Aspectos epidemiológicos da tuberculose na região de saúde de Botucatu-SP, 1993 a 1998

Pós-graduação em Saúde Coletiva - FMB

Identificação e caracterização de um gene cry recombinante de Bacillus thuringiensis var. londrina

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

A utilização do Resource Description and Access (RDA) na criação de registros de autoridade para pessoas, famílias e entidades coletivas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Biossegurança na clínica de radiologia odontológica

In the practice of dental radiology, although there was no production of aerosols, there is the contamination of equipment and surfaces by contact with blood and saliva. This is making necessary the adoption infection control procedures to protect patients and staff. The objective of this paper is to present the procedures of prevention during the radiographic examination and radiographic processing in the darkroom. The proposed recommendations for the area of Dental Radiology include efficient cleaning and protection of equipment and surfaces, personal protective measures and to avoid contamination of the darkroom. These recommendations are obtained by simple procedures as cover films and radiological equipment with plastic barriers.

Ensino de ecoico, tato e mando: uma revisão bibliográfica dos artigos do Journal of Applied Behavior Analysis (JABA)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

A MULTILEVEL MODEL TO ADDRESS BATCH EFFECTS IN COPY NUMBER ESTIMATION USING SNP ARRAYS

Submicroscopic changes in chromosomal DNA copy number dosage are common and have been implicated in many heritable diseases and cancers. Recent high-throughput technologies have a resolution that permits the detection of segmental changes in DNA copy number that span thousands of basepairs across the genome. Genome-wide association studies (GWAS) may simultaneously screen for copy number-phenotype and SNP-phenotype associations as part of the analytic strategy. However, genome-wide array analyses are particularly susceptible to batch effects as the logistics of preparing DNA and processing thousands of arrays often involves multiple laboratories and technicians, or changes over calendar time to the reagents and laboratory equipment. Failure to adjust for batch effects can lead to incorrect inference and requires inefficient post-hoc quality control procedures that exclude regions that are associated with batch. Our work extends previous model-based approaches for copy number estimation by explicitly modeling batch effects and using shrinkage to improve locus-specific estimates of copy number uncertainty. Key features of this approach include the use of diallelic genotype calls from experimental data to estimate batch- and locus-specific parameters of background and signal without the requirement of training data. We illustrate these ideas using a study of bipolar disease and a study of chromosome 21 trisomy. The former has batch effects that dominate much of the observed variation in quantile-normalized intensities, while the latter illustrates the robustness of our approach to datasets where as many as 25% of the samples have altered copy number. Locus-specific estimates of copy number can be plotted on the copy-number scale to investigate mosaicism and guide the choice of appropriate downstream approaches for smoothing the copy number as a function of physical position. The software is open source and implemented in the R package CRLMM available at Bioconductor (http:www.bioconductor.org).

Pre-clinical in vivo models for the screening of bone biomaterials for oral/craniofacial indications: focus on small-animal models.

Preclinical in vivo experimental studies are performed for evaluating proof-of-principle concepts, safety and possible unwanted reactions of candidate bone biomaterials before proceeding to clinical testing. Specifically, models involving small animals have been developed for screening bone biomaterials for their potential to enhance bone formation. No single model can completely recreate the anatomic, physiologic, biomechanic and functional environment of the human mouth and jaws. Relevant aspects regarding physiology, anatomy, dimensions and handling are discussed in this paper to elucidate the advantages and disadvantages of small-animal models. Model selection should be based not on the 'expertise' or capacities of the team, but rather on a scientifically solid rationale, and the animal model selected should reflect the question for which an answer is sought. The rationale for using heterotopic or orthotopic testing sites, and intraosseous, periosseous or extraskeletal defect models, is discussed. The paper also discusses the relevance of critical size defect modeling, with focus on calvarial defects in rodents. In addition, the rabbit sinus model and the capsule model in the rat mandible are presented and discussed in detail. All animal experiments should be designed with care and include sample-size and study-power calculations, thus allowing generation of meaningful data. Moreover, animal experiments are subject to ethical approval by the relevant authority. All procedures and the postoperative handling and care, including postoperative analgesics, should follow best practice.

Trends in mean and extreme precipitation in the Mount Kenya region from observations and reanalyses

Land and water management in semi-arid regions requires detailed information on precipitation distribution, including extremes, and changes therein. Such information is often lacking. This paper describes statistics of mean and extreme precipitation in a unique data set from the Mount Kenya region, encompassing around 50 stations with at least 30 years of data. We describe the data set, including quality control procedures and statistical break detection. Trends in mean precipitation and extreme indices calculated from these data for individual rainy seasons are compared with corresponding trends in reanalysis products. From 1979 to 2011, mean precipitation decreased at 75% of the stations during the ‘long rains’ (March to May) and increased at 70% of the stations during the ‘short rains’ (October to December). Corresponding trends are found in the number of heavy precipitation days, and maximum of consecutive 5-day precipitation. Conversely, an increase in consecutive dry days within both main rainy seasons is found. However, trends are only statistically significant in very few cases. Reanalysis data sets agree with observations with respect to interannual variability, while correlations are considerably lower for monthly deviations (ratios) from the mean annual cycle. While some products well reproduce the rainfall climatology and some the spatial trend pattern, no product reproduces both.

Cross-culture perspectives on fear of HIV disease and AIDS in health care workers

Health care workers have been known to carry into the workplace a variety of judgmental and negative attitudes towards their patients. In no other area of patient care has this issue been more pronounced as in the management of patients with AIDS. Health care workers have refused to treat or manage patients with AIDS and have often treated them more harshly than identically described leukemia patients. Some health care institutions have simply refused to admit patients with AIDS and even recent applicants to medical colleges and schools of nursing have indicated a preference for schools in areas with low prevalence of HIV disease. Since the attitudes of health care workers do have significant consequences on patient management, this study was carried out to determine the differences in clinical practice in Nigeria and the United States of America as it relates to knowledge of a patient's HIV status, determine HIV prevalence and culture in each of the study sites and how they impact on infection control practices, determine the relationship between infection control practices and fear of AIDS, and also determine the predictors of safe infection control practices in each of the study sites.^ The study utilized the 38-item fear of AIDS scale and the measure of infection control questionnaire for its data. Questionnaires were administered to health care workers at the university teaching hospital sites of Houston, Texas and Calabar in Nigeria. Data was analyzed using a chi-square test, and where appropriate, a student t-tests to establish the demographic variables for each country. Factor analysis was done using principal components analysis followed by varimax rotation to simple structure. The subscale scores for each study site were compared using t-tests (separate variance estimates) and utilizing Bonferroni adjustments for number of tests. Finally, correlations were carried out between infection control procedures and fear of AIDS in each study site using Pearson-product moment correlation coefficients.^ The study revealed that there were five dimensions of the fear of AIDS in health care workers, namely fear of loss of control, fear of sex, fear of HIV infection through blood and illness, fear of death and medical interventions and fear of contact with out-groups. Fear of loss of control was the primary area of concern in the Nigerian health care workers whereas fear of HIV infection through blood and illness was the most important area of AIDS related feats in United States health care workers. The study also revealed that infection control precautions and practices in Nigeria were based more on normative and social pressures whereas it was based on knowledge of disease transmission, supervision and employee discipline in the United States, and thus stresses the need for focused educational programs in health care settings that emphasize universal precautions at all times and that are sensitive to the cultural nuances of that particular environment. ^

Macrobenthic abundance, biomass, productivity and production in the deep Arctic ocean

The few existing studies on macrobenthic communities of the deep Arctic Ocean report low standing stocks, and confirm a gradient with declining biomass from the slopes down to the basins as commonly reported for deep-sea benthos. In this study we have further investigated the relationship of faunal abundance (N), biomass (B) as well as community production (P) with water depth, geographical latitude and sea ice concentration. The underlying dataset combines legacy data from the past 20 years, as well as recent field studies selected according to standardized quality control procedures. Community P/B and production were estimated using the multi-parameter ANN model developed by Brey (2012). We could confirm the previously described negative relationship of water depth and macrofauna standing stock in the Arctic deep-sea. Furthermore, the sea-ice cover increasing with high latitudes, correlated with decreasing abundances of down to < 200 individuals/m**2, biomasses of < 65 mg C/m**2 and P of < 75 mg C/m**2/y. Stations under influence of the seasonal ice zone (SIZ) showed much higher standing stock and P means between 400 - 1400 mg C/m**2/y; even at depths up to 3700 m. We conclude that particle flux is the key factor structuring benthic communities in the deep Arctic ocean, explaining both the low values in the ice-covered Arctic basins and the high values along the SIZ.

Physical oceanography from eight Seaglider missions in the Mediterranean Sea, Eastern Basin

In 2008, the Oceanography Center at the University of Cyprus acquired two underwater gliders in the framework of a nationally-managed infrastructure upgrade program. The gliders were purchased from the Seaglider Fabrication Center at the University of Washington. Both gliders are rated to 1000 m and carry a typical sensor payload: non-pumped conductivity-temperature-depth sensors (CTD), a dissolved oxygen sensor, an optical triplet to measure optical backscatter at 400 nm, 700 nm, and chlorophyll-a fluorescence. Since March of 2009, the gliders have been used in a long-term observing program of the Cypriot EEZ, and by September 2015, have covered more than 15300 km over ground and 3500 dive cycles in 940 glider days. Butterfly patterns have been flown in two configurations, either on the western or eastern side of the EEZ south of Cyprus. The glider endurance lines criss-cross the region in order to more accurately locate and investigate the mesoscale structures south of Cyprus, and in particular the Cyprus eddy which is often the dominant feature. Based on the near real time observations, the glider mission was sometimes altered in order to more fully sample the Cyprus eddy, or to locate its center or extent. A summary of the raw and processed data collected, and the quality control procedures are presented, in order for future users to take advantage of this unique data set.

Caracterización y modelado de grandes centrales fotovoltaicas

La presente tesis doctoral propone un conjunto de ensayos de corta duración destinados a cubrir la ausencia de estándares internacionales específicamente adaptados a la tecnología y al panorama fotovoltaico actual que indiquen como realizar los procedimientos de control de calidad para comprobar que las grandes centrales fotovoltaicas ejecutadas responden a las expectativas establecidas durante la fase de proyecto. Dichos ensayos buscan, desde el punto de vista estrictamente técnico, obtener en un corto periodo de tiempo (típicamente una semana) resultados altamente repetitivos y representativos del comportamiento de la instalación bajo análisis, a la vez que minimizar al máximo la incertidumbre global, aspectos fundamentales para los procedimientos de control general de la calidad de una central. Los ensayos propuestos comprueban tanto el comportamiento general de la central, en términos de su capacidad de producción energética, como el de sus principales componentes, generadores fotovoltaicos e inversores, en términos de potencia máxima y eficiencia, respectivamente. También se aconseja una revisión de la calidad y seguridad de la instalación y de los materiales empleados en la ejecución de la central para evitar un envejecimiento prematuro de los mismos. Todos los ensayos recogidos en el texto se apoyan en la experiencia recopilada por el “Grupo de Sistemas Fotovoltaicos del Instituto de Energía Solar de la Universidad Politécnica de Madrid”, que ha estado involucrado en procedimientos de control de calidad de unas 50 centrales fotovoltaicas, con una potencia acumulada cercana a 250 MW, la mayoría de ellas instaladas en España. ABSTRACT This PhD thesis proposes a set of short-duration tests to establish quality control procedures to ensure that large photovoltaic plants fulfil the initial expectations. The motivation for this work is the lack of international standards specifically adapted to the present photovoltaic technology and its state of the art. From a strict technical point of view, these tests seek to obtain highly repetitive and representative results about the behaviour of the installation under study in a short period of time (typically one single week); and to minimize the global uncertainty. These are the two keys aspects required in quality control procedures. The proposed tests evaluate the general behaviour of the photovoltaic plants, in terms of energy production, as well as the particular behaviour of their main devices, photovoltaic arrays and inverters, in terms of maximum power and efficiency, respectively. A review of the installation quality and safety, and the employed materials in its execution to avoid premature aging is also recommended. The tests here presented are based on the experience accumulated by the “Grupo de Sistemas Fotovoltaicos del Instituto de Energia Solar de la Universidad Politecnica de Madrid”. This group has been involved in quality control procedures of about 50 photovoltaic plants, with an accumulated power close to 250 MW, most of them installed in Spain.

A quality assessment system for fresh fruits in hypermarkets and fruit trading centres.

When the fresh fruit reaches the final markets from the suppliers, its quality is not always as good as it should, either because it has been mishandled during transportation or because it lacks an adequate quality control at the producer level, before being shipped. This is why it is necessary for the final markets to establish their own quality assessment system if they want to ensure to their customers the quality they want to sell. In this work, a system to control fruit quality at the last level of the distribution channel has been designed. The system combines rapid control techniques with laboratory equipment and statistical sampling protocols, to obtain a dynamic, objective process, which can substitute advantageously the quality control inspections carried out visually by human experts at the reception platform of most hypermarkets. Portable measuring equipment have been chosen (firmness tester, temperature and humidity sensors...) as well as easy-to-use laboratory equipment (texturometer, colorimeter, refractometer..,) combining them to control the most important fruit quality parameters (firmness, colour, sugars, acids). A complete computer network has been designed to control all the processes and store the collected data in real time, and to perform the computations. The sampling methods have been also defined to guarantee the confidence of the results. Some of the advantages of a quality assessment system as the proposed one are: the minimisation of human subjectivity, the ability to use modern measuring techniques, and the possibility of using it also as a supplier's quality control system. It can be also a way to clarify the quality limits of fruits among members of the commercial channel, as well as the first step in the standardisation of quality control procedures.

Contributions to uncertainty reduction in the estimation of PV plants performance

Esta tesis doctoral presenta un procedimiento integral de control de calidad en centrales fotovoltaicas, que comprende desde la fase inicial de estimación de las expectativas de producción hasta la vigilancia del funcionamiento de la instalación una vez en operación, y que permite reducir la incertidumbre asociada su comportamiento y aumentar su fiabilidad a largo plazo, optimizando su funcionamiento. La coyuntura de la tecnología fotovoltaica ha evolucionado enormemente en los últimos años, haciendo que las centrales fotovoltaicas sean capaces de producir energía a unos precios totalmente competitivos en relación con otras fuentes de energía. Esto hace que aumente la exigencia sobre el funcionamiento y la fiabilidad de estas instalaciones. Para cumplir con dicha exigencia, es necesaria la adecuación de los procedimientos de control de calidad aplicados, así como el desarrollo de nuevos métodos que deriven en un conocimiento más completo del estado de las centrales, y que permitan mantener la vigilancia sobre las mismas a lo largo del tiempo. Además, los ajustados márgenes de explotación actuales requieren que durante la fase de diseño se disponga de métodos de estimación de la producción que comporten la menor incertidumbre posible. La propuesta de control de calidad presentada en este trabajo parte de protocolos anteriores orientados a la fase de puesta en marcha de una instalación fotovoltaica, y las complementa con métodos aplicables a la fase de operación, prestando especial atención a los principales problemas que aparecen en las centrales a lo largo de su vida útil (puntos calientes, impacto de la suciedad, envejecimiento…). Además, incorpora un protocolo de vigilancia y análisis del funcionamiento de las instalaciones a partir de sus datos de monitorización, que incluye desde la comprobación de la validez de los propios datos registrados hasta la detección y el diagnóstico de fallos, y que permite un conocimiento automatizado y detallado de las plantas. Dicho procedimiento está orientado a facilitar las tareas de operación y mantenimiento, de manera que se garantice una alta disponibilidad de funcionamiento de la instalación. De vuelta a la fase inicial de cálculo de las expectativas de producción, se utilizan los datos registrados en las centrales para llevar a cabo una mejora de los métodos de estimación de la radiación, que es la componente que más incertidumbre añade al proceso de modelado. El desarrollo y la aplicación de este procedimiento de control de calidad se han llevado a cabo en 39 grandes centrales fotovoltaicas, que totalizan una potencia de 250 MW, distribuidas por varios países de Europa y América Latina. ABSTRACT This thesis presents a comprehensive quality control procedure to be applied in photovoltaic plants, which covers from the initial phase of energy production estimation to the monitoring of the installation performance, once it is in operation. This protocol allows reducing the uncertainty associated to the photovoltaic plants behaviour and increases their long term reliability, therefore optimizing their performance. The situation of photovoltaic technology has drastically evolved in recent years, making photovoltaic plants capable of producing energy at fully competitive prices, in relation to other energy sources. This fact increases the requirements on the performance and reliability of these facilities. To meet this demand, it is necessary to adapt the quality control procedures and to develop new methods able to provide a more complete knowledge of the state of health of the plants, and able to maintain surveillance on them over time. In addition, the current meagre margins in which these installations operate require procedures capable of estimating energy production with the lower possible uncertainty during the design phase. The quality control procedure presented in this work starts from previous protocols oriented to the commissioning phase of a photovoltaic system, and complete them with procedures for the operation phase, paying particular attention to the major problems that arise in photovoltaic plants during their lifetime (hot spots, dust impact, ageing...). It also incorporates a protocol to control and analyse the installation performance directly from its monitoring data, which comprises from checking the validity of the recorded data itself to the detection and diagnosis of failures, and which allows an automated and detailed knowledge of the PV plant performance that can be oriented to facilitate the operation and maintenance of the installation, so as to ensure a high operation availability of the system. Back to the initial stage of calculating production expectations, the data recorded in the photovoltaic plants is used to improved methods for estimating the incident irradiation, which is the component that adds more uncertainty to the modelling process. The development and implementation of the presented quality control procedure has been carried out in 39 large photovoltaic plants, with a total power of 250 MW, located in different European and Latin-American countries.

Diseño arquitectónico y protección en caso de incendio: desarrollo normativo español en materia de evacuación en los siglos XIX y XX

Esta es la búsqueda de respuestas a esa duda constante: De dónde venimos y que hemos ido dejando por el camino. ¿Está todo claro en este recorrido o hemos actuado por acumulación de errores heredados de procesos anteriores? Es la investigación a través del descubrimiento de nuestro pasado, de nuestros orígenes en materia de seguridad de protección contra incendios, y sobre todo de ejecución de una arquitectura pensada para ser recorrida con mayor seguridad y ser evacuada en un tiempo razonable. El trabajo investiga, a nivel nacional, la evolución de la sociedad y sus efectos sobre la manera de interpretar el problema de la seguridad contra incendios en los edificios. El interés fundamentalmente es poner en claro todos aquellos aspectos que afectan a la evacuación de las personas. Para ello se han estudiado los principales hitos de actuación, las preocupaciones principales surgidas en cada momento y las soluciones adoptadas. Se ha comprobado su aplicación o su demora hasta que se ha producido el siguiente gran suceso que ha motivado una nueva revisión de los procedimientos de diseño y control. En primer lugar, tratando de relacionar los incendios fundamentales que han influido en nuestra forma de abordar el problema en distintos momentos de la historia de España. En segundo lugar, haciendo un recorrido sobre la figura del arquitecto y su participación en los medios de control y legislación sobre la manera de intervenir en el servicio de incendios o de resolver los edificios en materia de protección contra incendios o evacuación de sus ocupantes. En definitiva, descubriendo los escritos de algunos especialistas, fundamentales para entender nuestra manera de abordar el problema de la protección en los edificios, a lo largo de la historia. Se ha revisado como se han producido los siniestros más importantes en teatros y otros locales públicos .Analizando la forma en que los arquitectos implicados han tratado de resolver las posibles deficiencias ante el riesgo. Se trata de la tipología edificatoria donde, por primera vez, surge la preocupación por adoptar medidas y procedimientos de seguridad en caso de incendio. Resultan locales con una importante siniestralidad, donde se desarrolla la principal actividad lúdica del momento, y que por el importante número de personas que albergan, son fuente de preocupación entre el público y las autoridades. Otras cuestiones en un tema tan amplio, que quedan simplemente esbozadas en este trabajo de investigación, son los procedimientos de los sistemas de extinción, la estructura organizativa de la ciudad, las primeras sociedades de seguros de incendios, la aparición de patentes a partir del desarrollo industrial del siglo XIX. Todo ello, con el hilo conductor de la reglamentación que se ha ido elaborando al respecto. Al principio, sobre espectáculos públicos, acotando el punto de partida en la materia de nuestra reglamentación. Anticipando sistemas constructivos y datos dimensionales de la evacuación. Llegados a mediados del siglo XX, abordando otros usos. Haciendo seguimiento de la modernización de los procesos edificatorios y la reglamentación sectorial. Recabando información de las organizaciones profesionales que comienzan a reclamar una coordinación nacional de los sistemas preventivos y que desemboca en el Proyecto de Reglamento de prevención contra el fuego que nunca será publicado. Toda esta etapa, plagada de documentos de carácter voluntario u obligatorio, local y nacional, van definiendo los criterios dimensionales con los que debe resolverse los elementos arquitectónicos susceptibles de servir para la evacuación. Se trata de una etapa intensa en documentación, cambiante, sujeta a los criterios que establecen los países del entorno más avanzados en la materia. Las dos últimas décadas del siglo, acotadas por la transición política y varios siniestros de graves consecuencias, definen el proceso normativo que culmina con el código técnico de la edificación que hoy conocemos. Es un periodo de aprendizaje y asimilación del capítulo de la seguridad, donde los métodos son variados. Donde la intencionalidad última es trasladar un sistema prescriptivo a un modelo prestacional propio de la madurez en el análisis del tema y en las corrientes de los países del entorno. ABSTRACT This is the search for answers to that constant question: Where do we come from and what have left along the way? Has everything been clear on this journey, or have we acted as a result of a collection of errors learned from prior processes? This has been research through exploration of our past, of our origins regarding fire protection safety, and, above all, of the endeavour to utilize architecture aimed at offering the highest level of safety and evacuation in a reasonable time. This project has researched society’s change nationwide and its effects on how to interpret the difficulty of fire protection safety in buildings. Its focus has fundamentally been to clarify those aspects that affect the evacuation of people. To this end, the main milestones of action, the principal concerns that have arisen at each step, and the solutions taken have all been studied. A check was performed on their application; or their delay until a significant event occurred that prompted a new revision of design and control procedures. Firstly, this was done by attempting to connect the main fires that have influenced how we handle the problem at different times in Spain’s history. Secondly, an examination was done on the figure of the architect and his participation in the means of control and legislation on how to intercede in fire services, or how the architect finds solutions for buildings in terms of fire protection, or the evacuation of their occupants. In short, the written works of certain specialists, who are essential to our understanding of how to deal with the problem of protection in buildings, were explored throughout history. A study was done on the most significant disasters in theatres and other public establishments. This was done by analysing the way the architects involved have aimed to solve possible points liable to risk. It is a classification of building where, for the first time, the concern arose to adopt safety measures and procedures in the event of fires. Public establishments with considerable accident rates emerged. Here the main entertainment activities of the time took place. These spaces were a source of worry among the public and authorities due to the high number of persons they accommodated. Other issues in such an extensive subject, which are only outlined in this research study, are procedures in extinguishing systems, the organizational structure of cities, the first fire insurance companies, and the appearance of patents after the industrial development of the 19th century. All of these aspects are joined by the common thread of regulations that have been created in this matter. At the beginning, these regulations were for public shows, thus defining the starting point for our regulations. This was done in anticipation of structural systems and size data of evacuations. With the arrival of the mid-20th century, different uses were addressed. A modernization of construction processes and the industry regulations were tracked. Information was gathered from professional organizations that began to demand a national coordination of prevention systems which led to the Regulation Project on fire prevention which will never be published. Throughout this stage, replete with voluntary and compulsory documents, both on the local and national level, the dimensional criteria to be used in the resolution of architectural elements open to use in evacuation were defined. This was a period that was filled with documentation, change, and subject to the criteria that the most advanced countries in the field established in this regard. The final two decades of the century, marked by political transition and several accidents with grave consequences, defined the regulation process that culminated with the building technical code known today. This was a period of learning and understanding in the chapter of safety, where the methods are varied. In this chapter, the ultimate goal is to insert a prescriptive-based standard into a performance-based code suitable for cultivated experience in analysis of the subject and the tendencies in countries dealing with this field.