State of play: review of environmental policy integration literature. Research series paper no. 7

A report for the National Economic and Social Council (NESC), prepared by the Cleaner Production Promotion Unit, UCC offering an up-to-date review of the Environmental Policy Integration literature in both academic and policy debates. The report provides an overview of the theory and conceptual development; methodologies outlined and presents examples of current applications in policy across Europe and internationally.

Milestones in Software Engineering and Knowledge Engineering History: A Comparative Review

We present a review of the historical evolution of software engineering, intertwining it with the history of knowledge engineering because “those who cannot remember the past are condemned to repeat it.” This retrospective represents a further step forward to understanding the current state of both types of engineerings; history has also positive experiences; some of them we would like to remember and to repeat. Two types of engineerings had parallel and divergent evolutions but following a similar pattern. We also define a set of milestones that represent a convergence or divergence of the software development methodologies. These milestones do not appear at the same time in software engineering and knowledge engineering, so lessons learned in one discipline can help in the evolution of the other one.

Metodología ágil para equipos pequeños usando plataformas Microsoft

Con el objetivo de mejorar el rendimiento de los equipos de desarrollo de software se han definido muchas metodologías de desarrollo, entre las que se encuentran las prescriptivas y las ágiles. Pese a esto, los equipos no suelen emplear ninguna por no encontrarlas ajustadas a su contexto particular, y se van directamente a la tarea de programar. En este artículo se propone una metodología de desarrollo de software ligera y adaptada a un contexto preciso y bien definido, que alinea Microsoft Solutions Framework for Agile Software Development (MSF4ASD) con los lineamientos de gestión de proyectos presentados en la Guía del PMBOK. Además, se brindan elementos para la implementación de una plataforma Microsoft de desarrollo de software en equipo para soportarla.

Business intelligence em sistemas de apoio à gestão de frotas: Análise de Tecnologias e metodologias

O objecto de estudo desta tese de mestrado surgiu da necessidade de dar resposta a uma proposta para uma solução de business intelligence a pedido de um cliente da empresa onde até à data me encontro a desempenhar funções de analista programador júnior. O projecto consistiu na realização de um sistema de monitorização de eventos e análise de operações, portanto um sistema integrado de gestão de frotas com módulo de business intelligence. Durante o decurso deste projecto foi necessário analisar metodologias de desenvolvimento, aprender novas linguagens, ferramentas, como C#, JasperReport, visual studio, Microsoft SQL Server entre outros. ABSTRACT: Business Intelligence applied to fleet management systems - Technologies and Methodologies Analysis. The object of study of this master's thesis was the necessity of responding to a proposal for a business intelligence solution at the request of a client company where so far I find the duties of junior programmer. The project consisted of a system event monitoring and analysis of operations, so an integrated fleet management with integrated business intelligence. During the course of this project was necessary to analyze development methodologies, learn new languages, tools such as C #, JasperReports, visual studio, Microsoft Sql Server and others.

Enhancing emancipatory systems methodologies for sustainable development

Our interest lies in applying the principles of critical systems thinking to human activity systems in developing countries in situations where issues of natural resource sustainability constrain the feasible set of long-term strategies. The concept of sustainable development provides an expanded domain for critical systems thinking. The fundamental values underpinning sustainable development are that both intragenerational and intergenerational equity are important. As a consequence, key stakeholders are often excluded from power-sharing within current social systems. Addressing these issues requires renewed focus on emancipatory commitment and methodologies. To date, Ulrich's critical systems heuristics is the only critical systems methodology that offers practicable tools for emancipation. A case study analysis in Tigray, northern Ethiopia, provides insights in relation to the application of critical system heuristics to issues of sustainable development and highlights the need to extend the use of critical systems heuristics beyond the design and monitoring of structured interventions.

Development of new methodologies in sample treatment for proteomics workflow based on enzymatic probe sonication technology and mass spectrometry

Thesis submitted to the Universidade Nova de Lisboa, Faculdade de Ciências e Tecnologia, for the degree of Doctor of Philosophy in Biochemistry

Transparency in project bond ratings: Assessment of rating methodologies and development of a proven rating simulator

A Work Project, presented as part of the requirements for the Award of a Masters Degree in Finance from the NOVA – School of Business and Economics

Desarrollo de metodologias de propagación y cultivo de plantas nativas de Córdoba con potencialornamental. Development of propagation and cultivation methodologies of native plants of Córdoba withornamental potential.

El problema: La flora nativa de Córdoba, actualmente amenazada, es rica en especies con potencial ornamental que todavía no se cultivan. El uso de estas plantas está limitado por la escasez de material biológico, la falta de conocimientos de su propagación y su insuficiente valoración pública. Hipótesis: La propagación y el cultivo de una amplia gama de plantas nativas cordobesas con potencial ornamental son técnicamente factibles y tienen potencial productivo y económico. Objetivo: Promover el uso de especies nativas ornamentales en la provincia de Córdoba. Objetivos específicos: 1. Desarrollar metodologías de propagación y cultivo de especies nativas con potencial ornamental; 2. Transferir estas metodologías a viveros privados y públicos; 3. Difundir los conocimientos obtenidos a instituciones educativas. Métodos: 1- Colección: Se realizarán viajes de campo para obtener semillas o esquejes de al menos 6 especies nativas seleccionadas. Las semillas se limpiarán y se conservarán en frío. 2- Propagación: En la primavera se sembrarán 100 semillas por especie, accesión y tratamiento; se registrará porcentaje y tiempo de germinación. Los plantines se trasplantarán a almácigos, se registrará supervivencia y crecimiento. Para la propagación vegetativa, se trasplantarán esquejes de estolones directamente a macetas. 3- Trasplante: En verano, los plantines se trasplantarán a macetas grandes; se registrará supervivencia y crecimiento durante un año. 4- Documentación: Se elaborarán protocolos de las metodologías adecuadas para la propagación de las especies, usos ornamentales y características relevantes. 5- Transferencia: Los protocolos, muestras de semillas y de plantas, se transferirán a dos viveros que se comprometan a continuar con el cultivo de las especies. 6- Difusión: Se realizarán cursos, talleres, charlas y pasantías para dar a conocer la propagación de plantas nativas en instituciones educativas, desde la primaria hasta la universidad. Resultados y productos esperados: 1- Protocolos de propagación de al menos 6 especies nativas ornamentales y su transferencia a viveros, como base de una actividad productiva novedosa. 2- Un aporte a la conservación ex situ y el uso sostenible de la flora nativa. 3- Una mayor valoración de esta en la comunidad educativa. Importancia: 1- Desarrollo de la producción de plantas nativas con valor ornamental, como una alternativa económica. 2- Conservación ex situ y uso sostenible de la flora nativa. 3- Difusión del conocimiento del valor ornamental de las nativas. 4. Información científica de la biología y ecología de especies nativas. Pertinencia: Productos (ver Resultados). El impacto inmediato esperado es un aumento en la propagación, la producción, la demanda, la comercialización y el uso de plantas ornamentales nativas. Se espera también la generación de nuevos conocimientos y el estímulo de líneas de investigación biológicas y agronómicas.

Development and application of computational methodologies in qualitative modeling

La présente thèse s'intitule "Développent et Application des Méthodologies Computationnelles pour la Modélisation Qualitative". Elle comprend tous les différents projets que j'ai entrepris en tant que doctorante. Plutôt qu'une mise en oeuvre systématique d'un cadre défini a priori, cette thèse devrait être considérée comme une exploration des méthodes qui peuvent nous aider à déduire le plan de processus regulatoires et de signalisation. Cette exploration a été mue par des questions biologiques concrètes, plutôt que par des investigations théoriques. Bien que tous les projets aient inclus des systèmes divergents (réseaux régulateurs de gènes du cycle cellulaire, réseaux de signalisation de cellules pulmonaires) ainsi que des organismes (levure à fission, levure bourgeonnante, rat, humain), nos objectifs étaient complémentaires et cohérents. Le projet principal de la thèse est la modélisation du réseau de l'initiation de septation (SIN) du S.pombe. La cytokinèse dans la levure à fission est contrôlée par le SIN, un réseau signalant de protéines kinases qui utilise le corps à pôle-fuseau comme échafaudage. Afin de décrire le comportement qualitatif du système et prédire des comportements mutants inconnus, nous avons décidé d'adopter l'approche de la modélisation booléenne. Dans cette thèse, nous présentons la construction d'un modèle booléen étendu du SIN, comprenant la plupart des composantes et des régulateurs du SIN en tant que noeuds individuels et testable expérimentalement. Ce modèle utilise des niveaux d'activité du CDK comme noeuds de contrôle pour la simulation d'évènements du SIN à différents stades du cycle cellulaire. Ce modèle a été optimisé en utilisant des expériences d'un seul "knock-out" avec des effets phénotypiques connus comme set d'entraînement. Il a permis de prédire correctement un set d'évaluation de "knock-out" doubles. De plus, le modèle a fait des prédictions in silico qui ont été validées in vivo, permettant d'obtenir de nouvelles idées de la régulation et l'organisation hiérarchique du SIN. Un autre projet concernant le cycle cellulaire qui fait partie de cette thèse a été la construction d'un modèle qualitatif et minimal de la réciprocité des cyclines dans la S.cerevisiae. Les protéines Clb dans la levure bourgeonnante présentent une activation et une dégradation caractéristique et séquentielle durant le cycle cellulaire, qu'on appelle communément les vagues des Clbs. Cet évènement est coordonné avec la courbe d'activation inverse du Sic1, qui a un rôle inhibitoire dans le système. Pour l'identification des modèles qualitatifs minimaux qui peuvent expliquer ce phénomène, nous avons sélectionné des expériences bien définies et construit tous les modèles minimaux possibles qui, une fois simulés, reproduisent les résultats attendus. Les modèles ont été filtrés en utilisant des simulations ODE qualitatives et standardisées; seules celles qui reproduisaient le phénotype des vagues ont été gardées. L'ensemble des modèles minimaux peut être utilisé pour suggérer des relations regulatoires entre les molécules participant qui peuvent ensuite être testées expérimentalement. Enfin, durant mon doctorat, j'ai participé au SBV Improver Challenge. Le but était de déduire des réseaux spécifiques à des espèces (humain et rat) en utilisant des données de phosphoprotéines, d'expressions des gènes et des cytokines, ainsi qu'un réseau de référence, qui était mis à disposition comme donnée préalable. Notre solution pour ce concours a pris la troisième place. L'approche utilisée est expliquée en détail dans le dernier chapitre de la thèse. -- The present dissertation is entitled "Development and Application of Computational Methodologies in Qualitative Modeling". It encompasses the diverse projects that were undertaken during my time as a PhD student. Instead of a systematic implementation of a framework defined a priori, this thesis should be considered as an exploration of the methods that can help us infer the blueprint of regulatory and signaling processes. This exploration was driven by concrete biological questions, rather than theoretical investigation. Even though the projects involved divergent systems (gene regulatory networks of cell cycle, signaling networks in lung cells), as well as organisms (fission yeast, budding yeast, rat, human), our goals were complementary and coherent. The main project of the thesis is the modeling of the Septation Initiation Network (SIN) in S.pombe. Cytokinesis in fission yeast is controlled by the SIN, a protein kinase signaling network that uses the spindle pole body as scaffold. In order to describe the qualitative behavior of the system and predict unknown mutant behaviors we decided to adopt a Boolean modeling approach. In this thesis, we report the construction of an extended, Boolean model of the SIN, comprising most SIN components and regulators as individual, experimentally testable nodes. The model uses CDK activity levels as control nodes for the simulation of SIN related events in different stages of the cell cycle. The model was optimized using single knock-out experiments of known phenotypic effect as a training set, and was able to correctly predict a double knock-out test set. Moreover, the model has made in silico predictions that have been validated in vivo, providing new insights into the regulation and hierarchical organization of the SIN. Another cell cycle related project that is part of this thesis was to create a qualitative, minimal model of cyclin interplay in S.cerevisiae. CLB proteins in budding yeast present a characteristic, sequential activation and decay during the cell cycle, commonly referred to as Clb waves. This event is coordinated with the inverse activation curve of Sic1, which has an inhibitory role in the system. To generate minimal qualitative models that can explain this phenomenon, we selected well-defined experiments and constructed all possible minimal models that, when simulated, reproduce the expected results. The models were filtered using standardized qualitative ODE simulations; only the ones reproducing the wave-like phenotype were kept. The set of minimal models can be used to suggest regulatory relations among the participating molecules, which will subsequently be tested experimentally. Finally, during my PhD I participated in the SBV Improver Challenge. The goal was to infer species-specific (human and rat) networks, using phosphoprotein, gene expression and cytokine data and a reference network provided as prior knowledge. Our solution to the challenge was selected as in the final chapter of the thesis.

Development of new methodologies for entry to targets of therapeutic interest

This Account provides an overview of strategies that have been reported from our laboratories for the synthesis of targets of therapeutic interest, namely carbohydrates, and prodrugs for the treatment of melanoma. These programmes have involved the development of new synthetic methodologies including the regio- and stereoselective synthesis of specific carbohydrate isomers, and new protecting group methodologies. This review provides an insight into the progress of these research themes, and suggests some applications for the targets that are currently being explored.

New technologies and methodologies for intervention to promote development

Includes bibliography

Design and development of new green catalytic methodologies for the synthesis of enantiomerically enriched molecules

The following Ph.D work was mainly focused on catalysis, as a key technology, to achieve the objectives of sustainable (green) chemistry. After introducing the concepts of sustainable (green) chemistry and an assessment of new sustainable chemical technologies, the relationship between catalysis and sustainable (green) chemistry was briefly discussed and illustrated via an analysis of some selected and relevant examples. Afterwards, as a continuation of the ongoing interest in Dr. Marco Bandini’s group on organometallic and organocatalytic processes, I addressed my efforts to the design and development of novel catalytic green methodologies for the synthesis of enantiomerically enriched molecules. In the first two projects the attention was focused on the employment of solid supports to carry out reactions that still remain a prerogative of omogeneous catalysis. Firstly, particular emphasis was addressed to the discovery of catalytic enantioselective variants of nitroaldol condensation (commonly termed Henry reaction), using a complex consisting in a polyethylene supported diamino thiopene (DATx) ligands and copper as active species. In the second project, a new class of electrochemically modified surfaces with DATx palladium complexes was presented. The DATx-graphite system proved to be efficient in promoting the Suzuki reaction. Moreover, in collaboration with Prof. Wolf at the University of British Columbia (Vancouver), cyclic voltammetry studies were reported. This study disclosed new opportunities for carbon–carbon forming processes by using heterogeneous, electrodeposited catalyst films. A straightforward metal-free catalysis allowed the exploration around the world of organocatalysis. In fact, three different and novel methodologies, using Cinchona, Guanidine and Phosphine derivatives, were envisioned in the three following projects. An interesting variant of nitroaldol condensation with simple trifluoromethyl ketones and also their application in a non-conventional activation of indolyl cores by Friedel-Crafts-functionalization, led to two novel synthetic protocols. These approaches allowed the preparation of synthetically useful trifluoromethyl derivatives bearing quaternary stereocenters. Lastly, in the sixth project the first γ-alkylation of allenoates with conjugated carbonyl compounds was envisioned. In the last part of this Ph.D thesis bases on an extra-ordinary collaboration with Prof. Balzani and Prof. Gigli, I was involved in the synthesis and characterization of a new type of heteroleptic cyclometaled-Ir(III) complexes, bearing bis-oxazolines (BOXs) as ancillary ligands. The new heteroleptic complexes were fully characterized and in order to examine the electroluminescent properties of FIrBOX(CH2), an Organic Light Emitting Device was realized.