997 resultados para Employé
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The vacuolar protein aminopeptidase I (API) uses a novel cytoplasm-to-vacuole targeting (Cvt) pathway. Complementation analysis of yeast mutants defective for cytoplasm-to-vacuole protein targeting (cvt) and autophagy (apg) revealed seven overlapping complementation groups between these two sets of mutants. In addition, all 14 apg complementation groups are defective in the delivery of API to the vacuole. Similarly, the majority of nonoverlapping cvt complementation groups appear to be at least partially defective in autophagy. Kinetic analyses of protein delivery rates indicate that autophagic protein uptake is induced by nitrogen starvation, whereas Cvt is a constitutive biosynthetic pathway. However, the machinery governing Cvt is affected by nitrogen starvation as targeting defects resulting from API overexpression can be rescued by induction of autophagy.
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Sign.: []8, A-2G8/4, H4.
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Mode of access: Internet.
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MTSD 0360.
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MTSD 0360.
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Mode of access: Internet.
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On verso of t.-p.: Copyrighted by the author, Chas. W. Caryl.
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Includes bibliographical references.
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Mode of access: Internet.
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Includes indexes.
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Cover title.
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"GAO-01-368."