Mecanismos da infertilidade causada por Gossipol em ratos e efeito protetor da vitamina E

Pós-graduação em Ciência e Tecnologia Animal - FEIS

Balanced anesthesia with sevoflurane does not alter redox status in patients undergoing surgical procedures

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Structural characterization of an etheno-2 '-deoxyguanosine adduct modified by tetrahydrofuran

The reaction of 2'-deoxyguanosine with the alpha,beta-unsaturated aldehydes trans-2-octenal, trans-2-nonenal, trans-2-decenal, trans,trans-2,4-nonadienal, and trans,trans-2,4-decadienal in THF gives rise to three novel adducts: 3-(2'-deoxy-beta-D-erythro-pentafuranosyl)-7-[3-hydroxy-1-(3(2'-deoxy-beta-D-erythro-pentafuranosyl)-3,5-dihydro-imidazo[1,2-alpha]purin-9-one-7-yl)-propyl] -3,5-dihydro-imidazo[1,2-alpha]purin-9-one (M) and 3-(2'-deoxy-beta-D-erythro-pentafuranosyl)-7-(tetrahydrofuran-2-yl)-3,5-dihydro-imidazo[1,2-alpha]purin-9-one (A8 and A9), which are not observed in the absence of THF. These adducts were isolated from in vitro reactions by reversed-phase HPLC and fully characterized on the basis of spectroscopic measurements. Adduct A7 consists of two 1,N-2-etheno-2'-deoxyguanosine (1,N-2-epsilondGuo) residues linked to a hydroxy-carbon side chain; adducts A8 and A9 are interconvertible 1,N-2-epsilondGuo derivatives bearing a THF moiety. The proposed reaction mechanism involves the electrophilic attack on 1,N-2-epsilondGuo by the carbonyl of 4-hydroxy-butanal, generated via ring opening of alpha-hydroxy-THF (THF-OH), yielding adducts A8 and A9. A further combination of these adducts with another 1,N-2-epsilondGuo produces the double adduct A7. These findings demonstrate that reactions of unsaturated aldehydes in the presence of THF produce novel condensation 1,N-2-epsilondGuo-THF adducts. Further studies would indicate the relevance of these adducts in THF toxicity.

Oxidative Stress in Maternal Blood and Placenta From Mild Diabetic Rats

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

L-Carnitine supplementation impairs endothelium-dependent relaxation in mesenteric arteries from rats

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Antioxidantes e biomarcadores da injúria oxidativa na saliva de pacientes com síndrome de down

Vulnerability of patients with Down syndrome (DS) to oxidative stress and damage has been attributed to the overexpression of the superoxide dismutase gene, which is located in the triplicated critical region 21q22.2 of chromosome 21. The objective of this study was to investigate enzymatic and non-enzymatic antioxidant systems and levels of biomarkers of oxidative damage in saliva of patients with DS. Saliva samples were collected from 30 patients with DS and 30 controls, ranging in age from 14 to 24 years. The following parameters were analyzed: superoxide dismutase activity, concentration of malondialdehyde, carbonylated proteins, uric acid, vitamin C and total protein, peroxidase activity, and total antioxidant capacity. Patients with DS presented significantly higher superoxide dismutase activity and malondialdehyde levels than controls (p<0.05). On the other hand, no difference in carbonylated proteins or antioxidants (uric acid, vitamin C, peroxidase, and total antioxidant capacity) was observed between DS patients and controls (p>0.05). Patients with DS also presented higher salivary total protein content (p<0.05). In conclusion, despite similar antioxidant levels patients with DS are more vulnerable to oxidative stress in saliva as indicated by a significant increase in malondialdehyde concentration and superoxide dismutase activity

Estudo de variações bioquímicas e genotoxicidade induzidas por mistura de contaminantes em tilápia (Oreochromis niloticus), como biomarcadores de contaminação ambiental

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Potential utility of melatonin as an antioxidant therapy in the management of sickle cell anemia

This study aimed to assess antioxidant effects of melatonintreatment compared to N-acetylcysteine (NAC) and to their combination in asickle cell suspension. Sickle erythrocytes were suspended in phosphate-buffered saline, pH 7.4, composing external control group. They were alsosuspended and incubated at 37°C either in the absence (experimental controlgroup) or in the presence of NAC, melatonin and their combination atconcentrations of 100 pM, 100 nM and 100 lM for 1 hr (treatment groups).The melatonin influences were evaluated by spectrophotometric [hemolysisdegree, catalase (CAT), glutathione S-transferase (GST), glutathioneperoxidase (GPx), glutathione reductase (GR), glucose-6-phosphatedehydrogenase (G6PDH), and superoxide dismutase (SOD) activities] andchromatographic methods [glutathione (GSH) and malondialdehyde (MDA)levels]. Incubation period was able to cause a rise about 64% on hemolysisdegree as well as practically doubled the lipid peroxidation levels (P < 0.01).However, almost all antioxidants tested treatments neutralized this incubationeffect observed in MDA levels. Among the antioxidant biomarkers evaluated,we observed a modulating effect of combined treatment on GPx and SODactivities (P < 0.01), which showed ~25% decrease in their activities. Inaddition, we found an antioxidant dose-dependent effect for melatonin onlipid peroxidation (r = 0.29; P = 0.03) and for combined antioxidanttreatments also on MDA levels (r = 0.37; P = 0.01) and on SOD activity(r = 0.54; P < 0.01). Hence, these findings contribute with important insightthat melatonin individually or in combination with NAC may be useful forsickle cell anemia management.

Avaliação de danos oxidativos no DNA de linfócitos e estresse oxidativo no plasma de pacientes a cirurgia sob anestesia balanceada com sevoflurano

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Metabolismo oxidativo sérico em ratos diabéticos com lesão periapical

Pós-graduação em Ciência Odontólogica - FOA

Alterações em marcadores do estresse oxidativo ocasionadas por diferentes tempos de tratamento com chá mate (Ilex paraguariensis) no processo de envelhecimento

Pós-graduação em Ciências fisiológicas

Catalisadores de cobre suportados em óxidos de zircônio hierarquicamente estruturados para a reação de desidrogenação de etanol

Pós-graduação em Química - IQ

Respostas bioquímicas e fisiológicas de plantas jovens de cana-de-açúcar sob diferentes concentrações de NaCl no solo

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

Effect of protective solutions and hydroxyethyl starch in the attenuation of the injuries of ischemia and reperfusion of splanchnic organs

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeito da suplementação com vitamina E sobre os metabolismos oxidativo e cardíaco em equinos submetidos a exercício de alta intensidade

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)