Interação genótipo vs formas de inoculação com Azospirillum brasilense em milho

Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV

Controle genético da resistência a Meloidogyne incognita em Cucumis melo L

Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV

Cassava wastewater as carbon source for the polyhydroxybutyrate production by Azotobacter vinelandii CCT 2841

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Bacillus subtilis na qualidade dos pintinhos ao nascer, desempenho e características intestinais dos frangos

Pós-graduação em Zootecnia - FCAV

Inoculação de sementes com Azospirillum brasilense e Rhizobium tropici e adubação nitrogenada em cobertura em feijoeiro de inverno irrigado em sistema plantio direto

Pós-graduação em Agronomia - FEIS

Meloidogyne inornata em feijoeiro: aspectos biológicos e reação de cultivares

Pós-graduação em Agronomia (Proteção de Plantas) - FCA

Coberturas vegetais e doses de nitrogênio, associadas à inoculação com Azospirillum brasilense, no cultivo do milho na região de Cerrado

Pós-graduação em Agronomia - FEIS

Imunomodulação por Actigen® em tilápia-do-nilo (Oreochromis niloticus): Respostas fisiológicas e desempenho produtivo após desafio com LPS

Pós-graduação em Ciência e Tecnologia Animal - FEIS

Bactérias promotoras de crescimento e adubação nitrogenada no crescimento inicial de cana-de-açúcar proveniente de mudas pré-brotadas

The objective of this work was to evaluate the effects of the inoculation with plant growth-promoting bacteria on the formation of sugarcane pre-sprouted seedlings, originated from individual buds, and to quantify the initial growth of these seedlings in association with nitrogen application, in a low fertility soil. Two experiments were carried out: one in greenhouse conditions, with a time span of 50 days, and the other in vases under field conditions, with a time span of 180 days. In both experiments, a randomized complete block design was used with a factorial arrangement of 2x3, in the first experiment - with or without inoculation, and with three quantities of bud reserve -, and of 2x2x4, in the second one - with or without inoculation, with or without nitrogen, evaluated in four times: at 45, 90, 135, and 180 days. The inoculant had effect on the initial growth of the pre-sprouted seedlings, increasing sprouting speed and dry matter accumulation on roots and shoots, regardless of the bud reserve amount. In the second experiment, the inoculant increased the initial growth of shoots and root system up to 180 days after transplantation, increasing height, tillering, stalk diameter, dry matter production of stalks and straw, and root length, regardless of nitrogen application. The inoculant has a positive physiological effect on plant growth.

Energy deficit does not affect immune responses of experimentally infected pacu (Piaractus mesopotamicus)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Evaluation of Brazilian sugarcane genotypes for resistance to Sugarcane mosaic virus under greenhouse and field conditions

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Gallus gallus domesticus are resistant to infection with Neospora caninum tachyzoites of the NC-1 strain

The aim of this study was to experimentally evaluate infection in Gallus gallus domesticus with Neospora caninum tachyzoites of the NC-1 strain. Experimental infection was conducted in 90-day-old chickens, embryonated eggs and bioassays in dogs. In the first experiment, poults were randomly divided into four groups. Groups I and II were provided feed with coccidiostat, whereas groups III and IV received feed without coccidiostat. When the poults from groups I and III reached 90 days of age, they received a subcutaneous inoculation of N. caninum. Once the hens entered their egg-laying period, during the following 30 days, the eggs were collected, identified, weighed and placed in an incubator. On the 70th day after inoculation, all animals, including the chicks, were euthanized. Tissue samples from the adult poultry and chicks were collected for histopathology, immunohistochemistry (IHC) and PCR. Brain tissue and pectoral muscle samples from infected birds were fed to two dogs. Notably, the average weight of the group III eggs was lower than that of the group IV eggs (p <0.05). No changes consistent with infection in adult poultry or chicks were detected by histopathology or IHC; moreover, no amplified parasite DNA was detected in the birds'tissues or dogs'feces. No dog eliminated oocysts. In the second experiment, the embryonated chicken eggs were inoculated with 1 x 10(2) N. caninum tachyzoites, on the 10th day of incubation, and chicks born from these eggs were housed in boxes suitable for the species and received commercial feed and distilled water ad libitum. On the 30th day after infection (DAI), the poultry were euthanized, and their organs were processed as described in experiment I. The amplification of parasite DNA was observed in the spleen and pectoral muscles of one of the birds. The ingestion of bird tissues by dogs did not result in oocyst elimination. These results indicate that the parasite may have been eliminated by the host and that the use of tachyzoites to induce chronic disease might be a poor source for hens. (C) 2014 Elsevier B.V. All rights reserved.

Resistência de porta-enxertos de cucurbitáceas a nematóides e compatibilidade da enxertia em melão

Nematodes severely attack net melon plants under protected cultivation conditions. The objective of this research was to select rootstocks with resistance to Meloidogyne incognita and M. javanica. The experiment was carried out under greenhouse conditions from October 2010 to April 2011 in Jaboticabal, Sao Paulo state, Brazil. Thirty-three cucurbitaceous genotypes were investigated as rootstocks; melons: CNPH 01-930 (Cucumis melo var. flexuosus), CNPH 01-962, 01-963 CNPH (Cucumis melo var. conomon), cvs. Gaucho Redondo, Gaucho Comprido, Redondo Amarelo, Gulfcoast, Chilton, Bonus no. 2, Fantasy; watermelons: cv. Charleston Gray, Progenie da Coreia (Citrullus lanatus); pumpkins: cvs. Mra. Ma, Ornamental, Howden, Mammoth, Kururu, Goianinha (Cucurbita moschata); gourd: Abobora de Porco, cvs. Maranhao, Brasileirinha (Lagenaria siceraria); squash: cv. Pataca Gigante (Cucurbita maxima); cucumber: cvs. Caipira, Branco Meio Comprido, Curumim (Cucumis sativus); loofah: Metro, Semente Branca, Semente Preta (Luffa cylindrica); wax gourd (Benincasa hispida); pumpkin rootstock: Hybrid cv. Keij; snake gourd (Trichosanthes cucumerins) and musk cucumber (Sicana odorifera). To evaluate the resistance, seedlings were transplanted to pots and the root inoculated with 3,000 eggs and second stage juveniles of M. incognita and M. javanica. Fifty days after the inoculation, the plants were evaluated for nematode resistance by means of the reproduction factor. The grafting compatibility between net melon cvs. Bonus no. 2 and Fantasy and the rootstocks previously characterized as resistant were evaluated by means of 60 graftings. CNPH 01-962, CNPH 01-963 and melon 'Gaucho Redondo', were considered resistant to M. incognita. Melon 'Redondo Amarelo', watermelon 'Charleston Gray', watermelon Progenie da Coreia, Trichosanthes cucumerins were considered resistant to M. javanica. Benincasa hispida was resistant to M. javanica and M. incognita. The compatibility between net melons and resistant rootstocks was higher than 98%.

A virtual experiment for the detection of specific features in the frequency response of a coupled nonlinear and linear oscillator

This paper presents an investigation into some practical issues that may be present in a real experiment, when trying to validate the theoretical frequency response curve of a two degree-of-freedom nonlinear system consisting of coupled linear and nonlinear oscillators. Some specific features, such as detached resonance curves, have been theoretically predicted in multi degree-of-freedom nonlinear oscillators, when subject to harmonic excitation, and the system parameters have been shown to be fundamental in achieving such features. When based on a simplified model, approximate analytical expression for the frequency response curves may be derived, which may be validated by the numerical solutions. In a real experiment, however, the practical achievability of such features was previously shown to be greatly affected by small disturbances induced by gravity and inertia, which led to some solutions becoming unstable which had been predicted to be stable. In this work a practical system configuration is proposed where such effects are reduced so that the previous limitations are overcome. A virtual experiment is carried out where a detailed multi-body model of the oscillator is assembled and the effects on the system response are investigated.

Effects of Yersinia enterocolitica O:3 derivatives on B lymphocyte activation in vivo

The potential sequelae of intestinal infection with Yersinia enterocolitica include reactive arthritis, erythema nodosum, Reiter's syndrome and other autoimmune diseases. The role of the immune response in the pathogenesis of these diseases has not been fully defined, but autoimmune manifestations may be a consequence of the increase in autoantibodies as a result of polyclonal B-cell activation induced by Yersinia. We investigated the effects of Y enterocolitica 0:3 derivatives on B lymphocyte activation in vivo. Groups of five specific pathogen free (SPF) Swiss mice were inoculated with bacterial cell extract, Yersinia outermembrane proteins (Yops) or lipopolysaccharide (LPS) obtained from Y enterocolitica 0:3 and their immunoglobulin-secreting spleen cells were detected by isotype-specific protein A plaque assay. The presence of specific anti-Yersinia antibodies and autoantibodies was determined in mouse sera by ELISA. In all experiments a marked increase in the number of secretory cells of different isotypes was observed as early as the third day after inoculation. IgG and IgM anti-Yersinia antibodies were detected in the sera of all inoculated mice, and autoantibodies against myosin in the sera of those inoculated with bacterial cell extract. The sera from animals stimulated with LPS reacted with myelin, actin and laminin, while the sera from mice inoculated with Yops reacted with myelin, thyroglobulin and cardiolipin. These results suggest that SPF Swiss mice inoculated with any one of the Y enterocolitica derivatives tested exhibited polyclonal activation of B lymphocytes as a result of stimulation by various bacterial components and not only LPS stimulation.