Audit report on the Office of Treasurer of State, Iowa Educational Savings Plan Trust for the year ended June 30, 2011

Audit report on the Office of Treasurer of State, Iowa Educational Savings Plan Trust for the year ended June 30, 2011

Audit report on the Rathbun Area Solid Waste Commission for the year ended June 30, 2011

Audit report on the Rathbun Area Solid Waste Commission for the year ended June 30, 2011

Audit report on the Iowa Agricultural Development Authority for the year ended June 30, 2011

Audit report on the Iowa Agricultural Development Authority for the year ended June 30, 2011

Audit report on the Iowa Judicial Retirement System for the year ended June 30, 2011

Audit report on the Iowa Judicial Retirement System for the year ended June 30, 2011

Audit report on the Iowa Corn Promotion Board for the years ended August 31, 2011 and 2010

Audit report on the Iowa Corn Promotion Board for the years ended August 31, 2011 and 2010

Audit report on Iowa Public Television for the year ended June 30, 2011

Audit report on Iowa Public Television for the year ended June 30, 2011

Audit report on the Council Bluffs Airport Authority for the year ended June 30, 2011

Audit report on the Council Bluffs Airport Authority for the year ended June 30, 2011

Audit report on the Iowa Lottery Authority for the year ended June 30, 2011

Audit report on the Iowa Lottery Authority for the year ended June 30, 2011

Audit report on the Honey Creek Resort Operations Account maintained by Central Group Management, LLC for the year ended June 30, 2011

Audit report on the Honey Creek Resort Operations Account maintained by Central Group Management, LLC for the year ended June 30, 2011

Audit report on the Peace Officers' Retirement, Accident and Disability System for the year ended June 30, 2011

Audit report on the Peace Officers' Retirement, Accident and Disability System for the year ended June 30, 2011

Audit report on Internal Control over Financial Reporting of the State University of Iowa as of and for the year ended June 30, 2011

Audit report on Internal Control over Financial Reporting of the State University of Iowa as of and for the year ended June 30, 2011

Audit report on the Central Iowa Juvenile Detention Center in Eldora, Iowa for the year ended June 30, 2011

Audit report on the Central Iowa Juvenile Detention Center in Eldora, Iowa for the year ended June 30, 2011

Audit report on the Great River Regional Waste Authority for the year ended June 30, 2011

Audit report on the Great River Regional Waste Authority for the year ended June 30, 2011

Pigs Transgenic For Human Dominant Mutations Of The GUCY2D Gene

Purpose: Studies on large animal models are an important step to test new therapeutical strategies before human application. Considering the importance of cone function for human vision and the paucity of large animal models for cone dystrophies having an enriched cone region, we propose to develop a pig model for cone degeneration. With a lentiviral-directed transgenesis, we obtained pigs transgenic for a cone-dominant mutant gene described in a human cone dystrophy.Methods: Lentiviral vectors encoding the human double mutant GUCY2DE837D/R838S cDNA under the control of a region of the pig arrestin-3 promoter (Arr3) was produced and used for lentiviral-derived transgenesis in pigs. PCR-genotyping and southern blotting determined the genotype of pigs born after injection of the vector at the zygote stage. Retina function analysis was performed by ERG and behavioral tests at 11, 24 and 54 weeks of age. OCT and histological analyses were performed to describe the retina morphology.Results: The ratio of transgenic pigs born after lentiviral-directed transgenesis was close to 50%. Transgenic pigs with 3 to 5 transgene copies per cell clearly present a reduced photopic response from 3 months of age on. Except for one pig, which has 6 integrated transgene copies, no dramatic decrease in general mobility was observed even at 6 months of age. OCT examinations reveal no major changes in the ONL structure of the 6-months old pigs. The retina morphology was well conserved in the 2 pigs sacrificed (3 and 6 months old) except a noticeable displacement of some cone nuclei in the outer segment layer.Conclusions: Lentiviral-directed transgenesis is a rapid and straightforward method to engineer transgenic pigs. Some Arr3-GUCY2DE837D/R838S pigs show signs of retinal dysfunction but further work is needed to describe the progression of the disease in this model.

Audit report on the Iowa Water Pollution Control Works Financing Program and the Iowa Drinking Water Facilities Financing Program, joint programs of the Iowa Finance Authority and the Iowa Department of Natural Resources for the year ended June 30, 2011

Audit report on the Iowa Water Pollution Control Works Financing Program and the Iowa Drinking Water Facilities Financing Program, joint programs of the Iowa Finance Authority and the Iowa Department of Natural Resources for the year ended June 30, 2011