Transposon tools for recombinant DNA manipulation: characterization of transcriptional regulators from yeast, Xenopus, and mouse.

Transposon Tn1000 has been adapted to deliver novel DNA sequences for manipulating recombinant DNA. The transposition procedure for these "tagged" Tn1000s is simple and applicable to most plasmids in current use. For yeast molecular biology, tagged Tn1000s introduce a variety of yeast selective markers and replication origins into plasmids and cosmids. In addition, the beta-globin minimal promoter and lacZ gene of Tn(beta)lac serve as a mobile reporter of eukaryotic enhancer activity. In this paper, Tn(beta)lac was used to localize a mouse HoxB-complex enhancer in transgenic mice. Other tagged transposons create Gal4 DNA-binding-domain fusions, in either Escherichia coli or yeast plasmids, for use in one- and two-hybrid tests of transcriptional activation and protein-protein interaction, respectively. With such fusions, the Saccharomyces cerevisiae Swi6 G1/S-phase transcription factor and the Xenopus laevis Pintallavis developmental regulator are shown to activate transcription. Furthermore, the same transposon insertions also facilitated mapping of the Swi6 and Pintallavis domains responsible for transcriptional activation. Thus, as well as introducing novel sequences, tagged transposons share the numerous other applications of transposition such as producing insertional mutations, creating deletion series, or serving as mobile primer sites for DNA sequencing.

An auxiliary factor containing a 240-kDa protein complex is involved in apolipoprotein B RNA editing.

A protein complex involved in apolipoprotein B (apoB) RNA editing, referred to as AUX240 (auxiliary factor containing p240), has been identified through the production of monoclonal antibodies against in vitro assembled 27S editosomes. The 240-kDa protein antigen of AUX240 colocalized with editosome complexes on immunoblots of native gels. Immunoadsorbed extracts were impaired in their ability to assemble editosomes beyond early intermediates and in their ability to edit apoB RNA efficiently. Supplementation of adsorbed extract with AUX240 restored both editosome assembly and editing activities. Several proteins, in addition to p240, ranging in molecular mass from 150 to 45 kDa coimmunopurify as AUX240 under stringent wash conditions. The activity of the catalytic subunit of the editosome APOBEC-1 and mooring sequence RNA binding proteins of 66 and 44 kDa could not be demonstrated in AUX240. The data suggest that p240 and associated proteins constitute an auxiliary factor required for efficient apoB RNA editing. We propose that the role of AUX240 may be regulatory and involve mediation or stabilization of interactions between APOBEC-1 subunits and editing site recognition proteins leading the assembly of the rat liver C/U editosome.

Social License to Operate: Hydraulic Fracturing-Related Challenges Facing the Oil & Gas Industry

The crossroads of urban development and improved technology allowing oil and gas development in new areas can result in contentious community issues. The debate over one of the improved technologies – i.e., hydraulic fracturing – can be highly emotional. Consequently, industry must address community issues, earning trust and therefore a “social license to operate.” This paper provides fundamental knowledge of the social license to operate concept, validates its application to the oil and gas industry, particularly with respect to shale gas development, discusses the current status of social license in the unconventional development sphere, analyzes current ongoing efforts for shale gas developers to monitor and establish a social license, and identifies potential new methods of encouraging, establishing, and monitoring a social license to operate. The paper also proposes a new institutional framework in which to promote the social license to operate, “The Center for Social License to Operate in the Oil & Gas Industry.”

The University prints. Oriental art. Series O ...

Section I. Early Indian and Indonesian art, ed. by Dr. Benjamin Rowland, jr.--Section II. Early Chinese art, ed. by L. C. S. Sickman.--Section III. Japanese art, ed. by H. G. Henderson and R. T. Paine, jr.--Section IV. Iranian and Islamic art, ed. by Richard Ettinghausen and Eric Schroeder.

Airborne radiometric survey in the Lemitar-Ladron area, New Mexico /

Three areas of Tertiary sedimentary and igneous rocks in northwestern Socorro County, New Mexico, were covered by an airborne radiometric survey in April 1955. The survey failed to reveal any new areas of anomalous radioactivity.

Circular /

Description based on: No. 3 (Aug. 28, 1895); title from caption.

Raban : or, Life-splinters /

Mode of access: Internet.

The animal parasite supposed to be the cause of yellow fever /

Cover title.

The truth defended, or, A reply to Elder D.H. Bays' Doctrines and dogmas of Mormonism.

Mode of access: Internet.

Rambles round Guildford, with a topographical and historical description of the town.

Mode of access: Internet.

As you like it /

Mode of access: Internet.

As you like it /

Mode of access: Internet.

Religious pamphlets

A collection of miscellaneous pamphlets on religion.

Religious pamphlets

A collection of miscellaneous pamphlets on religion.

Gabriel Harvey's Marginalia,

Introduction: [A short study of Harvey's life and character]: p.4-76.