Changes in Cell Wall Composition during Ripening of Grape Berries1

Cell walls were isolated from the mesocarp of grape (Vitis vinifera L.) berries at developmental stages from before veraison through to the final ripe berry. Fluorescence and light microscopy of intact berries revealed no measurable change in cell wall thickness as the mesocarp cells expanded in the ripening fruit. Isolated walls were analyzed for their protein contents and amino acid compositions, and for changes in the composition and solubility of constituent polysaccharides during development. Increases in protein content after veraison were accompanied by an approximate 3-fold increase in hydroxyproline content. The type I arabinogalactan content of the pectic polysaccharides decreased from approximately 20 mol % of total wall polysaccharides to about 4 mol % of wall polysaccharides during berry development. Galacturonan content increased from 26 to 41 mol % of wall polysaccharides, and the galacturonan appeared to become more soluble as ripening progressed. After an initial decrease in the degree of esterification of pectic polysaccharides, no further changes were observed nor were there large variations in cellulose (30–35 mol % of wall polysaccharides) or xyloglucan (approximately 10 mol % of wall polysaccharides) contents. Overall, the results indicate that no major changes in cell wall polysaccharide composition occurred during softening of ripening grape berries, but that significant modification of specific polysaccharide components were observed, together with large changes in protein composition.

Identification of Acremonium isolates from grapevines and evaluation of their antagonism towards Plasmopara viticola

Some endophytic fungal genera in Vitis vinifera, including Acremonium, have been reported as antagonists of Plasmopara viticola. Endophytic Acremonium isolates from an asymptomatic grapevine cultivar Inzolia from Italy were identified by morphological features and multigene phylogenies of ITS, 18S and 28S genes, and their intra-specific genomic diversity was analyzed by RAPD analysis. Culture filtrates (CFs) obtained from Acremonium isolates were tested in vitro for their inhibitory activity against the P. viticola sporangia germination. Among 94 isolates, 68 belonged to the Acremonium persicinum and 26 to the Acremonium sclerotigenum. RAPD analysis grouped the A. persicinum isolates into 15 clusters and defined 31 different strains. The A. sclerotigenum isolates, instead, were clustered into 22 groups and represented 25 strains. All A. persicinum CFs inhibited sporangia germination of P. viticola, while not all those of A. sclerotigenum had inhibitory effect. A different degree of inhibition was observed between strains of the same species, while some strains of different species showed identical inhibitory effect. No correlation was found between RAPD groups and inhibitory activity in both Acremonium species.

Stabilizing and Modulating Color by Copigmentation: Insights from Theory and Experiment

Natural anthocyanin pigments/dyes and phenolic copigments/co-dyes form noncovalent complexes, which stabilize and modulate (in particular blue, violet, and red) colors in flowers, berries, and food products derived from them (including wines, jams, purees, and syrups). This noncovalent association and their electronic and optical implications constitute the copigmentation phenomenon. Over the past decade, experimental and theoretical studies have enabled a molecular understanding of copigmentation. This review revisits this phenomenon to provide a comprehensive description of the nature of binding (the dispersion and electrostatic components of π–π stacking, the hydrophobic effect, and possible hydrogen-bonding between pigment and copigment) and of spectral modifications occurring in copigmentation complexes, in which charge transfer plays an important role. Particular attention is paid to applications of copigmentation in food chemistry.

II. Butler bills, 1788-1794

The Butler's bills are printed quarterly bill forms that the Butler completed with his name, the name of the student, the specific quarter and its ending date, and the total owed for "permitted articles." In some years the form included a space to input the amount for "Wines and other permitted Liquors."

II. Butler bills, 1788-1794

The Butler's bills are printed quarterly bill forms that the Butler completed with his name, the name of the student, the specific quarter and its ending date, and the total owed for "permitted articles." In some years the form included a space to input the amount for "Wines and other permitted Liquors."

Palynology on lake sediments from the Pyrenees

A multi-proxy palaeoecological investigation including pollen, plant macrofossil, radiocarbon and sedimentological analyses, was performed on a small mountain lake in the Eastern Pyrenees. This has allowed the reconstruction of: (1) the vegetation history of the area based on five pollen diagrams and eight AMS14C dates and (2) the past lake-level changes, based on plant macrofossil, lithological and pollen analysis of two stratigraphical transects correlated by pollen analysis. The palaeolake may have appeared before the Younger Dryas; the lake-level was low and the vegetation dominated by cold steppic grasslands. The lake-level rose to its highest level during the Holocene in the Middle Atlantic (at ca. 5060±45 b.p.). Postglacial forests (Quercetum mixtum and Abieto-Fagetum) developed progressively in the lower part of the valley, while dense Pinus uncinata forests rapidly invaded the surroundings of the mire and remained the dominant local vegetation until present. The observed lowering of the lake levels during the Late Atlantic and the Subboreal (from 5060 ± B.P. to 3590±40 b.p.) was related to the overgrowth of the mire. The first obvious indications of anthropogenic disturbances of the vegetation are recorded at the Atlantic/Subboreal boundary as a reduction in the forest component, which has accelerated during the last two millennia.

Sylvan Township and Chelsea (Mich.) residences, ca. 1874

Left to right: Res. of Henry Warren, Sec. 33, Webster Tp. Mich.; Res. of Skidmore, Sec. 17, Lyndon Tp. Mich.; Res. of J.H. Collins, Sec. 4, Lyndon Tp. Mich.; Res. of Wm. A. Jones, Sec. 32, Dexter Tp. Mich.; Res. of Hanson Sesions, Sec.2, Northfield Tp. Mich.; Res. of A.E. Collins, Sec. 4, Lyndon Tp. Mich. Drawn and Compiled by Cyrus Wheelock. Publication information: Chicago, Ill. : Everts & Stewart, 1874.

Handbook of courses open to women in British, continental, and Canadian universities /

Mode of access: Internet.

The people's own book of recipes and information for the million : containing directions for the preservation of health, for the treatment of the sick and the conduct of the sick-room : with a full discussion of the more porminent diseases that afflict the human family, with full directions for their rational treatment : also, 1000 practical and useful recipes, embracing every department of domestic economy and human industry : with copious notes and emendations, explanatory and suggestive /

Recipes for domestic wines, cider, etc., on p. 306-315.

International congress on the prevention and repression of crime, including penal and reformatory treatment : preliminary report of the commissioner appointed by the President to represent the United States in the congress in compliance with a joint resolution of March 7, 1871.

E. C. Wines, commissioner.

Trade agreement digests. Prepared for use in connection with trade agreement negotiations.

Some of the vols. Issued in pts.

The nomenclature of crime; or An analytical list of offenses against the statutes of the United States and of the states of the federal union.

Letter of transmittal addressed to Gen. Francis A. Walker, superintendent of census, by Fred. H. Wines, special agent.

Report of the ... annual State Viticultural Convention.

Reports of 3rd-5th conventions pub. by the San Francisco Merchant.

Il castello del Buon Consiglio a Trento : documenti concernente la fabbrica nel perioda clesiano 1527-1536 /

Includes bibliographical references.