Acute leukemia in early childhood

Acute leukemia in early childhood is biologically and clinically distinct. The particular characteristics of this malignancy diagnosed during the first months of life have provided remarkable insights into the etiology of the disease. The pro-B, CD10 negative immunophenotype is typically found in infant acute leukemia, and the most common genetic alterations are the rearrangements of the MLL gene. In addition, the TEL/AML1 fusion gene is most frequently found in children older than 24 months. A molecular study on a Brazilian cohort (age range 0-23 months) has detected TEL/AML1+ve (N = 9), E2A/PBX1+ve (N = 4), PML/RARA+ve (N = 4), and AML1/ETO+ve (N = 2) cases. Undoubtedly, the great majority of genetic events occurring in these patients arise prenatally. The environmental exposure to damaging agents that give rise to genetic changes prenatally may be accurately determined in infants since the window of exposure is limited and known. Several studies have shown maternal exposures that may give rise to leukemogenic changes. The Brazilian Collaborative Study Group of Infant Acute Leukemia has found that mothers exposed to dipyrone, pesticides and hormones had an increased chance to give birth to babies with infant acute leukemia [OR = 1.48 (95%CI = 1.05-2.07), OR = 2.27 (95%CI = 1.56-3.31) and OR = 9.08 (95%CI = 2.95-27.96)], respectively. This review aims to summarize recent clues that have facilitated the elucidation of the biology of early childhood leukemias, with emphasis on infant acute leukemia in the Brazilian population.

Argumentum quoddam a priori, pro demonstranda Dei existentia, exhibens

Invokaatio: D.F.G.

Prokaryotic expression of a novel mouse pro-apoptosis protein PNAS-4 and application of its polyclonal antibodies

Mouse PNAS-4 (mPNAS-4) has 96% identity with human PNAS-4 (hPNAS-4) in primary sequence and has been reported to be involved in the apoptotic response to DNA damage. However, there have been no studies reported of the biological functions of mPNAS-4. In studies conducted by our group (unpublished data), it was interesting to note that overexpression of mPNAS-4 promoted apoptotic death in Lewis lung carcinoma cells (LL2) and colon carcinoma cells (CT26) of mice both in vitro and in vivo. In our studies, mPNAS-4 was cloned into the pGEX-6P-1 vector with GST tag at N-terminal in Escherichia coli strain BL21(DE3). The soluble and insoluble expression of recombinant protein mPNAS-4 (rmPNAS-4) was temperature-dependent. The majority of rmPNAS-4 was insoluble at 37°C, while it was almost exclusively expressed in soluble form at 20°C. The soluble rmPNAS-4 was purified by one-step affinity purification, using a glutathione Sepharose 4B column. The rmPNAS-4 protein was further identified by electrospray ionization-mass spectrometry analysis. The search parameters of the parent and fragment mass error tolerance were set at 0.1 and 0.05 kDa, respectively, and the sequence coverage of search result was 28%. The purified rmPNAS-4 was further used as immunogen to raise polyclonal antibodies in New Zealand white rabbit, which were suitable to detect both the recombinant and the endogenous mPNAS-4 in mouse brain tissue and LL2 cells after immunoblotting and/or immunostaining. The purified rmPNAS-4 and our prepared anti-mPNAS-4 polyclonal antibodies may provide useful tools for future biological function studies for mPNAS.

De argumento pro existentia Summi Numinis physico-theologico

Arkit: 1 arkintunnukseton lehti, A-B4 C2.

Casus vulneris cum fractura olecrani et ruptura ancyloseos verae complicati; quem venia exper. fac. med. praeside Joh. Agap. Törngren, m. d. chir. [et] art. obst. professore p. [et] o. Pro gradu medico publicae submittit censurae Andreas Jacobus Hjertman, stip. publ. Obstrob. [!] In audit. med. die XXVI Octob. MDCCCXVI, h. a. & p. m. s

Dedikaatio: Carolus Mannerheim.

De modo reducendi distantias lunae a stellis pro longitudine geographica invenienda 2

Arkit: 1 arkintunnukseton lehti, C3-C4 D-E4. - S. [2] tyhjä.

De modo reducendi distantias lunae a stellis pro longitudine geographica invenienda 1

Arkit: 1 arkintunnukseton lehti, A-B4 C2. - S. [2] tyhjä.

Comparatio diversorum experimentorum ad definiendam densitatem et volumen aquae pro diversa caloris temperie 1

Arkit: 1 arkintunnukseton lehti, A4 B2. - S. [2] tyhjä.

Effect of TNF-α production inhibitors on the production of pro-inflammatory cytokines by peripheral blood mononuclear cells from HTLV-1-infected individuals

Human T lymphotropic virus type 1 (HTLV-1) is the causal agent of myelopathy/tropical spastic paraparesis (HAM/TSP), a disease mediated by the immune response. HTLV-1 induces a spontaneous proliferation and production of pro-inflammatory cytokines by T cells, and increasing interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) levels are potentially involved in tissue damage in diseases related to HTLV-1. This exaggerated immune response is also due to an inability of the natural regulatory mechanisms to down-modulate the immune response in this group of patients. TNF-α inhibitors reduce inflammation and have been shown to improve chronic inflammatory diseases in clinical trials. The aim of this study was to evaluate the ability of pentoxifylline, forskolin, rolipram, and thalidomide to decrease in vitro production of TNF-α and IFN-γ in cells of HTLV-1-infected subjects. Participants of the study included 19 patients with HAM/TSP (mean age, 53 ± 11; male:female ratio, 1:1) and 18 HTLV-1 carriers (mean age, 47 ± 11; male:female ratio, 1:2.6). Cytokines were determined by ELISA in supernatants of mononuclear cell cultures. Pentoxifylline inhibited TNF-α and IFN-γ synthesis with the minimum dose used (50 µM). The results with forskolin were similar to those observed with pentoxifylline. The doses of rolipram used were 0.01-1 µM and the best inhibition of TNF-α production was achieved with 1 µM and for IFN-γ production it was 0.01 µM. The minimum dose of thalidomide used (1 µM) inhibited TNF-α production but thalidomide did not inhibit IFN-γ production even when the maximum dose (50 µM) was used. All drugs had an in vitro inhibitory effect on TNF-α production and, with the exception of thalidomide, all of them also decreased IFN-γ production.

De interpolatione pro inveniendo loco lunae ex ephemeridibus

Arkit: A4 B2. - S. [2] tyhjä.

Disputatio pro articulo VII. Libri concordiae

Variantti B.

Disputatio pro articulo I. Libri concordiae

Variantti A.

Spicilegium plantarum cryptogamarum Sveciae, pro argumento publicae disputationis, in Regiae Academiae Aboënsis aud. maj. venia experientissimae facultatis medicae, d. XVIII Dec. h. a. MDCCXCIV, horis solitis agendae, propositum, vindicibus auctore Carolo Birgero Rutström, ph. & m. d. med. adj. & botan. demonstr. et Johanne Gustavo Haartman, stipendiario Haartmanniano natione Australi

Dedikaatio: Carolus Adamus Wachtmeister.

Positiones historico-politicae e Chronico episcoporum m.scr. Pauli Justen. excerptae, quas consentiente [et] approbante ampliss. facult. philosoph. Acad. Aboensis, praeside viro cl. mag. Algotho A. Scarin histor. & polit profess. ord. Pro obtinendis honoribus philosophicis in auditorio supremo & maximo ad diem IX Jun. MDCCXXVI. Publice examinandas sistit Israel Escholin v. pastor in Sagu

Invokaatio: Q.B.V.

Dissertatio academica Davidem regem hypnopsychitis non favere, demonstrans, contra Heinium ex occasione psalm. VI. Quam, consentiente ampliss. facult. philosoph. in regio ad Auram lycaeo, suffultus praesidio viri maxime reverendi atque celeberrimi d:ni Isaaci Ross, s. s. l. l. profess. reg. & ord. fac. phil. h. t. decani maxime spectabilis pro consequendis honoribus academicis publico examini modeste submittit Arvid Favorin, Nic. fil. Satacundensis, in aud. majori die XXII. Decembr. anni MDCCLIX. h. a. m. s

Invokaatio: J.M.G.