Influência dos corantes Azul de Metileno a 2% e Rodamina B a 2% na determinação da capacidade seladora apical de diferentes cimentos endodônticos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Avaliação da infiltração bacteriana corono-apical de canais radiculares obturados com diferentes tipos de cimentos endodônticos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação de solubilidade e desintegração, alteração dimensional e resistência à compressão de cimentos endodônticos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Adesividade de cimentos endodônticos às paredes do canal radicular

Pós-graduação em Odontologia - FOAR

Infiltração bacteriana em obturações realizadas com diferentes cimentos endodônticos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Citotoxicidade, genotoxicidade e expressão gênica em células da polpa dental humana sensibilizadas por cimentos endodônticos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação do PH e da densidade óptica do corante azul de metileno tamponado e não tamponado após o contato com diferentes cimentos endodônticos

O objetivo deste trabalho foi avaliar o pH e a densidade óptica das soluções de azul de metileno a 1% e 2% (tamponadas e não tamponadas) após a imersão de três cimentos endodônticos. Foram preparados oitenta espécimes de cada cimento endodôntico (Endofill, AH Plus e Sealapex), os quais foram imersos nas soluções corantes. As soluções foram analisadas antes e após a imersão dos materiais nos períodos de tempo de 0, 24, 48 e 72h. Foram realizadas avaliações do pH utilizando um pHmetro e da densidade óptica utilizando um espectofotômetro ajustado em 596nm. Os dados de pH foram analisados através de estatística descritiva e os dados da densidade óptica foram analisados pela ANOVA e teste de Tukey 5%. Pôde-se verificar que as soluções corantes de azul de metileno tamponadas e não tamponadas apresentaram pequena variação nos valores de pH e densidade óptica antes do contato com os cimentos endodônticos. As soluções corantes não tamponadas apresentaram valores de pH menores que as tamponadas, independentemente do contato com qualquer cimento endodôntico. Os cimentos endodônticos promoveram alterações nos valores de pH das soluções corantes, sendo que as maiores alterações ocorreram nas soluções não tamponadas. Ocorreram alterações nos valores da densidade óptica das soluções corantes tamponadas e não tamponadas nos diferentes períodos de tempo de análise, sendo diferentes para cada cimento endodôntico utilizado

Infiltração coronária microbiana em canais radiculares obturados pela técnica do cone único com diferentes cimentos endodônticos

With the emergence of new filling materials with different properties and behaviors, the approach of endodontic treatment must be readjusted so that the appropriate result can be achieved. New endodontic sealers include methacrylate resin-based, plant resin-based and the evolution of epoxy-based sealers. This study verified the behavior of new materials that presents controversial results in the literature, about coronal bacterial leakage. That for, 56 single-rooted human teeth were prepared in the direction crown-apex and filled with gutta-percha points with taper of 4% using the single cone technique. Roots were divided randomly into 4 groups according to the sealer (Apexit Plus, AH Plus, EndoREZ and Polifil). After filling, the roots were incorporated in a leakage model, which upper chamber contained a suspension of Streptococcus mutans, and lower chamber a broth, leaving 3 mm of root apical portion immersed. Leakage was assessed for turbidity in lower chamber every day for 60 days. Survival analysis was performed using the nonparametric Kaplan- Meier method (p<0,05). All experimental groups presented leakage during the study’s period, however the maximum time achieve was 22 days. The medium time of leakage was: Apexit Plus 6,3 days, AH Plus 6,3 days and Polifil 5,1 days, but in EndoREZ all specimens infiltrated in the first day, presenting shorter capacity of impermeabilization compared to the other groups. Concluding that none of the sealers tested was able to prevent coronal bacterial leakage

Análise comparativa da ação antimicrobiana dos cimentos endodônticos Acroseal, Sealapex e AH plus pós presa em biofilme de enterococcus faecalis

Pós-graduação em Ciência Odontólogica - FOA

CLSM assessment of tubule penetration and bacterial leakage evaluation of two resin-based sealer

Aim: The aims of this study were to assess the penetration of two endodontic sealers (salicylate and epoxy resin-based sealers) into dentinal tubules using CLSM; and to evaluate the bacterial leakage of roots filled with the same sealers associated with gutta-percha. Material and Methods: For sealer penetrability assessment, thirty bovine roots were instrumented and divided into three groups: AHP: EDTA + filling with AH Plus and gutta-percha (n=10), MTAF: EDTA + filling with MTA Fillapex and gutta-percha (n=10), control group: canals were not irrigated with EDTA and were filled with gutta-percha and AH Plus (n=5) or MTA Fillapex (n=5). Rhodamine B was added to the sealers in order to provide adequate fluorescence. The roots were transversely sectioned 3mm from the apex to enable CLSM analysis. Leakage was evaluated for turbidity of the broth in a split chamber model system for 30 days, using Enterococcus faecalis as a microbial marker. Thirty roots were instrumented and divided in four grupos: AHP: filling with AH Plus and gutta-percha (n=10); MTAF: filling with MTA Fillapex and gutta-percha (n=10); positive control: filling with gutta-percha without sealer (n = 5); negative control: sealing with cyanoacrylate to test the seal of the system (n = 5). Results: The medians for dentinal tubule penetration were 6.8% (AHP) and 6.6% (MTAF) (P = 0.82). The average time for bacterial leakage was 8 days in both experimental groups (P = 0.79). Conclusion: MTA Fillapex and AH Plus presented similar behavior regarding dentinal tubule penetration and bacterial leakage.

Evaluación del comportamiento biológico de tres cementos obturadores endodónticos en tejido subcutáneo de ratones

The aim of this study was to evaluate the biological behavior of the root canal filling sealers: Endofill, Endomethasone and Sealer 26 when in contact with the subcutaneous connective tissue of rat. For the study one control and three experimental groups were used. A total of 15 animals were divided into 5 for each period: 7, 21, and 60 days. The obtained histological sections were processed and stained using the hematoxiline & eosine technique. The histological sections were subjective and comparatively analyzed using optic microscopy. The intensity of the inflammatory reaction and the level of fibrosis of the tissue were registered. The results were registered in scores and statistical analysis by KRUSKAL-WALLIS p<0,05 and MILLER methods. The statistical analysis revealed that in the period of 60 days, there was statistical significance to group II (Endofill) between group (control) and III (Endomethasone) with mononuclear cells into connective tissue. All materials promoted inflammatory reaction more intense at 7 and 21 days with the Endomethasone showing the best results.

The effect of final irrigation on the penetrability of an epoxy resin-based sealer into dentinal tubules: a confocal microscopy study

Introduction The aim of this study was to compare the effect of QMix, BioPure MTAD, 17 % EDTA, and saline on the penetrability of a resin-based sealer into dentinal tubules using a confocal laser scanning microscope (CLSM) and to describe the cleaning of root canal walls by SEM. Methods Eighty distobuccal roots from upper molars were selected and randomly divided into four groups (n=20) before root canal preparation according to the solution used in the final rinse protocol (FRP): QG (QMix), MG (BioPure MTAD), EG (17 % EDTA), and CG (control group: saline). Ten roots of each group were prepared for SEM, and images (×2000) from the canal walls were acquired. The remaining canals were filled with a single gutta-percha cone and AH Plus with 0.1 % Rhodamine B. The specimens were horizontally sectioned at 4 mm from the apex, and the slices were analyzed in CLSM (×10). Sealer penetration was analyzed with Adobe Photoshop software. Results QG and EG presented similar amounts of sealer penetration (P>.05). MG and CG presented the lowest penetrability values (P<.05). The best results for smear layer removal of the apical third of the root canal were achieved by the QG and EG groups when compared with MG and CG (P<.05). Conclusions Seventeen percent EDTA and QMix promoted sealer penetration superior to that achieved by BioPure MTAD and saline. Clinical relevance Despite studies have not confirmed the relationship between sealing ability of endodontic sealers and their penetration in dentinal tubules, sealer penetration assumes importance, since endodontic sealers, unlike guttapercha, are able to penetrate in dentinal tubules, isthmus, and accessory canals, filling the root canal system.

Evaluation of the pH, calcium release and antibacterial activity of MTA Fillapex

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Avaliação da resposta tecidual e capacidade de mineralização de cimentos que contém compostos biocerâmicos, resinosos e com hidróxido de cálcio

Pós-graduação em Ciência Odontólogica - FOA

Propriedades físicas, químicas, antimicrobianas e biológicas de cimentos endodônticos com óxido de cálcio e resina salicilato

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)