Sistema de gestão da qualidade numa organização pública de P&D: fatores que influenciam a certificação

Pós-graduação em Engenharia Mecânica - FEG

Recuperação de papel e papelão na usina de triagem de lixo de Lençóis Paulista - SP

Pós-graduação em Ciência Florestal - FCA

Uso de aptâmeros na sexagem de sêmen bovino

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estruturação de metacomunidades de girinos em diferentes fitofisionomias da Mata Atlântica: uma análise em diferentes escalas espaciais

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Diagnóstico e proposta de diretrizes para o gerenciamento de resíduos sólidos no município de Rio Claro (SP)

Over the last few years, Brazil has some problems related to the management of solid waste, mainly related to the final disposal of materials, resulting in serious environmental risks to the municipalities that suffer from this type of situation. Poorly designed landfills and weak management systems after the termination of their employment, for example, is currently showing as a major problem for urban expansion and the subsoil of these sites, endangering all the environmental quality of the areas where they are located . Because of this scenario, this work produced a diagnosis of the current situation of the management of solid waste in the city of Rio Claro (SP) from its generation, through selective collection and sorting, as well as its final destination, in order to propose a reflection on current form management, in order to present some guidelines based on Federal Law 12.305 / 2010 - National Solid Waste Policy for improvement of this issue in the city. Although the situation found in the city can be considered reasonable for good, there is a need to improve and how to manage, especially in the operational issue of selective collection and also the participation of society in the category Environmental Education, converging in the search for improvements to the common good

Importância da segregação de materiais no gerenciamento de lixo hospitalar na área de hemoterapia

In recent years the environment and its effects on human life has been the subject of research. The scientific society organized to manage the problem of large amount of waste generated, discuss the degradation of the environment and point out possible solutions. The scarcity of references in the specific hemotherapy motivated this research. This study is an observational descriptive in order to raise the issue of hospital waste specific area of hemotherapy, presenting its latest ratings on Brazilian law also highlights the proposed Waste Management Program Health Service (PGRSS) Blood Center of Botucatu (HB) highlighting the importance of segregation of noble material in the generation of other therapeutic products using the technique of cleaner production (CP). The observational analysis of the reports shows a 20-fold increase in the amount of waste generated from 1993 to 2005, while the increase of manpower was 2.5 times. The increase in generation was due RSS hemocenter deployment of automated techniques, increased demand and improved sorting of waste. The technique implanted P + L in 2001 began using units of fresh frozen plasma, previously discarded after 12 months of storage, for the composition of new therapeutics for topical use, called bio dressing. In 10 years of implementation of this new technique, therapeutic products generated 535 patients benefited from micro-Botucatu.

Seletividade de inseticidas a alguns dos inimigos naturais na cultura do algodão

The aim of this work was study the selectivity of insecticides in favor of natural enemies in cotton (Gossypium hirsutum latifolium Hurtch Lr), DeltaOpal cultivar, in the city of Malhada (BA), and to know the associated beneficial fauna. The study was conducted at the agricultural year of 2010/2011. The design was conducted in randomized blocks with six treatments and four replications. The treatments were: (1) Fipronil 200 SC (0.38 L.ha-1); (2) Alphacypermethrin 100 SC (0.30 L.ha-1), (3) Lufenuron 50 EC (0.30 L.ha-1), (4) Imidacloprid 200 SC (0.30 L.ha-1), (5) Methyl parathion 600 EC (1.00 L.ha-1), and (6) control (water). The product was applied 80 days after emergence, and the evaluations were performed one day before application and 1, 7 and 14 days after application (DAA). The samples were taken using the sampling method beating cloth and Moericke traps. Natural enemies were brought to the laboratory for sorting, counting and identification by family. The toxicity of the products ranged according to the group of natural enemies. Imidacloprid is selective to the spiders and insecticides are moderately toxic (Methyl Parathion and Alphacypermethrin: 1 and 14th DAA; Lufenuron: 14th DAA) or toxic (Fipronil and Alphacypermethrin: 7th DAA). Fipronil (1 DAA), Alphacypermethrin (7th DAA) and Methyl  Parathion (14th DAA) are moderately toxic to adult ladybirds. The analyzed insecticides are toxic to the larvae of ladybirds, with more impact until seven days after the application, with the exception of Methyl Parathion classified, as innocuous until this period. The occurrence of 13 families of spiders and 18 families of parasitic Hymenoptera is registered in cotton agroecosystems in the region of Malhada, in the state of Bahia.

O direito de pertencer a si mesmo: a despatologização do transexualismo e sua regulamentação jurídica como direito fundamental ao gênero

Pós-graduação em Direito - FCHS

Interpretação racional do ensaio DPL

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Associations among genotype, clinical phenotype, and intracellular localization of trafficking proteins in ARC syndrome

Arthrogryposisrenal dysfunctioncholestasis (ARC) syndrome is a rare autosomal recessive multisystem disorder caused by mutations in vacuolar protein sorting 33 homologue B (VPS33B) and VPS33B interacting protein, apicalbasolateral polarity regulator (VIPAR). Cardinal features of ARC include congenital joint contractures, renal tubular dysfunction, cholestasis, severe failure to thrive, ichthyosis, and a defect in platelet alpha-granule biogenesis. Most patients with ARC do not survive past the first year of life. We report two patients presenting with a mild ARC phenotype, now 5.5 and 3.5 years old. Both patients were compound heterozygotes with the novel VPS33B donor splice-site mutation c.1225+5G>C in common. Immunoblotting and complementary DNA analysis suggest expression of a shorter VPS33B transcript, and cell-based assays show that c.1225+5G>C VPS33B mutant retains some ability to interact with VIPAR (and thus partial wild-type function). This study provides the first evidence of genotypephenotype correlation in ARC and suggests that VPS33B c.1225+5G>C mutation predicts a mild ARC phenotype. We have established an interactive online database for ARC (https://grenada.lumc.nl/LOVD2/ARC) comprising all known variants in VPS33B and VIPAR. Also included in the database are 15 novel pathogenic variants in VPS33B and five in VIPAR. Hum Mutat 33:16561664, 2012. (c) 2012 Wiley Periodicals, Inc.

Bladder expression of CD cell surface antigens and cell-type-specific transcriptomes

Many cell types have no known functional attributes. In the bladder and prostate, basal epithelial and stromal cells appear similar in cytomorphology and share several cell surface markers. Their total gene expression (transcriptome) should provide a clear measure of the extent to which they are alike functionally. Since urologic stromal cells are known to mediate organ-specific tissue formation, these cells in cancers might exhibit aberrant gene expression affecting their function. For transcriptomes, cluster designation (CD) antigens have been identified for cell sorting. The sorted cell populations can be analyzed by DNA microarrays. Various bladder cell types have unique complements of CD molecules. CD9(+) urothelial, CD104(+) basal and CD13(+) stromal cells of the lamina propria were therefore analyzed, as were CD9(+) cancer and CD13(+) cancer-associated stromal cells. The transcriptome datasets were compared by principal components analysis for relatedness between cell types; those with similarity in gene expression indicated similar function. Although bladder and prostate basal cells shared CD markers such as CD104, CD44 and CD49f, they differed in overall gene expression. Basal cells also lacked stem cell gene expression. The bladder luminal and stromal transcriptomes were distinct from their prostate counterparts. In bladder cancer, not only the urothelial but also the stromal cells showed gene expression alteration. The cancer process in both might thus involve defective stromal signaling. These cell-type transcriptomes provide a means to monitor in vitro models in which various CD-isolated cell types can be combined to study bladder differentiation and bladder tumor development based on cell-cell interaction.

Three in one: fixing marine nematodes for ecological, molecular, and morphological studies

Multidisciplinary benthic studies are still hindered by the lack of a unique fixative that satisfactorily preserves morphology and DNA, and that is simultaneously adequate for ecological surveys. The objective of this study is to test the performance of five fixatives: formalin, ethanol, dimethylsulfoxide with EDTA and NaCl salts (DESS), methanol with acetic acid (METHAC), and ethanol with acetic acid (ETHAC), for the preservation of estuarine and exclusively marine nematode assemblages for morphological, molecular, and ecological studies. The presence of the stain rose bengal in each fixative was also evaluated in the yield of PCR reactions. For molecular analyses, one species of each habitat was considered. Results revealed that fixative performance for morphological studies is habitat-and species-dependent. For studies of estuarine sediment nematodes, we recommend the use of pure ethanol, because it caused little morphological distortion (<10% of the assemblage), preserved all the species for ecological studies, and yielded high quality DNA sequences. For studies of exclusively marine environments, METHAC or DESS are the most adequate. The first performed better for morphological and ecological surveys, whereas the second was more appropriate for molecular research. For ecological studies, DESS should be used in comparison with formalin, in order to cross check the results. Finally, staining of samples with rose bengal is not recommended, because it hindered DNA amplification regardless of the fixative used.

Juvenile myoclonic epilepsy: The impact of clinical variables and psychiatric disorders on executive profile assessed with a comprehensive neuropsychological battery

Executive dysfunction is reported in juvenile myoclonic epilepsy (JME). However, batteries employed in previous studies included no more than three tests of executive function. In this study, we aimed to assess executive and attentional functions in JME using a comprehensive battery of eight tests (encompassing fifteen subtests). We also evaluated neuropsychological profiles using a clinical criterion of severity and correlated these findings with epilepsy clinical variables and the presence of psychiatric disorders. We prospectively evaluated 42 patients with JME and a matched control group with Digit Span tests (forward and backward), Stroop Color-Word Test, Trail Making Test, Wisconsin Card-Sorting Test, Matching Familiar Figures Test and Word Fluency Test. We estimated IQ with the Matrix Reasoning and Vocabulary subtests of the Wechsler Abbreviated Intelligence Scale. The patients with JME showed specific deficits in working memory, inhibitory control, concept formation, goal maintenance, mental flexibility, and verbal fluency. We observed attentional deficits in processes such as alertness and attention span and those requiring sustained and divided attention. We found that 83.33% of the patients had moderate or severe executive dysfunction. In addition, attentional and executive impairment was correlated with higher frequency of seizures and the presence of psychiatric disorders. Furthermore, executive dysfunction correlated with a longer duration of epilepsy. Our findings indicate the need for comprehensive neuropsychological batteries in patients with JME, in order to provide a more extensive evaluation of attentional and executive functions and to show that some relevant deficits have been overlooked. (C) 2012 Elsevier Inc. All rights reserved.

Speech Graphs Provide a Quantitative Measure of Thought Disorder in Psychosis

Background: Psychosis has various causes, including mania and schizophrenia. Since the differential diagnosis of psychosis is exclusively based on subjective assessments of oral interviews with patients, an objective quantification of the speech disturbances that characterize mania and schizophrenia is in order. In principle, such quantification could be achieved by the analysis of speech graphs. A graph represents a network with nodes connected by edges; in speech graphs, nodes correspond to words and edges correspond to semantic and grammatical relationships. Methodology/Principal Findings: To quantify speech differences related to psychosis, interviews with schizophrenics, manics and normal subjects were recorded and represented as graphs. Manics scored significantly higher than schizophrenics in ten graph measures. Psychopathological symptoms such as logorrhea, poor speech, and flight of thoughts were grasped by the analysis even when verbosity differences were discounted. Binary classifiers based on speech graph measures sorted schizophrenics from manics with up to 93.8% of sensitivity and 93.7% of specificity. In contrast, sorting based on the scores of two standard psychiatric scales (BPRS and PANSS) reached only 62.5% of sensitivity and specificity. Conclusions/Significance: The results demonstrate that alterations of the thought process manifested in the speech of psychotic patients can be objectively measured using graph-theoretical tools, developed to capture specific features of the normal and dysfunctional flow of thought, such as divergence and recurrence. The quantitative analysis of speech graphs is not redundant with standard psychometric scales but rather complementary, as it yields a very accurate sorting of schizophrenics and manics. Overall, the results point to automated psychiatric diagnosis based not on what is said, but on how it is said.

Constraining anomalous Higgs boson interactions

The recently announced Higgs boson discovery marks the dawn of the direct probing of the electroweak symmetry breaking sector. Sorting out the dynamics responsible for electroweak symmetry breaking now requires probing the Higgs boson interactions and searching for additional states connected to this sector. In this work, we analyze the constraints on Higgs boson couplings to the standard model gauge bosons using the available data from Tevatron and LHC. We work in a model-independent framework expressing the departure of the Higgs boson couplings to gauge bosons by dimension-six operators. This allows for independent modifications of its couplings to gluons, photons, and weak gauge bosons while still preserving the Standard Model (SM) gauge invariance. Our results indicate that best overall agreement with data is obtained if the cross section of Higgs boson production via gluon fusion is suppressed with respect to its SM value and the Higgs boson branching ratio into two photons is enhanced, while keeping the production and decays associated to couplings to weak gauge bosons close to their SM prediction.