Effects of water potential on spore germination and viability of Fusarium species

Germination of macroconidia and/or microconidia of 24 strains of Fusarium solani, F. chlamydosporum, F. culmorum, F. equiseti, F. verticillioides, F. sambucinum, F. oxysporum and F. proliferatum isolated from fluvial channels and sea beds of the south-eastern coast of Spain, and three control strains (F. oxysporum isolated from affected cultures) was studied in distilled water in response to a range of water potentials adjusted with NaCI. (0, -13.79, -41.79, -70.37, -99.56 and -144.54 bars). The vialibility (UFC/ml) of suspension was also tested in three time periods (0,24 and 48h). Conidia always germinated in distilled water. The pattern of conidial germination obseved of F. verticillioides, F. oxysporum, F. proliferatum, F. chlamydosporum and F. culmorum was similar. A great diminution of spore germination was found in -13.79 bars solutions. Spore germination percentage for F. solani isolates was maximal at 48 h. and -13.79 bars with 21.33% spore germination, 16% higher than germination in distilled water. F. equiseti shows the maximum germination percentage in -144.54 bars solution in 24 h time with 12.36% germination. These results did not agree with those obtained in the viability test where maximum germination was found in distilled water. The viability analysis showed the great capacity of F. verticilloides strains to form viable colonies, even in such extreme conditions as -144,54 bars after 24 h F. proliferatum colony formation was prevented in the range of -70.37 bars. These results show the clear affectation of water potential to conidia germination of Fusaria. The ability of certain species of Fusarium to develop a saprophytic life in the salt water of the Mediterraneam Sea could be certain. Successful germination, even under high salty media conditions, suggests taht Fusarium spp. could have a competitive advantage over other soil fungi in crops irrigated with saline water. In the specific case of F. solani, water potential of -13.79 bars affected germination positively. It could indicate that F. solani has an special physiological mechanism of survival in low water potential environments.

Mordenite from Spain and its application as pozzolana

In Spain, natural zeolites have been only found in the volcanic areas of Canary Islands (San Gil, M.M., 1959). However, several years ago the study of the South Eastern region of continental Spain (Cabo de Gata) revealed the presence of a deposit of mordenite being industrially commercialized as bentonite. The deposit San José-Los Escullos is located in the concession registered as Los Murcianos, managed by Bentonitas Especiales, S.A. (BENESA) who was extracting bentonite while ignoring the presence of the zeolite. The name San José-Los Escullos refers to the discovery of mordenite in this deposit. The deposit is located in a volcanic area, constituted by pyroxenic andesites, breccias, tuffs, pyroclasts with andesitic and dacitic composition, largely altered by the hydrothermal solutions. The recent discovery of this zeolite with mordenite content up to 97%, has changed the initial vision about that deposit, and new possible uses of this mineral in the pozzolanic cement industry are foreseen. This work intends to give preliminary data on the characterization of this zeolite according to X-ray diffraction (XRD), ICP analyses, and scanning electron microscopy (SEM), as well as to emphasize the pozzolanic properties of this material, using both chemical and mechanical tests.

Foreword special issue Lorca’s earthquake

(This is an excerpt from the content) On May 11 2011 at 1705 hours, a small 4.5 Mw. magnitude earthquake struck the town of Lorca in south-eastern Spain. Other than alarmed citizens, only minor damage to buildings occurred due to this quake. Unfortunately at 1847 hours, a second shock registering a magnitude of 5.1 Mw. and very shallow (just around 2 km under the city) produced the largest seismic catastrophe registered in Spain in the last 120 years. This second shock is commonly referred to as “Lorca’s earthquake” and the following papers describe the context, circumstances and consequences of the event. Spain is a country of moderate seismic hazard in a global context. Before the Lorca earthquake, the most destructive earthquake in modern times was the so-called “Andalusian earthquake” (25th December 1884) that resulted in 750 fatalities and more than 1,500 injuries, reaching X in Mercalli’s intensity scale. Despite the lack of catastrophic events in the last 120 years, Spain has always had a scientific interest in seismic ...

Optimization of water procurement decisions in an irrigation district: the role of option contracts

Water supply instability is one of the main risks faced by irrigation districts and farmers. Water procurement decision optimisation is essential in order to increase supply reliability and reduce costs. Water markets, such as spot purchases or water supply option contracts, can make this decision process more flexible. We analyse the potential interest in an option contract for an irrigation district that has access to several water sources. We apply a stochastic recursive mathematical programming model to simulate the water procurement decisions of an irrigation district?s board operating in a context of water supply uncertainty in south-eastern Spain. We analyse what role different option contracts could play in securing its water supply. Results suggest that the irrigation district would be willing to accept the proposed option contract in most cases subject to realistic values of the option contract financial terms. Of nine different water sources, desalination and the option contract are the main substitutes, where the use of either depends on the contract parameters. The contract premium and optioned volume are the variables that have a greater impact on the irrigation district?s decisions. Key words: Segura Basin, stochastic recursive programming, water markets, water supply option contract, water supply risk.

EM NOME DA ORDEM : O papel dos agentes da religião católica institucionalizada durante o movimento e guerra do Contestado entre 1912-1916

Esta tese analisa o papel dos líderes da Igreja católica institucionalizada antes e durante o movimento e guerra do Contestado de 1912 a 1916. Movimento classificado como messiânico-milenarista ocorrido no sul do Brasil numa área litigiosa pleiteada pelos Estados do Paraná e Santa Catarina. Aponta o contexto sócio-econômico-político e religioso que criou condições para que o movimento pudesse emergir. Analisa a formação do catolicismo rústico popular brasileiro e suas expressões na região do Contestado, destacando-se o papel dos monges. Aprofunda a compreensão do catolicismo oficial em processo de romanização e do papel dos frades franciscanos, de suas concepções e práticas na tentativa de enquadrar a religião cabocla dentro dos princípios da reforma. Analisa a reação cabocla diante das mudanças implantadas na região e a explosão da irmandade, denominada Terra Santa , que buscava efetivar uma nova ordem . Aprofunda especialmente o papel dos frades representantes da Igreja católica, tendo em vista dispersar e pacificar o caboclo rebelde e a aliança, que estabeleceram com as forças repressoras, para implantar a ordem e a paz na região contestada, com a conseqüente eliminação radical da irmandade cabocla e de seus redutos .(AU)

EM NOME DA ORDEM : O papel dos agentes da religião católica institucionalizada durante o movimento e guerra do Contestado entre 1912-1916

Esta tese analisa o papel dos líderes da Igreja católica institucionalizada antes e durante o movimento e guerra do Contestado de 1912 a 1916. Movimento classificado como messiânico-milenarista ocorrido no sul do Brasil numa área litigiosa pleiteada pelos Estados do Paraná e Santa Catarina. Aponta o contexto sócio-econômico-político e religioso que criou condições para que o movimento pudesse emergir. Analisa a formação do catolicismo rústico popular brasileiro e suas expressões na região do Contestado, destacando-se o papel dos monges. Aprofunda a compreensão do catolicismo oficial em processo de romanização e do papel dos frades franciscanos, de suas concepções e práticas na tentativa de enquadrar a religião cabocla dentro dos princípios da reforma. Analisa a reação cabocla diante das mudanças implantadas na região e a explosão da irmandade, denominada Terra Santa , que buscava efetivar uma nova ordem . Aprofunda especialmente o papel dos frades representantes da Igreja católica, tendo em vista dispersar e pacificar o caboclo rebelde e a aliança, que estabeleceram com as forças repressoras, para implantar a ordem e a paz na região contestada, com a conseqüente eliminação radical da irmandade cabocla e de seus redutos .(AU)

Fish diversity and traditional fishing methods in semiarid irrigated system in the Bajo Vinalopó region, Southeastern Spain

The aim of this paper is to obtain a comprehensive picture of the importance of the fish fauna in the wetlands and coastal lagoons of the Bajo Vinalopó region (Alicante, South eastern Spain) and the ecological, historical and cultural value of the traditional knowledge on fishing maintained by local people. We have compiled data from different information sources: (1) the revision of historical archives, (2) personal interviews and collaborative research, and (3) ecological and water quality data. The results show that the area sustains important fish fauna diversity (17 species) and that local people have inherited a considerable traditional knowledge on fishing methods (11 modalities), on the maintenance and sound management of the water system and on the ecology and behaviour of fish. We conclude that a comprehensive consideration of all these ecological, historical and socio-cultural aspects related to fish and fishing shows clearly the value of this ecological and cultural heritage and provides a necessary base for a sustainable management of the area.

Effects of landscape spatial heterogeneity on dryland restoration success. The combined role of site conditions and reforestation techniques in southeastern Spain

In occidental Europe, Spain is one of countries the most severely affected by desertification (Arnalds & Arsher 2000). Particularly, South-eastern Spain is considered as one of the most threatened areas by desertification in Mediterranean Europe (Vallejo 1997). In 2003, the Valencia Regional Forest Service implemented a restoration demonstration project in this area. The project site is a small catchment (25 ha) located in the Albatera municipality. The catchment is highly heterogeneous, with terraced slopes, south-facing slopes and north-facing slopes. The restoration strategy was based on planting evergreen trees and shrubs which can grow quickly after disturbances, and on field treatments aimed at maximizing water collection (micro-catchments, planting furrows), organic amendment (compost), and conservation (tree shelters, mulching). On south landscape unit, the whole category of restoration treatments was applied: water micro-catchment + Tubex tree shelters + mulching & compost, while on north landscape unit: netting tree shelters + mulching & compost only were applied, while in terrace landscape unit: furrows + netting tree shelters + mulching & compost were applied. Survival and growth of the planted seedlings were used as metrics of restoration success. To assess the effects of the treatments applied for soil conservation, soil loss rates (from 2005 to 2009) were evaluated using the erosion pin method. We conclude that, despite the limiting conditions prevailing on the south unit, this landscape unit showed the highest survival and growth plant rates in the area. The best seedling performances on the south landscape unit were probably due to the highest technical efforts applied, consisting in the water micro-catchment installation and the Tubex plant shelters addition. In addition, soil loss rates followed decreasing trends throughout the assessment period. Soil loss rates were highest on south landscape unit in comparison with the other landscape units, due to the more accentuated relief. North landscape unit and terrace unit showed a net soil mass gain, probably reflecting the trapping of sediments produced by plantation works.

A PCR based method to detect Russula spp. in soil samples and Limodorum abortivum roots in Mediterranean environments

Aim of study. Orchidaceae has the largest number of species of any family in the plant kingdom. This family is subject to a high risk of extinction in natural environments, such as natural parks and protected areas. Recent studies have shown the prevalence of many species of orchids to be linked to fungal soil diversity, due to their myco-heterotrophic behaviour. Plant communities determine fungal soil diversity, and both generate optimal conditions for orchid development. Area of study. The work was carried out in n the two most important natural parks in Alicante (Font Roja and Sierra Mariola), in South-eastern of Spain. Material and Methods. We designed a molecular tool to monitor the presence of Russula spp. in soil and orchids roots, combined with phytosociological methods. Main results. Using a PCR-based method, we detected the presence in the soil and Limodorum abortivum orchid roots of the mycorrhizal fungi Russula spp. The species with highest coverage was Quercus rotundifolia in areas where the orchid was present. Research highlights. We present a useful tool based on PCR to detect the presence of Russula spp. in a natural environment. These results are consistent with those obtained in different studies that linked the presence of the mycorrhizal fungi Russula spp. in roots of the species Limodorum and the interaction between these fungal species and Quercus ilex trees in Mediterranean forest environments.

Missing Rings in Pinus halepensis – The Missing Link to Relate the Tree-Ring Record to Extreme Climatic Events

Climate predictions for the Mediterranean Basin include increased temperatures, decreased precipitation, and increased frequency of extreme climatic events (ECE). These conditions are associated with decreased tree growth and increased vulnerability to pests and diseases. The anatomy of tree rings responds to these environmental conditions. Quantitatively, the width of a tree ring is largely determined by the rate and duration of cell division by the vascular cambium. In the Mediterranean climate, this division may occur throughout almost the entire year. Alternatively, cell division may cease during relatively cool and dry winters, only to resume in the same calendar year with milder temperatures and increased availability of water. Under particularly adverse conditions, no xylem may be produced in parts of the stem, resulting in a missing ring (MR). A dendrochronological network of Pinus halepensis was used to determine the relationship of MR to ECE. The network consisted of 113 sites, 1,509 trees, 2,593 cores, and 225,428 tree rings throughout the distribution range of the species. A total of 4,150 MR were identified. Binomial logistic regression analysis determined that MR frequency increased with increased cambial age. Spatial analysis indicated that the geographic areas of south-eastern Spain and northern Algeria contained the greatest frequency of MR. Dendroclimatic regression analysis indicated a non-linear relationship of MR to total monthly precipitation and mean temperature. MR are strongly associated with the combination of monthly mean temperature from previous October till current February and total precipitation from previous September till current May. They are likely to occur with total precipitation lower than 50 mm and temperatures higher than 5°C. This conclusion is global and can be applied to every site across the distribution area. Rather than simply being a complication for dendrochronology, MR formation is a fundamental response of trees to adverse environmental conditions. The demonstrated relationship of MR formation to ECE across this dendrochronological network in the Mediterranean basin shows the potential of MR analysis to reconstruct the history of past climatic extremes and to predict future forest dynamics in a changing climate.

Thessaloniki ten years on: Injecting momentum into the enlargement process for the Western Balkans. CEPS Commentary, 16 May 2013.

Ten years have passed since the EU-Western Balkans summit was held in Thessaloniki in June 2003. In this new CEPS Commentary, CEPS Associate Senior Fellow Erwan Fouéré exhorts the EU to mark the occasion by reaffirming with greater determination the European perspective for the countries of the region in the hope of keeping the Balkan ghosts at bay.

The Theory of Interhybridity: Socio-political Dimensions and Migration Experiences of Post-communist Western Balkan States

The Western Balkans integration within the EU has started a legal process which is the rejection of former communist legal/political approaches and the transformation of former communist institutions. Indeed, the EU agenda has brought vertical/horizontal integration and Europeanization of national institutions (i.e. shifting power to the EU institutions and international authorities). At this point, it is very crucial to emphasize the fact that the Western Balkans as a whole region has currently an image that includes characteristics of both the Soviet socialism and the European democracy. The EU foreign policies and enlargement strategy for Western Balkans have significant effects on four core factors (i.e. Schengen visa regulations, remittances, asylum and migration as an aggregate process). The convergence/divergence of EU member states’ priorities for migration policies regulate and even shape directly the migration dynamics in migrant sender countries. From this standpoint, the research explores how main migration factors are influenced by political and judicial factors such as; rule of law and democracy score, the economic liberation score, political and human rights, civil society score and citizenship rights in Western Balkan countries. The proposal of interhybridity explores how the hybridization of state and non-state actors within home and host countries can solve labor migration-related problems. The economical and sociopolitical labor-migration model of Basu (2009) is overlapping with the multidimensional empirical framework of interhybridity. Indisputably, hybrid model (i.e. collaboration state and non-state actors) has a catalyst role in terms of balancing social problems and civil society needs. Paradigmatically, it is better to perceive the hybrid model as a combination of communicative and strategic action that means the reciprocal recognition within the model is precondition for significant functionality. This will shape social and industrial relations with moral meanings of communication.

Bilateral Disputes – A dark cloud over the Balkans. CEPS Commentary, 24 January 2014

Countless bilateral disputes continue to plague the Balkan region; daily reminders of the region's troubled history. Not only are they the cause of tension between the countries of the region both inside and outside the EU, they also undermine efforts to integrate the region into the EU. To avoid the risk of instability and further erosion of the credibility of the EU's enlargement strategy, urgent action is required. The author sets out four recommendations for the EU to pursue without delay.

How should the EU respond to Russia's war in Ukraine? CEPS Commentary, 3 September 2014

President Putin has chosen to escalate the conflict in south-eastern Ukraine, thereby ignoring both the sanctions and the diplomatic advances of the EU and its partners in response to Russia’s belligerence towards its neighbour over the last six months. Ahead of the NATO Summit in Wales on September 4-5, calls are growing for EU member states to provide arms and more intelligence to Ukraine’s beleaguered army. But it is far from certain that the EU will summon up the collective courage to do so. Beyond sanctions, what other options does the EU have to alter the Kremlin’s calculus?

EU integration and party politics in the Balkans. EPC Issue Paper No.77, September 2014

Throughout the Balkan region, popularly elected leaders consistently fail to meet the democratic standards set by the EU and, more importantly, they fall short of their voters’ expectations. Distrust in representative institutions and disengagement from political life runs dramatically high among the people of the Balkan countries, and this generalised sense of dissatisfaction is starting to breed cynicism also towards the idea of a better future inside the Union. This study highlights the interplay between EU integration and national politics in the region, and that both are consequential for the quality of Balkan democracies, as well as reminiscent of the Western and Central and Eastern European experience.