Mesenchymal stromal cells up-regulate CD39 and increase adenosine production to suppress activated T-lymphocytes

Mesenchymal stromal cells (MSCs) suppress T cell responses through mechanisms not completely understood. Adenosine is a strong immunosuppressant that acts mainly through its receptor A(2a) (ADORA2A). Extracellular adenosine levels are a net result of its production (mediated by CD39 and CD73), and of its conversion into inosine by Adenosine Deaminase (ADA). Here we investigated the involvement of ADO in the immunomodulation promoted by MSCs. Human T lymphocytes were activated and cultured with or without MSCs. Compared to lymphocytes cultured without MSCs, co-cultured lymphocytes were suppressed and expressed higher levels of ADORA2A and lower levels of ADA. In co-cultures, the percentage of MSCs expressing CD39, and of T lymphocytes expressing CD73, increased significantly and adenosine levels were higher. Incubation of MSCs with media conditioned by activated T lymphocytes induced the production of adenosine to levels similar to those observed in co-cultures, indicating that adenosine production was mainly derived from MSCs. Finally, blocking ADORA2A signaling raised lymphocyte proliferation significantly. Our results suggest that some of the immunomodulatory properties of MSCs may, in part, be mediated through the modulation of components related to adenosine signaling. These findings may open new avenues for the development of new treatments for GVHD and other inflammatory diseases. (C) 2011 Elsevier B.V. All rights reserved.

Substitution of crude cell wall for neutral detergent fibre in the equations of the Cornell Net Carbohydrate and Protein System that predict carbohydrate fractions: application to sunflower (Helianthus annulus L.)

Prediction of carbohydrate fractions using equations from the Cornell Net Carbohydrate and Protein System (CNCPS) is a valuable tool to assess the nutritional value of forages. In this paper these carbohydrate fractions were predicted using data from three sunflower (Helianthus annuus L.) cultivars, fresh or as silage. The CNCPS equations for fractions B(2) and C include measurement of ash and protein-free neutral detergent fibre (NDF) as one of their components. However, NDF lacks pectin and other non-starch polysaccharides that are found in the cell wall (CW) matrix, so this work compared the use of a crude CW preparation instead of NDF in the CNCPS equations. There were no differences in the estimates of fractions B, and C when CW replaced NDF; however there were differences in fractions A and B2. Some of the CNCPS equations could be simplified when using CW instead of NDF Notably, lignin could be expressed as a proportion of DM, rather than on the basis of ash and protein-free NDF, when predicting CNCPS fraction C. The CNCPS fraction B(1) (starch + pectin) values were lower than pectin determined through wet chemistty. This finding, along with the results obtained by the substitution of CW for NDF in the CNCPS equations, suggests that pectin was not part of fraction B(1) but present in fraction A. We suggest that pectin and other non-starch polysaccharides that are dissolved by the neutral detergent solution be allocated to a specific fraction (B2) and that another fraction (B(3)) be adopted for the digestible cell wall carbohydrates.

The net benefit of saving the Asian Elephant: A policy and contingent valuation Study

Reports results from a contingent valuation survey of willingness to pay for the conservation of the Asian elephant of a sample of urban residents living in three selected housing schemes in Colombo, the capital of Sri Lanka. Face–to–face surveys were conducted using an interview schedule. A non-linear logit regression model is used to analyse the respondents’ responses for the payment principle questions and to identify the factors that influence their responses. We investigate whether urban residents’ willingness to pay for the conservation of elephants is sufficient to compensate farmers for the damage caused by elephants. We find that the beneficiaries (the urban residents) could compensate losers (the farmers in the areas affected by human–elephant conflict) and be better off than in the absence of elephants in Sri Lanka. Therefore, there is a strong economic case for the conservation of the wild elephant population in Sri Lanka. However, we have insufficient data to determine the optimal level of this elephant population in the Kaldor-Hicks sense. Nevertheless, the current population of elephant in Sri Lanka is Kaldor-Hicks preferable to having none.

Comiss??o de ??tica Setorial: os desafios de fazer cumprir a ??tica na administra????o p??blica federal

Este texto parte do reconhecimento de que a implementa????o de Comiss??es de ??tica Setoriais constitui componente fundamental para concretizar a promo????o da ??tica no ??mbito do Sistema de Gest??o da ??tica do Poder Executivo Federal. Entretanto, essa n??o ?? uma tarefa simples. A an??lise de dados secund??rios e a revis??o da literatura sobre o tema suscitam as quest??es: como implementar Comiss??es de ??tica Setoriais? H?? lacunas no processo de implanta????o? Quais seriam os meios para transpor as dificuldades inerentes a esse processo? Realizou-se pesquisa explorat??ria, indutiva, qualitativa. O estudo de dois casos (um de sucesso e outro de fracasso no processo de implementa????o dessas Comiss??es) indica que a ado????o sistem??tica de instrumentos pedag??gicos para a promo????o da ??tica, o comprometimento da alta administra????o e a cria????o de uma rede interna de pessoas capacitadas e sens??veis ao tema s??o elementos importantes nesse processo. O estudo sugere, ainda, a????es que podem favorecer a efetiva implementa????o dessas Comiss??es.

Architectural prototyping: from CCS to .Net

Over the last decade, software architecture emerged as a critical issue in Software Engineering. This encompassed a shift from traditional programming towards software development based on the deployment and assembly of independent components. The specification of both the overall systems structure and the interaction patterns between their components became a major concern for the working developer. Although a number of formalisms to express behaviour and to supply the indispensable calculational power to reason about designs, are available, the task of deriving architectural designs on top of popular component platforms has remained largely informal. This paper introduces a systematic approach to derive, from CCS behavioural specifications the corresponding architectural skeletons in the Microsoft .Net framework, in the form of executable C and Cω code. The prototyping process is fully supported by a specific tool developed in Haskell

Prototyping Behavioural Specifications in the.NET framework

Over the last decade, software architecture emerged as a critical design step in Software Engineering. This encompassed a shift from traditional programming towards the deployment and assembly of independent components. The specification of the overall system structure, on the one hand, and of the interactions patterns between its components, on the other, became a major concern for the working developer. Although a number of formalisms to express behaviour and supply the indispensable calculational power to reason about designs, are available, the task of deriving architectural designs on top of popular component platforms has remained largely informal. This paper introduces a systematic approach to derive, from behavioural specifications written in Ccs, the corresponding architectural skeletons in the Microsoft .Net framework in the form of executable C] code. Such prototyping process is automated by means of a specific tool developed in Haskell

Rootstocks resistant to Meloidogyne incognita and compatibility of grafting in net melon

Due to the few studies about grafting in net melon, in order to obtain better control of soil pathogens, the aim of the present study was to evaluate 16 genotypes of Cucurbitaceae: Benincasa hispida, Luffa cylindrica, pumpkin 'Jacarezinho', pumpkin 'Menina Brasileira', squash 'Exposição', squash 'Coroa', pumpkin 'Canhão Seca', pumpkin 'Squash', pumpkin 'Enrrugado Verde', pumpkin 'Mini Paulista', pumpkin 'Goianinha', watermelon 'Charleston Gray', melon 'Rendondo Gaucho', melon 'Redondo Amarelo', cucumber 'Caipira HS' and cucumber 'Caipira Rubi', regarding to compatibility of grafting in net melon and resistance to Meloidogyne incognita, based on the reproduction factor (RF), according to Oostenbrink (1966). To assess resistance, the seedlings were transplanted to ceramic pots and inoculated with 300/mL eggs and/or second stage juveniles of M. incognita. At 50 days after transplanting, the plants were removed from the pots and the resistance was evaluated. The compatibility between resistant rootstock and grafts of net melon was determined by performing simple cleft grafting, in a commercial net melon hybrid of great market acceptance and susceptible to M. incognita (Bonus no. 2). The genotypes Luffa cylindrica, pumpkin 'Goianinha', pumpkin 'Mini-Paulista', melon 'Redondo Amarelo', watermelon 'Charleston Gray' are resistant to the nematode M. incognita. The better compatibilities occurred with the rootstocks melon 'Amarelo', which presented 100% of success, followed by pumpkin 'Mini-Paulista' with 94%. On the other hand, Sponge gourd, watermelon 'Charleston Gray' and pumpkin 'Goianinha' showed low graft take percentages of 66%, 62% and 50%, respectively.

Shame and Guilt

O autor explora a lenda de Adão e Eva, contada no livro de Milton “Paraíso Perdido”, e o fenómeno da vergonha e da culpa, a sua ligação com a religião e o seu lugar e significância na sociedade humana.

O ensino colonial destinado aos "indígenas" de Angola

Revista Lusófona de Educação

Fio por fio, o bordado se constrói : as representações de gênero em livros didáticos de língua portuguesa : das 7.ª e 8.ª séries

O estudo das representações de gênero nos livros didáticos de Língua Portuguesa constitui a moldura desta dissertação de Mestrado. Objetivamos, nesse sentido, analisar os livros mais adotados nas escolas públicas do Estado da Bahia, para o ensino de 7ª e 8ª séries no período de 2000 a 2006. Nesta pesquisa, realizamos um estudo das teorias de gênero e dos múltiplos enfoques conceituais desse termo, a partir de uma perspectiva histórica. Mostramos a proposta oficial de trabalho, apresentado pelos PCNs, para o desenvolvimento da Orientação Sexual nas escolas, bem como o percurso histórico das políticas governamentais brasileiras que legitimam a importância do livro didático como instrumento de ensino-aprendizagem. A pesquisa está baseada em uma abordagem qualitativa, sendo interpretados os fragmentos textuais e as ilustrações a partir da análise de conteúdo. Os dados estão separados em sete categorias, definidas de acordo com o número de incidência em que foram representados os homens e as mulheres em situações cotidianas. Os resultados estão estruturados em duas seções: a primeira trata sobre as representações de gênero no espaço público e a segunda,essas representações no ambiente familiar. Percebemos que, tanto na esfera pública como no ambiente familiar, os textos e as ilustrações inseridas nos livros didáticos ainda representam os gêneros em papéis dicotomizados. Os espaços definidos para o homem e a mulher, bem como as obrigações de ambos para com a família, ainda se estão identificando com princípios tradicionais. Desta forma, a visão de relacionamento estabelecida para os gêneros associa-se à concepção patriarcal. Esta constatação demonstra a importância de que os usuários dos livros didáticos precisam questionar e ressignificar as representações de gênero que são veiculadas por esse recurso de ensino-aprendizagem.

Ler em tempo livre durante o Estado Novo: A função do suplemento infantil Pim-Pam-Pum na formação da criança

RESUMO: Esta dissertação tem como objectivo compreender a influência da ideologia do Estado Novo nos conteúdos inseridos no suplemento infantil Pim-Pam-Pum. Visa ainda relacionar, num determinado momento temporal o suplemento infantil de um dos jornais mais lidos da época O Século, com a ideologia do Estado Novo. Procura identificar os valores incutidos nos números a analisar no Pim-Pam-Pum num período de dois anos. O período histórico em análise do suplemento infantil Pim-Pam-Pum compreende os exemplares entre 1953 a 1955. Como principais conclusões do estudo retira-se que o suplemento infantil procurava educar o seu público de forma lúdica. Por outro lado, transmitia e incutia em colaboração com a família os principais valores salazaristas. A imprensa escrita passa a ser uma arma de propaganda ideológica do Estado Novo numa tentativa de conter informação desfavorável ao regime perante as pressões internas e externas. Com a institucionalização da censura após a segunda Guerra Mundial, a vigilância e o exame das publicações dos organismos competentes em colaboração com a própria auto-censura dos jornalistas, simplificou-se. No entanto a imprensa procurou cumprir com a sua missão de divulgação cultural e lúdica através de jornais diários, revistas e suplementos infantis. ABSTRACT:The aim of this dissertation is to understand the influence of the ideology of the "Estado Novo" in the contents inserted in the children's supplement Pim-Pam-Pum. It also aims to relate, in a certain historical period, the children’s supplement of one of the most important newspapers of the time, "O Século", with the ideology of the "Estado Novo". In addition, it intends to identify the values ingrained in the numbers to be analysed in Pim-Pam-Pum in a period of two years. The period under analysis of the children's supplement Pim-Pam-Pum includes the numbers from 1953 to 1955. One of the main conclusions of this study is that the children's aforementioned supplement aimed to educate its audience in a joyful manner. On the other hand, it transmitted as well as inculcated with the families’ collaboration the main Salazarist values. The press becomes a weapon of ideological propaganda of the "Estado Novo" in an attempt to keep harmful information to the regime from the internal and external pressures. With the institucionalization of censorship after the Second World War, the careful surveillance of publications by the competent and relevant organisms in collaboration with the censorship made by the journalists themselves, was simplified. However, the press tried to accomplish its mission of cultural and playful diffusion by means of newspapers, magazines and children's supplements.