647 resultados para Sundström, Zacharias
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Marca tip. en port.
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The transposon Tn5090/Tn402 encodes a 559 amino acid transposase, TniA, with a DDE motif. Gel mobility shifting and cleavage protection analysis with DNase I and hydroxyl radical probes revealed that TniA binds to multiple repeat sequences on either terminus of Tn5090/Tn402. Four of these TniA-binding 19mers occurred on the left-hand (t) end and two on the right-hand (i) end. Hydroxyl radical cleavage protection demonstrated the presence of 3–6 bp contact sequences on one face of the DNA helix. The binding pattern and organisation of repeats suggested parallels between Tn5090/Tn402 and Mu, which controls its transpositional activity in the assembly step of a higher order transpososome complex. The complex terminal structure and genes of transposase and nucleotide-binding proteins in tandem are hallmarks of the handful of Mu-like elements that are known to date.
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We report studies of energy transfer from the 800-nm absorbing pigment (B800) to the 850-nm absorbing pigment (B850) of the LH2 peripheral antenna complex and from LH2 to the core antenna complex (LH1) in Rhodobacter (Rb.) sphaeroides. The B800 to B850 process was studied in membranes from a LH2-reaction center (no LH1) mutant of Rb. sphaeroides and the LH2 to LH1 transfer was studied in both the wild-type species and in LH2 mutants with blue-shifted B850. The measurements were performed by using approximately 100-fs pulses to probe the formation of acceptor excitations in a two-color pump-probe measurement. Our experiments reveal a B800 to B850 transfer time of approximately 0.7 ps at 296 K and energy transfer from LH2 to LH1 is characterized by a time constant of approximately 3 ps at 296 K and approximately 5 ps at 77 K. In the blue-shifted B850 mutants, the transfer time from B850 to LH1 becomes gradually longer with increasing blue-shift of the B850 band as a result of the decreasing spectral overlap between the antennae. The results have been used to produce a model for the association between the ring-like structures that are characteristic of both the LH2 and LH1 antennae.
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The trans-activation response element (TAR) found near the 5' end of the viral RNA of the human immunodeficiency virus contains a 3-nt bulge that is recognized by the virally encoded trans-activator protein (Tat), an important mediator of transcriptional activation. Insertion of the TAR bulge into double-stranded RNA is known to result in reduced electrophoretic mobility, suggestive of a bulge-induced bend. Furthermore, NMR studies indicate that Arg causes a change in the structure of the TAR bulge, possibly reducing the bulge angle. However, neither of these effects has been quantified, nor have they been compared with the effects of the TAR-Tat interaction. Recently, an approach for the quantification of bulge-induced bends has been described in which hydrodynamic measurements, employing the method of transient electric birefringence, have yielded precise estimates for the angles of a series of RNA bulges, with the angles ranging from 7 degrees to 93 degrees. In the current study, transient electric birefringence measurements indicate that the TAR bulge introduces a bend of 50 degrees +/- 5 degrees in the absence of Mg2+. Addition of Arg leads to essentially complete straightening of the helix (to < 10 degrees) with a transition midpoint in the 1 mM range. This transition demonstrates specificity for the TAR bulge: no comparable transition was observed for U3 or A3 (control) bulges with differing flanking sequences. An essentially identical structural transition is observed for the Tat-derived peptide, although the transition midpoint for the latter is near 1 microM. Finally, low concentrations of Mg2+ alone reduce the bend angle by approximately 50%, consistent with the effects of Mg2+ on other pyrimidine bulges. This last observation is important in view of the fact that most previous structural/binding studies were performed in the absence of Mg2+.
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Back Row: Mgr. Keith Webster, Video Coordinator Dave Garlow, Head Trainer Russ Miller, g.a.'s: Paul Alexander, Bill Sheridan, Jim Herrmann, John Johnson, Randy Fichtner, Kevin Kalinich, Tr. Paul Schmidt, Tr. Rex Thompson, Equipment Mgr. Jon Falk, Assistant Denny Morgan, Graduate Assistant John Ferens
11th Row: Assistant coaches: Alex Agase, Cam Cameron, Jerry Meter, Tom Reed, Elliott Uzelac, Gary Moeller, Lloyd Carr, Bill Harris, Jerry Hanlon, Tirrel Burton, Mike Gittleson, Recruiting Coord. Fritz Seyferth, Admin. Ass't. Cliff Dochterman
10th Row: Geoff Bissell, Mark Gutzwiller, Steve Woroniecki, Dave Knight, Steve Zacharias, Todd Plate, Matt McCoy, Greg Zeigler, Byron Lawson, Sean Eastman, Scott Harrala, Joel Boyden, Doug Matton
9th Row: Chris Calloway, Chris Horn, Scott Smykowski, Trey Walker, Jeff Tubo, Curtis Feaster, Mike Kerr, Marc Ramirez, T.J. Osman, Mike Teeter, Doug Daugherty, Huemartin Robinson
8th Row: John Milligan, Tony Boles, Jarrod Bunch, Greg McMurtry, Marc Spencer, Tom Dohring, Warde Manuel, Timothy Williams, Leroy Hoard, Allen Jefferson, Tracy Williams, David Key
7th Row: Olatide Ogunfiditimi, Keith Cooper, Scott Crawford, Joe Holland, Frank Petroff, Pat Olszewski, John Plantz, Sean LaFountaine, Mike Edwards, Rick Hassel, Ted Harris, Gulam Khan
6th Row: Kyle Anderson, Mark Erhardt, Brian Reid, Keith Mitchell, John Herrmann, Brent White, Dave Weil, Derrick Walker, Michael Taylor, Anthony Mitchell, Pat Fitzgerald, Mike DeBoer, Vince Washington
5th Row: Rick Stites, Ernie Bock, Mike Gillette, Scott Mandel, Mike Kovak, John Willingham, Dave Mandel, John Duerr, J.J. Grant, Don Lessner, Bob Stites
4th Row: Ken Mouton, Ernie Holloway, David Arnold, Jeffrey Brown, Dave Dever, Dave Herrick, Michael Dames, Dave Chester, Bob Cernak, Rick Sutkiewicz, John Kolesar, Allen Bishop
3rd Row: Erik Campbell, Mike Husar, Mark Messner, Jack Walker, Steve Thibert, Andree McIntyre, Andy Borowski, Dave Folkertsma, Tim Schulte, John Vitale, Phil Webb, Phil Logas
2nd Row: Jamie Morris, Monte Robbins, Todd Schulte, Billy Harris, Mark Hammerstein, Paul Jokisch, Bob Perryman, Mike Reinhold, John Elliott, Jerry Quaerna, Carlitos Bostic, Chris Zurbrugg, Head Coach Bo Schembechler
Front Row: Ken Higgins, Pat Moons, Garland Rivers, Ivan Hicks, Andy Moeller, Tony Gant, Jim Harbaugh, Gerald White, Thomas Wilcher, Dieter Heren, Doug Mallory, Russell Rein
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Front Row: Anthony Mitchell, Pat Fitzgerald, David Ritter, Allen Bishop, Chris Zurbrugg, Phil Webb, Jamie Morris, Erik Campbell, Doug Mallory, Bob Cernak, Ernie Bock, Don Lessner, Rick Stites, Bob Stites.
2nd Row: Andy Borowski, Dave Chester, Dave Folkertsma, Michael Dames, John Vitale, Andre McIntyre, John Elliott, Mark Messner, Steve Thibert, Monte Robbins, Billy Harris, John Willingham, Mike Husar, Carlitos Bostic, Bo Schembechler.
3rd Row: Rick Hassel, Bobby Abrams, Derrick Walker, Jeff Brown, David Arnold, Dave Dever, Brent White, John Duerr, Dave Mandel, Scott Mandel, Michael Taylor, Demetrius Brown, John Kolesar, Mike Gillette.
4th Row: Ernie Holloway, Rick Sutkiewicz, Keith Cooper, J.J. Grant, Keith Mitchell, Dean Dingman, Pat Olszewski, David Weil, Joe Holland, John Herrmann, Frank Petroff, Olatide Ogunfitidim, Sean LaFontaine, Mike DeBoer.
5th Row: Vince Washington, Scott Herrala, David Key, Mike Teeter, John Milligan, Greg McMurtry, John Plantz, Joel Boyden, Warde Manuel, Jarrod Bunch, Allen Jefferson, Chris Calloway, Doug Matton, Gulam Khan.
6th Row: Mark Gutzwiller, Jeff Tubo, Marc Spencer, Marc Ramirez, T.J. Osman, Scott Smykowski, Tom Dohring, Doug Daugherty, Mike Kerr, Curtis Feaster, Vada Murray, Tim Williams, Tracy Williams, Trey Walker.
7th Row: Sean Eastman, Byron Lawson, Dave Knight, Todd Plate, Greg Ziegler, Steve Zacharias, Huemartin Robinson, Tony Boles, Chris Horn, Mike Edwards, Stu Duncan, Dave Herrick, Brian Reid, Ken Mouton, Chris D'Esposito.
8th Row: Eric Bush, Wilbur Odom, Erick Anderson, Brian Townsend, Ron Zielinski, Dave Diebolt, Greg Skrepenak, Dave Dingman, Alex Marshall, Chris Bohn, Rusty Fishtner, Ken Sollom, Otis Williams, Ra-Mon Watkins.
9th Row: Shawn Watson, Carlos Smith, Yale VanDyne, Mike Evans, Dave Ritter, Matt Elliott, Dan Jokisch, Mark Soehnlen, Lance Dottin, Neil Simpson, Kevin Owen, Jim Sinclair, Bill Madden, J.D. Carlson, John Rodney.
10th Row: Aaron Studwell, Jon Falk, Mike Gittleson, Mike Walters, Damon Taylor, Leon Morton, Dave Caputo, Brad Moyer, Colin Rudolph, Eric Traupe, David Papp, Fritz Seyferth, Russ Miller, Paul Schmidt, Kevin Kolcheff, Brad Andres.
Back Row: Dennis Morgan, Jeff Long, Jim Herrmann, Bill Harris, Bobby Morrison, Tom Reed, Lloyd Carr, Gary Moeller, Jerry Hanlon, Tirrel Burton, Les Miles, Cam Cameron, Alex Agase, Kevin Kalinich, Randy Fichtner, Dave Garlow, Dennis Blanchard, Charlie Baird.
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Back Row: Jon Falk, Brad Andress, Alozie Okezie, Eddie Azcona, John Albertson, Randy Stark, Chris Hutchinson, Barry Kelley, Erik Knuth, Paul Manning, Pat Maloney, John Woodlock, John Ellison, Curt Mallory, Kevin Heading, Assayan Jordan, Ira Pintel, Phil Bromley, Scott Woolf
7th Row: Pat Perkins, Bob Bland, Mike Filander, John Becker, Doug Cohen, D.J. Brown, Elvis Grbac, Steve Everitt, Rob Doherty, Doug Skene, Joe Cocozzo, Brian Wallace, Mortin Davis, Bill Schaffer, Livetius Johnson, Coleman Wallace, Corwin Brown, Dave Herrick, Dennis Blanchard
6th Row: Russ Miller, Ken Mouton, Jim Plocki, Jon Vaughn, Desmond Howard, Dwayne Ware, Ra-Mon Watkins, Shawn Watson, Dave Caputo, Eric Traupe, James Sinclair, Mike Evans, Matt McCoy, Yale Van Dyne, J.D. Carlson, Bill Madden, Leon Morton, Kevin Kolcheff, Jim Herrmann
5th Row: Paul Schmidt, Jeff Long, Dave Knight, Ron Zielinski, Lance Dottin, Dave Ritter, Erick Anderson, Dave Diebolt, Dan Jokisch, Greg Skrepenak, Alex Marshall, Brian Townsend, Matt Elliott, Chris Bohn, Rusty Fichtner, Marc Soehnlen, Mike Gittleson, Cam Cameron
4th Row: Jon Heacock, Bill Harris, Eric Bush, Otis Williams, Steve Zacharias, Jeff Tubo, Trey Walker, Dean Dingman, Marc Spencer, Scott Smykowski, Marc Ramirez, Doug Daugherty, Tripp Welborne, Neil Simpson, Todd Plate, Wilbur Odom, Ken Sollom, Tirrel Burton, Bobby Morrison
3rd Row: Bob Chmiel, Gulam Khan, Chris Horn, Tracy Williams, Leroy Hoard, Tim Williams, John Milligan, Tom Dohring, Jarrod Bunch, Warde Manuel, Greg McMurtry, Chris Feaster, Mike Teeter, T.J. Osman, Doug Matton, Mark Gutzwiller, Kevin Owen, Tom Reid, Les Miles
2nd Row: Jerry Hanlon, Bobby Abrams, David Key, Joel Boyden, Anthony Mitchell, Rick Hassel, Frank Petroff, Keith Mitchell, Pat Olszewski, David Weil, Joe Holland, Sean LaFountaine, Vincent Washington, Chris Calloway, Allen Jefferson, Tony Boles, Vada Murray, Gary Moeller, Lloyd Carr1st row:Mike Gillette, John Kolesar, John Herrmann, David Arnold, Dave Chester, John Vitale, Mark Messner, Michael Dames, Mike Husar, Jeff Brown, Derrick Walker, J.J. Grant, Brent White, Michael Taylor, Bo Schembechler.
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Back Row: Scott Wolfe, Phil Bromley, Bob Chmiel, Cam Cameron, Les Miles, Tirrel Burton, Jerry Hanlon, Gary Moeller, Lloyd Carr, Tom Reed, Bill Harris, Bobby Morrison, Jim Herrmann, Mike Gittleson, Jon Falk, Russ Miller, John Heacock, Kevin Kolcheff, Mike Dietzel
7th Row: Brian Schrag, Dave Herrick, Jeff Tack, Pete Elezovic, Mike Maloney, Terry Looby, Brian Foster, Ron Buff, Robert Harbour, Greg Lobdell*, Joshua Wuerfel, Jim Plocki, Mike Dillon, Paul Schmidt, Bob Bland, Mike Vollmer
6th Row: Chris Stapleton, Derrick Alexander, Alfie Burch, Marc Milia, Steve Rekowski, Marc Burkholder, Eric Graves, Ninef Aghakhan, Troy Plate, Mike Lewis, Sylvester Stanley, Todd Martens, Tony McGee, Burnie Legette, Mike Nadlicki, Doug Musgrave, Dave Dobreff, Joe Barry, William Steuk
5th Row: Desmond Howard, Jon Vaughn, Dwayne Ware, Corwin Brown, Pat Maloney, Paul Manning, Randy Stark, Brian Wallace, John Albertson, Kevin Hedding, Curt Mallory, Eduardo Azcona, Doug Cohen, John Ellison, Coleman Wallace, Livetius Johnson, Dennis Washington
4th Row: Alozie Okezie*, Ra-Mon Watkins, Leon Morton, Dave Caputo, Steve Everitt, Elvis Grbac, Doug Skene, Rob Doherty, Joe Cocozzo, Martin Davis, Chris Hutchinson, Eric Knuth, Barry Kelley, Bill Schaffer, John Woodlock, Bill Madden*, Shawn Watson, Eric Traupe, Yale VanDyne
3rd Row: J.D. Carlson, Kevin Owen, Lance Dottin, Matt McCoy, Neil Simpson, Matt Elliott, Brian Townsend, Dave Diebolt, Greg Skrepenak, Alex Marshall, Dan Jokisch, Mike Evans, Dave Ritter, Ron Zielinski, Otis Williams, Steve Zacharias, Dave Knight, Chris Bohn, Eric Bush
2nd Row: Dean Dingman, Scott Smykowski, Marc Spencer, Warde Manuel, Mike Teeter, Tom Dohring, Brent White, Derrick Walker, Bobby Abrams, Greg McMurtry, J.J. Grant, Jarrod Bunch, T.J. Osman, Marc Ramirez, John Milligan, Tim Williams, Doug Daugherty, Trey Walker, Erick Anderson
Front Row: Gulam Khan, Curtis Feaster, Tony Boles, Vada Murray, David Key, Tripp Welbourne, Chris Calloway, Michael Taylor, Allen Jefferson, Leroy Hoard, Todd Plate, Rusty Fichtner, Ken Sollom
* = left the team
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1. bd. Wiegendrucke, sammelwerke, literature, literatur- und kunst-geschichte.--2. bd. Geschichte.--3. bd. Erd- und naturkunde, technik und gewerbe, philosophie, volkswirtschaft.--4. bd. Rechts- und religionskunde.
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Mode of access: Internet.
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Title also in Malayalam.
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Mode of access: Internet.
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Mode of access: Internet.
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Mode of access: Internet.
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Mode of access: Internet.
