Protein kinase C chimeras: catalytic domains of alpha and beta II protein kinase C contain determinants for isotype-specific function.

Protein kinase C (PKC) is involved in the proliferation and differentiation of many cell types. In human erythroleukemia (K-562) cells, the PKC isoforms alpha and beta II play distinct functional roles. alpha PKC is involved in phorbol 12-myristate 13-acetate-induced cytostasis and megakaryocytic differentiation, whereas beta II PKC is required for proliferation. To identify regions within alpha and beta II PKC that allow participation in these divergent pathways, we constructed chimeras in which the regulatory and catalytic domains of alpha and beta II PKC were exchanged. These PKC chimeras can be stably expressed, exhibit enzymatic properties similar to native alpha and beta II PKC in vitro, and participate in alpha and beta II PKC isotype-specific pathways in K-562 cells. Expression of the beta/alpha PKC chimera induces cytostasis in the same manner as overexpression of wild-type alpha PKC. In contrast, the alpha/beta II PKC chimera, like wild-type beta II PKC, selectively translocates to the nucleus and leads to increased phosphorylation of the nuclear envelope polypeptide lamin B in response to bryostatin-1. Therefore, the catalytic domains of alpha and beta II PKC contain determinants important for alpha and beta II PKC isotype function. These results suggest that the catalytic domain represents a potential target for modulating PKC isotype activity in vivo.

Specific and reversible immobilization of proteins tagged to the affinity polypeptide C-LytA on functionalized graphite electrodes

We have developed a general method for the specific and reversible immobilization of proteins fused to the choline-binding module C-LytA on functionalized graphite electrodes. Graphite electrode surfaces were modified by diazonium chemistry to introduce carboxylic groups that were subsequently used to anchor mixed self-assembled monolayers consisting of N,N-diethylethylenediamine groups, acting as choline analogs, and ethanolamine groups as spacers. The ability of the prepared electrodes to specifically bind C-LytA-tagged recombinant proteins was tested with a C-LytA-β-galactosidase fusion protein. The binding, activity and stability of the immobilized protein was evaluated by electrochemically monitoring the formation of an electroactive product in the enzymatic hydrolysis of the synthetic substrate 4-aminophenyl β-D-galactopyranoside. The hybrid protein was immobilized in an specific and reversible way, while retaining the catalytic activity. Moreover, these functionalized electrodes were shown to be highly stable and reusable. The method developed here can be envisaged as a general, immobilization procedure on the protein biosensor field.

Crash protection systems for handicapped school and transit bus occupants. Volume III: appendices A, B, C and D. Interim report.

National Highway Traffic Safety Administration, Washington, D.C.

Asiatic Art, VII century B.C.-XVIII century A.D., including sculpture in bronze & stone, ceramics, miniatures and textiles.

Exhibition and sale at the American Art Galleries, New York.

East and west through fifteen centuries; being a general history from B.C. 44 to A.D. 1453,

No more published.

A B C de l'apiculture; encyclopédie de tout ce qui a rapport à l'abeille, miel, ruches, instruments, plantes mellifères, etc., résultats d'observations et d'expériences de milliers d'apiculteurs, contrôlées ensuite par un travail pratique dans nos propres ruchers.

p. 518-524 advertising.

L.M.B.C. memoirs on typical British marine plants and animals

Mode of access: Internet.

Las leyes de todos los reynos de Castilla abreuiadas y reduzidas en forma de Reportorio decisiuo por la orden del A.B.C. /

Portada a dos tintas con escudo y orla xilográficos.

El A.B.C. de la visión intuitiva ó principios de la visión relativamente á los tamaños /

Contiene: Parte 1º y Parte 2º

The Tyrolien lyre: a glee book, consisting of easy pieces arranged mostly for soprano, alto, tenor, and base [sic] voices, with and without piano-forte accompaniments; comprising a complete collection of solos, duetts, trios, quartetts, quintetts, choruses, &c. for the use of societies, schools, clubs, choirs, and the social circle,

Mode of access: Internet.

A victor of Salamis: a tale of the days of Xerxes, Leonidas, and Themistocles,

Mode of access: Internet.

The Roman empire : B. C. 29-A. D. 476 /

Includes index.

Refranes, y modos de hablar castellanos : con los latinos que les corresponden, y la glosa, y explicación de los que tienen necesidad de ella : Con un indice de los adgios latinos, á los quales corresponden los castellanos, que van puestos en el libro por el órden de A. B. C. /

Palau,

The homosexual in literature; a chronological bibliography, circa 700 B.C.-1958,

Cover title.

De elegantia Caesaris, sive, De commentarium de b. G. et de b. c. differentiis animadersiones ...

"Vita."