CETA and rural areas : an assessment of the implementation of balance of state prime sponsors of the Comprehensive employment and training act of 1973 in rural America.

Includes bibliographical references.

Employment and training programs for offenders : a guide for prime sponsors under the Comprehensive employment and training act of 1973 /

Title on inside leaf: Technical assistance guide for offender programs.

I'industria tartarica; materie prime derivanti dal vino.

Mode of access: Internet.

Selling to Navy prime contractors

Mode of access: Internet.

Introduction to general chemistry. A graded course of one hundred lectures with an atlas of eighty plates, representing chemists, institutions, prime materials, crystals, diagrams and apparatus; and illustrations in the text

Mode of access: Internet.

Communicable disease and immunization programs. Hearing, Ninety-first Congress, second session ... March 13, 1970.

Hearings on H.R. 11913, 12067, 12116, 12153, 13482, 15961, 16168, and S. 2264.

Safety code for mechanical power-transmission apparatus : rules for guarding prime movers, intermediate equipment, and driven machines : approved by American Standards Association as an American standard, 1927 /

"ASA B15-1927"--Cover.

The windmill as a prime mover.

1st thousand.

Polisen i förändring : En fallstudie om Polismyndighetens upphandling av kommunikationstjänster från PR-byrån Prime

Polismyndigheten har under 2015 påbörjat och ska under 2016 fortsätta undergå en omorganisering. En del av omorganiseringen innefattar att Polismyndigheten skrivit ett fyraårigt kontrakt värderat till 35 miljoner kronor med Prime Public Relations AB med avseende upphandling av strategiska kommunikationstjänster. Studien syftar till att undersöka vad skälen är till att Polismyndigheten har valt att en PR-byrå ska sköta delar av deras kommunikation och vad det hittills fått för konsekvenser. Totalt analyseras 50 artiklar som publicerats under upphandlingstiden i en innehållsanalys för att få en överblick kring olika teman som framkommit. Innehållsanalysen fungerar som ett kvantitativt komplement till två semi-strukturerade intervjuer samt en mailkorrespondens med projektansvarig för samarbetet med Polismyndigheten på Prime. En av intervjuerna hölls med en tidigare presstalesperson för Polismyndigheten som nu är kommunikationskonsult med uppdrag inom bland annat kriskommunikation. Den andra intervjun hölls med ansvarig handläggare för upphandlingen hos Polismyndigheten. En enkät kompletterar materialet med utomstående åsikter kring förändring av förtroendet för polisen i samband med upphandlingen. Tillsammans bidrar metoderna till ett gediget material för fallstudien som blir belyst från olika håll både med hjälp av kvalitativ samt kvantitativ data. Resultaten leder fram till en diskussion kring organisationskommunikation och intern kritik från anställda poliser som en produkt av upphandlingen. Analysen tar även upp de anställdas meningsskapande av den nya kontexten inom myndigheten samt en diskussion om förtroende och huruvida situationen kring upphandlingen skulle kunna ha sköts på ett annat sätt för att undvika negativa konsekvenser. 

Induction of Therapeutic T-Cell Responses to Subdominant Tumor-associated Viral Oncogene after Immunization with Replication-incompetent Polyepitope Adenovirus Vaccine

The EBV-encoded latent membrane proteins (LMP1 and LMP2), which are expressed in various EBV-associated malignancies have been proposed as a potential target for CTL-based therapy. However, the precursor frequency for LMP-specific CTL is generally low, and immunotherapy based on these antigens is often compromised by the poor immunogenicity and potential threat from their oncogenic potential. Here we have developed a replication-incompetent adenoviral vaccine that encodes multiple HLA class I-restricted CTL epitopes from LMP1 and LMP2 as a polyepitope. Immunization with this polyepitope vaccine consistently generated strong LMP-specific CTL responses in HLA A2/K-b mice, which can be readily detected by both ex vivo and in vivo T-cell assays. Furthermore, a human CTL response to LMP antigens can be rapidly expanded after stimulation with this recombinant polyepitope vector. These expanded T cells displayed strong lysis of autologous target cells sensitized with LMP1 and/or LMP2 CTL epitopes. More importantly, this adenoviral vaccine was also successfully used to reverse the outgrowth of LMP1-expressing tumors in HLA A2/K-b mice. These studies demonstrate that a replication-incompetent adenovirus polyepitope vaccine is an excellent tool for the induction of a protective CTL response directed toward multiple LMP CTL epitopes restricted through common HLA class I alleles prevalent in different ethnic groups where EBV-associated malignancies are endemic.

Despite differences between dendritic cells and Langerhans cells in the mechanism of papillomavirus-like particle antigen uptake, both cells cross-prime T cells

As human papillomavirus-like particles (HPV-VLP) represent a promising vaccine delivery vehicle, delineation of the interaction of VLP with professional APC should improve vaccine development. Differences in the capacity of VLP to signal dendritic cells (DC) and Langerhans cells (LC) have been demonstrated, and evidence has been presented for both clathrin-coated pits and proteoglycans (PG) in the uptake pathway of VLP into epithelial cells. Therefore, we compared HPV-VLP uptake mechanisms in human monocyte-derived DC and LC, and their ability to cross-present HPV VLP-associated antigen in the MHC class I pathway. DC and LC each took up virus-like particles (VLP). DC uptake of and signalling by VLP was inhibited by amiloride or cytochalasin D (CCD), but not by filipin treatment, and was blocked by several sulfated and non-sulfated polysaccharides and anti-CD16. In contrast, LC uptake was inhibited only by filipin, and VLP in LC were associated with caveolin, langerin, and CD1a. These data suggest fundamentally different routes of VLP uptake by DC and LC. Despite these differences, VLP taken up by DC and LC were each able to prime naive CD8(+) T cells and induce cytolytic effector T cells in vitro. (C) 2004 Elsevier Inc. All rights reserved.

Group A streptococcal vaccine delivery by immunization with a self-adjuvanting M protein-based lipid core peptide construct

Background & objectives: To develop a broad strain coverage GAS vaccine, several strategies have been investigated which included multi-epitope approaches as well as targeting the M protein conserved C-region. These approaches, however, have relied on the use of adjuvants that are toxic for human application. The development of safe and effective adjuvants for human use is a key issue in the development of effective vaccines. In this study, we investigated the lipid polylysine core peptide (LCP) system as a self-adjuvanting GAS vaccine delivery approach. Methods: An LCP-GAS construct was synthesised incorporating multiple copies of a protective peptide epitope (J8) from the conserved carboxy terminal C-repeat region of the M protein. B10.BR mice were immunized parenterally with the LCP-J8 construct, with or without conventional adjuvant, prior to the assessment of immunogenicity and the induction of serum opsonic antibodies. Results: Our data demonstrated immunogenicity of LCP-J8 when coadministered in complete Freund's adjuvant (CFA), or administered in the absence of conventional adjuvant. In both cases, immunization led to the induction of high-titre J8 peptide-specific serum IgG antibody responses, and the induction of heterologous opsonic antibodies that did not cross-react with human heart tissue proteins. Interpretation & conclusion: These data indicated the potential of a novel self-adjuvanting LCP vaccine delivery system incorporating a synthetic GAS M protein C-region peptide immunogen in the induction of broadly protective immune responses, and pointed to the potential application of this system in human vaccine development against infectious diseases.