The distribution and spread of citrus canker in Emerald, Australia

Citrus canker is a disease of citrus and closely related species, caused by the bacterium Xanthomonas citri subsp. citri. This disease, previously exotic to Australia, was detected on a single farm [infested premise-1, (IP1). IP is the terminology used in official biosecurity protocols to describe a locality at which an exotic plant pest has been confirmed or is presumed to exist. IP are numbered sequentially as they are detected] in Emerald, Queensland in July 2004. During the following 10 months the disease was subsequently detected on two other farms (IP2 and IP3) within the same area and studies indicated the disease first occurred on IP1 and spread to IP2 and IP3. The oldest, naturally infected plant tissue observed on any of these farms indicated the disease was present on IP1 for several months before detection and established on IP2 and IP3 during the second quarter (i.e. autumn) 2004. Transect studies on some IP1 blocks showed disease incidences ranged between 52 and 100% (trees infected). This contrasted to very low disease incidence, less than 4% of trees within a block, on IP2 and IP3. The mechanisms proposed for disease spread within blocks include weather-assisted dispersal of the bacterium (e.g. wind-driven rain) and movement of contaminated farm equipment, in particular by pivot irrigator towers via mechanical damage in combination with abundant water. Spread between blocks on IP2 was attributed to movement of contaminated farm equipment and/or people. Epidemiology results suggest: (i) successive surveillance rounds increase the likelihood of disease detection; (ii) surveillance sensitivity is affected by tree size; and (iii) individual destruction zones (for the purpose of eradication) could be determined using disease incidence and severity data rather than a predefined set area.

Citrus Information Kit. Agrilink, your growing guide to better farming guide

Each Agrilink kit has been designed to be both comprehensive and practical. As the kits are arranged to answer questions of increasing complexity, they are useful references for both new and experienced producers of specific crops. Agrilink integrates the technology of horticultural production with the management of horticultural enterprises. REPRINT INFORMATION - PLEASE READ! For updated information please call 13 25 23 or visit the website www.daf.qld.gov.au This publication has been reprinted as a digital book without any changes to the content published in 1997. We advise readers to take particular note of the areas most likely to be out-of-date and so requiring further research: see detailed information on first page of the kit. Even with these limitations we believe this information kit provides important and valuable information for intending and existing growers. This publication was last revised in 1997. The information is not current and the accuracy of the information cannot be guaranteed by the State of Queensland. This information has been made available to assist users to identify issues involved in the production of citrus. This information is not to be used or relied upon by users for any purpose which may expose the user or any other person to loss or damage. Users should conduct their own inquiries and rely on their own independent professional advice. While every care has been taken in preparing this publication, the State of Queensland accepts no responsibility for decisions or actions taken as a result of any data, information, statement or advice, expressed or implied, contained in this publication.

CSIRO lead National Citrus Scion Breeding Program

National Citrus Scion Breeding Program.

Protecting the Australian citrus industry from HLB (greening) disease

Protecting the Australian citrus industry from HLB (greening) disease.

National Citrus Triploid Breeding

This project advances commercially desirable citrus selections that have resilient seedlessness. It builds on existing expertise and develops germplasm resources in the utilisation of interploid crossing for the production of new triploid hybrids with outstanding fruit quality. Advanced germplasm will progress toward commercialisation with fruit displays, and the production of a final generation of trees for semi-commercial plantings. At the opposite end of the breeding spectrum, new triploid hybrids will be produced. Growers will see triploid citrus from their national breeding project for the first time, providing a window on future new varieties that will emerge from the pipe-line of germplasm that has been developed through past project investment.

Phyllosticta spp. on cultivated Citrus in Australia

The occurrence of pathogenic and endophytic species of Phyllosticta on cultivated Citrus in Australia was investigated by DNA sequence analysis of specimens held in plant pathology herbaria and culture collections. Sequences of the internal transcribed spacer region (ITS1, 5.8S, ITS2), and partial translation elongation factor 1-alpha (TEF) gene of 41 Phyllosticta-like isolates from Citrus were compared to those sequences from the type specimens of Phyllosticta recorded from around the world. Phylogenetic analysis resolved all the sequences of Australian accessions into two major clades. One clade corresponded to P. citricarpa, which causes citrus black spot disease. The other clade contained P. capitalensis, which is a known endophyte of Citrus and many other plant species. All included herbarium accessions previously designated as Guignardia mangiferae are now designated P. capitalensis. No Australian isolates were identified as the newly described pathogens of citrus P. citriasiana or P. citrichinaensis, or the endophytes Guignarida mangiferae, P. brazilianiae, or P. citribraziliensis. © 2013 Australasian Plant Pathology Society Inc.

First Fruiting Intergeneric Hybrids between Citrus and Citropsis

Fruiting hybrids are reported for the first time between the genera Citrus L. and Citropsis (Engl.) Swing. & M.Kell. Conventional hybridization using the recently described species Citrus wakonai P.I.Forst. & M.W.Sm. and Citropsis gabunensis (Engl.) Swing. & M.Kell. resulted in high rates of fruit set and seed formation. Although seed were only half normal size, over 90% germinated without the need for embryo rescue techniques. Plant losses were high during the first few months but after six months, the 327 surviving hybrids were potted on. These grew vigorously on their own roots and 35 of them flowered within two years of sowing. Plants flowered continuously but all were pollen-sterile and ovaries abscised shortly after petal fall. However, at 25 months, two newly flowering hybrids began setting fruit. The development, identification, morphology, breeding efficiency, and future implications of this unique germplasm are described.

Novel Pathotypes of Elsinoe australis Associated with Citrus australasica and Simmondsia chinensis in Australia

Molecular phylogenetic analysis, morphology and pathogenicity to citrus fruit were used to study two isolates of Elsinoe australis associated with scab-like symptoms on a fruit of Citrus australasica (finger lime) and Simmondsia chinensis (jojoba) in Australia. In addition to being associated with finger lime, the isolate from finger lime could cause scab symptoms on C. x aurantium cv. Murcott tangor in pathogenicity tests, but could not cause scab symptoms on the other orange, mandarin, lemon or grapefruit tested. Pathogenicity tests also support previous studies showing the isolate from jojoba could not produce symptoms on fruit of C. natsudaidai. Based on the findings of this study, two novel pathotypes of E. australis are designated from Australia; namely the Finger Lime (FL) pathotype associated with finger lime, and the Jojoba Black Scab (JBS) pathotype associated with black scab of jojoba. The significance of these novel E. australis pathotypes on market access and biosecurity issues for citrus are briefly discussed.

Biomimetic synthesis of silver nanoparticles by Citrus limon (lemon) aqueous extract and theoretical prediction of particle size

In the present study silver nanoparticles were rapidly synthesized at room temperature by treating silver ions with the Citrus limon (lemon) extract The effect of various process parameters like the reductant con centration mixing ratio of the reactants and the concentration of silver nitrate were studied in detail In the standardized process 10(-2) M silver nitrate solution was interacted for 411 with lemon Juice (2% citric acid concentration and 0 5% ascorbic acid concentration) in the ratio of 1 4(vol vol) The formation of silver nanoparticles was confirmed by Surface Plasmon Resonance as determined by UV-Visible spectra in the range of 400-500 nm X ray diffraction analysis revealed the distinctive facets (1 1 1 200 220 2 2 2 and 3 1 1 planes) of silver nanoparticles We found that citric acid was the principal reducing agent for the nanosynthesis process FT IR spectral studies demonstrated citric acid as the probable stabilizing agent Silver nanoparticles below 50 nm with spherical and spheroidal shape were observed from transmission electron microscopy The correlation between absorption maxima and particle sizes were derived for different UV-Visible absorption maxima (corresponding to different citric acid concentrations) employing MiePlot v 3 4 The theoretical particle size corresponding to 2% citric acid concentration was corn pared to those obtained by various experimental techniques like X ray diffraction analysis atomic force microscopy and transmission electron microscopy (C) 2010 Elsevier B V All rights reserved

Heat mediated synthesis of silver nanoparticles using citrus limon (lemon) extract

In the present study, silver nanoparticles were rapidly synthesized by treating silver ions with Citrus limon (lemon) extract at higher temperature. The effect of process parameters like reductant concentration, mixing ratio of the reactants, concentration of silver nitrate and heating time period were studied. The formation of silver nanoparticles was confirmed by surface plasmon resonance as determined by UV-visible spectra in the range of 400-500 nm. X-ray diffraction analysis revealed the distinctive facets (111, 200, 220, 222 and 311 planes) of silver nanoparticles. Nanoparticles below 50 nm with spherical and spheroidal shape were observed from microscopic studies. The study offers a rapid method to synthesize silver nanoparticles within ten minutes of interaction with the bio-reductant.

Induccion del celo en vacas en anestro post parto mediante la administracion de sales minerales, vitaminas y masajes ovaricos. Realizada en el municipio de Rio Blanco, departamento de Matagalpa

El presente estudio se realizó con el objetivo de establecer la efectividad del tratamiento de Inducción al celo de vacas en anestro post parto por medio de la administración de sales minerales, vitaminas y masajes ovarios; dicho tratamiento fue llevado a cabo en el municipio de Río Blanco departamento de Matagalpa. Caracterizándose por una buena distribución de las lluvias la mayor parte del año. El dicho municipio se da una predominancia de una cultura de gran alta productividad ganadera, el tamaño requerido de la muestra de este trabajo de 20 hembras en periodo de anestro post parto mayor de 50 días, las cuales fueron seleccionadas al azar y evalua das clínicamente por palpación rectal por un profesional de la medicina veterinaria. Los datos fueron analizados mediante la prueba de chi –cuadrado habiendo obtenido un resultado significativo a un nivel de confianza del 95% entre los tratamientos. Con base en los resultados obtenidos posterior al tratamiento de la administración de sales minerales, vitamina y masaje ovárico se alcan zó una respuesta positiva del 80 % al celo, el 80% en tasa de preñez y en relación a la aparición del celo posteri or al tratamiento se alcanzo una media de 6.13 días que al comparar la efectividad del tratamiento de solo la aplicación de masaje ovárico se observo un buen resultado positivo, superando las expectativas. En comparación con las hembras tratadas con solo masaje ovárico se obtuvo una respuesta positiva al celo de un 60%, con un 30% de tasa de preñez y 7.33 días promedio en anestro posterior al tratamiento, por lo tanto elaboramos la hipótesis basándonos en el estadígrafo de Chi-cuadrado obteniendo un resultado significativo empleando un nivel de significancia del 0.05% por lo que atribuimos las diferencias de ambos tratamientos.

Caracterización del manejo zootécnico del cerdo criollo (Sus scrofa domesticus) en el área rural del municipio de Río Blanco, Matagalpa, 2013

El cerdo fue introducido a Nicaragua en el año 1493, durante el segundo viaje que Cristóbal Colón realizara a América durante la colonización española. La crianza de cerdo de traspatio en el país juega un rol importante en la economía de las familias rural es, siendo característica la presencia de animales criollos. Estos animales representan un recurso adaptado a condiciones adversas de crianza. El presente trabajo se realizó en el municipio de Río Blanco departamento de Matagalpa, con el objetivo de caracterizar el manejo zootécnico del cerdo criollo. Para recabar la información se diseñó un instrumento con preguntas abiertas y cerradas (encuesta) que incluyó los componentes: socioeconómico, alimentación, salud, genética , producción y reproducción, mediante un sondeo previo. Se realizaron entrevistas al nivel de tod o el sector rural del municipio, utilizando el criterio de selección de la cría de al menos 3 cerdos, las comunidades incluidas fueron un total de 20 con un total de 68 productores y se registrar con 548 cerdos. Del total de productores el 52.94% de encuestados eran mujeres y el 47.05% eran hombres, con un promedio de edad de 43.73 años; según el estudio, el papel de la mujer en la actividad de crianza de cerdos criollos en la zona rural es impactante, pues alcanza el 98% como principal ocupación. En lo que respecta a la alimentación de los cerdos en el municipio , esta tiene como base granos básicos, tubérculos y musáceas, además de los desperdicios de cocina. La base genética de los cerdos en el municipio está constituida principalmente por grupos raciales de animales criollos con características diversas en cuanto a su exterior. Según los productores en el último año se registró un 39. 71% de muertes, mientras que el 60. 29 afirmó lo contrario, el 34. 37% de muertes ocurre por trastornos de la salud, un 34 . 37% por causas desconocidas, un 21. 87% por plastamientos y un 9. 37% por diarrea. Las categorías de cerdos lactantes y en desarrollo son las más propensas a trastornos de la salud con un 56% cada un a, la categoría de cerdos en crecimiento con un 32%, cerdas adultas y engorde con 24% y cerdos adultos con 16%. En las cerdas reproductoras se estimó según la información brindada, que incorporan a la cerda hacia los 6.51 meses, obteniendo un promedio de 1. 87 partos por año, con una prolificidad de 8.11 cerditos por camada con una viabilidad del 88.26% que equivale a 7. 16 cerditos por camada al destete. De manera general, se observó que para mejorar la producción de cerdos en la zona, no es necesario reemplazar totalmente a los cerdos criollos por razas mejoradas, sino que hay que mejorar las condiciones de crianza y manejo que hasta el momento se les ofrece

Determinación de la actividad radicular del Lima tahiti citrus aurantifolia (christm,) swing, mediante la variación de almacenamiento del agua del suelo, utilizando técnicas nucleares

Con el objetivo de determinar la actividad radicular y el patrón de distribución de las raíces activas del lima Tahití Citrus aurantifolia (Christm.) Swing., mediante la variación de almacenamiento de agua del suelo utilizando técnicas nucleares, se condujo un experimento en la finca San Bernardo, municipio de Masatepe, Departamento de Masaya. Se escogieron plantas de la misma edad, pureza varietal, características vegetativas uniformes y se establecieron en cada unidad experimental 4 tratamientos (50, 100, 150,200 cm de distancia del tronco), por 2 repeticiones para un total de 16 observaciones y se cubrió con plástico de polietileno negro en un área de 19.63 m2 que corresponde a un circulo de radio igual a 250 cm del tronco a cada árbol. En la época seca se tomaron lecturas, y la información obtenida y analizada permite concluir que en árboles de limón Tahití de 6 años de edad, y en las condiciones edafoclimáticas y de manejo del cultivo en que se llevó el experimento, presenta su actividad radicular directamente proporcional a las distancias horizontales del tronco del árbol, mediante el consumo total de agua en el volumen de suelo (O - 90 cm de profundidad), y a la vez directamente proporcional cada 30 cm de profundidad para un perfil de suelo de (O - 90 cm de profundidad), lo que indica presencia de un sistema radicular extenso y profundo.