Use of reverse transcriptase polymerase chain reaction (RT-PCR) in molecular screening of Newcastle disease virus in poultry and free-living bird populations

The aim of this study was to evaluate a simple molecular method of reverse transcriptase polymerase chain reaction (RT-PCR) to differentiate Newcastle disease virus strains according to their pathogenicity, in order to use it in molecular screening of Newcastle disease virus in poultry and free-living bird populations. Specific primers were developed to differentiate LaSota-LS-(vaccine strain) and Sao Joao do Meriti-SJM-strain (highly pathogenic strain). Chickens and pigeons were experimentally vaccinated/infected for an in vivo study to determine virus shedding in feces. Validation of sensitivity and specificity of the primers (SJM and LS) by experimental models used in the present study and results obtained in the molecular analysis of the primers by BLAST made it possible to generalize results. The development of primers that differentiate the level of pathogenicity of NDV stains is very important, mainly in countries where real-time RT-PCR is still not used as a routine test. These primers were able to determine the presence of the agent and to differentiate it according to its pathogenicity. © 2012 Springer Science+Business Media B.V.

Migraciones y salud en el complejo siderúrgico-petroquímico de Cubatao, Sao Paulo

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Compartimentação geomorfológica e processos deposicionais no megaleque fluvial do rio São Lourenço, Pantanal mato-grossense

The Pantanal wetland is an extensive depositional tract characterized by the presence of fluvial megafans, from which that of the Taquari River is the most extensive and well known. Located on the northern border of the Pantanal, the São Lourenço megafan is a large distributary fluvial system with an area of 16,000 km2, but poorly known regarding its geomorphological and geological features. The Sao Lourenço and the Taquari are coalescent megafans, having the Piquiri interfan meander river in between them. Based on the interpretation of satellite images and field validation, it was possible to identify channel patterns, to interpret depositional and erosional processes, to recognize the phenomena of river avulsion and to map depositional lobes. Three geomorphological zones were recognized on the São Lourenço megafan: 1) abandoned depositional lobes located in the upper/intermediate fan, composed of Pleistocene fluvial deposits and exhibiting distributary paleochannels on their surfaces, which are currently being dissected by tributary channels; 2) an active Holocene confined meander belt formed by fluvial aggradation in a N65E incised-valley; 3) active depositional lobes placed on the distal part of the system, which are the main site of sedimentation and are characterized by frequent avulsion events, channel bifurcation, distributary drainage pattern and the presence of depositional lobated landforms. The active depositional lobes were formed due to an important event of river avulsion that caused the lower portion of the meander belt to be abandoned.

Local and regional features of surface radiation fluxes over the tropical Atlantic ocean near Sao Pedro and Sao Paulo archipelago: Evidence of small scale upwelling

To properly describe the interactions between the ocean and atmosphere, it is necessary to assess a variety of time and spatial scales phenomena. Here, high resolution oceanographic and meteorological data collected during an observational campaign carried out aboard a ship in the tropical Atlantic Ocean, on May 15-24, 2002, is used to describe the radiation balance at the ocean interface. Data collected by two PIRATA buoys, along the equator at 23°W and 35°W and satellite and climate data are compared with the data obtained during the observational campaign. Comparison indicates remarkable similarity for daily and hourly values of radiation fluxes components as consequence of the temporal and spatial consistence presented by the air and water temperatures measured in situ and estimated from large scale information. The discrepancy, mainly in the Sao Pedro and Sao Paulo Archipelago area, seems to be associated to the local upwelling of cold water, which is not detected in all other estimates investigated here. More in situ data are necessary to clarify whether this upwelling flow has a larger scale effect and what are the meteorological and oceanographic implications of the local upwelling area on the tropical waters at the Brazilian coast.

La industria del café en Sao Paulo = The coffee industry in Sao Paulo

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Caracterização química e mineralógica de azulejos portugueses do centro histórico de São Luís-MA (Brasil): Uma aproximação à mineralogia e à temperatura de queima das matérias-primas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Discontinuidades demográficas en Brasil y el Estado de Sao Paulo

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Efectos sociales de la rápida industrialización: el caso de Sao José dos Campos

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Condición migratoria, raza y género en el mercado de trabajo brasileño: el caso de las regiones metropolitanas de Río de Janeiro y Sao Paulo

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Occurrence of mycobacteria in bovine milk samples from both individual and collective bulk tanks at farms and informal markets in the southeast region of Sao Paulo, Brazil

Background: Mycobacterium spp. is one of the most important species of zoonotic pathogens that can be transmitted from cattle to humans. The presence of these opportunistic, pathogenic bacteria in bovine milk has emerged as a public-health concern, especially among individuals who consume raw milk and related dairy products. To address this concern, the Brazilian control and eradication program focusing on bovine tuberculosis, was established in 2001. However, bovine tuberculosis continues to afflict approximately 1,3 percent of the cattle in Brazil. In the present study, 300 samples of milk from bovine herds, obtained from both individual and collective bulk tanks and informal points of sale, were cultured on Löwenstein-Jensen and Stonebrink media. Polymerase chain reaction (PCR)-based tests and restriction-enzyme pattern analysis were then performed on the colonies exhibiting phenotypes suggestive of Mycobacterium spp., which were characterized as acid-fast bacilli.Results: Of the 300 bovine milk samples that were processed, 24 were positively identified as Mycobacterium spp.Molecular identification detected 15 unique mycobacterial species: Mycobacterium bovis, M. gordonae, M. fortuitum, M. intracellulare, M. flavescens, M. duvalii, M. haemophilum, M. immunogenum, M. lentiflavum, M. mucogenicum, M. novocastrense, M. parafortuitum, M. smegmatis, M. terrae and M. vaccae. The isolation of bacteria from the various locations occurred in the following proportions: 9 percent of the individual bulk-tank samples, 7 percent of the collective bulk-tank samples and 8 percent of the informal-trade samples. No statistically significant difference was observed between the presence of Mycobacterium spp. in the three types of samples collected, the milk production profiles, the presence of veterinary assistance and the reported concerns about bovine tuberculosis prevention in the herds.Conclusion: The microbiological cultures associated with PCR-based identification tests are possible tools for the investigation of the presence of Mycobacterium spp. in milk samples. Using these methods, we found that the Brazilian population may be regularly exposed to mycobacteria by consuming raw bovine milk and related dairy products. These evidences reinforces the need to optimize quality programs of dairy products, to intensify the sanitary inspection of these products and the necessity of further studies on the presence of Mycobacterium spp. in milk and milk-based products. © 2013 Franco et al.; licensee BioMed Central Ltd.

Carta de Valdés Rodríguez de la Delegación de Cuba al Dr. Luis machado y Ortega Presidente de la II Sesión de la Comisión Económica para América Latina

Solicita la inclusión en la Agenda de la Conferencia del tema "Turismo"

Letter of Valdés Rodriguez President of the Cuban Delegation to Dr. Luis Machado y Ortega President of the Second Session of the Economic Commission for Latin America

Request the inclusion into the Agenda of the Conference of the item "Turismo"

Carta de Alberto Baltra C. Presidente de la Delegación de Chile al Dr. Luis Machado, Presidente de la Comisión Económica para América Latina

Sugiere introducir algunas modificaciones al Reglamento para lograr una mayor eficiencia en la labor de la Comisión