Agreed-upon procedures report on the City of Blue Grass, Iowa for the period July 1, 2013 through June 30, 2014

Agreed-upon procedures report on the City of Blue Grass, Iowa for the period July 1, 2013 through June 30, 2014

Agreed-upon procedures report on the City of Bedford, Iowa for the period July 1, 2013 through June 30, 2014

Agreed-upon procedures report on the City of Bedford, Iowa for the period July 1, 2013 through June 30, 2014

Agreed-upon procedures report on the City of Pleasant Plain, Iowa for the period January 1, 2014 through December 31, 2014

Agreed-upon procedures report on the City of Pleasant Plain, Iowa for the period January 1, 2014 through December 31, 2014

Agreed-upon procedures report on the City of Patterson, Iowa for the period December 1, 2013 through November 30, 2014

Agreed-upon procedures report on the City of Patterson, Iowa for the period December 1, 2013 through November 30, 2014

Agreed upon procedures report on the City of Galt, Iowa for the period August 1, 2013 through July 31, 2014

Agreed upon procedures report on the City of Galt, Iowa for the period August 1, 2013 through July 31, 2014

Agreed-upon procedures report on the City of Cambridge, Iowa for the period August 1, 2013 through July 31, 2014

Agreed-upon procedures report on the City of Cambridge, Iowa for the period August 1, 2013 through July 31, 2014

Agreed-upon procedures report on the City of Gilman, Iowa for the period September 1, 2013 through August 31, 2014

Agreed-upon procedures report on the City of Gilman, Iowa for the period September 1, 2013 through August 31, 2014

Agreed-upon procedures report on the City of Mount Ayr, Iowa for the period July 1, 2013 through June 30, 2014

Agreed-upon procedures report on the City of Mount Ayr, Iowa for the period July 1, 2013 through June 30, 2014

High-throughput and sensitive quantitation of plasma catecholamines by ultraperformance liquid chromatography-tandem mass spectrometry using a solid phase microwell extraction plate.

Plasma catecholamines provide a reliable biomarker of sympathetic activity. The low circulating concentrations of catecholamines and analytical interferences require tedious sample preparation and long chromatographic runs to ensure their accurate quantification by HPLC with electrochemical detection. Published or commercially available methods relying on solid phase extraction technology lack sensitivity or require derivatization of catecholamine by hazardous reagents prior to tandem mass spectrometry (MS) analysis. Here, we manufactured a novel 96-well microplate device specifically designed to extract plasma catecholamines prior to their quantification by a new and highly sensitive ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method. Processing time, which included sample purification on activated aluminum oxide and elution, is less than 1 h per 96-well microplate. The UPLC-MS/MS analysis run time is 2.0 min per sample. This UPLC-MS/MS method does not require a derivatization step, reduces the turnaround time by 10-fold compared to conventional methods used for routine application, and allows catecholamine quantification in reduced plasma sample volumes (50-250 μL, e.g., from children and mice).

Agreed-upon procedures report on the City of Lacona, Iowa for the period December 1, 2013 through November 30, 2014

Agreed-upon procedures report on the City of Lacona, Iowa for the period December 1, 2013 through November 30, 2014

Agreed-upon procedures report on the City of Lansing, Iowa for the period July 1, 2013 through June 30, 2014

Agreed-upon procedures report on the City of Lansing, Iowa for the period July 1, 2013 through June 30, 2014

Agreed-upon procedures report on the City of Armstrong, Iowa for the period October 1, 2013 through September 30, 2014

Agreed-upon procedures report on the City of Armstrong, Iowa for the period October 1, 2013 through September 30, 2014

Stabilization Procedures to Mitigate Edge Rutting for Granular Shoulders - Phase II, TR-591, 2011

A multifaceted investigation was undertaken to develop recommendations for methods to stabilize granular road shoulders with the goal of mitigating edge ruts. Included was reconnaissance of problematic shoulder locations, a laboratory study to develop a method to test for changes in granular material stability when stabilizing agents are used, and the construction of three sets of test sections under traffic at locations with problematic granular shoulders. Full results of this investigation are included in this report and its appendices. This report also presents conclusions and recommendations based on the study results.

Agreed-upon procedures report on the City of Hills, Iowa for the period October 1, 2013 through September 30, 2014

Agreed-upon procedures report on the City of Hills, Iowa for the period October 1, 2013 through September 30, 2014

Agreed-upon procedures report on the City of Kelley, Iowa for the period November 1, 2013 through October 31, 2014

Agreed-upon procedures report on the City of Kelley, Iowa for the period November 1, 2013 through October 31, 2014