Effects of nutrition on digestión efficiency and gaseous emissions from slurry in growing pigs: III. Influence of varying the dietary level of calcium soap of palm fatty acids distillate with or without orange pulp supplemention

The aim of this study was to establish the relationships between faecal fat concentration and gaseous emissions from pig slurry. Five diets were designed to meet essential nutrient requirements: a control and four experimental feeds including two levels (35 or 70 g/kg) of calcium soap fatty acids distillate (CSP) and 0 or 200 g/kg of orange pulp (OP) combined in a 2 × 2 factorial structure. Thirty growing pigs (six per treatment) were used to measure dry matter (DM) and N balance, coefficients of total tract apparent digestibility (CTTAD) of nutrients, faecal and urine composition and potential emissions of ammonia (NH3) and methane (CH4). Increasing dietary CSP level decreased DM, ether extract (EE) and crude protein (CP) CTTAD (by 4.0, 11.1 and 3.5%, respectively, P < 0.05), but did not influence those of fibrous constituents. It also led to a decrease (from 475 to 412 g/kg DM, P < 0.001) of faecal concentration of neutral detergent fibre (aNDFom) and to an increment (from 138 to 204 g/kg, P < 0.001) of EE in faecal DM that was related to greater CH4 emissions, both per gram of organic matter (P = 0.021) or on a daily basis (P < 0.001). Level of CSP did not affect N content in faeces or urine, but increased daily DM (P < 0.001), and N (P = 0.031) faecal excretion with no effect on urine N excretion. This resulted in lesser (P = 0.036) NH3 potential emission per kg of slurry. Addition of OP decreased CTTAD of EE (by 7.9%, P = 0.044), but increased (P < 0.05) that of all the fibrous fractions. As a consequence, faecal EE content increased (from 165 to 177 g/kg DM; P = 0.012), and aNDFom decreased greatly (from 483 to 404 g/kg DM, P < 0.001), which in all resulted in a lack of effect of OP on CH4 potential emission. Inclusion of OP in the diet also led to a significant decrease of CP CTTAD (by 6.85%, P < 0.001), and to an increase of faecal CP concentration (from 174 to 226 g/kg DM, P < 0.001), with no significant influence on urine N content. These effects resulted in higher N faecal losses, especially those of the undigested dietary origin, without significant effects on potential NH3 emission. No significant interactions between CSP and OP supplementation were observed for the gaseous emissions measured.

Protonatable residues at the cytoplasmic end of transmembrane helix-2 in the signal transducer HtrI control photochemistry and function of sensory rhodopsin I.

Neutral residue replacements were made of 21 acidic and basic residues within the N-terminal half of the Halobacterium salinarium signal transducer HtrI [the halobacterial transducer for sensory rhodopsin I (SRI)] by site-specific mutagenesis. The replacements are all within the region of HtrI that we previously concluded from deletion analysis to contain sites of interaction with the phototaxis receptor SRI. Immunoblotting shows plasmid expression of the htrI-sopI operon containing the mutations produces SRI and mutant HtrI in cells at near wild-type levels. Six of the HtrI mutations perturb photochemical kinetics of SRI and one reverses the phototaxis response. Substitution with neutral amino acids of Asp-86, Glu-87, and Glu-108 accelerate, and of Arg-70, Arg-84, and Arg-99 retard, the SRI photocycle. Opposite effects on photocycle rate cancel in double mutants containing one replaced acidic and one replaced basic residue. Laser flash spectroscopy shows the kinetic perturbations are due to alteration of the rate of reprotonation of the retinylidene Schiff base. All of these mutations permit normal attractant and repellent signaling. On the other hand, the substitution of Glu-56 with the isosteric glutamine converts the normally attractant effect of orange light to a repellent signal in vivo at neutral pH (inverted signaling). Low pH corrects the inversion due to Glu-56 -> Gln and the apparent pK of the inversion is increased when arginine is substituted at position 56. The results indicate that the cytoplasmic end of transmembrane helix-2 and the initial part of the cytoplasmic domain contain interaction sites with SRI. To explain these and previous results, we propose a model in which (i) the HtrI region identified here forms part of an electrostatic bonding network that extends through the SRI protein and includes its photoactive site; (ii) alteration of this network by photoisomerization-induced Schiff base deprotonation and reprotonation shifts HtrI between attractant and repellent conformations; and (iii) HtrI mutations and extracellular pH alter the equilibrium ratios of these conformations.

Residue replacements of buried aspartyl and related residues in sensory rhodopsin I: D201N produces inverted phototaxis signals.

Residue replacements were made at five positions (Arg-73, Asp-76, Tyr-87, Asp-106, and Asp-201) in the Halobacterium salinarium phototaxis receptor sensory rhodopsin I (SR-I) by site-specific mutagenesis. The sites were chosen for their correspondence in position to residues of functional importance in the homologous light-driven proton pump bacteriorhodopsin found in the same organism. This work identifies a residue in SR-I shown to be of vital importance to its attractant signaling function: Asp-201. The effect of the substitution with the isosteric asparagine is to convert the normally attractant signal of orange light stimulation to a repellent signal. In contrast, similar neutral substitution of the four other ionizable residues near the photoactive site allows essentially normal attractant and repellent phototaxis signaling. Wild-type two-photon repellent signaling by the receptor is intact in the Asp-201 mutant, genetically separating the wild-type attractant and repellent signal generation processes. A possible explanation and implications of the inverted signaling are discussed. Results of neutral residue substitution for Asp-76 confirm our previous evidence that proton transfer reactions involving this residue are not important to phototaxis but that Asp-76 functions as the Schiff base proton acceptor in proton translocation by transducer-free SR-I.

William J. Olcott, University of Michigan football captain 1882, 1883

Cropped from 1883 team photo.

William C. Malley, 1890 University of Michigan football captain

Cropped from 1889 team photo.

Memoir of the operations of the allied armies, under Prince Schwarzenberg, and Marshal Blucher, during the latter end of 1813, and the year 1814,

Mode of access: Internet.

I. An exposition of the symbolic terms of the second part of Faust. II. How this part thus proves itself to be a dramatic treatment of the modern history of Germany worthy of the genius of Goethe and the life he devoted to the task. (The prologues and first act completely explained; the other acts generally).

Mode of access: Internet.

Briefwechsel zwischen König Johann von Sachsen und den Königen Friedrich Wilhelm IV. und Wilhelm I. von Preussen,

Mode of access: Internet.

The works of Henry Mackenzie, esq. ...

I. Man of feeling. Man of the world.--II. Man of the world (cont.)--III. Julia de Roubigné.--IV. Papers from The Mirror.--V. Papers from The Mirror (cont.) Papers from The Lounger.--VI. Papers from The Lounger (cont.)--VII. Papers from The Lounger (cont.) Life of Dr. Blacklock. Life of Lord Abercromby. Life of William Tytler. Review of the principal proceedings of the Parliament of 1784.--VIII. Poems. Dramatic pieces: The prince of Tunis. The Spanish father. False shame, or The white hypocrit.

Dramatic works of William Shakspeare, with a life of the poet and notes, original and selected.

v.2. Midsummer-night's dream. Love's labor's lost. Merchant of Venice. As y@u like it. All's well that ends well. Taming of the shrew.--v.3. Winter's tale. Comedy of errors. Macbeth. King John. King Richard the second. King Henry the fourth, pt. 1st.--v.4. King Henry the fourth, pt. 2d. King Henry the fifth. King Henry the sixth, pt. 1st - 3d.--v.5. King Richard the third. King Henry the eighth. Troilus and Cressida. Timon of Athens. Coriolanus.--v.7. King Lear. Romeo and Juliet. Hamlet, prince of Denmark. Othello, the Moor of Venice.

The complete works of William Shakespeare,

At head of title: New national edition.

A survey of the Turkish empire. In which are considered I. Its government ... II. The state of the provinces ... III. The causes of the decline of Turkey ... IV. The British commerce with Turkey ... With many other important particulars.

Mode of access: Internet.

The History of the life and reign of the valiant Prince Edward, afterwards King Edward the first of England, son to King Henry the third; and his Princess Eleonora. On which history is founded a play written by Mr. Thomson, call'd Edward and Eleonora; now in rehearsal at the Theatre in Covent-Garden. Extracted from the best historians. With a geographical description of that Prince's expedition to the Holy Land.

Mode of access: Internet.

The chorus and choir : a superior collection of standard choruses, part-songs and anthems suitable for colleges, normals, choirs, choral societies, musical conventions, singing schools, etc., etc. /

Includes index.