The conserved SOCS box motif in suppressors of cytokine signaling binds to elongins B and C and may couple bound proteins to proteasomal degradation

The suppressors of cytokine signaling (SOCS) family of proteins act as intracellular inhibitors of several cytokine signal transduction pathways. Their expression is induced by cytokine activation of the Janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway and they act as a negative feedback loop by subsequently inhibiting the JAK/STAT pathway either by direct interaction with activated JAKs or with the receptors. These interactions are mediated at least in part by the SH2 domain of SOCS proteins but these proteins also contain a highly conserved C-terminal homology domain termed the SOCS box. Here we show that the SOCS box mediates interactions with elongins B and C, which in turn may couple SOCS proteins and their substrates to the proteasomal protein degradation pathway. Analogous to the family of F-box-containing proteins, it appears that the SOCS proteins may act as adaptor molecules that target activated cell signaling proteins to the protein degradation pathway.

Crystal structure of the C-terminal domain of the RAP74 subunit of human transcription factor IIF

The x-ray structure of a C-terminal fragment of the RAP74 subunit of human transcription factor (TF) IIF has been determined at 1.02-Å resolution. The α/β structure is strikingly similar to the globular domain of linker histone H5 and the DNA-binding domain of hepatocyte nuclear factor 3γ (HNF-3γ), making it a winged-helix protein. The surface electrostatic properties of this compact domain differ significantly from those of bona fide winged-helix transcription factors (HNF-3γ and RFX1) and from the winged-helix domains found within the RAP30 subunit of TFIIF and the β subunit of TFIIE. RAP74 has been shown to interact with the TFIIF-associated C-terminal domain phosphatase FCP1, and a putative phosphatase binding site has been identified within the RAP74 winged-helix domain.

X-ray structure of the human hyperplastic discs protein: An ortholog of the C-terminal domain of poly(A)-binding protein

The poly(A)-binding protein (PABP) recognizes the 3′ mRNA poly(A) tail and plays an essential role in eukaryotic translation initiation and mRNA stabilization/degradation. PABP is a modular protein, with four N-terminal RNA-binding domains and an extensive C terminus. The C-terminal region of PABP is essential for normal growth in yeast and has been implicated in mediating PABP homo-oligomerization and protein–protein interactions. A small, proteolytically stable, highly conserved domain has been identified within this C-terminal segment. Remarkably, this domain is also present in the hyperplastic discs protein (HYD) family of ubiquitin ligases. To better understand the function of this conserved region, an x-ray structure of the PABP-like segment of the human HYD protein has been determined at 1.04-Å resolution. The conserved domain adopts a novel fold resembling a right-handed supercoil of four α-helices. Sequence profile searches and comparative protein structure modeling identified a small ORF from the Arabidopsis thaliana genome that encodes a structurally similar but distantly related PABP/HYD domain. Phylogenetic analysis of the experimentally determined (HYD) and homology modeled (PABP) protein surfaces revealed a conserved feature that may be responsible for binding to a PABP interacting protein, Paip1, and other shared interaction partners.

An evaluation of the 1979 Texas law (V.C.S., Sec. 73.A.) which requires frontage road traffic to yield at freeway ramps. Interim report.

Texas State Department of Highways and Public Transportation, Transportation Planning Division, Austin

National security assessment of the U.S. shipbuilding and repair industry / prepared by Stephen Baker ... [et al.].

"May 2001."

Six anonymous plays. First series (c. 1510-1537) comprising Four elements--The beauty and good properties of women (usually known as Calisto and Melibæa)--Every man--Hickscorner--The world and the child--Thersites--Note-book and word-list, ed.

With facsimile reproductions of original title-pages.

The golden horseshoe; extracts from the letters of Captain H. L. Herndon of the 21st U.S. infantry, on duty in the Philippine Islands, adn Lieutenant Lawrence Gill, A.D.C. to the military governor of Puerto Rico.

Mode of access: Internet.

Sketches of the life and times of the Rev. Stephen Bliss, A.M. : with notices of his co-laborers, Rev. Isaac Bennet [and others] /

Mode of access: Internet.

The life and character of Stephen Decatur; late commodore and post-captain in the navy of the United States, and navy-commissioner: interspersed with brief notices of the origin, progress, and achievements of the American navy.

"The miniature portraits' of Bainbridge, Porter, Lawrence, and Macdonald, were furnished by another hand." cf. Pref.

An introduction to a general system of hydrostaticks and hydraulicks, philosophical and practical. Wherein the most reasonable and advantageous methods of raising and conducting water, for the watering noblemens and gentlemens seats, buildings, gardens, &c., are carefully (and in a manner not yet publish'd in any language) laid down ... Illustrated and explain'd by sixty copper cuts, done by the best hands ... Collected from the best of the Italian and French designs (together with some new ones of the author's own invention) ...

Paged continuously.

More thoughts occasioned by two publications : which the authors call An exposure of some of the numerous misstatements and misrepresentations contained in a pamphlet commonly known by the name of Mr. Marryat's pamphlet, entitled Thoughts, &c., and, A defence of the bill for the registration of slaves /

Includes bibliographical references.

A treatise on the French verbs, regular, irregular, defective, &c. with notes and illustrations, selected from lthe most approved grammarians.

Mode of access: Internet.

The memoirs of Charles-Lewis, Baron de Pollnitz. Being the observations he made in his late travels from Prussia thro' Germany, Italy, France, Flanders, Holland, England, &c. In letters to his friend, discovering not only the present state of the chief cities and towns; but the characters of the principal persons at the several courts.

"Account of the electoral family of Saxony, translated from the Bron's State of it": v. 1, p. 99-155.

Modeling southern pine beetle populations, symposium proceedings : Asheville, N.C., February 20-22, 1980 /

"September 1980."