940 resultados para 5-35
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在分析延安市宝塔区、安塞县南部 7乡镇的沟壑密度、布坝密度、现有坝地面积占耕地面积比重的基础上 ,提出了该区今后发展坝地的规模及淤地坝建设模式
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以 2a生中国沙棘为试验材料 ,研究了土壤干旱胁迫下沙棘苗木水分生理状况 ,内源GA1 3 及ABA水平与萌芽率的关系 ,结果表明 :土壤干旱胁迫导致苗体含水量、水势、自由水含量下降 ,显著提高冬季休眠与春季萌动期的内源ABA水平而降低内源GA1 3 含量 ,GA1 3 ABA降低 ,较晚达到萌动所需的调控阈值 ,萌芽延迟。重度胁迫下苗木延迟达 2 5d且萌芽后生长缓慢。长期土壤中度干旱条件下沙棘苗萌芽期亦略有延迟 ,萌芽后生长略有抑制
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为了探讨城市森林三维绿量的测算方式及精度,该文利用2001年8月沈阳市真彩航片和抽样调查,借助ARC/GIS,以“立体量推算立体量”的方法测算沈阳城市森林三维绿量.结果表明,不同城市森林类型的单位面积三维绿量以风景游憩林最高,为5.35 m3/m2,附属林4.93 m3/m2,道路林3.65 m3/m2,生态公益林3.62 m3/m2,生产经营林2.35m3/m2;城市森林分布区的单位面积三维绿量为4.25 m3/m2;城区单位面积城市森林三维绿量为0.35 m3/m2.沈阳城市森林总体三维绿量为161 296 716.85 m3,其中附属林为60 116 966.36 m3,占37.27%,生态公益林43 321 771.43 m3,占26.86%,风景游憩林30 482 879.04 m3,占18.90%,道路林23 841 208.75 m3,占14.78%,生产经营林3 533 891.27 m3,占2.19%.经检验,该方法精度达到85.50%(α=0.05).
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本文从水源保护林经营目标、经营原则出发,结合国际林业发展新趋势和国内林业建设需要,将现代恢复生态学原理、林隙动态理论、有效干扰理论和边缘效应原理引入防护林经营当中,提出定向经营的新观点,揭示定向恢复及其规律,为实现我国防护林健康更新、持续利用开拓新思路,提供新方法。
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在实验室条件下 ,将不同含水量的 3种森林类型的土柱分别置于 0、5、1 5、2 5和 35℃条件下 ,进行土壤呼吸测定 .结果表明 ,在 0~ 35℃范围内 ,土壤呼吸速率与温度呈正相关 .在一定含水量范围内 (0 .2 1~ 0 .37kg·kg- 1 ) ,土壤呼吸随含水量的增加而升高 ,当含水量超出该范围 ,土壤呼吸速率则随含水量的变化而降低 .土壤温度和水分对土壤呼吸作用存在明显的交互作用 .不同森林类型土壤呼吸作用强弱存在显著差异 ,大小顺序为阔叶红松林 >岳桦林 >云冷杉暗针叶林 .阔叶红松林土壤呼吸作用的最佳条件是土壤温度 35℃、含水量 0 .37kg·kg- 1 ;云冷杉暗针叶林下的山地棕色针叶林土壤呼吸作用的最佳条件是 2 5℃、0 .2 1kg·kg- 1 ;岳桦林土壤呼吸作用的最佳条件是 35℃、含水量 0 .37kg·kg- 1 .但是 ,由于长白山阔叶红松林、云冷杉林和岳桦林处在不同的海拔带上 ,同期不同森林类型土壤温度各不相同 ,相差 4~ 5℃ ,所以野外所测的同期山地棕色针叶林土呼吸速率应低于暗棕色森林土呼吸速率 ,山地生草森林土呼吸速率应高于山地棕色针叶林土的呼吸速率
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合成了新的双核配合物 [Ni(OXPN) Ni(phen) 2 ](Cl O4) 2 · 2 H2 O[OXPN=N,N′-双 - (3-氨丙基 )草酰胺 ],并进行了表征 .测定了对应单晶 [Ni(OXPN) Ni(phen) 2 ](Cl O4) 2 · 2 H2 O· CH3OH的结构及变温磁化率 (4~ 30 0 K) ,表明该配合物属 P1 空间群 ,a=0 .96 6 5 (4) nm,b=1.32 71(7) nm,c=1.716 3(5 ) nm,α=90 .42 (3)°,β=94.12 (3)°,γ=110 .6 4(4)°,V=2 .0 5 35 5 nm3.两个镍原子配位环境分别为平面四边形和畸变八面体构型 .以草酰二胺桥联的 Ni- Ni核间相距 0 .5 2 6 2 nm.磁化率测定表明两个镍原子间没有磁交换作用 ,说明一个镍原子处于平面四边形配位环境
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A soluble polymer emitting green color with high efficiency was synthesized. Bright green electroluminescence devices, both single layer and multilayer, were fabricated. The luminous efficiency was improved dramatically. Carrier injection from the electrodes to the emissive layer and concomitant green electroluminescence from the emissive layer were observed. A luminance of 920 cd/m(2) and luminous efficiency of 5.35 1m/W were achieved at a drive voltage of 15 V for the multilayer device. (C) 1997 Elsevier Science S.A.
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本文用甲苯/丁酮混合溶剂对四种国产高抗冲聚苯乙烯(HIPS)进行了溶解—离心分离;测定了溶胶和凝胶含量;用透射电子显微镜研究了HIPS及其凝胶组分的形态结构;用动态力学方法测定了四个试样的转变与松弛过程。结果表明:试样1的分散相区尺寸较小,聚丁二烯与聚苯乙烯接枝率较高,因而具有较大的冲击强度。另外三个试样的橡胶分散相区尺寸较大,聚丁二烯与聚苯乙烯间接枝率较低,因而冲击强度较低。在试样1的动态力学性能谱上一40℃左右有一个相应于接枝点的分子松驰过程,而另外三个试样则检测不到这一松驰过程。
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热休克蛋白70是热休克蛋白家族中重要的成员,参与新生蛋白的折叠、转运、重折叠变性蛋白、协助降解变性蛋白和抗逆环境胁迫等功能。海带和裙带菜是浅海潮下带典型的褐藻,孔石莼和浒苔是潮间带典型的绿藻,四种大型海藻均有重要的经济价值和生态价值。随着潮汐变化固着藻类生境理化因素变化剧烈,藻类面临着严重的环境胁迫,因此研究藻类抗逆机理有着重要的意义。 本研究采用同源克隆法配合RACE-PCR,克隆了海带、孔石莼、裙带菜和浒苔HSP70基因的全序列(分别命名为LJHSP70、UPHSP70、QDHSP70和EPHSP70)。利用生物信息学方法分析了四种藻类HSP70结构特征、同源性关系和进化地位。获得的海带HSP70基因全序列长为2918 bp,5’非翻译区为248 bp,3’非翻译区为696 bp,开放阅读框为1974 bp,编码657个氨基酸,预测的分子量为72.03 kDa,等电点为4.97。获得的裙带菜HSP70基因全序列长为3243 bp,5’非翻译区为248 bp,3’非翻译区为1021 bp,开放阅读框为1974 bp,编码657个氨基酸,预测的分子量为72.03 kDa,等电点为4.96。获得的孔石莼HSP70基因全序列长为2283 bp,5’非翻译区为65 bp,3’非翻译区为247 bp,开放阅读框为1971 bp,编码656个氨基酸,预测的分子量为71.13 kDa,等电点为5.04。获得的浒苔HSP70基因全序列长为2265 bp,5’非翻译区为65 bp,3’非翻译区为217 bp,开放阅读框为1983 bp,编码660个氨基酸,预测的分子量为71.39 kDa,等电点为5.03。四种海藻HSP70氨基酸序列均含有四肽重复序列GGMP,具有三个典型的HSP70签名基序。细胞质定位的HSP70 C-末端特征基序为EEID或EEVD,并且N-端氨基酸序列保守性高于C-端。海带和裙带菜HSP70蛋白同源性为98%,孔石莼和浒苔HSP70蛋白同源性为96%,四种海藻HSP70蛋白序列与陆地植物和其他藻类HSP70蛋白序列同源性为70-80%。 利用荧光定量RT-PCR技术对不同胁迫条件处理的海带和孔石莼HSP70 mRNA的表达水平进行定量分析。不同热激温度(5-40 ℃)处理组中,30 ℃处理组的海带HSP70 mRNA表达量最高是10 ℃处理组的海带HSP70 mRNA表达量的3倍,而35 ℃或40 ℃处理组的海带HSP70表达量却低于25 ℃或30 ℃处理组的海带HSP70 mRNA表达量。25 ℃不同热激时间(0-12 h)处理组中,海带HSP70 mRNA表达量呈先上升后下降趋势。热激1 h后海带HSP70 mRNA表达量迅速上升,热激7 h后mRNA表达量达到最大,是对照组表达量的4倍。不同盐度(0‰-45‰)胁迫处理组中,0‰或5‰盐度处理组的海带HSP70 mRNA表达量是30‰盐度处理组海带HSP70 mRNA表达量的3倍。35‰、40‰和45‰盐度处理组之间HSP70 mRNA表达量较低且无显著差异。 不同热激温度(5-40℃)处理组中,20 ℃或25 ℃处理的孔石莼HSP70 mRNA表达量较低,而5 ℃、35 ℃、或40 ℃处理组的孔石莼HSP70 mRNA的表达量是25 ℃处理组孔石莼HSP70 mRNA表达量的2倍以上。30 ℃不同热激时间(0-12 h)处理组中,孔石莼HSP70 mRNA表达量也呈先上升后下降趋势。热激5 h后孔石莼HSP70 mRNA表达量达到最大,是对照组的3.5倍。不同盐度(0‰-45‰)胁迫处理组中,0‰或5‰盐度处理组的孔石莼HSP70 mRNA表达量是30‰盐度处理组孔石莼HSP70表达量的3倍。30‰、40‰和45‰盐度处理组孔石莼HSP70 mRNA表达量较低,且无显著差异。不同紫外线照射时间(0-4.0 h)和不同干燥时间(0-4.0 h)处理组中,孔石莼HSP70 mRNA表达量都在3 h后达到最高值,之后表达量维持在较高水平。 为进一步研究藻类HSP70的生物学功能,将海带HSP70基因的开放阅读框区域克隆到表达载体pEASY-E2中,并转化到大肠杆菌BL21(DE3)pLysS。将阳性重组子培养于含有AMP(100 U/mL)的LB培养基,IPTG诱导表达,SDS-PAGE电泳鉴定。经5 h诱导,其表达量达到平台期,继续培养HSP70表达量并不显著增高。5 mM IPTG诱导海带HSP70蛋白表达量高于1 mM IPTG诱导蛋白表达量。
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Grazing intensity may alter the soil respiration rate in grassland ecosystems. The objectives of our study were to (1) determine the influence of grazing intensity on temporal variations in soil respiration of an alpine meadow on the northeastern Tibetan Plateau; and (2) characterise, the temperature response of soil respiration under different grazing intensities. Diurnal and seasonal soil respiration rates were measured for two alpine meadow sites with different grazing intensities. The light grazing (LG) meadow site had a grazing intensity of 2.55 sheep ha(-1), while the grazing intensity of the heavy grazing (HG) meadow site, 5.35 sheep ha(-1), was approximately twice that of the LG site. Soil respiration measurements - showed that CO2 efflux was almost twice as great at the LG site as at the HG site during the growing season, but the diurnal and seasonal patterns of soil respiration rate were similar for the two sites. Both exhibited the highest annual soil respiration rate in mid-August and the lowest in January. Soil respiration rate was highly dependent on soil temperature. The Q(10) value for annual soil respiration was lower for the HG site (2.75) than for the LG site (3.22). Estimates of net ecosystem CO2 exchange from monthly measurements of biomass and soil respiration revealed that during the period from May 1998 to April 1999, the LG site released 2040 g CO2 m(-2) y(-1) to the atmosphere, which was about one third more than the 1530g CO2 m(-2) y(-1) released at the HG site. The results suggest that (1) grazing intensity alters not only soil respiration rate, but also the temperature dependence of soil CO2 efflux; and (2) soil temperature is the major environmental factor controlling the temporal variation of soil respiration rate in the alpine meadow ecosystem. (C) 2003 Elsevier Ltd. All fights reserved.
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Background: The telephone is an accepted and useful means of communication for the management of patient care. The Chemotherapy Telephone Helpline (CTH) service, located in a large inner-city Trust within the United Kingdom, is a unique nurse-led service within Northern Ireland.
Objective: The objective of the study was to investigate the utility, caller, and patient profile of a novel CTH.
Methods: This was a retrospective study of telephone contacts during 2007 to the CTH. Calls were categorized by caller and patient characteristics, reason for call, and subsequent action.
Results: A total of 7498 calls were made to the CTH during 2007. Of these, 25.6% occurred outside 8AM-4PM. Callers included patients (45.8%), lay carers (31%), and health care professionals (20.5%); 35.2% of calls concerned patients with polysymptomatic problems; 36.8% of calls led directly to patients being medically assessed.
Conclusions: The utility of the CTH service confirms the need of this nurse-led service. This service facilitates access to specialist advice and support for patients, their families, and allied health care professionals.
Implications for Practice: The international significance of these findings for practice includes its demonstration of the multifaceted symptom experience of patients receiving chemotherapy and highlights the importance of rapid access to specialist cancer services for patients and their lay and professional carers. In addition, it demonstrates the capacity of helplines to identify gaps in professional skills and training.
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The use of two gold compounds incorporated into thin plastic films as luminescence quenching oxygen sensors is described. The films are sensitive both to gaseous oxygen and to oxygen dissolved in nonaqueous media such as ethanol. The luminescence quenching of these sensors by oxygen obeys the Stern-Volmer equation and Stern-Volmer constants of 5.35 x 10(-3) and 0.9 x 10(-3) Torr(-1) are found, respectively, for the two dyes in a polystyrene polymer matrix. The sensitivity of the films is strongly influenced by the nature of the polymer matrix, and greatest sensitivity was found in systems based an the polymers polystyrene or cellulose acetate butyrate. Sensitivity was not found to be temperature dependent though raising the temperature hom 15 to 50 degrees C did result in a slight decrease in emission intensity and a hypsochromic shift in the emission wavelength. The rate of response and recovery of the sensors can be increased either by decreasing film thickness or by increasing the operating temperature. The operational and storage stability of these films is generally good though exposure to light should be avoided as one of the dyes tends to undergo photobleaching probably due to a photoinduced ligand substitution reaction.