Copia de carta escrita por Don Pablo de Luna y Azcarate al Exmo. Sr. Marqués de Almonacid y Conde de Pavía en que se refiere la avenida e inundación del rio Ebro y el daño que ha hecho en la ciudad de Çaragoça [Manuscrito]

Hay un ejemplar encuadernado con: XVIII/1830

[Copia de documentos relativos a Franciscus Carolus de Borja et Centelles, Marchio de Lombai...] [Manuscrito]

Enc. piel con hierros dorados deteriorada

Copia del iurament que presten los iurats de la Ciutat de Valencia, en lo principi, y ans de la administracio de sos oficis, y dels capitols, ordinacions y estatuts kalendariats en dit iurament [Texto impreso]

Port con esc. xil. de la Ciudad

Copia de vna carta que escribiò el licenciado Antonio de Castro, à vn cavallero amigo suyo, de la Ciudad de Valecia, remitiendole el papel adjunto [Texto impreso]

Contiene de p. 3 a 20 : Respuesta al escandaloso papel theologico.

Copia de una carta que se escriue de Ierusalem al M.R.P.F. Gabriel Alcalâ, Comissario en el Reyno de Valencia de la Tierra Santa, dandole auiso de los trabajos que aora de nueuo estân padeciendo los religiosos en la Ciudad Santa [Texto impreso]

Autor tomado de final de texto

Paso á V.S. la adjunta copia, para que por ella se entere de la Resolución del Rey en orden á la solicitud del Gremio de Albañiles ... [Texto impreso]

Hay un ejemplar encuadernado con: Real Cedula con las nuevas ordenanzas que su magestad... y... su Real Junta General de Comercio, Moneda y Minas concede a la congregacion colegio y arte de Plateros de la ciudad... de Valencia... en 28 de Abril de 1761. (XVIII/3734).

[Copia del Bando de Fernando VII de 1790 prohibiendo la petición de limosnas en el Reino de Valencia, Murcia, Provincia de la Mancha y otros territorios agregados, publicado en la Audiencia de Valencia. Certificado por D. Vicente Esteve] [Texto impreso]

Hay un ejemplar encuadernado con: La Real Compañia formada por S.M. para llevar a efecto el canal de navegación y riego del reyno de Murcia, las... experiencias de que informa D. Domingo Aguirre... le han confirmado en los abusos y fraudes que hacen muchos... (XVIII/4279).

[Copia de la providencia dada por la Sala del Crimen del Reino de Valencia mandando que los corregidores y alcaldes publiquen por bando las Pragmáticas de armas prohibidas y vigilen su cumplimiento. Certificado por D. José Mariano Morató]

Hay un ejemplar encuadernado con: Pragmatica que su Magestad ha mandado promulgar revalidando los anteriores en qu ese prohibe el uso de armas blancas cortas y las de fuego ... (XVIII/4280).

Copia de dos cartas escritas por Dn. Joseph Perez de Montoro al Sacrista y Canonigo de Xativa su paisano [Manuscrito] : que parecio ponerlas aqui (aunque en prosa) paraque sirviessen como de aprovacion, epistola y Prologo a sus agudezas...

Manuscrito encuadernado con: "Concilia Magnae Britanniae et Hiberniae a Synodo Verolamiensi... "(NP001-009/1)

SIRE-1, a copia/Ty1-like retroelement from soybean, encodes a retroviral envelope-like protein

The soybean genome hosts a family of several hundred, relatively homogeneous copies of a large, copia/Ty1-like retroelement designated SIRE-1. A copy of this element has been recovered from a Glycine max genomic library. DNA sequence analysis of two SIRE-1 subclones revealed that SIRE-1 contains a long, uninterrupted, ORF between the 3′ end of the pol ORF and the 3′ long terminal repeat (LTR), a region that harbors the env gene in retroviral genomes. Conceptual translation of this second ORF produces a 70-kDa protein. Computer analyses of the amino acid sequence predicted patterns of transmembrane domains, α-helices, and coiled coils strikingly similar to those found in mammalian retroviral envelope proteins. In addition, a 65-residue, proline-rich domain is characterized by a strong amino acid compositional bias virtually identical to that of the 60-amino acid, proline-rich neutralization domain of the feline leukemia virus surface protein. The assignment of SIRE-1 to the copia/Ty1 family was confirmed by comparison of the conceptual translation of its reverse transcriptase-like domain with those of other retroelements. This finding suggests the presence of a proretrovirus in a plant genome and is the strongest evidence to date for the existence of a retrovirus-like genome closely related to copia/Ty1 retrotransposons.

Naturally occurring variation in copia expression is due to both element (cis) and host (trans) regulatory variation.

Significant differences in levels of copia [Drosophila long terminal repeat (LTR) retrotransposon] expression exist among six species representing the Drosophila melanogaster species complex (D. melanogaster, Drosophila mauritiana, Drosophila simulans, Drosophila sechellia, Drosophila yakuba, and Drosophila erecta) and a more distantly related species (Drosophila willistoni). These differences in expression are correlated with major size variation mapping to putative regulatory regions of the copia 5' LTR and adjacent untranslated leader region (ULR). Sequence analysis indicates that these size variants were derived from a series of regional duplication events. The ability of the copia LTR-ULR size variants to drive expression of a bacterial chloramphenicol acetyltransferase reporter gene was tested in each of the seven species. The results indicate that both element-encoded (cis) and host-genome-encoded (trans) genetic differences are responsible for the variability in copia expression within and between Drosophila species. This finding indicates that models purporting to explain the dynamics and distribution of retrotransposons in natural populations must consider the potential impact of both element-encoded and host-genome-encoded regulatory variation to be valid. We propose that interelement selection among retrotransposons may provide a molecular drive mechanism for the evolution of eukaryotic enhancers which can be subsequently distributed throughout the genome by retrotransposition.

The genomic and physical organization of Ty1-copia-like sequences as a component of large genomes in Pinus elliottii var. elliottii and other gymnosperms.

A DNA sequence, TPE1, representing the internal domain of a Ty1-copia retroelement, was isolated from genomic DNA of Pinus elliottii Engelm. var. elliottii (slash pine). Genomic Southern analysis showed that this sequence, carrying partial reverse transcriptase and integrase gene sequences, is highly amplified within the genome of slash pine and part of a dispersed element >4.8 kbp. Fluorescent in situ hybridization to metaphase chromosomes shows that the element is relatively uniformly dispersed over all 12 chromosome pairs and is highly abundant in the genome. It is largely excluded from centromeric regions and intercalary chromosomal sites representing the 18S-5.8S-25S rRNA genes. Southern hybridization with specific DNA probes for the reverse transcriptase gene shows that TPE1 represents a large subgroup of heterogeneous Ty1-copia retrotransposons in Pinus species. Because no TPE1 transcription could be detected, it is most likely an inactive element--at least in needle tissue. Further evidence for inactivity was found in recombinant reverse transcriptase and integrase sequences. The distribution of TPE1 within different gymnosperms that contain Ty1-copia group retrotransposons, as shown by a PCR assay, was investigated by Southern hybridization. The TPE1 family is highly amplified and conserved in all Pinus species analyzed, showing a similar genomic organization in the three- and five-needle pine species investigated. It is also present in spruce, bald cypress (swamp cypress), and in gingko but in fewer copies and a different genomic organization.