Nitric oxide-induced cytostasis and cell cycle arrest of a human breast cancer cell line (MDA-MB-231): Potential role of cyclin D1

DETA-NONOate, a nitric oxide (NO) donor, induced cytostasis in the human breast cancer cells MDA-MB-231, and the cells were arrested in the G1 phase of the cell cycle. This cytostatic effect of the NO donor was associated with the down-regulation of cyclin D1 and hypophosphorylation of the retinoblastoma protein. No changes in the levels of cyclin E or the catalytic partners of these cyclins, CDK2, CDK4, or CDK6, were observed. This NO-induced cytostasis and decrease in cyclin D1 was reversible for up to 48 h of DETA-NONOate (1 mM) treatment. DETA-NONOate (1 mM) produced a steady-state concentration of 0.5 μM of NO over a 24-h period. Synchronized population of the cells exposed to DETA-NONOate remained arrested at the G1 phase of the cell cycle whereas untreated control cells progressed through the cell cycle after serum stimulation. The cells arrested at the G1 phase after exposure to the NO donor had low cyclin D1 levels compared with the control cells. The levels of cyclin E and CDK4, however, were similar to the control cells. The decline in cyclin D1 protein preceded the decrease of its mRNA. This decline of cyclin D1 was due to a decrease in its synthesis induced by the NO donor and not due to an increase in its degradation. We conclude that down-regulation of cyclin D1 protein by DETA-NONOate played an important role in the cytostasis and arrest of these tumor cells in the G1 phase of the cell cycle.

Operator's manual : tractor, crawler, low speed, D.E.D., 16,000 to 24,000 lb. drawbar pull, 60 in. min gage, segmented, air-transportable, w/mounting kit, (Caterpiller models D5A), FSN 2410-142-5283, FSN 2410-230-2767.

"March 1972."

Efectos del terreno en la propagación electromagnética en entornos urbanos sobre la región andina, usando el modelo COST 231-Walfisch-Ikegami y herramientas de planificación basadas en GIS

El presente artículo hace parte del desarrollo de un algoritmo que considera la influencia del relieve en la propagación electromagnética para un entorno semi-urbano, trabajando en la banda de UHF (300MHz-3GHz) utilizada en los actuales y futuros sistemas de comunicación inalámbricos (i.e. 800-900MHz en sistemas DAMPS/US-TDMA/IS-136 y GSM). El modelo base, el COST231-Walfisch- Ikegami, empleado en la investigación,demostró beneficios, junto con los Sistemas de Información Geográfica (SIG) y las Herramientas de Planificación, para el desarrollo de los estudios de propagación, estimación de coberturas y análisis de los principales factores que afectan la planificación de un sistema móvil celular. Aquí se describen los conceptos básicos utilizados para el desarrollo del algoritmo aplicado, las consideraciones sobre las cuales se llevaron a cabolas campañas de medidas y el proceso de validación de resultados que comprueban la utilidad del algoritmo desarrollado para la predicción de Path Loss. El trabajo se basó en la fusión del modelo de propagación COST231-Walfisch-Ikegami con laHerramienta de Planificación Cell-View (fundamentada en el SIG ArcView) y la realización de mediciones en la ciudad de Bucaramanga con una unidad móvil de radiocomunicacióny control del espectro radioeléctrico de propiedad del Ministerio de Comunicaciones de Colombia, seccional Bucaramanga. El estudio llevado a cabo para la banda del sistema IS- 136, mostró resultados de simulación y validación que permiten corroborarlas aproximaciones empleadas.

Hungry 24/7? HCI design for sustainable food culture workshop

This workshop proposes to explore new approaches to cultivate and support sustainable food culture in urban environments via human computer interaction design and ubiquitous technologies. Food is a challenging issue in urban contexts: while food consumption decisions are made many times a day, most food interaction for urbanites occurs based on convenience and habitual practices. This situation is contrasting to the fact that food is at the centre of global environment, health, and social issues that are becoming increasingly immanent and imminent. As such, it is timely and crucial to ask: what are feasible, effective, and innovative ways to improve human-food-interaction through human-computer-interaction in order to contribute to environmental, health, and social sustainability in urban environments? This workshop brings together insights across disciplines to discuss this question, and plan and promote individual, local, and global change for sustainable food culture.