Fluorescent microplate-based analysis of protein-DNA interactions II:immobilized DNA

A simple protein-DNA interaction analysis has been developed using both a high-affinity/high-specificity zinc finger protein and a low-specificity zinc finger protein with nonspecific DNA binding capability. The latter protein is designed to mimic background binding by proteins generated in randomized or shuffled gene libraries. In essence, DNA is immobilized onto the surface of microplate wells via streptavidin capture, and green fluorescent protein (GFP)-labeled protein is added in solution as part of a crude cell lysate or protein mixture. After incubation and washing, bound protein is detected in a standard microplate reader. The minimum sensitivity of the assay is approximately 0.4 nM protein. The assay format is ideally suited to investigate the interactions of DNA binding proteins from within crude cell extracts and/or mixtures of proteins that may be encountered in protein libraries generated by codon randomization or gene shuffling.

“Se le dé papel, prensa y letras”. Albert O’Farail y el libro misionero "The Life of the Virgin Marie" (1671-1693)

El proyecto tipográfico del irlandés Albert O’Farail se concibió como un libro misionero destinado a la empresa confesional que la monarquía de España desarrollaba en las Islas Británicas desde comienzos del Seiscientos. Las traducciones de distintos tratados doctrinales y teológicos, representados por su devoción particular a la Virgen María, procuraban acercar la religión a los católicos del Norte a través de mecanismos de proximidad cultural como complemento a la predicación de los ministros espirituales patrocinados por Carlos II. El empeño por dar a las prensas esta miscelánea de obras, financiada por la corona y compuesta por el arte de su propio autor, discurrió en paralelo a la querella abierta por su esposa, María Manuela Laínez, para reivindicar sus derechos nobiliarios al título marquesal de Mayo. Tanto en Madrid como en Roma, la imbricación de ambos negociados determinó su malograda resolución.

Med det falska och det ärligä ögat: (en bok om en människa II) af Adolf Paul

Alanimeke kannessa ja esiössä: en reflexstudie.

El hombre perdido: Ramón Gómez de la Serna y Albert Camus

[es] Se establece aquí una relación entre dos obras narrativas publicadas durante los años posteriores a la Segunda Guerra Mundial: El hombre perdido (1947), de Ramón Gómez de la Serna, y La chute (1956), de Albert Camus. Se establece la relación en el plano del pensamiento filosófico y político y se señalan las coincidencias entre las obras. La ciudad, el paseante solitario, el distanciamiento del «gregarismo» y la defensa del individuo coinciden con un leitmotiv, el suicidio, que se da insistentemente en los años cuarenta como consecuencia del desasosiego contemporáneo, la guerra, el nazismo y el totalitarismo socialista impuesto en la URSS, defendido en numerosos núcleos de intelectuales europeos y americanos. [en] This article deals with the connection between two prose works published during the years after World War ii: El hombre perdido (1947) by Ramón Gómez de la Serna and La chute (1956) by Albert Camus. The article examines the relationship from a philosophical and political perspective and establishes the coincidences in both writers’ works. The city, the solitary stroller, the distance from «gregariousness» and the defense of the individual coincide with a leitmotiv, suicide, which has been insistently present during the 1940s as a consequence of contemporary unease, war, Nazism and the socialist totalitarianism imposed on the USSR and supported by many European and American intellectual groups.

Numerical approximation of a fractional-in-space diffusion equation (II) - with nonhomogeneous boundary conditions

In this paper, a space fractional di®usion equation (SFDE) with non- homogeneous boundary conditions on a bounded domain is considered. A new matrix transfer technique (MTT) for solving the SFDE is proposed. The method is based on a matrix representation of the fractional-in-space operator and the novelty of this approach is that a standard discretisation of the operator leads to a system of linear ODEs with the matrix raised to the same fractional power. Analytic solutions of the SFDE are derived. Finally, some numerical results are given to demonstrate that the MTT is a computationally e±cient and accurate method for solving SFDE.

Flop II

An installation of sculptural objects that combine references to the portrait bust, drapery and socle with quotidian and incidental objects from the artist's studio. The work form part of the artist's ongoing self-portrait project in which he enacts formal strategies for tempering the authority conveyed by the self-portrait as an artistic genre.

PSED II and the comprehensive Australian study of entrepreneurial emergence (CAUSEE)

Two current longitudinal studies in advanced countries, PSED II in the US and CAUSEE in Australia, have attempted to harmonize the major features of the research design. A comparison of the initial screening and first detailed interviews indicates a higher participation in new firm creation in the U.S. Similar types of persons are involved in both countries, albeit more immigrants, older individuals with more work experience and more established individuals in Australia. The nascent enterprises in the two countries are similar on many characteristics, although those in Australia report greater emphasis on new technology and international customers. Assessment of the prevalence of nascent enterprises and new firms from the Global Entrepreneurship Monitor surveys indicates a higher prevalence of new firms in Australia. These two longitudinal projects may help determine if this reflects a high proportion of new firm births or greater survival in the early years among Australian new firms.