Variation of genetic expression during development, revealed by esterase patterns in Aedes aegypti (Diptera, Culicidae)

Polyacrylamide gel electrophoresis was used to analyze esterase patterns during development of Aedes aegypti from the cities of Marília and São José do Rio Preto (SJRP), Brazil. The zymograms showed a total of 23 esterase bands, 22 of which were in the specimens from Marília and 19 in those from SJRP. These esterase bands were considered to be the product of 23 alleles distributed tentatively in eight genetic loci. Most of the alleles were developmentally regulated. The larval stage expressed the greatest number of them (19 alleles, from the eight loci, in Marília; and 17 alleles, from seven loci, in SJRP). The pupal stage expressed 10 alleles from seven loci, in both populations, and the adult stage expressed 8 alleles from five and six loci in SJRP and Marília, respectively. Some alleles that were active in every stage were developmentally controlled at the level of expression (amount of product). A single allele was constitutively and highly expressed, in larvae, pupae, and adults, in both populations. Differences in esterase synthesis among stages are probably due to regulatory mechanisms acting in agreement with the requirements of a variable number of processes in which esterases are involved. The larval stage is the most active in developmental processes and shows very intense intake of food and very high mobility. These features may demand increased esterase production at that stage. Comparison of the two populations examined showed (besides the existence of alleles that they do not share) that they exhibit differences in the control of expression of other alleles. Such findings may reflect genetic differences between founders in each population, but the possibility of involvement of the intensive use of insecticides in SJRP is also discussed.

Minimally processed guava Fruits (Psidium guaiava L.)

'Paluma' guavas, after internal quality evaluation using magnetic resonance tomography, were used to produce fresh-cut product. Fruits were peeled or not, cut in halves and seed removed, and they were packaged in polystyrene trays covered with PVC film or in a PET container with a lid. These packages were stored for 12 days at 5°C, 10°C and ambient temperature (22.6°C). Tomography evaluation verified that impacts produced internal bruising with loss of cellular integrity and liquefication of the placenta tissues. Compression was more evident on the pericarp and cutting promoted superficial deformation. Storage temperature affected the weight loss, with fruit packaged in the polystyrene tray having a greater weight loss. The peeling did not influence weight loss. Product stored at 5°C and 10°C for 8 days had low microbial growth (<103 UFC.g-1) and no coliforms. Rapid spoilage and a short shelf life (3-4 days) occurred when the product was stored at ambient temperature. Peeling reduced ascorbic acid concentration and total soluble solids. Use of calcium to protect fresh-cut products was not efficient. Calcium absorption capacity of 'Pedro Sato' guava was tested using 45Ca. Fruits treated with 2% CaCl2, with or without the radioisotope, were divided in four layers (epicarp, mesocarp, endocarp and seed) and analyzed for the total and 45Ca calcium. It was observed that the applied calcium remained in superficial layers of45fruits, which was confirmed by autoradiography. Internal layers did not contain 45Ca, indicating that calcium was not distributed into different parts of the fruit.

Diferenças regionais do cultivo de café no Brasil

Brazil is one of the major coffee producers in the world, because of this, the goal of this study was to assess the regional differences of coffee cultivation for the reference crops 2001/2002 and 2002/03 by means of a life cycle assessment (LCA) in order to generate detailed production inventory data as well as quantify the potential environmental impacts of this crop. All information considered in this study (use of water, fossil based energy, fertilizers, pesticides and correctives) was taken from data collected from the producing farms. Four Brazilian coffee producer regions located at the Southeastern region were evaluated: Sul de Minas Gerais and Cerrado Mineiro in Minas Gerais State, and Mogiana and Alta Paulista regions in São Paulo State. The data refer to a production of 25.2 million kg of green coffee. Depending on the considered region, the production of 1,000 kg of green coffee requires, on average, approx. 9,300 to 13,000 kg of total energy, 70 to 130 kg of diesel, 6,500 to 12,700 kg of process water, 270 to 340 kg of fertilizers (NPK), 2.0 to 13.0 kg of pesticides, 230 to 600 kg of correctives, and yield around 1,600 to 1,900 kg/ha. Despite 20% of the coffee growers showing a good environmental performance, i.e. consumption of pesticides, fertilizers and correctives lower than the regional averages, this study has also identified some farms that can probably reduce the amount of some inputs and enhance their environmental performance.

Technological changes and industrial dynamics in Latin America

Includes bibliography

Sustainable production of biologically active molecules of marine based origin

The marine environment offers both economic and scientific potential which are relatively untapped from a biotechnological point of view. These environments whilst harsh are ironically fragile and dependent on a harmonious life form balance. Exploitation of natural resources by exhaustive wild harvesting has obvious negative environmental consequences. From a European industry perspective marine organisms are a largely underutilised resource. This is not due to lack of interest but due to a lack of choice the industry faces for cost competitive, sustainable and environmentally conscientious product alternatives. Knowledge of the biotechnological potential of marine organisms together with the development of sustainable systems for their cultivation, processing and utilisation are essential. In 2010, the European Commission recognised this need and funded a collaborative RTD/SME project under the Framework 7-Knowledge Based Bio-Economy (KBBE) Theme 2 Programme 'Sustainable culture of marine microorganisms, algae and/or invertebrates for high value added products'. The scope of that project entitled 'Sustainable Production of Biologically Active Molecules of Marine Based Origin' (BAMMBO) is outlined. Although the Union is a global leader in many technologies, it faces increasing competition from traditional rivals and emerging economies alike and must therefore improve its innovation performance. For this reason innovation is placed at the heart of a European Horizon 2020 Strategy wherein the challenge is to connect economic performance to eco performance. This article provides a synopsis of the research activities of the BAMMBO project as they fit within the wider scope of sustainable environmentally conscientious marine resource exploitation for high-value biomolecules. © 2013 Elsevier B.V.

A moda contemporânea e a sustentabilidade no jeanswear: estudos de caso

Pós-graduação em Design - FAAC

Efeito de produtos a base de Bacillus thuringiensis Berliner em aspectos biológicos de Podisus nigrispinus (Dallas, 1851) (Hemiptera: Pentatomidae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliações dos tecidos hidrorrepelentes de equipamentos de proteção individual após usos e lavagens em condições de campo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise dos impactos na construção civil: avaliação do ciclo de vida em chapas de partículas para forros

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Avaliação do estado nutricional de gestantes do município de Botucatu, quanto a macro e micro nutrientes em relação à renda no período de 1996-1998

Pós-graduação em Fisiopatologia em Clínica Médica - FMB

Agentes públicos e privados na refuncionalização de formas urbanas na cidade de Campinas - SP

Pós-graduação em Geografia - IGCE

Estudo químico e biológico do fungo endofítico Phomopsis sp. isolado da Senna spectabilis

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

The tourism sector and the global economic crisis: development implicatins for the Caribbean

Over the past two years the global economy has experienced substantial economic turmoil, resulting in severe economic contraction. While there has been a recent return to growth, this situation has impacted all economic sectors worldwide. In the highly tourism-dependent region of the Caribbean, the impact of the global economic crisis has been most notable on the tourism sector, which, from the early 1990s, became the key driver of economic growth for the region. The eventual emergence of this sector reflects an economic development history which was previously underpinned by the export of agricultural commodities, and subsequently by the adoption of the import substitution industrialization model as promulgated by Arthur Lewis. This was further stimulated by spectacular economic contraction in Caribbean economies during the 1980s as a result of changes in the global terms of trade for commodities, generally low levels of competitiveness for manufactured goods, as well as weak institutional and governance frameworks. Ultimately, many economies began to reflect fiscal and balance of payments constraints. By the end of the 1990s, too, evidence of declining competitiveness even in the tourism sector began to become apparent particularly when evaluated under the framework of the Butler Tourism Area Life- Cycle (TALC) model. The recent economic crisis, therefore, provides an opportunity to reflect on the overall approach to economic development in the Caribbean, and to assess the implications of the region’s response to the crisis. This analysis makes the case for the future development of the sector to be based on two broad strategies. The first is to deepen the integration of the tourism sector into the broader economy through the diversification of the regional tourism product, as well as the enhancement of linkages with other sectors, while the second is to expand the tourism sector into a total service economy through the introduction of new services. Considering linkages, the development of clusters and value chains to support the tourism sector is identified with respect to agriculture and food, handicraft, and furnishings. Among the new services identified are education, wellness, yachting and boating, financial services, and information and communications technologies (ICT). This overall strategy is deemed to be better suited to the macroeconomic realities of the Caribbean, where high labour costs and other structural rigidities require a high-valued specialty tourism product in order to sustain the sector’s global competitiveness.

Efeito do óleo de nim (Azadirachta indica A. Juss) no intestino médio de Ceraeochrysa claveri (Navás, 1911) (Neuroptera: Chrysopidae): estudo citoquímico, imunocitoquímico e ultraestrutural

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Crescimento econômico na fronteira e dinâmica urbana na Amazônia: uma abordagem histórica

O objetivo deste artigo é traçar uma análise histórica dos efeitos de inserção da Amazônia paraense no contexto internacional e nacional, por intermédio das ações das políticas de articulação internacional e de integração nacional sobre a formação socioespacial do estado do Pará, principalmente, dando destaque ao processo de urbanização. A periodização adotada para descrever e analisar o processo de ocupação do espaço-território da economia paraense não segue o critério dos tradicionais ciclos de produtos, tais como o ciclo das drogas do sertão, borracha, pecuária e mineração, mas sim o critério da identificação das principais características dos padrões de ocupação e desenvolvimento econômico no espaço socioeconômico, que envolve determinações à formação do mercado nacional e do mercado internacional, com a mediação do Estado.