Levantamento fitossociológico em pastagens degradadas sob condições de várzea

O levantamento foi realizado em duas áreas de várzea, eventualmente inundáveis, localizadas na Fazenda Experimental de Leopoldina, da Empresa de Pesquisa Agropecuária de Minas Gerais. A área 1, de 3 ha, estava ocupada por uma pastagem de capim-angola (Brachiaria mutica), mal manejada e sem controle de plantas daninhas há mais de dez anos. A área 2, de 5 ha, estava ocupada por uma pastagem de capim-setária (Setaria anceps cv. Kazungula), implantada na estação chuvosa do ano anterior, cuja formação ficou prejudicada pelo baixo estabelecimento da forrageira. Para o estudo fitossociológico, utilizou-se o método do quadrado inventário, aplicado por meio de um quadrado de 1,0 m², lançado ao acaso 19 vezes na área 1 e 41 vezes na área 2. As espécies encontradas foram identificadas e cadastradas. Na pastagem de capim-angola foram identificadas 27 espécies, distribuídas em 11 famílias e na pastagem de capim-setária 34 espécies distribuídas em 13 famílias. As famílias mais representativas em número de espécies foram: Poaceae (11), Asteraceae (6), Papilionoideae (5), Malvaceae e Euphorbiaceae (4). As maiores freqüências foram das seguintes espécies: Cynodon dactylon, Sida rhombifolia, Cyperus esculentus, Mimosa pudica, Senna occidentalis, Setaria anceps cv. Kazungula e Paspalum urvillei. Em geral, as duas áreas apresentaram-se infestadas com plantas daninhas, inclusive com plantas tóxicas, espinescentes e de baixa palatabilidade, reduzindo a capacidade de suporte animal dos pastos e impedindo o aproveitamento adequado das áreas pelos bovinos.

Fungal community associated with marine macroalgae from Antarctica

Filamentous fungi and yeasts associated with the marine algae Adenocystis utricularis, Desmarestia anceps, and Palmaria decipiens from Antarctica were studied. A total of 75 fungal isolates, represented by 27 filamentous fungi and 48 yeasts, were isolated from the three algal species and identified by morphological, physiological, and sequence analyses of the internal transcribed spacer region and D1/D2 variable domains of the large-subunit rRNA gene. The filamentous fungi and yeasts obtained were identified as belonging to the genera Geomyces, Antarctomyces, Oidiodendron, Penicillium, Phaeosphaeria, Aureobasidium, Cryptococcus, Leucosporidium, Metschnikowia, and Rhodotorula. The prevalent species were the filamentous fungus Geomyces pannorum and the yeast Metschnikowia australis. Two fungal species isolated in our study, Antarctomyces psychrotrophicus and M. australis, are endemic to Antarctica. This work is the first study of fungi associated with Antarctic marine macroalgae, and contributes to the taxonomy and ecology of the marine fungi living in polar environments. These fungal species may have an important role in the ecosystem and in organic matter recycling.

Hemoparasites of the genus Trypanosoma (Kinetoplastida: Trypanosomatidae) and hemogregarines in Anurans of the São Paulo and Mato Grosso do Sul States - Brazil

Os animais silvestres estão expostos a inúmeros patógenos,dentre eles estão os hemoparasitas. Podem-se destacar espécies do gênero Trypanosoma e do grupo das hemogregarinas,que ocorrem com freqüência parasitando anuros (rãs, pererecas e sapos). Normalmente, a descrição destes hemoparasitas é feita através da morfologia dos estágios observados nosangue periférico do hospedeiro e as pesquisas sobre o ciclobiológico desses hemoparasitas são escassas. Os objetivos dopresente estudo foram avaliar a presença de hemogregarinas eTrypanosoma spp. em anuros capturados nos Estados de São Paulo e Mato Grosso do Sul e fazer a caracterização morfológica e morfométrica dos seus hemoparasitas. As espécies deanuros examinadas foram: Dendropsophus nanus, D. minutus, Leptodactylus chaquensis, L. podicipinus, L. labyrinthicus, L. fuscus, Bufo granulosus, B. schneideri, Phyllomedusahypocondrialis, Trachicephalus venulosus, Scinax fuscovarius e Hypsiboas albopunctatus. Dos 40 animais estudados, foramencontrados quatro (10%) positivos para hemogregarinas e oito(20%) positivos para Trypanosoma spp. Foram observadosgamontes de hemogregarinas com morfologia variável e, alémdas formas intraeritrocíticas, também foram observados gamontes fora das hemácias. As formas de Trypanosoma encontradas eram muito polimórficas, conforme é descrito na literatura, sendo na sua maioria, larga e oval.

Anurofauna em área de cerrado aberto no município de Borebi, estado de São Paulo, Sudeste do Brasil: uso do habitat, abundância e variação sazonal

O bioma Cerrado encontra-se descaracterizado e menos de três por cento de suas áreas originais está legalmente protegida. A anurofauna desse bioma não é muito rica quando comparado a outros biomas, porém há um grande número de espécies endêmicas. Aqui apresentamos uma lista de espécies de anuros registrados em uma lagoa em área de cerrado aberto do município de Borebi, região Centro-Oeste do estado de São Paulo, sudeste do Brasil. Durante 24 meses de estudo (2008 e 2009) caracterizamos a distribuição das espécies na lagoa estudada e descrevemos a variação sazonal das espécies. Foram registradas 27 espécies pertencentes a seis famílias: Bufonidae (duas espécies), Cycloramphidae (uma espécie), Hylidae (13 espécies), Leiuperidae (quatro espécies), Leptodactylidae (cinco espécies) e Microhylidae (duas espécies). A riqueza de espécies e abundância estiveram relacionadas com a precipitação. Dendropsophus minutus foi a espécie mais abundante e com registro de vocalização durante o ano inteiro. Rhinella ornata e Odontophrynus americanus foram restritas ao período seco e frio (abril a agosto). As outras espécies tiveram seu período de maior atividade nos meses chuvosos e quentes (setembro a março). A ocupação da lagoa variou com o tipo de vegetação e conforme a variação do seu volume de água, principalmente nos período de estiagem. A alta riqueza e abundância de anuros da lagoa pode ser resultado da ausência de peixes predadores, dos diversos tipos de microambientes do local e da ausência de outros corpos d'água próximos.

Bioacústica e sítio de vocalização em taxocenoses de anuros de área aberta no noroeste paulista

O sítio de vocalização e os parâmetros acústicos do canto de anúncio foram determinados para 18 espécies de anuros em seis corpos d'água na região noroeste paulista. A freqüência dominante do canto de anúncio foi positivamente correlacionada com o CRC das espécies: as espécies pequenas vocalizaram em uma freqüência mais alta e as maiores vocalizaram em uma freqüência mais baixa. A similaridade nos parâmetros espectrais e temporais do canto de anúncio foi alta entre 94% das espécies registradas. A similaridade no uso do sítio de vocalização foi baixa, apenas 22% das espécies apresentaram alta sobreposição, apesar de cerca de metade das espécies terem sido generalistas no uso de sítio de vocalização. Analisando conjuntamente a similaridade no uso de sítio de vocalização, no hábitat e nos parâmetros do canto de anúncio, a proporção de espécies com alta sobreposição diminui de 94% para 11%, correspondente à sobreposição entre um único par de espécies: Dendropsophus elianeae e D. minutus. Espécies com sobreposição no sítio de vocalização apresentaram segregação nos parâmetros acústicos e, espécies com sobreposição nos parâmetros acústicos tenderam a partilhar sítio de vocalização. Esse resultado sugere a ocorrência de complementaridade de nicho, pois as espécies que ocuparam uma posição similar em uma dimensão tenderam a diferir em outra dimensão. A homogeneidade estrutural dos corpos d'água, a severidade climática da região e o elevado grau de conversão do ambiente natural em áreas de cultivo limitam a abundância das populações e a riqueza de espécies. A baixa sobreposição no sítio de vocalização está, provavelmente, associada a insaturação das comunidades amostradas.

Diversidade e distribuição espaço-temporal de anuros em região com pronunciada estação seca no sudeste do Brasil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A comparative study of the extracutaneous pigmentary system in three anuran amphibian species evaluated during the breeding season.

The present investigation examined the extracutaneous pigmentation pattern of three species of anuran amphibians, Dendropsophus nanus, Physalaemus cuvieri, and Rhinella schneideri, during the course of their breeding seasons. Pigmentation intensity in the different organs was graded on a 4-category scale, in which category 0 refers to organs without pigment and category 3 refers to intensely pigmented organs, with 2 intermediate stages of progressively stronger pigmentation. Rhinella schneideri showed testicular pigmentation, with intra-specific variation (categories 0 and 1). In P. cuvieri the pigmentation in the lungs varied in time: all the animals showed pigmentation (category 1) at the beginning of the breeding season, and as the season progressed the absence of pigments became the most common pattern. In the liver of the first animals collected, the pigment intensity was high (category 2) with many iridophores present, but in the last specimens collected no iridophores were found. The variation in D. nanus occurred in the kidneys, where animals collected at the beginning of the season did not show pigmentation. Renal veins displayed few melanocytes (category 1); animals collected at the end of the season showed more pigmentation in the kidneys (category 2), whereas in the renal veins the intensity remained the same. The changes observed in the extracutaneous pigment system in some organs, during the reproductive period, may be due to physiological alterations or may represent a species-specific characteristic.

Gênese e Classificação da Coquina de Camaquã, Formação Corumbataí (Neopermiano), na Região de Rio Claro, SP

Analysis of the taphonomic signatures of a well preserved, silicifled coquina (Pinzonella neotropica assemblage) from the Camaquã outcrop, upper part of the Corumbataí Formation (Late Permian), in the Rio Claro region, state of Sáo Paulo, allowed interpretation of processes involved in its origin as related to high energy events (storms). The coquina occurs as a lenticular body, 2-11 cm thick and extending laterally for about 120 m. Basal contact of the coquina is sharp and erosive. Its upper contact is sharp. The concentration is dominated by pelecypods including the shallow burrowers (Pinzonella neotropica, Jacquesia brasiliensis), intermediate burrower (Pyramus anceps) and semi-infaunal forms (Naiadopsis lamellosus). All these species are suspension feeders. Besides sand-sized or even smaller shell fragments, there occur disarticulated, complete shells which are commonly abraded but do not show any signs of bioerosion or incrustation. In vertical side view, the shells are mainly convex-up, nested or stacked, while in plan-view they show random orientation. Multiple discontinuous grading is visible. These taphonomic signatures suggest that the origin of the skeletal accumulation is related to high energy events (possibly storm flows) in a proximal environment. The amalgamated nature of the Camaquã coquina records several episodes of erosion and deposition.

Systematic review of the frog family Hylidae, with special reference to Hylinae: Phylogenetic analysis and taxonomic revision

Hylidae is a large family of American, Australopapuan, and temperate Eurasian treefrogs of approximately 870 known species, divided among four subfamilies. Although some groups of Hylidae have been addressed phylogenetically, a comprehensive phylogenetic analysis has never been presented. The first goal of this paper is to review the current state of hylid systematics. We focus on the very large subfamily Hylinae (590 species), evaluate the monophyly of named taxa, and examine the evidential basis of the existing taxonomy. The second objective is to perform a phylogenetic analysis using mostly DNA sequence data in order to (1) test the monophyly of the Hylidae; (2) determine its constituent taxa, with special attention to the genera and species groups which form the subfamily Hylinae, and c) propose a new, monophyletic taxonomy consistent with the hypothesized relationships. We present a phylogenetic analysis of hylid frogs based on 276 terminals, including 228 hylids and 48 outgroup taxa. Included are exemplars of all but 1 of the 41 genera of Hylidae (of all four nominal subfamilies) and 39 of the 41 currently recognized species groups of the species-rich genus Hyla. The included taxa allowed us to test the monophyly of 24 of the 35 nonmonotypic genera and 25 species groups of Hyla. The phylogenetic analysis includes approximately 5100 base pairs from four mitochondrial (12S, tRNA valine, 16S, and cytochrome b) and five nuclear genes (rhodopsin, tyrosinase, RAG-1, seventh in absentia, and 28S), and a small data set from foot musculature. Concurring with previous studies, the present analysis indicates that Hemiphractinae are not related to the other three hylid subfamilies. It is therefore removed from the family and tentatively considered a subfamily of the paraphyletic Leptodactylidae. Hylidae is now restricted to Hylinae, Pelodryadinae, and Phyllomedusinae. Our results support a sister-group relationship between Pelodryadinae and Phyllomedusinae, which together form the sister taxon of Hylinae. Agalychnis, Phyllomedusa, Litoria, Hyla, Osteocephalus, Phrynohyas, Ptychohyla, Scinax, Smilisca, and Trachycephalus are not monophyletic. Within Hyla, the H. albomarginata, H. albopunctata, H. arborea, H. boons, H. cinerea, H. eximia, H. geographica, H. granosa, H. microcephala, H. miotympanum, H. tuberculosa, and H. versicolor groups are also demonstrably nonmonophyletic. Hylinae is composed of four major clades. The first of these includes the Andean stream-breeding Hyla, Aplastodiscus, all Gladiator Frogs, and a Tepuian clade. The second clade is composed of the 30-chromosome Hyla, Lysapsus, Pseudis, Scarthyla, Scinax (including the H. uruguaya group), Sphaenorhynchus, and Xenohyla. The third major clade is composed of Nyctimantis, Phrynohyas, Phyllodytes, and all South American/West Indian casque-headed frogs: Aparasphenodon, Argenteohyla, Corythomantis, Osteocephalus, Osteopilus, Tepuihyla, and Trachycephalus. The fourth major clade is composed of most of the Middle American/Holarctic species groups of Hyla and the genera Acris, Anotheca, Duellmanohyla, Plectrohyla, Pseudacris, Ptychohyla, Pternohyla, Smilisca, and Triprion. A new monophyletic taxonomy mirroring these results is presented where Hylinae is divided into four tribes. Of the species currently in Hyla, 297 of the 353 species are placed in 15 genera; of these, 4 are currently recognized, 4 are resurrected names, and 7 are new. Hyla is restricted to H. femoralis and the H. arborea, H. cinerea, H. eximia, and H. versicolor groups, whose contents are redefined. Phrynohyas is placed in the synonymy of Trachycephalus, and Pternohyla is placed in the synonymy of Smilisca. The genus Dendropsophus is resurrected to include all former species of Hyla known or suspected to have 30 chromosomes. Exerodonta is resurrected to include the former Hyla sumichrasti group and some members of the former H. miotympanum group. Hyloscirtus is resurrected for the former Hyla armata, H. bogotensis, and H. larinopygion groups. Hypsiboas is resurrected to include several species groups - many of them redefined here - of Gladiator Frogs. The former Hyla albofrenata and H. albosignata complexes of the H. albomarginata group are included in Aplastodiscus. New generic names are erected for (1) Agalychnis calcarifer and A. craspedopus; (2) Osteocephalus langsdorffii; the (3) Hyla aromatica, (4) H. bromeliacia, (5) H. godmani, (6) H. mixomaculata, (7) H. taeniopus, (8) and H. tuberculosa groups; (9) the clade composed of the H. pictipes and H. pseudopuma groups; and (10) a clade composed of the H. circumdata, H. claresignata, H. martinsi, and H. pseudopseudis groups. Copyright © American Museum of Natural History 2005.

Organização celular dos testículos em Hylidae e leptodactylidae, no Pantanal (Estado do Mato Grosso do Sul, Brasil)

The objective of this work was to make a comparative analysis of germ cell organization at different stages of cellular differentiation in adult males of Dendropsophus nanus (Boulenger, 1889), Pseudis limellum (Cope, 1862), P. paradoxa (Linnaeus, 1758), and Scinax acuminatus (Cope, 1862), belonging to the family Hylidae; and Leptodactylus chaquensis (Cei, 1950) and L. podicipinus (Cope, 1862), belonging to the family Leptodactylidae, collected in the Pantanal and in Serra da Bodoquena, Mato Grosso do Sul State, Brazil. The testes were removed and fixed, dehydrated in a graded series of ethanol and embedded in methacrylate glycol resin (Historesin Leica®). The sections were stained by 1% toluidine blue and observed under light microscope. It was detected that all individual of the Hylidae family show, throughout the year, the presence of all germ cell types of spermatogenesis. However, all Leptodactylidae family individuals only show the presence of all germ cell types during the rainy season. The variations of characteristics in seminiferous epithelium organization, as well as the evident difference in the amount of spermatozoa inside the tubules, are evidence that the anurans in this work show different forms of spermatogenesis development throughout the year: the cycle is continuous for the Hylidae family, and discontinuous with explosive release of spermatozoa for the Leptodactylidae family.

Expression of acid phosphatase in the seminiferous epithelium of vertebrates

Acid phosphatases (AcPs) are known to provide phosphate to tissues that have high energy requirements, especially during development, growth and maturation. During spermatogenesis AcP activity is manifested in heterophagous lysosomes of Sertoli cells. This phagocytic function appears to be hormone-independent. We examined the expression pattern of AcP during the reproductive period of four species belonging to different vertebrate groups: Tilapia rendalli (Teleostei, Cichlidae), Dendropsophus minutus (Amphibia, Anura), Meriones unguiculatus (Mammalia, Rodentia), and Oryctolagus cuniculus (Mammalia, Lagomorpha). To demonstrate AcP activity, cryosections were processed for enzyme histochemistry by a modification of the method of Gömöri. AcP activity was similar in the testes of these four species. Testes of T. rendalli, D. minutus and M. unguiculatus showed an intense reaction in the Sertoli cell region. AcP activity was detected in the testes of D. minutus and O. cuniculus in seminiferous epithelium regions, where cells are found in more advanced stages of development. The seminiferous epithelium of all four species exhibited AcP activity, mainly in the cytoplasm of either Sertoli cells or germ cells. These findings reinforce the importance of AcP activity during the spermatogenesis process in vertebrates. © FUNPEC-RP.

Relaciones entre los rotíferos, copépodos planctónicos ciclopoides y la calidad del agua en dos embalses Brasileños

Planktonic rotifers and cyclopoid copepods were studied in two reservoirs of different trophic states (eutrophic and oligo/mesoeutrophic) in the south of Brazil. During a year, monthly samplings were carried out in three stations in each reservoir. Species richness, frequency and abundance were used to find out useful and indicatives trends of water quality based on these organisms, reinforced by literature data. Species that showed higher differences between reservoirs were chosen. For Rotifera, richness, frequency and abundance of Brachionus were higher in the eutrophic reservoir, but Plationus patulus occurred only in the oligo/mesotrophic reservoir. For copepods, Tropocyclops prasinus dominated in the eutrophic reservoir, but Thermocyclops decipiens, T. minutus, T. inversus and Microcyclops anceps were dominants in the oligo/mesotrophic reservoir. In the canonical correspondence analysis, these species were indicators of the trophic state and were related with chlorophyll-a, total phytoplankton and total phosphorus. The use of these species can be efficient in the studied regions (subtropical/temperate), but comparing with other Brazilian reservoirs of tropical climate, the results could be different. Despite the dominance of T. decipiens over T. minutus, T. inversus has been widely used in Brazil as an indicator of eutrophic waters; in those cases of excessive eutrophication, other species, more rustic, commonly dominate. In the present study, Thermocyclops was dominant in the oligo/mesotrophic reservoir. The dominance of Brachionus for rotifers and Tropocyclops prasinus and Acanthocyclops robustus for copepods were indicative of eutrophic conditions.

Uso de hábitat, micro-hábitat e coexistência com predadores em taxocenoses de girinos de anuros no noroeste paulista

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Variação no tamanho corpóreo e cantos de anúncio de hilídeos (ANURA) na Serra do Mar

Pós-graduação em Biologia Animal - IBILCE

Ecologia de uma comunidade de anuros em Botucatu, SP

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)