Agro-Based Biocomposites for Industrial Applications

Leaf fibers are fibers that run lengthwise through the leaves of most monocotyledonous plants such as pineapple, banana, etc. Pineapple (Ananas comosus) and Banana (Musa indica) are emerging fiber having a very large potential to be used for composite materials. Over 150,000 ha of pineapple and over 100,000 ha of banana plantations are available in Brazil for the fruit production and enormous amount of agricultural waste is produced. This residual waste represents one of the single largest sources of cellulose fibers available at almost no cost. The potential consumers for this fiber are pulp and paper, chemical feedstock, textiles and composites for the automotive, furniture and civil construction industry.

Matrix effects observed during pesticides residue analysis in fruits by GC

The influence of the sample matrix in the CC-electron-capture detection analysis of the pesticides dimethoate, diazinon, chlorothalonil.. parathion methyl and fenitrothion in fruits samples has been studied. Experiments have been carried out where the pesticide responses in standard solutions prepared in selected solvent were compared with their response when present in apple, mango, papaya, banana, pineapple and melon extracts. The presence of matrix effects (MEs) and their extent were shown to be simultaneously influenced by several factors (matrix concentration, matrix type, pesticide concentration, analytical range). Pronounced MEs were observed particularly for dimethoate and diazinon in all matrices tested; in lower concentrations, all pesticides presented significant ME. The other pesticides presented variable ME. Higher ME enhancement was detected at lower pesticide concentration levels of and/or at higher matrix concentration solutions. The ME detected for fenitrothion, in the analytical range evaluated, were dependent on matrix type. For each pesticide, solvent and matrix-matched calibrations were compared for all fruit samples, and it could be concluded that quantitation based on standard solutions prepared in blank matrix extract (matrix-matched calibration) should be used to compensate the MEs and to obtain more accurate results for the pesticides studied.

Nuclear magnetic resonance water relaxation time changes in bananas during ripening: a new mechanism

BACKGROUND: Nuclear magnetic resonance studies of banana fragments during ripening show an increase on the water transverse relaxation time (T(2)) and a decrease in water self-diffusion coefficient (D). As T(2) and D are normally directly correlated, we studied these two properties in intact bananas during ripening, in an attempt to rule out the effect of injury on the apparent discrepancies in the behavior of T(2) and D. RESULTS: The results show that injury in bananas causes a decrease in T(2) of the water in vacuoles (T(2vac)). They also show that T(2vac) increased and D decreased during ripening, ruling out the injury effect. To explain the apparent discrepancies, we propose a new hypothesis for the increase in T(2) values, based on the reduction of Fe(3+) ions to Fe(2+) by galacturonic acid, produced by the hydrolysis of pectin and a decrease in internal oxygen concentration during ripening. CONCLUSION: As injury alters T(2) values it is necessary to use intact bananas to study relaxation times during ripening. The novel interpretation for the increase in T(2vac) based on reduction of Fe(+3) and O(2) concentration is an alternative mechanism to that based on the hydrolysis of starch in amyloplasts. (C) 2010 Society of Chemical Industry

An exploratory study on how to improve the economy of American Samoa

Deep in the South Pacific region about 2,300 miles southwest of the Hawaiian islands1 lies a United States territory that many Americans have never heard of nor known anything about. However, some famous Americans such as Troy Polamalu of the Pittsburgh Steelers, semi retired professional wrestler Dwayne “The Rock” Johnson, and Hawaii Congresswoman Tulsi Gabbard have genealogical roots there. More importantly, many of the Territory’s sons and daughters have served and lost their lives for the United States flag and the cause of freedom around the world. This place is called American Samoa, a collection of seven islands that if glued together would have a total landmass of approximately 76 square miles, just a tad bigger than the capital city of the United States. According to the United States Census Bureau, there were 55,519 residents of American Samoa in 2010.1 The majority of them are ethnic Samoans, a Polynesian sect that traces its history back to early migrants from Southeast Asia who settled the islands around 1500 B.C.2 3 The climate is warm all year long and the forests along the mountains are ripe with vegetation. The main island is Tutuila with its beautiful and coveted landlocked harbor that was used as a coaling station by the United States naval ships during World War II. In fact, it was the Pago Pago Harbor that diminished the impact of the 2009 Tsunami that devastated the Samoan islands by channeling the waters of the Pacific Ocean towards the end of the harbor instead of flooding many other villages surrounding the Pago Pago Bay area. Lives and property were destroyed near the end of the Harbor but it could have been worse for the entire Bay area. Locally grown foods include coconut, taro, banana, guava, sugar cane, papaya, yam, pineapple, and breadfruit. It is completely surrounded by the Pacific Ocean from which the locals obtain a variety of seafood. There is a popular saying in Samoa that goes, “In Samoa, it is impossible to starve 1 American Samoa Department of Commerce, 2012 Statistical Yearbook, http://www.doc.as/wpcontent/uploads/2011/06/2012-Statistical-Yearbook-1.pdf 2 U.S. Census Bureau News, U.S. Census Bureau Releases 2010 Census Population Counts for American Samoa, http://www.census.gov/2010census/news/releases/operations/cb11-cn177.html (Aug. 24, 2011). 3 3 J. Robert Shaffer, American Samoa: 100 Years Under the United States Flag (Honolulu, Hawaii: Island Heritage Publishing, 2000), 34. 4 because people live off of the land’s and the ocean’s abundant resources.” To the west of American Samoa lies a larger group of four islands that make up the Sovereign State of Samoa, which became independent from New Zealand in 1962. Samoa and American Samoa share the same language, culture, and religion but are divided by government and political systems. The focus of this study will be on American Samoa, which became a United States territory in 1900 when the principal chiefs of Tutuila (the largest island in American Samoa) ceded the islands to the United States.

Viabilidade econômica do cultivo de cacaueiro e bananeira irrigados no Maranhão

Este trabalho teve por objetivo estudar a viabilidade econômica da produção consorciada de cacau e banana na região do Perímetro Irrigado Tabuleiro de São Bernardo nos municípios de Magalhães de Almeida e Araioses, estado do Maranhão (MA), tendo em vista a ampla disponibilidade de recursos naturais para o cultivo e a potencialidade de ser uma região de escape das principais doenças que afetam as culturas de cacau e banana. O trabalho verifica a oportunidade de negócio de produção de cacau e banana na região do rio Parnaíba. O estudo utiliza projeções de fluxo de caixa e os desconta para o Valor Presente Líquido (VPL) para aferir a viabilidade econômica do negócio de produção de cacaueiro e bananeira nesta região do Brasil. Foram realizadas pesquisas bibliográficas para analisar as características edafoclimáticas da microrregião, as características fisiológicas do cacaueiro e seu consórcio com a bananeira para o sombreamento temporário necessário durante a fase de crescimento do cacaueiro. Foi sugerido o pacote de alta tecnologia para a produção de cacaueiro, dada a necessidade de irrigação para o seu cultivo. Diferentes produtividades e preços de cacau e banana foram utilizados para analisar a sensibilidade económica deste negócio. Ao projetar um fluxo de caixa, foi possível fazer uma análise sobre o negócio, viabilidade e riscos. Deve-se notar que não há grandes áreas produtoras de cacau na região do rio Parnaíba. Porém, há pequenas áreas de cacau em regiões semiáridas (ou de cerrado). Assim, a produção vegetal carece de comprovação de produção em larga escala para atestar de modo prático algumas das premissas econômico-financeiras adotadas para analisar a viabilidade da produção de cacau e banana no Perímetro Irrigado Tabuleiro de São Bernardo.

Estudo de caso das levadas agrícolas da Madeira

O ecoturismo é, hoje, uma tendência relevante no Turismo Mundial. Sendo a Região Autónoma da Madeira tão rica em recursos naturais, distinguida pela sua Laurissilva, como Património Mundial Natural da UNESCO, desde 1999, impõe-se a necessidade de explorar sustentadamente parte integrante da Ilha, de que a Levada Nova, situada no concelho da Calheta, é um exemplo significativo. Temos como objetivos principais promover o aproveitamento dos recursos físicos (casas de abrigo do IGA) e naturais já existentes, bem como dinamizar a Levada Nova na vertente turística, no pressuposto de que existem condições para a continuidade deste produto turístico no futuro, tendo em vista a sua sustentabilidade económica. Na metodologia empregue distinguem-se dois âmbitos, o teórico e o prático. Em termos teóricos, recorremos à revisão bibliográfica da temática em estudo. A componente prática baseou-se no trabalho de pesquisa, efetuado no campo, a fim de reconhecer, recolher dados para caraterização do espaço e elaborar o levantamento dos recursos suscetíveis de potenciação ecoturística. Num segundo momento, os dados foram organizados, através do programa de georeferenciação ArcGis, de modo a elaborar um roteiro turístico no espaço definido. Os dados obtidos através da observação direta no terreno - ao longo da Levada Nova - permitem-nos afirmar que a vegetação predominante engloba núcleos florestais de eucaliptos e pinheiros, existindo também pequenos bosquetes de Laurissilva, onde se destacam espécies como o til (Ocotea foetens), o loureiro (Laurus novocanariensis), o perado (Ilex perado), o folhado (Clethra arborea) e o vinhático (Persea indica). Os terrenos abaixo da Levada apresentam variedade nos produtos cultivados (banana, cana-de-açúcar, batata, feijão, vinha e pomares), em consequência de uma irrigação regular e do clima. Como conclusão verificamos a necessidade de uma maior dinamização turística no concelho da Calheta, que contribua para suster a decadência do comércio tradicional e o abandono do património.

Nematicidal activity of Solanum nigrum and S. sisymbriifolium extracts against the root-lesion nematode Pratylenchus goodeyi and its effects on infection and gene expression

The control of Pratylenchus goodeyi a common nematode parasite of banana crop in Madeira Island can benefit from searching for natural nematicides through plants extracts. With this aim we submitted Solanum nigrum and S. sisymbriifolium dried plants to a sequential extraction in the solvent sequence of dichloromethane, acetone, ethanol and water, and to na aqueous extraction of the fresh and dried plants. Analyses with the extracts at several concentrations were used to assess mobility and mortality on P. goodeyi. Results showed that the water extract and aqueous extracts from both plants at a concentration of 10 mg/mL affected nematode mobility and caused mortality but the acetone extract from S. nigrum was the most efficient, causing 100% mortality whereas dichloromethane had no effect on P. goodeyi. Determination of the lipophilic and phenolic compounds present in the two most effective Solanum extracts (acetone and water) and in dichloromethane extract revealed that some of these compounds had nematicidal activity. S. nigrum acetone extract (10 mg/mL) was used to find out the nematicidal potential following the effect at gene expression level and nematode behaviour. Genes coding for calreticulin and beta-1,4- endoglucanase related to parasitism and translocon-associated protein putatively connected to stress were obtained and its relative expression assessed in nematodes exposed to the extract. Results revealed that expression of Pg-CRT decreased showing to influence the infection, Pg-ENG remained steady and Pg-TRAPδ was induced over time exposure. Biological assays showed that P. goodeyi mobility and ability to infect the banana roots were affected as a decrease in the number of nematodes that reached the roots was obtained with the increased exposure time to the extract being implicated in the infection success. The information obtained from this thesis showed that S. nigrum has potential to be used for the development of a new control strategy against plant-parasitic nematodes.

Comparative study of the whisky aroma profile based on headspace solid phase microextraction using different fibre coatings

A dynamic headspace solid-phase microextraction (HS-SPME) and gas chromatography coupled to ion trap mass spectrometry (GC–ITMS) method was developed and applied for the qualitative determination of the volatile compounds present in commercial whisky samples which alcoholic content was previously adjusted to 13% (v/v). Headspace SPME experimental conditions, such as fibre coating, extraction temperature and extraction time, were optimized in order to improve the extraction process. Five different SPME fibres were used in this study, namely, poly(dimethylsiloxane)(PDMS),poly(acrylate)(PA),Carboxen-poly(dimethylsiloxane)(CAR/PDMS),Carbowax-divinylbenzene(CW/DVB)and Carboxen-poly(dimethylsiloxane)-divinylbenzene (CAR/PDMS/DVB). The best results were obtained using a 75 m CAR/PDMS fibre during headspace extraction at 40◦C with stirring at 750rpm for 60min, after saturating the samples with salt. The optimised methodology was then appliedtoinvestigatethevolatilecompositionprofileofthreeScotchwhiskysamples—BlackLabel,BallantinesandHighlandClan.Approximately seventy volatile compounds were identified in the these samples, pertaining at several chemical groups, mainly fatty acids ethyl esters, higher alcohols, fatty acids, carbonyl compounds, monoterpenols, C13 norisoprenoids and some volatile phenols. The ethyl esters form an essential group of aroma components in whisky, to which they confer a pleasant aroma, with “fruity” odours. Qualitatively, the isoamyl acetate, with “banana” aroma,wasthemostinteresting.Quantitatively,significantcomponentsareethylestersofcaprilic,capricandlauricacids.Thehighestconcentration of fatty acids, were observed for caprilic and capric acids. From the higher alcohols the fusel oils (3-methylbutan-1-ol and 2.phenyletanol) are the most important ones.

Headspace solid-phase microextraction-gas chromatography-quadrupole mass spectrometric methodology for the establishment of the volatile composition of Passiflora fruit species

Dynamic headspace solid-phase microextraction (HS-SPME) followed by thermal desorption gas chromatography-quadrupole mass spectrometry analysis (GC-qMS), was used to investigate the aroma profile of different species of passion fruit samples. The performance of five commercially available SPME fibres: 65 μm polydimethylsiloxane/divinylbenzene, PDMS/DVB; 100 μm polydimethylsiloxane, PDMS; 85 μm polyacrylate, PA; 50/30 μm divinylbenzene/carboxen on polydimethylsiloxane, DVB/CAR/PDMS (StableFlex); and 75 μm carboxen/polydimethylsiloxane, CAR/PDMS; was evaluated and compared. Several extraction times and temperature conditions were also tested to achieve optimum recovery. The SPME fibre coated with 65 μm PDMS/DVB afforded the highest extraction efficiency, when the samples were extracted at 50 °C for 40 min with a constant stirring velocity of 750 rpm, after saturating the sample with NaCl (17%, w/v — 0.2 g). A comparison among different passion fruit species has been established in terms of qualitative and semi-quantitative differences in volatile composition. By using the optimal extraction conditions and GC-qMS it was possible to tentatively identify seventy one different compounds in Passiflora species: 51 volatiles in Passiflora edulis Sims (purple passion fruit), 24 in P. edulis Sims f. flavicarpa (yellow passion fruit) and 21 compounds in Passiflora mollissima (banana passion fruit). It was found that the ethyl esters comprise the largest class of the passion fruit volatiles, including 82.8% in P. edulis variety, 77.4% in P. edulis Sims f. flavicarpa variety and 39.9% in P. mollissima. The semi-quantitative results were then submitted to principal component analysis (PCA) in order to establish relationships between the compounds and the different passion fruit species under investigation.

Flebotomíneos (Diptera: Psychodidae) em um sistema agroflorestal da Região Metropolitana de Natal, Rio Grande do Norte, Brasil

Sandflies stand out as important vectors of leishmaniasis. The females need to ingest blood meals, enabling them to transmit protozoa of the genus Leishmania, which may give rise to visceral leishmaniasis (VL) or American tegumentary leishmaniasis (ATL), in addition to transmitting other parasites. Leishmaniasis are important infirmities, distributed worldwide, whose infection results from the interaction of reservoir animals, the vector insect, parasitic protozoa and the healthy host. In the state of Rio Grande do Norte (RN), Brazil, these insects are important transmitters of VL, which usually presents in the most serious form. It occurs mainly in metropolitan areas, with the dog as its main reservoir and Lutzomyia longipalpis as the vector. ATL is most present in the highland areas of the state. In addition to hematophagia, engaged in by the females, both sexes need to ingest carbohydrates, which are essential to the sand flies energy requirements and may interfere in the development of Leishmania. The aim of this study was to determine the occurrence and abundance of sand flies in different environments on the farm belonging to the Empresa de Pesquisas Agropecuárias do RN (Institute of Agricultural Research of RN), in the municipality of Parnamirim, in order to relate this occurrence with climatological and biological references and eating habits. Three consecutive monthly collections were carried out with CDC traps in a fragment of the Atlantic Forest, in a residence, on a goat breeding farm and on cashew, dwarf and giant coconut, mango, banana, eucalyptus, acacia and bean plantations. A total of 1241 sandflies from eight species (Lutzomyia evandroi, Lutzomyia longipalpis, Lutzomyia shannoni, Lutzomyia sordellii Lutzomyia walkeri, Lutzomyia wellcomei, Lutzomyia whitmani, and Lutzomyia intermedia) were collected, most in the forest environment. L. longipalpis, the main VL transmitter, was confirmed as a species adapted to anthropic environments, whereas others such as L. wellcomei, the vector of ATL, occurred predominantly in forests. Carbohydrate characterization of the sand flies and plants of the region demonstrated that a number of exotic plants such as hay and eucalyptus may play some role in the adaptation of these species to modified environments. Breeding in laboratory showed a mean biological cycle of 53.5 days from egg to adulthood for L. shannoni and the possibility of diapause behavior in L. wellcomei. This study serves as a source of information that may contribute to the epidemiological vigilance of tegumentary and visceral leishmaniasis in the state, given that it analyzes the bioecology of transmitting species, as well as their potential to adapt to new environments

Cadastramento fitossociológico de plantas daninhas na bananicultura

O objetivo deste experimento, realizado na cultura da banana (Musa spp.) no Vale do Ribeira, no município de Registro-SP, foi fazer um cadastramento fitossociológico das espécies de plantas daninhas sob duas formas de manejo do solo. A amostragem das espécies daninhas foi feita em duas áreas distintas de manejo, sendo uma com a cultura implantada em área de várzea drenada a 7 m de altitude e a outra com a bananicultura em área de sequeiro a 16 m de altitude. Na amostragem de um hectare, utilizou-se o método do quadrado inventário para cálculo de frequência, frequência relativa, densidade, densidade relativa, abundância, abundância relativa, índice de valor de importância e índice de importância relativa. em ambas as áreas foram identificadas 10 famílias, distribuídas em 18 gêneros e 21 espécies. Na área de várzea drenada, 38% das famílias identificadas são monocotiledôneas e 62% dicotiledôneas, num total de 15 espécies, distribuídas em nove famílias. Na área de cultivo em sistema de sequeiro, foram identificados 50% de famílias monocotiledôneas e 50% de dicotiledôneas, num total de 11 espécies, distribuídas em seis famílias. As famílias com maior representatividade foram Poaceae, com sete espécies, seguida de Asteraceae, com três. Com o estudo realizado, verificou-se ainda grande diversidade de espécies nas áreas selecionadas.

Resistência do solo à penetração e ao cisalhamento em diversos usos do solo em áreas de preservação permanente

The soil resistance to penetration and shear can be used as indicators of soil compaction and to indicate the susceptibility of a soil to erosion. The objective of this study was to quantify and compare the impact provided by different land uses in a haplic cambissol in areas of permanent preservation, from the soil resistance to penetration and shear. The experimental area was located in an area of permanent preservation, the sub-basin of the Ribeira Iguape River - SP, with different land uses: banana cultivation - CBAN, degraded pasture - PDEG, silvopastoral system - MPIS and native forest - MNAT. The test for resistance to penetration was accomplished with a digital penetrometer compaction of manual effort, to a depth of 40 cm. The soil shear strength was determined by Vane Test at a depth between 0 and 5 cm. The degraded pasture was similar to native forest, with less resistance to penetration. The banana cultivation and silvopastoral system were the land uses with the highest resistance to penetration, bringing serious risk of erosion in areas of permanent preservation. The soil under native forest had lower shear strength. The cultivation of bananas, degraded pasture and silvopastoral system were the land uses with higher shear strength of soil, indicating that the use of these soils in areas of permanent preservation is promoting the same compression.

Respostas fisiológicas em mudas de banananeira tratadas com estrobilurinas

There are reports that strobilurin besides having a fungicide effect can promote physiologic benefits to the plants. However, this effect on banana plants was not studied yet. The objective of the present study was to evaluate the effect of strobirulins on the physiology of banana plantlets. For this purpose, cultivar Grand Naine banana plantlets were transferred to pots containing substrate and kept in a nursery with 50% shading. The experimental design was a completely randomized design with three treatments (water, azoxystrobin and pyraclostrobin) and five replications. The treatments were applied at 15, 30, 45, 60 and 75 days after transplanting at a dose 100 g a. i. ha(-1) with manual spray. Plant height, pseudostem diameter, shoot dry matter in strobilurin treated plants were higher than the untreated plants, however, the effect of fungicide treatment was different, being the most pronounced effect of pyraclostrobin compared to azoxystrobin. Plants treated with pyraclostrobin had higher leaf area, nitrate reductase activity and chlorophyll content of leaf total nitrogen than the plants treated with azoxystrobin and water, which did not differ. Strobilurins affect the physiology of the banana plantlets differently, the effect being more pronounced by pyraclostrobin.

Variação sazonal da concentração de nutrientes em folhas de bananeiras, no Vale do Ribeira-SP

The Vale do Ribeira, SP, main agricultural activity is the banana crop, which accounts for most of this fruit production in the State of São Paulo. The nutritional balance of the plant is one of the most important factors for the banana plant can complete the cycle and achieve high productivity. Aiming to evaluate the seasonal variation of leaf nutrient concentration in banana plants in Vale do Ribeira-SP, we used the results of 252 chemical analyses of plant tissue, collected from August 2009 to September 2010, in the 18 representative properties for the region, ten cultivated with subgroup Cavendish banana plant and eight of subgroup Prata banana plant. The largest variation between the macronutrient occurred for K and S, and among the micronutrients, especially for Fe and B. In some dates of evaluation, there was a higher leaf concentration of P, K, Ca and Zn, in subgroup Cavendish banana plants, while the subgroup Prata banana plants showed higher leaf concentration, especially of Mn, B and N. Climatic conditions, especially rain, influenced the leaf nutrient content, especially for K, N, S, B and Fe.

Influence of stocking densities in the nursery phase on the growth of Macrobrachium amazonicum reared in net pens

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)