Structure-Based Mutational Analysis of eIF4E in Relation to sbm1 Resistance to Pea Seed-Borne Mosaic Virus in Pea

Background: Pea encodes eukaryotic translation initiation factor eIF4E (eIF4E(S)), which supports the multiplication of Pea seed-borne mosaic virus (PSbMV). In common with hosts for other potyviruses, some pea lines contain a recessive allele (sbm1) encoding a mutant eIF4E (eIF4E(R)) that fails to interact functionally with the PSbMV avirulence protein, VPg, giving genetic resistance to infection.

Created relation: the translated play in performance

Summary: The process of translation restores the materiality of time and space to a play. Performance, of course, takes place only within the here and now of an audience, but a play that comes from a different time or different place prompts an encounter with the conceptual and perceptual real from sometime or somewhere else. If we are to consider translation as an ethical practice, one concerned not only to present the dislocated other to the located self, but also to protect the other from wholesale assimilation by the self, then the translated play must in some ways prove resistant to such assimilation. But of course the retention of foreignness – or what Steiner called restitution – is problematic, not least because of the attendant danger of merely exoticising. The theatre of García Lorca in English presents an interesting case study in this regard.

Apparatus and method for adjusting filter frequency in relation to sampling frequency

A self-tuning filter is disclosed. The self-tuning filter includes a digital clocking signal and an input coupled to the digital clocking signal, whereby the input reads a value incident on the input when the digital clocking signal changes to a predetermined state. A clock-tunable filter is, furthermore, coupled to the digital clocking signal so that the frequency of the clock-tunable filter is adjusted in relation to a sampling frequency at which the digital clocking signal operates. The self-tuning filter may be applied to an input of a data acquisition unit and applied to an input having a variable sampling frequency. A method of controlling the frequency of a clock-tunable filter is also disclosed.

In vitro resistance of Burkholderia cepacia complex isolates to reactive oxygen species in relation to catalase and superoxide dismutase production

The Burkholderia cepacia complex comprises groups of genomovars (genotypically distinct strains with very similar phenotypes) that have emerged as important opportunistic pathogens in cystic fibrosis (CF) patients. The inflammatory response against bacteria in the airways of CF individuals is dominated by polymorphonuclear cells and involves the generation of oxidative stress, which leads to further inflammation and tissue damage. Bacterial catalase, catalase-peroxidase and superoxide dismutase activities may contribute to the survival of B. cepacia following exposure to reactive oxygen metabolites generated by host cells in response to infection. In the present study the authors investigated the production of catalase, peroxidase and SOD by isolates belonging to various genomovars of the B. cepacia complex. Production of both catalase and SOD was maximal during late stationary phase in almost all isolates examined. Native PAGE identified 13 catalase electrophoretotypes and two SOD electrophoretotypes (corresponding to an Fe-SOD class) in strains belonging to the six genomovars of the B. cepacia complex. Seven out of 11 strains displaying high-level survival after H(2)O(2) treatment in vitro had a bifunctional catalase/peroxidase, and included all the genomovar III strains examined. These isolates represent most of the epidemic isolates that are often associated with the cepacia syndrome. The majority of the isolates from all the genomovars were resistant to extracellular O(-)(2), while resistance to intracellularly generated O(-)(2)was highly variable and could not be correlated with the detected levels of SOD activity. Altogether the results suggest that resistance to toxic oxygen metabolites from extracellular sources may be a factor involved in the persistence of B. cepacia in the airways of CF individuals.

New generalized dispersion relation for low-frequency electromagnetic waves in Hall-magnetohydrodynamic dusty plasma

A generalized linear theory for electromagnetic waves in a homogeneous dusty magnetoplasma is presented. The waves described are characterized by a frequency which is much smaller (larger) than the electron gyrofrequency (dust plasma and dust gyrofrequencies), and a long wavelength (in comparison with the ion gyroradius and the electron skin depth). The generalized Hall- magnetohydrodynamic (GH-MHD) equations are derived by assuming massive charged dust macroparticles to be immobile, and Fourier transformed to obtain a general dispersion relation. The latter is analyzed to understand the influence of immobile charged dust grains on various electromagnetic wave modes in a magnetized dusty plasma.