Abundance of mesozooplankton from nine cruises in Northern Adriatic Sea during NA64

The NA64-Mesozooplankton dataset contains biogeochemistry and mesozooplankton data collected in a series of 9 cruises in the Northern Adriatic completed from January 1965 to September 1965 monthly, and December 1965. Biogeochemistry sampling was undertaken using 5L Nansen bottles fired at 0m, 5m, 10m, 20m, 30m and/or bottom depths. The dataset includes 709 samples analysed for nitrate, phosphate, temperature, salinity and density. Mesozooplankton sampling was undertaken at the same locations as for biogeochemistry, using two different net (Hensen non-closing and Appstein closing net). The dataset includes 146 samples analysed for mesozooplankton composition (at higher taxonomic level), abundance and volume settlement. After sedimentation and volume measurement, the fish larva and fish eggs were extracted from samples (egss of Engraulis encrasicholus were determined). Chaetognaths were partly isolated. Identification at higher taxonomic level of zooplankters was completed. Taxonomic identification was done at Smithonian Mediterranean Centre in Salambo. After sedimentation and volume measurement, the fish larva and fish eggs were extracted from samples (egss of Engraulis encrasicholus were determined). Chaetognaths were partly isolated. Identification at higher taxonomic level of zooplankters was completed. Taxonomic identification was done at Smithonian Mediterranean Centre in Salambo.

Enzyme activity, body measures and heart mass of Sepia officinalis sampled in the English Channel and Adriatic Sea

In the eurythermal cuttlefish Sepia officinalis, performance depends on hearts that ensure systemic oxygen supply over a broad range of temperatures. We therefore aimed to identify adjustments in energetic cardiac capacity and underlying mitochondrial function supporting thermal acclimation and adaptation that could be crucial for the cuttlefish's competitive success in variable environments. Two genetically distinct cuttlefish populations were acclimated to 11, 16 and 21°C. Subsequently, skinned and permeabilised heart fibres were used to assess mitochondrial functioning by means of high-resolution respirometry and a substrate-inhibitor protocol, followed by measurements of cardiac citrate synthase and cytosolic enzyme activities. Temperate English Channel cuttlefish had lower mitochondrial capacities but larger hearts than subtropical Adriatic cuttlefish. Warm acclimation to 21°C decreased mitochondrial complex I activity in Adriatic cuttlefish and increased complex IV activity in English Channel cuttlefish. However, compensation of mitochondrial capacities did not occur during cold acclimation to 11°C. In systemic hearts, the thermal sensitivity of mitochondrial substrate oxidation was high for proline and pyruvate but low for succinate. Oxygen efficiency of catabolism rose as temperature changed from 11 to 21°C via shifts to oxygen-conserving oxidation of proline and pyruvate and via reduced relative proton leak. The changes observed for substrate oxidation, mitochondrial complexes, relative proton leak and heart mass improve energetic efficiency and essentially seem to extend tolerance to high temperatures and reduce associated tissue hypoxia. We conclude that cuttlefish sustain cardiac performance and, thus, systemic oxygen delivery over short- and long-term changes of temperature and environmental conditions by multiple adjustments in cellular and mitochondrial energetics.

Biogeochemistry from nine cruises in Northern Adriatic Sea during NA64

The NA64-Mesozooplankton dataset contains biogeochemistry and mesozooplankton data collected in a series of 9 cruises in the Northern Adriatic completed from January 1965 to September 1965 monthly, and December 1965. Biogeochemistry sampling was undertaken using 5L Nansen bottles fired at 0m, 5m, 10m, 20m, 30m and/or bottom depths. The dataset includes 709 samples analysed for nitrate, phosphate, temperature, salinity and density. Mesozooplankton sampling was undertaken at the same locations as for biogeochemistry, using two different net (Hensen non-closing and Appstein closing net). The dataset includes 146 samples analysed for mesozooplankton composition (at higher taxonomic level), abundance and volume settlement. Temperature was measured with a standard oceanographic thermometers. Salinity was determined by titration after Mohr-Knudsen using standardised water I.C.E.S. Copenhagen with 0,01 permil accuracy. Density was calculated using the following equation Sigma-t = T - (sigma 0 + 0,1324) 1 - At + Bt (sigma 0 - 0,1324). Phosphate samples for the determination of nutrients were collected in 500 ml and filtrated through 0,3 µm membrane filter. 3ml of cloroform was added to stabilize the samples. They were analysed after few days in the laboratory on land. Nitrate samples for the determination of nutrients were collected in 500 ml and filtrated through 0,3 µm membrane filter. 3ml of cloroform was added to stabilize the samples. They were analysed after few days in the laboratory on land.