998 resultados para padronização de variáveis
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Introduction: The stair-climbing test (SCT) is considered a submaximal test of simple implementation and easy access, which assesses the individual’s functional capacity. Although widely used in surgical patients, there is no standardization regarding the height of the stair and verbal stimulus. Objectives: It was determine if verbal stimulus changes the stair-climbing time (SCt) in individuals over 50 years-old. We compared oxygenation, blood pressure, pulse, respiratory rate and Borg scale between SCT performed with and without stimulation. Methods: We evaluated individuals with ages greater than 50 years-old that performed two STC (with and without verbal stimulation), in the stair with 44 steps, achieving 7.04 m in height and recording the time to climb the stair. The systolic blood pressure (SBP) and the diastolic blood pressure (DBP), respiratory rate (RR), pulse, oxygen saturation (SpO2 ) and Borg scale were evaluated before and after the tests. The time in the SCT with and without stimulation were compared using the Student test-t and the other variables were compared using the ANOVA test (p<0.05). Results: The average age was 59.75±6,40 years old in the 21 evaluated individuals. The time in the SCT without stimulus was significantly higher than the one with stimulus. The SBP, pulse, RR and Borg scale were significantly increased when compared to the SCT with and without stimulus. Oxygenation and DBP didn´t have significant differences at any time of the study. Conclusions: The time in the SCT was lower when performed with verbal stimulus. The SBP, pulse, RR and Borg scale changed significantly after the SCT with and without stimulus, remarking that this change was greater in the SCT with stimulus.
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Examine the effects Global Postural Reeducation, (GPR) in hyperkyphosis and respiratory variables in the elderly was the objective in the research. For this, two elderly participated, one is 62 years old (voluntary 1) and another is 66 years old (voluntary 2), without lung, heart, kidney and/or skeletal-muscle diseases diagnosed. The volunteer have been assessed for Quality of Life (QOL) through the questionnaire SF-36, the degree of toracic kyphosis, the muscular respiratory strength and the thoracic-abdominal mobility. The GRP treatment consisted in eight sessions applied, once one hour each session. After the eight sessions the volunteer were reevaluated. The data concerning assessments before and after treatment were analysed describly. According to the results there was an improvement in the degree of kyphosis in both volunteers, highlighting voluntary 2. Respiratory variables also improvements after treatment. In relation to the Quality of life in most areas there has been an increase in scores indicating improved QOL. These data showed that the GPR has been effective to decrease the level of kyphosis, improves respiratory variables and the quality of life in the elderly treated. However, further work with a greater number of subjects must be carried out to analyze the effectiveness of therapy in the elderly.
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The effort test can be used to assess functional capacity, clinical hemodynamic and metabolic response at the effort, the risk of postoperative pulmonary complications and to assess the response of patients with lung diseases submitted to physiotherapy treatment. Among the stress tests we highlight the Six Minute Test Walk (6 MWT) and Stair Climbing Test (SCT), because they are easy to use and low cost. Especially the SCT is widely used in patients preoperatively. Objective: To compare the effects of six minute walk test and stair climbing test under the hemodynamic and oxygenation in healthy adults. Methods: We conducted a study with healthy subjects above 50 years. The fi rst performed was 6 MWT in quick step with encouragement, in a plan corridor of 30 meters, where the shade was determined the distance walked in 6 minutes, after 6 MWT was performed de SCT with encouragement, on a ladder in shade, consists of 44 steps, with 4 bids and bid by 11 steps, each step measured 16 cm in a total of 7.04 m of height, where the rise time was clocked. Before and after the two tests were measured respiratory rate, pulse, blood pressure and oxygen saturation. Results: We evaluated 21 patients with age 59.6 ± 5.4 years, 5 men and 16 women. The average distance covered on the 6MWT was 496.4 ± 102.2 meters and the average time in SCT 22.6 ± 5.4 seconds. The variables pulse, respiratory rate, systolic blood pressure and Borg scale presented a signifi - cant increase after the tests, however the variables oxygen saturation and diastolic blood pressure did not change signifi cantly. Conclusion: The variables pulse, respiratory rate, systolic blood pressure and Borg scale tests increased after six-minute walk test and stair climbing test but with greater signifi cance after the stair climbing test. Oxygen saturation and diastolic blood pressure did not change signifi cantly after the tests.
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P.brasiliensis é o agente etiológico da Paracoccidioidomicose, micose sistêmica mais importante na América Latina. A fase saprofítica produtora dos propágulos infectantes e outros aspectos ecológicos deste patógeno são ainda pouco conhecidos, o que impede a adoção de medidas preventivas. Diversas evidências indicam que o fungo se desenvolve no solo, porém o seu isolamento a partir destes materiais é raro e apenas eventual. O presente projeto visou desenvolver uma metodologia para obtenção de amostras ambientais na forma de aerossóis em locais onde a ocorrência do fungo já foi evidenciada pelo seu isolamento em tatus Dasypus novemcinctus e avaliar a ocorrência do patógeno nestes materiais por cultura direta e por biologia molecular em reações de PCR com “primers” específicos, já desenvolvidos em nosso laboratório. Para tal, avaliamos o padrão de crescimento do P. brasiliensis em quatro diferentes meios ditos como seletivos. Entre estes meios, encontra-se o Meio Sólido de amônia que, por nunca ter sido testado em P. brasiliensis, necessitava de testes quanto à viabilidade de uso e ajuste de concentração. Depois de testado a funcionalidade dos meios e observado o padrão de crescimento que o fungo exibe nesses diferentes meios, partimos para a tentativa de isolamento ambiental do P. brasiliensis através de amostras de solo em forma de aerossóis com o auxílio deles. Tendo em vista os resultados obtidos, optamos, também, por realizar a coleta de teias de aranhas, devido a grande aderência que exerce sobre aerossóis ambientais. Os isolados de P. brasiliensis cultivados apresentaram desenvolvimento semelhante nos quatro meios utilizados, indicando a possibilidade de uso destes meios seletivos para estudos ambientais. P.brasiliensis não foi isolado nestas amostras ambientais... (Resumo completo, clicar acesso eletrônico abaixo)
Acidentes ofídicos por Bothrops jararaca no Estado de São Paulo: influências de variáveis biológicas
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No Brasil ocorrem aproximadamente 20.000 acidentes ofídicos anuais, sendo um grande problema de Saúde Pública. As serpentes do gênero Bothrops são responsáveis por aproximadamente 90% dos casos, destacando-se a espécie Bothrops jararaca, responsável por aproximadamente 93% dentre os acidentes botrópicos. O presente trabalho tem como objetivo caracterizar o perfil epidemiológico dos acidentes causados por B. jararaca e avaliar possíveis influências das variáveis biológicas encontradas nos exemplares que causaram acidentes no Estado de São Paulo. Para realização desse estudo foram tomadas como base espécimes de Bothrops jararaca que causaram acidentes nos períodos de 1959 a 2011 preservadas na Coleção Vital Brasil do Instituto Butantan. Essas serpentes (N=1526) foram dissecadas e analisadas. Dados relativos à epidemiologia dos acidentes, período de atividade sazonal, processos reprodutivos e dieta foram coletados. O levantamento e análise dos dados dos acidentes mostraram que 71% dos acidentes são causados por filhotes, enquanto apenas 29% são causados por adultos. Análises preliminares mostraram que fêmeas causaram mais acidentes do que machos independente de serem filhotes ou adultos. O padrão sazonal dos acidentes foi diferente entre os filhotes e adultos de B. jararaca, o que deve estar relacionado às atividades de forrageamento, termorregulação e principalmente aos eventos reprodutivos. O perfil epidemiológico dos acidentados apontou uma maior incidência de acidentes em situações de trabalhos rurais, no caso, a maioria dos acidentados são homens jovens que foram atingidos nas mãos durante o dia
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The industries face increasingly fierce competition, so that differentiation becomes more and more difficult. In the case of the branch of machine tool industries this scenario is aggressive, those firms face competition from around the world, which have good quality and low price. This paper aims to propose the creation of a department to promote standardization in a company of special tools. The goal was achieved from survey of theories and applications of quality management, knowledge management and standardization, done by scholars in these areas, which added the use of qualitative study resulted in a proposal that contained not only the creation of this department as possible approaches that can be applied by him. This work is limited to the study of a company that manufactures special tools of the state of São Paulo, which despite its small size and limited number of employees, also presents common situations to other companies in the field, especially with regard to the behavior employees, and present good practices that can be implemented by any company
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La mission de ce rapport se trouvée dans un projet major de mise en place de la TPM dans l’usine de Biotechnologie à Huningue. Le même sera utilisé comme pilote pour les autres sites de l’entreprise. Le but spécifique de ma mission au sein de Novartis, était d’identifier les activités principales des équipes de maintenance, étudier la façon de travailler et la manière comment ils étaient renseignées dans leurs Ordres de Travail (OT). Ensuite, proposer un standard de flux de travail et façon de renseigner les OT et ainsi organiser la façon de travailler et pouvoir planifier les activités de maintenance, ayant une bonne base de données pour faire les indicateurs
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Six species of the genus Callithrix can be found across the Atlantic Forest extension, being the Callithrix geoffroyi, Geoffroy`s marmoset, the least studied species. The objective of this study is to investigate the behavioral aspects of the species in captivity, and how the environmental enrichment techniques influence those behaviors. To this end, we have studied three specimens of C. geoffroyi, in captivity at Municipal Zoo of Piracicaba, São Paulo. The enclosure includes a 37m2 with under masonry, concrete platforms with external deep, trunks, a floor of grass and soil, e grid in their other three sides. The sampling method was focal per time interval, with records every 30 seconds in sessions of 30 minutes, totaling 30 hours for each animal, which were distributed in a baseline phase, an environmental enrichment phase and post-enrichment phase (n = 3660 records in each phase). The twelve enrichment techniques were applied: the physical environment (branches for perches and hoses fabrics for bedding and vanishing points), cognitive (radio, mirror, puzzle food) and food (insects, flowers and eggs hidden, wrapped). Initially, to determine the period of greatest activity of marmosets, we investigated the pattern of activity for three days (9:00 am to 17:00 pm) consecutively at intervals of 15 minutes, recording the number of active and inactive individuals. The 495 records signaled greater activity in the morning, then selected period for the completion of this work. The ethogram comprised 16 behaviors. The inactivity was the predominant phase behavior baseline and post-enrichment with a significant decrease (h=8,62, p <0.01) in the enrichment phase, and a significant increase in the post enrichment (h=18,15, p <0.001). The same was seen for the use of substrate (grid and ground vs. Trunk and concrete, h=5,09, p <0.001 and h=3,98, p <0.05)... (Complete abstract click electronic access below)
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It is believed that epigenetic mechanisms such as DNA methylation are important for the tumorigenesis and maintenance of the altered state of tumor cells. DNA methylation occurs by the addition of a methyl group to carbon 5 of cytosine, catalyzed by the enzyme DNA methyl-transferase, which can change the expression of a gene, including the tumor suppressor genes. In human squamous cell carcinoma, several features have shown the etiological role of genes in tumor development. Among them, FOXE1 gene (forkhead box E1 - thyroid transcription factor) is presented with an important role in susceptibility to disease. Similarly the FOXE1 methylation pattern could alter the expression of this gene in dogs and predisposed to tumor on. Therefore, this study aims to investigate in dogs, the validity of the strategy employed in humans to analyze the FOXE1 methylation status. DNA extraction from fresh frozen tumoral samples was performed by Wizard Genomic® DNA Purification Kit. The methylation status was determined by MSP-PCR (methylation-specific polymerase chain reaction), using 2.0 ng of DNA treated with sodium bisulphate. One hundred micrograms of bisulphite-modified DNA was amplified using primers specific for either methylated or unmethylated DNA (primers sequences are available at http://pathology2.jhu.edu/pancreas/primer.pdf). The analysis of fragments was loaded on to 7% polyacrylamide gels and silver nitrate staining. In this stage of technical approach, 60% were FOXE1 hypermethylated. In conclusion, it was observed that the standard technique for assessing the methylation pattern of gene FOXE1 in humans can be used for the same evaluation in dogs. The correlation of these molecular data with clinical and histopathological parameters may have diagnostic and prognostic value and still be used as a tumor marker for therapeutic decision and surgical approach
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Muscle fatigue is described as a cause of injuries among the many related to the running practice. Therefore, the purpose of this study was to analyze the behavior of the amplitude (RMS) and median frequency (MF) of EMG signal of the iliocostalis (CI), rectus femoris (RF), vastus lateralis (VL), vastus medialis (VM) biceps femoris (long head) (BFCL), tibialis anterior (TA) and gastrocnemius (lateral) (LNG) from the right lower limb, and the behavior of the parameters of amplitude (AP) and frequency (PF) spent in different percentages of the maximum speed during incremental protocol of treadmill running. 10 volunteers participated in this study, athletes, male, aged between 18 and 30 years with no history of injury in lower limbs and similar anthropometry. The protocol consisted of a treadmill test with initial velocity of 10 km.h-¹ and increments of 1 km.h-¹ each three minutes until volitional exhaustion, without rest interval. Synchronized collections were made of electromyographic and kinematic data. The signals were obtained through an acquisition module of biological signals (Telemyo 900 - Noraxon - USA) and software (Myoresearch - Noraxon - USA) calibrated with a sampling frequency of 1000 Hz, gain 2000 times. The raw data were filtered with a 60Hz notch filter, high pass and low pass 20Hz to 500Hz. To capture the image was used a digital video recorder (model NV-GS320, PANASONIC brand), and for image scanning and kinematic data collection was used the software Peak Motus 9.0 (ViconPeak). To obtain the values of RMS and FM analyzed the last ten passes of each speed through a specific routine (Matlab). To obtain these variables AP (m) and FP (stride I min) were analyzed for the last ten past each speed, using specific software (Peak Motus 9.0). After verification of data normality (Shapiro-Wilk) and homogeneity of the data (Levene), the comparison ...(Complete abstract click electronic access below)
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The Atlantic forest has a large number of endemic species due to the varieties of environments, altitudes, and climates found along its distribution. The species Brachycephalus ephippium is an example of endemic anuran from this forest formation, occurring in the states of São Paulo, Rio de Janeiro and Minas Gerais, between 750 and 1200 m altitude. This species is abundant in the Serra do Japi, an Atlantic Forest remnant, which houses high biodiversity, located in Jundiaí city, between three big urban centers in the state of São Paulo. This remnant, which has altitude ranging between 700 and 1300 meters, is threatened by global climate changes and, spite of legally protected, by intense pressure of urbanization. The aim of the present study was to verify the association between of environmental variables with the abundance and distribution of B. ephippium in the Serra do Japi. The air relative humidity showed a positive correlation with the presence of B. ephippium individuals and the best model to explain the abundance involved air relative temperature and litter temperature. These results allowed the investigation of the environment use by this species and can be used to provide conservationist actions
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O contexto esportivo é repleto de muitas manifestações psicológicas. Para se ter sucesso é necessário que ocorra uma grande preparação destes atletas, que devem estar preparados fisicamente, tecnicamente e taticamente para responder às necessidades do esporte, buscando sempre a vitória e a melhor performance nas competições desportivas. A Psicologia do Esporte vem desenvolvendo programas de treinamento psicológico envolvendo técnicos, treinadores e atletas na busca de melhor desempenho nas competições. Hoje em dia o voleibol é um dos esportes mais praticados e assistidos no mundo inteiro, sendo no Brasil o segundo esporte mais praticado. O estado emocional se altera tanto em um período pré-competitivo, onde a incerteza de futuros acontecimentos provoca uma ansiedade, tensão e certo desconforto, quanto no momento da competição e também no período póscompetitivo. O sentimento de vergonha também é manifestado em muitas ocasiões. Várias são as definições encontradas para essa emoção: sentimento penoso de desonra; humilhação; rebaixamento; sentimento de insegurança provocado pelo medo do ridículo; embaraço; indignidade; timidez; acanhamento; sentimento de desconforto, entre outras. . A pesquisa buscou analisar teórica e praticamente as alterações emocionais de atletas em suas funções esportivas. Foi realizada com uma equipe de voleibol feminino, onde se aplicou um questionário fechado, a 21 atletas, com idades entre 13 e 16 anos. Verificou-se com que freqüência as manifestações dos sentimentos de vergonha e ansiedade ocorriam e possivelmente prejudicavam o desempenho das atletas. Após análise dos resultados pode-se concluir que as manifestações de ansiedade foram mais evidenciadas se comparadas as de vergonha. Notamos que, as preocupações quanto ao desempenho e aos erros que podem ser cometidos são muito evidenciados na equipe. A vergonha-meta... (Resumo completo, clicar acesso eletrônico abaixo)
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Este trabalho teve como objetivo principal avaliar a influência de um período de chuvas intensas sobre as assembléias zooplanctônicas e variáveis limnológicas dos reservatórios de Chavantes e Salto Grande. Em função das precipitações elevadas, o tempo de residência da água durante o período de verão 2009/2010 (período imediatamente anterior às coletas) foi o mais baixo da década (1999-2010), tanto para Chavantes como para Salto Grande. As variáveis analisadas foram: transparência, temperatura, oxigênio dissolvido, pH, condutividade elétrica, turbidez, material em suspensão, clorofila a e nutrientes (nitrogênio total, fósforo total e silicato dissolvido). A turbidez foi a variável que mais se alterou, apresentando um aumento notável com o incremento das chuvas. A transparência e o material em suspensão também apresentaram grande alteração, sendo que a primeira variável diminuiu com a maior quantidade de chuvas e a segunda aumentou. Os valores de fósforo total e silicato dissolvido diminuíram com o aumento das chuvas, sendo que para o silicato a variação foi mais expressiva e para o fósforo o decréscimo foi mais acentuado em Chavantes do que em Salto Grande. Para as outras variáveis limnológicas, bem como para o zooplâncton, os dois reservatórios apresentaram comportamentos diferentes quanto às alterações pluviométricas. A temperatura variou mais em Chavantes. Condutividade elétrica e pH apresentaram relação com a temperatura e o oxigênio dissolvido não teve muita alteração com as mudanças pluviométricas. Os atributos analisados para o zooplâncton foram riqueza, composição, abundância (total, por táxon e das fases de desenvolvimento) e diversidade. Comparando os dois reservatórios estudados, foi observado que em Chavantes o aumento pluviométrico teve efeito muito ...(Resumo completo, clicar acesso eletrônico abaixo)
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O Bacilo de Calmette e Guérin (BCG) é utilizado em muitos países como profilaxia contra a tuberculose. Tem sido sugerido que esta vacina possa determinar, através de atividade imunomoduladora, proteção também contra outras patologias como alergia e doenças autoimunes. Entretanto, este assunto é ainda bastante controverso. A proposta desta investigação foi avaliar o efeito da imunização prévia com BCG no desenvolvimento do diabetes experimental do tipo I em camundongos C57BL/6. Para isto foram realizados três protocolos objetivando caracterizar a resposta imune desencadeada por BCG, padronizar o diabetes experimental tipo I e avaliar o efeito do BCG neste modelo de diabetes. A imunização desta cepa de camundongos com BCG determinou produção discreta de IFN- mas produção significativa de IL-10 após estímulo in vitro das culturas com hsp65 recombinante (rhsp65). Os ensaios de padronização indicaram que o protocolo de inoculação de streptozotocina adotado determinou um quadro de diabetes típico caracterizado por glicemia elevada e graus variáveis de insulite. A imunização com BCG antes da indução do diabetes não alterou de forma significativa o peso, os níveis glicêmicos e a intensidade do processo inflamatório. Os resultados obtidos sugerem que nas condições experimentais adotadas, o BCG não alterou o desenvolvimento do diabetes tipo I induzido por STZ em camundongos C57BL/6