Clinicopathological Phenotype of Autosomal Recessive Cholesterol Deficiency in Holstein Cattle.

BACKGROUND Cholesterol deficiency (CD), a newly identified autosomal recessive genetic defect in Holstein cattle, is associated with clinical signs of diarrhea, failure to thrive, and hypocholesterolemia. HYPOTHESIS/OBJECTIVES The objective is to describe the clinicopathological phenotype of affected Holstein cattle homozygous for the causative apolipoprotein B gene (APOB) mutation. ANIMALS Six Holstein cattle, 5 calves with a clinical history of chronic diarrhea, and 1 heifer with erosions in the buccal cavity and neurologic symptoms were admitted to the Clinic for Ruminants. METHODS This case review included a full clinical examination, a complete blood count, blood chemistry, and measurements of cholesterol and triglycerides. The animals were euthanized and necropsied. A PCR-based direct gene test was applied to determine the APOB genotype. RESULTS All 6 animals were inbred, could be traced back to the sire Maughlin Storm, and were confirmed homozygous for the APOB mutation. The clinical phenotype included poor development, underweight, and intermittent diarrhea in the calves, and neurologic signs in the heifer included hypermetria and pacing. Hypocholesterolemia and low triglycerides concentrations were present in all animals. The pathological phenotype of all animals was steatorrhea with enterocytes of the small intestine containing intracytoplasmic lipid vacuoles. The peripheral nervous system of the heifer displayed degenerative changes. CONCLUSIONS AND CLINICAL IMPORTANCE Suspicion of CD in Holstein cattle is based on the presence of chronic diarrhea with no evidence of primary infections. Confirmation of the associated APOB gene mutation is needed. Additionally, the heifer demonstrated primarily signs of neurologic disease providing an unexpected phenotype of CD.

Proglacial river dataset from the Akuliarusiarsuup Kuua River northern tributary, Southwest Greenland, 2008 - 2010, version 1.0

Pressing scientific questions concerning the Greenland ice sheet's climatic sensitivity, hydrology, and contributions to current and future sea level rise require hydrological datasets to resolve. While direct observations of ice sheet meltwater losses can be obtained in terrestrial rivers draining the ice sheet and from lake levels, few such datasets exist. We present a new dataset of meltwater river discharge for the vicinity of Kangerlussuaq, Southwest Greenland. The dataset contains measurements of river stage and discharge for three sites along the Akuliarusiarsuup Kuua (Watson) River's northern tributary, with 30 minute temporal resolution between June 2008 and August 2010. Additional data of water temperature, air pressure, and lake water depth and temperature are also provided. Discharge data were measured at sites with near-ideal properties for such data collection. Regardless, high water bedload and turbulent flow introduce considerable uncertainty. These were constrained and quantified using statistical techniques, thereby providing a high quality dataset from this important site. The greatest data uncertainties are associated with streambed elevation change and measurements. Large portions of stream channels deepened according to statistical tests, but poor precision of streambed depth measurements also added uncertainty. Quality checked data are freely available for scientific use as supplementary online material.

How to write criterion-referenced tests for Spanish-English bilingual programs /

"The project reported herein was performed pursuant to a grant from the U.S. Office of Education, Department of Health, Education and Welfare."

Guide to Porteus maze test.

Mode of access: Internet.

Mental tests of unemployed men. /

Reprinted from the Journal of applied psychology, December, 1917, vol. I., March, 1918, vol. II.

Standard tests as aids in school supervision, illustrated by a study of the Stoughton, Wisconsin, schools,

Mode of access: Internet.

Introduction to measurement,

Mode of access: Internet.

The growing edge,

Mode of access: Internet.

Detroit tests of learning aptitude; a new instrument of mental diagnosis: examiner's handbook,

These tests were developed in the Detroit psychological clinic, a department of the Detroit public schools. cf. Pref.

Mathematics assessment report, Spring 1977 /

WI docs. no.: Ed.3/2:9320

The value of supervision in the rural schools of Oakland County ...

At head of title: Michigan Education Association.

E.R.B. Handbook for independent schools

Autographed from typewritten copy.

Report, Spring 1977 Wisconsin Pupil Assessment Program.

WI docs. no.: Ed.3/2:9298

Beni Hassan.

Published by the Egyptian Exploration Fund.

Results of the testing program in the Grosse Ile public school,

"References": p. 71.