Induction of topoisomerase I cleavage complexes by 1-β-d-arabinofuranosylcytosine (ara-C) in vitro and in ara-C-treated cells

1-β-d-Arabinofuranosylcytosine (Ara-C) is a nucleoside analog commonly used in the treatment of leukemias. Ara-C inhibits DNA polymerases and can be incorporated into DNA. Its mechanism of cytotoxicity is not fully understood. Using oligonucleotides and purified human topoisomerase I (top1), we found a 4- to 6-fold enhancement of top1 cleavage complexes when ara-C was incorporated at the +1 position (immediately 3′) relative to a unique top1 cleavage site. This enhancement was primarily due to a reversible inhibition of top1-mediated DNA religation. Because ara-C incorporation is known to alter base stacking and sugar puckering at the misincorporation site and at the neighboring base pairs, the observed inhibition of religation at the ara-C site suggests the importance of the alignment of the 5′-hydroxyl end for religation with the phosphate group of the top1 phosphotyrosine bond. This study also demonstrates that ara-C treatment and DNA incorporation trap top1 cleavage complexes in human leukemia cells. Finally, we report that camptothecin-resistant mouse P388/CPT45 cells with no detectable top1 are crossresistant to ara-C, which suggests that top1 poisoning is a potential mechanism for ara-C cytotoxicity.

Annex 7.1.d. Pràctica 12. Coneixement de la realitat

El document és l'annex de la pràctica 12. Coneixement de la realitat en Educació Infantil.

Regional subsidence modelling in Murcia city (SE Spain) using 1-D vertical finite element analysis and 2-D interpolation of ground surface displacements

Subsidence is a hazard that may have natural or anthropogenic origin causing important economic losses. The area of Murcia city (SE Spain) has been affected by subsidence due to groundwater overexploitation since the year 1992. The main observed historical piezometric level declines occurred in the periods 1982–1984, 1992–1995 and 2004–2008 and showed a close correlation with the temporal evolution of ground displacements. Since 2008, the pressure recovery in the aquifer has led to an uplift of the ground surface that has been detected by the extensometers. In the present work an elastic hydro-mechanical finite element code has been used to compute the subsidence time series for 24 geotechnical boreholes, prescribing the measured groundwater table evolution. The achieved results have been compared with the displacements estimated through an advanced DInSAR technique and measured by the extensometers. These spatio-temporal comparisons have showed that, in spite of the limited geomechanical data available, the model has turned out to satisfactorily reproduce the subsidence phenomenon affecting Murcia City. The model will allow the prediction of future induced deformations and the consequences of any piezometric level variation in the study area.

La mujer española en Indias : disertacion leída ante la Real Academia de la Historia en la sesión pública celebrada el día 1. d̊e junio de 1902 para la adjudicación de premios /

Includes bibliographical references.

Reglamentos y tarifas para pasageros, encomiendas, equipages, carga, telégrafos, etc., etc., 1 d̊e noviembre de 1886.

Mode of access: Internet.

Trio, no. 1, D minor, for piano, violin, and cello, Op. 49. [music] /

Title page varies.

Nouveau traité de matière médicale, de thérapeutique et de pharmacie vétérinaire, suivi 1⁰ D'un abrégé de toxicologie vétérinaire, 2⁰ De notions sur la pharmacie légale vétérinaire, 3⁰ D'un formulaire raisonné, magistral et officinal, 4⁰ D'un mémorial général de thérapeutique,

Mode of access: Internet.

Encyclopédie oeconomique ou système général : 1° d'oeconomie rustique ... 2° d'oeconomie domestique ... 3° d'oeconomie politique ...

Contiene: Tome VII. - Tome X. - Tome XI. - Tome XIII. - Tome XIV. - Tome XVI

Manuel de l'expropriation pour cause d'utilité publique : suivi, 1?, d'un commentaire sur l'expropriation en Algérie ; 2?, de la législation complète et d'un formulaire des actes en cette matière /

Mode of access: Internet.

Tercer censo nacional, levantado el 1 d̊e junio de 1914, ordenado por la Ley no. 9108 bajo la presidencia del dr. Roque Saenz Peña, ejecutado durante la presidencia del dr. Victorino de la Plaza..

Microfilm. LaCrosse, Wis., Northern Micrographics for Brookhaven Press, Washington, D.C., 1977. 3 reels. 35 mm

Concerto no. 1, (D major) for violin and piano /

Mode of access: Internet.

Violin-Konzert No. 1, D moll, Opus 7, No. 1 /

Publisher's no.: Edition Peters No. 2967a.