sequences; the R&R domain, although not to have structural similarity in the vicilins, probably it has a participation in the activity of interaction of these with GlcNac; whereas the CD domain participates directly in the interaction of the vicilins with GlcNac. These results in silico show that the amino acid number, the types and the amount of binding made for the CD motif with GlcNac seem to be directly associates to the deleterious power that these vicilins show for C. maculatus larvae. This can give an initial step in the briefing of as the vicilins interact with alive chitin in and exert its toxic power for insects that possess peritrophic membrane

Vicilina de sementes da leguminosa selvagem Anadenanthera macrocarpa: purificação, caracterização, efeito deletério e mecanismo de ação para Callosobruchus maculatus

Grains and legume seeds are foods that form the basis of the diets of many cultures around the world, winch contritbute to the daily nutrient requirements of humans. Vicilins (7S globulin) are storage proteins found in legume seeds, and may have an additional function constitutive defense of the embryo against pests and pathogens. In this work the vicilin from Anadenanthera macrocarpa - AmV (red-angico), was purified and partially characterized, its effect on development and larval survival and adult emergence of Callosobruchus maculatus was evaluated by determination of LD50, WD50 and ED50 in system bioassay. Purification of vicilin was initiated by the chitin affinity chromatography and then gel filtration (Superdex 75 Tricorn 10x300 mm) FPLC system followed by reverse phase chromatography (C8 phenomenex) on HPLC system. Bioassays WD50 and LD50 for larvae were 0.32% and 0.33% (w:w) respectively, since the ED50 for adults was 0.096%. The probable mechanism of action was evaluated by testing digestibility of AmV in vitro, and observed for the involvement of two fragments vicilins immunoreactive against polyclonal Anti-vicilin from Erythrina velutina (Anti-EvV) about of 22 and 13 kDa chitin binding. The AmV in its native form has been recognized by the anti-EvV, indicating that there is a conserved region in the vicilin and is probably corresponding to the chitin binding domains. These results point to a new vicilin chitin binding that can subsequently be used as a possible biopesticide protein source, in order to control insect pest C. maculatus and confirm literature findings that demonstrate vicilin in the presence of different kinds of ligands to conserved regions chitin not yet characterized