Description of a rat palatal acrylic plate that can be relined

Purpose: The objective of this article is to describe a method to construct an intraoralacrylic device that permits a reline material to be added to the inner surface of thepalatal plate.Materials and Methods: Fifteen 60-day-old adult female rats (Rattus NorvegicusAlbinus Wistar), weighing 150 to 250 g were used for this study and allocated to threegroups (n = 5): G1, animals wearing a heat-polymerized acrylic resin palatal plate(Lucitone 550) for 14 days; G2, animals wearing a heat-polymerized acrylic resinpalatal plate (Lucitone 550) relined with Tokuyama Rebase II for 14 days; and G3,animals maintained under the same conditions as the experimental groups, withoutwearing palatal plates for 14 days. The manipulation of the animals followed theguidelines of the Brazilian College of Animal Experimentation, under the approval ofthe animal ethics committee of the State University of Ponta Grossa. The palatal platescovered the whole palate, were fixed in the molar region with light-cured resin, andwere kept there for 14 days. The animals received a paste diet and water ad libitum.Before and after the trial period, the rats were weighed individually on a precisionscale. Statistical analysis was performed using a two-way analysis of variance (α =0.05) test for comparison of the animals’ weight (g) at time 0 and after 14 days ofusing the palatal plate.Results: No statistical differences were observed regarding the weight of the animalsamong the experimental groups in the study.Conclusions: The individual master impressions, the molar teeth coverage, and themethod of cementation with nonadhesive composite resin provided good stability forthe palatal plate showed in this study, not disturbing the eating habits and nutritionof the animals. This model seems reproducible, offering adequate histopathologicalevaluation. Differences in tissue morphology exist between the animals that used thepalatal plate and the animals that did not use this device. Use of these palatal platescould clarify how prostheses bring changes in the palatal mucosa of users.

Effect of post-polymerization heat treatment on a denture base acrylic resin: histopathological analysis in rats

This work examined the histological effects, on the rat palatal mucosa, of a denture base acrylic resin, submitted or not to a post-polymerization heat-treatment. Methods: Fifteen adult female Wistar rats, with sixty days old, weighting 150 g – 250 g were divided in G1: animals being maintained under the same conditions as the experimental groups following described, but without the use acrylic palatal plates (control group); G2: use of heat-polymerized acrylic resin palatal plates made of Lucitone 550; G3: use of palatal plates identical to G2, but subjected to a post-polymerization treatment in a water bath at 55°C for 60 min. The plates covered all the palate and were fixed in the molar region with light-cured resin, thus being kept there for 14 days. After the sacrifice, the palate was removed, fixed in formaldehyde 10% and decalcified with EDTA. Sections were stained using haematoxylin and eosin. Images in duplicate were made from the central region of the cuts, to measure the thickness (μm) of the keratin layers (TKC), epithelium total (TET) and connective tissue (TCC). Statistical analyses were carried out by one-way ANOVA and Tukey post-tests (α=0.05). Results: According to the results there was significant difference in the thickness of keratin between G2 and G3, with G1 having the intermediate value and similar to the other groups. There was a significant difference in the connective tissue with G3

Preventive effect of a high fluoride toothpaste and arginine-carbonate toothpaste on dentinal tubules exposure followed by acid challenge: a dentine permeability evaluation

Coordenação de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)

Efeito do fumo sobre os tecidos periodontais: uma revisão de literatura

Smoking is one of the most important risk factors for periodontal disease. Increased destruction of periodontal tissues has been observed in smokers. Studies show changes caused by different cigarette components, morphological, microbiological and immunological alterations which could lead to clinical changes. However, the pathophysiology of smoking on periodontal tissues are not completely understood. Therefore, this article aims to present a review of the literature addressing the changes on the periodontal tissues caused by the main components of the smoke observed in recent studies.

In vitro evaluation of Biosilicate® dissolution on dentin surface: a SEM analysis

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Qualidade hídrica e morfometria da microbacia do córrego da Fazenda Glória, Taquaritinga-SP

Pós-graduação em Agronomia (Ciência do Solo) - FCAV

Condomínios residenciais fechados: a urbanização do Grupo Encalso Damha em São Carlos - SP

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Outbreak control and clinical, pathological, and epidemiological aspects and molecular characterization of a bovine herpesvirus type 5 on a feedlot farm in S(a)over-tildeo Paulo State

This paper describes the control, epidemiological, pathological, and molecular aspects of an outbreak of meningoencephalitis in calves due to bovine herpesvirus 5 at a feedlot with 540 animals in Sa (a) over tildeo Paulo State, Brazil. The introduction of new animals and contact between the resident animals and the introduced ones were most likely responsible for virus transmission. Bovine herpesvirus 1 vaccine was used, resulting in the efficacy of the outbreak control, although two bovine herpesvirus 1 positive animals, vaccinated and revaccinated, presented meningoencephalitis, thereby characterizing vaccinal failure.

Different bacterial models for in vitro induction of non-cavitated enamel caries-like lesions: microhardness and polarized light miscroscopy analyses

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Morphological aspects of Henneguya aequidens n. sp (Myxozoa: Myxobolidae) in Aequidens plagiozonatus Kullander, 1984 (Teleostei: Cichlidae) in the Amazon region, Brazil

A new species of Myxosporea, Henneguya aequidens sp. n. (Myxozoa: Myxobolidae), was described based on its ultrastructural features. This is a parasite of the freshwater fish Aequidens plagiozonatus, in the Peixe-boi River, Para, Brazil. This parasite was found in the gills, in the form of whitish ellipsoid cysts with mature spores inside them. The average spore body was 15 +/- 0.9 mu m in length (n = 30) and 6 +/- 0.8 mu m in width (n = 30), and the tail measured 27 +/- 0.5 mu m in length (n = 15). The spores showed typical features of the genus Henneguya with two valves of equal size and two symmetrical polar capsules of 3 +/- 0.3 mu m in length and 2 +/- 0.3 mu m in width. Each polar capsule had a polar filament forming a helix from the apical region to the polar caps, with four to six turns. Based on the ultrastructural differences in morphology of these spores, the location of the parasite, and its host specificity, this parasite was described as a new species.

Paradoxical effects of bovine somatotropin treatment on the ovarian follicular population and in vitro embryo production of lactating buffalo donors submitted to ovum pick-up

The aim of the present study was to evaluate the effect of bovine somatotropin (bST; 500 mg) administration on lactating buffalo donors submitted to two different ovum pick-up (OPU) and in vitro embryo production schemes with a 7 or 14 d intersession OPU interval. A total of 16 lactating buffalo cows were randomly assigned into one of four experimental groups according to the bST treatment (bST or No-bST) and the OPU intersession interval (7 or 14 d) in a 2 x 2 factorial design (16 weeks of OPU sessions). The females submitted to OPU every 14d had a larger (P < 0.001) number of ovarian follicles suitable for puncture (15.6 +/- 0.7 vs. 12.8 +/- 0.4) and an increased (P = 0.004) number of cumulus-oocyte complexes (COCs) recovered (10.0 +/- 0.5 vs. 8.5 +/- 0.3) compared to the 7 d interval group. However, a 7 or 14 d interval between OPU sessions had no effect (P = 0.34) on the number of blastocysts produced per OPU (1.0 +/- 0.1 vs. 13 +/- 0.2, respectively). In addition, bST treatment increased (P < 0.001) the number of ovarian follicles suitable for puncture (15.3 +/- 0.5 vs. 12.1 +/- 0.4) but reduced the percentage (18.9% vs. 10.9%; P = 0.009) and the number (1.4 +/- 0.2 vs. 0.8 +/- 0.1; P = 0.003) of blastocysts produced per OPU session compared with the non-bST-treated buffaloes. In conclusion, the 14d interval between OPU sessions and bST treatment efficiently increased the number of ovarian follicles suitable for puncture. However, the OPU session interval had no effect on embryo production, and bST treatment reduced the in vitro blastocyst outcomes in lactating buffalo donors.

Total sugars in atmospheric aerosols: an alternative tracer for biomass burning

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Energy values of traditional ingredients and sugarcane yeast for laying hens

An experiment was conducted to determine the chemical composition and apparent metabolizable energy (AME) and apparent metabolizable energy corrected for nitrogen balance (AMEn) values of corn, soybean meal (SBM), soybean oil (SO) and sugarcane yeast (SY) (Saccharomyces cerevisiae). A metabolism trial was performed with 120 Dekalb White laying hens at 65 weeks of age, using the method of total excreta collection. Birds were housed in metabolism cages and distributed according to a completely randomized design into five treatments with, six replicates of four birds each. The experimental period consisted of four days of adaptation and four days of excreta collection. The experimental diets included: a reference diet based on corn and SBM and four test diets containing 40% corn, 30% SBM, 10% SO or 30 % SY. The chemical compositions of the tested ingredients, expressed on "as-is" basis were: 86.9, 87.29, 87.32 and 99.5% dry matter; and 3.51, 2.08, 99.31 and 0.03 ether extract for corn, SBM, SO and SY, respectively. Corn, SBM, and SO presented 7.33, 43.61 and 24.64% crude protein, and 0.58, 5.07 and 6.77% ash, respectively; and crude fiber contents of corn and SBM were, respectively, 2.24% and 3.56%. The following AME and AMEn (kcal/kg dry matter) values were obtained: 3,801 and 3,760 kcal/kg for corn, 2,640 and 2,557 kcal/kg for SBM, 8,952 and 8,866 kcal/kg for SO, and 1,023 and 925 kcal/kg for sugarcane yeast, respectively.

Influence of storage times on bond strength of resin cements to root canal

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Evaluation of the owner's perception in the use of homemade diets for the nutritional management of dogs

Many dog owners see homemade diets as a way of increasing the bond with their pets, even though they may not have the convenience of commercial diets. Modifications of ingredients, quality and proportion might change the nutritional composition of the diet, generating nutritional imbalances. The present study evaluated how dog owners use and adhere to homemade diets prescribed by veterinary nutritionists over an extended period of time. Forty-six owners of dogs fed a homemade diet for at least 6 months were selected for the present study. Owners were invited to answer questions by first reading all possible answers and then selecting the one that best indicated their opinion. The results were evaluated through descriptive statistics. Thirty-five owners (76·1 %) found that the diets are easy to prepare. Fourteen owners (30·4 %) admitted to modifying the diets, 40 % did not adequately control the amount of provided ingredients, 73·9 % did not use the recommended amounts of soyabean oil and salt, and 34·8 % did not correctly use the vitamin, mineral or amino acid supplements. Twenty-six owners (56·5 %) reported that their dogs refused to eat at least one food item. All of these alterations make the nutritional composition of the diets unpredictable and likely nutritionally imbalanced. Although homemade diets could be a useful tool for the nutritional management of dogs with certain diseases, not all owners are able to appropriately use this type of diet and adhere to it for an extended period of time and this limitation needs to be considered when recommending the use of homemade diets.