Forced expression of tropomyosin 2 or 3 in v-Ki-ras-transformed fibroblasts results in distinct phenotypic effects.

Transformation of cells in tissue culture results in a variety of cellular changes including alterations in cell growth, adhesiveness, motility, morphology, and organization of the cytoskeleton. Morphological and cytoskeletal changes are perhaps the most readily apparent features of transformed cells. Although a number of studies have documented a decrease in the expression of specific tropomyosin (TM) isoforms in transformed cells, it remains to be determined if the suppression of TM synthesis is essential in the establishment and maintenance of the transformed pheno-type. To address the roles of different TM isoforms in transformed cells we have examined the effects of expressing specific TM isoforms in transformed cells using a Kirsten virus-transformed cell line (ATCC NRK1569) as a model system. In contrast to normal fibroblasts, the NRK 1569 cells contain reduced levels of TM-1 and undetectable levels of TM-2 and TM-3. These cells have a rounded morphology and are devoid of stress fibers. Employing expression plasmids for TM-2 and TM-3, stable cell lines were established from the NRK 1569 cells that express these isoforms individually. We demonstrate that expression of TM-2 or TM-3 leads to increased cell spreading accompanied by the formation of identifiable microfilament bundles, as well as significant restoration of well-defined vinculin-containing focal adhesion plaques, although expression of each isoform exhibited distinct properties. In addition, cells expressing TM-2, but not TM-3, exhibited contact-inhibited cell growth and a requirement for serum.

War Department technical manual.

Description based on: TM 1-205 (Nov. 1940).

Preparation for shipment of OH-58 helicopter.

"18 July 1977."

Mating system variation among populations, individuals and within and among fruits in Bertholletia excelsa.

The aim of this study was to investigate variation in mating system among three Brazilian Amazon populations of the tree Bertholletia excelsa with different levels of anthropogenic interventions. We collected open-pollinated seeds from one natural population, remnant trees dispersed in a pasture, and trees from a plantation. Outcrossing rate not varied among the populations and indicates that all seeds were originated from outcrossing (tm=1.0). Mating among relatives was significant higher in the plantation than forest and pasture populations, probably due the fact that many trees are related in the plantation. Correlated mating was significantly higher in pasture (rp=0.47) and plantation (rp=0.51) than in the natural population (rp=0.22), suggesting that trees in natural population are pollinated by a higher number of pollen donors. The paternity correlation was significantly higher within (rp(w)=0.41) than among fruits (rp(a)=0.18), showing a higher probability to find full-sibs within than among fruits. The fixation index was generally lower in seed trees than in their seedlings, suggesting selection for heterozygous individuals from seedling to adult stages. Progeny arrays collected from the natural population had a lower proportion of pairwise full-sibs than in pasture and plantation and higher variance effective size (2.75) than trees in pasture (2.15) and plantations (2.22). Results highlight that seed collections for conservation, breeding and reforestation programs preferentially should be carried out in natural populations due low proportion highest variance effective size within progeny.

Melhoria da Prevenção do Câncer de Colo de Útero e de Mama, na ESF Clínica da Família, Macau, RN

O câncer do colo do útero e o câncer de mama são os tipos de cânceres mais comuns entre as mulheres. A prevenção dessas doenças está baseada no rastreamento da população feminina por meio da detecção precoce de lesões pré-cancerosas, no diagnós¬tico exato do grau da lesão e no tratamento.Sendo a porta de entrada para detectar oportunamente estas doenças na unidade básica de saúde. Neste trabalho desenvolvemos um projeto de intervenção junto à população feminina da Estratégia Saúde da Família Clínica da Família de Macau-RN, com o objetivo de melhorar a Atenção a Prevenção do Câncer de Colo de Útero nas mulheres de25a64 anos e Controle do Câncer de Mama nas mulheres de 50 a 69 anos de idade. Para alcançar os objetivos propostos foram estabelecidas metas e ações nos 4 eixos temáticos do curso, sendo estes organização e gestão do serviço, monitoramento e avaliação, engajamento público, e qualificação da prática clínica. O cadastro desses usuários na planilha de coleta de dados digital foi feito no momento da consulta, a qual serviu para monitoramento e acompanhamento das metas e indicadores. Para registro das atividades foi utilizado o prontuário clínico individual e a ficha-espelho. Segundo dados coletados pelo Sistema de Informação da Atenção Básica (SIAB) na área adstrita têm 1.353 mulheres na faixa de prevenção de câncer de colo de útero e 405 na faixa etária de prevenção do câncer de mama. Dessas mulheres 361 participaram da intervenção durante quatro meses e lembrando que algumas delas se incluem nas duas faixas etárias. Com a realização deste projeto conseguimos aumentar a adesão ao exame citopatológico do colo de útero, assim como as mamografias ainda que não conseguisse atingir os 100% como tínhamos previsto. Mas o total das amostras coletadas foi satisfatório atingindo 100% e as usuárias com alterações nos exames foram avaliadas pelos especialistas correspondentes. As ações multidisciplinares que foram desenvolvidas alcançaram grande importância já que sensibilizou aos profissionais de saúde em relação a realização dos exames referidos; colaborar para a realização do diagnóstico precoce do câncer do colo do útero, assim como do câncer de mama e promover ações de educação em saúde; a equipe utilizou estratégias para a realização dos exames, como: horário ampliado na unidade de saúde (dois dias da semana).Houve uma melhora significativa com respeito ao acolhimento e sistematização do atendimento. Estas ações de saúde permitiram que nossa população se conscientizasse na realização desses exames participando em todas as atividades oferecidas pela equipe e cumprindo com as orientações fornecidas.

Energy transfer processes in Yb(3+)-Tm(3+) co-doped sodium alumino-phosphate glasses with improved 1.8 mu m emission

Sodium alumino-phosphate glasses co-doped with Yb(3+) and Tm(3+) ions have been prepared with notably low OH(-) content, and characterized from the viewpoint of their spectroscopic properties. In these glasses, Yb(3+) acts as an efficient sensitizer of excitation energy at 0.98 mu m - which can be provided by high power and low cost diode lasers, and subsequently undergoes non-resonant energy transfer to Tm(3+) ions ((2)F(5/2), (3)H(6) --> (2)F(7/2), (3)H(5)). Through this process, the emitting level (3)F(4) is rapidly populated, generating improved emission at 1.8 mu m ((3)F(4) --> (3)H(6)). In order to guarantee the efficiency of such favorable energy transfer, energy losses via multiphonon decay, Yb-Yb radiative trapping, and non- radiative transfer to OH(-) groups were evaluated, and minimized when possible. The dipole - dipole energy transfer microscopic parameters corresponding to Yb(3+) --> Tm(3+), Yb(3+) --> Yb(3+) and Tm(3+) --> Tm(3+) transfers, calculated by the Forster-Dexter model, are C(Yb-Tm) = 2.9 x 10(-40) cm(6) s(-1), C(Yb-Yb) = 42 x 10(-40) cm(6) s(-1) and C(Tm-Tm) = 43 x 10(-40) cm(6) s(-1), respectively.