Piedra negra: estudo de seu agente etiológico em microscopia eletrônica de varredura

No presente trabalho foi realizado estudo em microscopia eletrônica de varredura do fungo Piedraia hortae, através de material obtido de lesões no cabelo de índios do Amazonas. O exame em microscopia eletrônica revelou ascosporos poliédricos, ovóides e arredondados, arranjados isoladamente ou na forma de pseudohifas, de permeio a material extracelular densamente compactado.

Sensors on mobile devices for AAL

Com o envelhecimento da população, as preocupações com a garantia do seu bem-estar aumentam criando a necessidade de desenvolver ferramentas que permitam monitorizar em permanência este sector da população. A utilização de smartphones pelos mais velhos pode ser crucial no seu bem-estar e na sua autonomia contribuindo para a recolha de informação importante já que estes estão muitas vezes equipados com sensores que podem dar indicações preciosas ao cuidador sobre o estado atual do paciente. Os sensores podem fornecer dados sobre a atividade física do paciente, bem como detetar quedas ou calcular a sua posição, com a ajuda do acelerómetro, do giroscópio e do sensor de campo magnético. No entanto, funcionalidades como essas requerem, obrigatoriamente, uma frequência de amostragem mínima por parte dos sensores que permita a implementação de algoritmos, que determinarão esses parâmetros da forma mais exata possível. Dado que nem sempre os pacientes se fazem acompanhar do seu smartphone quando estão na sua residência, a criação de ambientes de AAL (Ambient Assisted Living) com recurso a dispositivos externos que podem ser “vestidos” pelos pacientes pode também ser uma solução adequada. Estes contêm normalmente os mesmos sensores que os smartphones e comunicam com estes através de tecnologias sem fios, como é o caso do Bluetooth Low Energy. Neste trabalho, avaliou-se a possibilidade de alteração da frequência dos sensores em diferentes sistemas operativos, tendo sido efectuadas modificações nas instalações por defeito de alguns sistemas operativos abertos. Com o objectivo de permitir a criação de uma solução de AAL com recurso a um dispositivo externo implementaram-se serviços e perfis num dispositivo externo, o SensorTag.

Space-time monitoring of prescribed burnt soils performance – an effective tool for forest management

Among the most important measures to prevent wild forest fires is the use of prescribed and controlled burning actions in order to reduce the availability of fuel mass. However, the impact of these activities on soil physical and chemical properties varies according to the type of both soil and vegetation and is not fully understood. Therefore, soil monitoring campaigns are often used to measure these impacts. In this paper we have successfully used three statistical data treatments - the Kolmogorov-Smirnov test followed by the ANOVA and the Kruskall-Wallis tests – to investigate the variability among the soil pH, soil moisture, soil organic matter and soil iron variables for different monitoring times and sampling procedures.

Low-cost vibration sensors: tendencies and applications in condition monitoring of machines and structures

Trabalho Final de Mestrado para obtenção do grau de Mestre em Engenharia Mecânica

Soil sampling design and sampling techniques for soil properties monitoring – common difficulties related to forest soils

Mathematical models and statistical analysis are key instruments in soil science scientific research as they can describe and/or predict the current state of a soil system. These tools allow us to explore the behavior of soil related processes and properties as well as to generate new hypotheses for future experimentation. A good model and analysis of soil properties variations, that permit us to extract suitable conclusions and estimating spatially correlated variables at unsampled locations, is clearly dependent on the amount and quality of data and of the robustness techniques and estimators. On the other hand, the quality of data is obviously dependent from a competent data collection procedure and from a capable laboratory analytical work. Following the standard soil sampling protocols available, soil samples should be collected according to key points such as a convenient spatial scale, landscape homogeneity (or non-homogeneity), land color, soil texture, land slope, land solar exposition. Obtaining good quality data from forest soils is predictably expensive as it is labor intensive and demands many manpower and equipment both in field work and in laboratory analysis. Also, the sampling collection scheme that should be used on a data collection procedure in forest field is not simple to design as the sampling strategies chosen are strongly dependent on soil taxonomy. In fact, a sampling grid will not be able to be followed if rocks at the predicted collecting depth are found, or no soil at all is found, or large trees bar the soil collection. Considering this, a proficient design of a soil data sampling campaign in forest field is not always a simple process and sometimes represents a truly huge challenge. In this work, we present some difficulties that have occurred during two experiments on forest soil that were conducted in order to study the spatial variation of some soil physical-chemical properties. Two different sampling protocols were considered for monitoring two types of forest soils located in NW Portugal: umbric regosol and lithosol. Two different equipments for sampling collection were also used: a manual auger and a shovel. Both scenarios were analyzed and the results achieved have allowed us to consider that monitoring forest soil in order to do some mathematical and statistical investigations needs a sampling procedure to data collection compatible to established protocols but a pre-defined grid assumption often fail when the variability of the soil property is not uniform in space. In this case, sampling grid should be conveniently adapted from one part of the landscape to another and this fact should be taken into consideration of a mathematical procedure.

Entre o palco e o ecrã: feras amestradas: comédia musical negra à portuguesa: intermédia

Trabalho de Projeto submetido à Escola Superior de Teatro e Cinema para cumprimento dos requisitos necessários à obtenção do grau de Mestre em Teatro - especialização em Encenação

Análise polinica de argilas de Lagoa Negra

Palynological analysis of black shales from Lagoa Negra (Cantanhede) shows the presence of Cathaya and Keteleeria, Myrica and Engelhardtia being scarce. Comparison with other localities suggest an Upper Pliocene or Lower Pleistocene age for this association.

Environmental indicator frameworks to design and assess environmental monitoring programs

Impact Assessment and Project Appraisal, vol. 22, n.1, March 2004, p. 47–62

Assessment of nutritional and metabolic profiles of pea shoots: The new ready-to-eat baby-leaf vegetable

Pea-shoots are a new option as ready-to-eat baby-leaf vegetable. However, data about the nutritional composition and the shelf-life stability of these leaves, especially their phytonutrient composition is scarce. In this work, the macronutrient, micronutrient and phytonutrients profile of minimally processed pea shoots were evaluated at the beginning and at the end of a 10-day storage period. Several physicochemical characteristics (color, pH, total soluble solids, and total titratable acidity) were also monitored. Standard AOAC methods were applied in the nutritional value evaluation, while chromatographic methods with UV–vis and mass detection were used to analyze free forms of vitamins (HPLC-DAD-ESI-MS/MS), carotenoids (HPLC-DAD-APCI-MSn) and flavonoid compounds (HPLC-DAD-ESI-MSn). Atomic absorption spectrometry (HR-CS-AAS) was employed to characterize the mineral content of the leaves. As expected, pea leaves had a high water (91.5%) and low fat (0.3%) and carbohydrate (1.9%) contents, being a good source of dietary fiber (2.1%). Pea shoots showed a high content of vitamins C, E and A, potassium and phosphorous compared to other ready-to-eat green leafy vegetables. The carotenoid profile revealed a high content of β-carotene and lutein, typical from green leafy vegetables. The leaves had a mean flavonoid content of 329 mg/100 g of fresh product, mainly composed by glycosylated quercetin and kaempferol derivatives. Pea shoots kept their fresh appearance during the storage being color maintained throughout the shelf-life. The nutritional composition was in general stable during storage, showing some significant (p < 0.05) variation in certain water-soluble vitamins.

Toarcian GSSP candidate: the Peniche section at Ponta do Trovão

The Peniche section (Ponta do Trovão) in Portugal is presented as potential stratotype (GSSP) for the Pliensbachian/Toarcian boundary. The lithostratigraphic succession is described and the chronostratigraphy, based on ammonite assemblages, is presented; the change in foraminifera assemblages occurs later, only at the base of beds 16 (base of Semicelatum Subzone, Crosbeyi ? Horizon). An extensive bibliographical list of all scientific articles containing specific reference to this stratigraphic boundary, whether from the Lusitanian or Algarve basins, is also presented.

Calcareous nannofossil assemblages across the Pliensbachian/Toarcian boundary at the Peniche section (Ponta do Trovão, Lusitanian Basin)

The Peniche section has revealed moderately-to-well preserved calcareous nannofossil assemblages across the Pliensbachian/Toarcian boundary. This good record has allowed the proposition of a refined biostratigraphic scheme. The stage boundary, as defined by ammonites, is comprised within the NJ5b C. impontus (NW Europe; BOWN & COOPER, 1998) or the NJT5b L. sigillatus (Mediterranean Tethys; MATTIOLI & ERBA, 1999) nannofossil subzones. Since in the Lusitanian Basin a mixing of N- and S-Tethyan taxa is observed, both biozonation schemes can be applied. Some nannofossil events (mainly first occurrences) are observed earlier in Portugal than in other Tethyan settings. It is still unclear if these events are real first occurrences. A diversification phase occurred across the Pliensbachian/Toarcian boundary. This phase is well recorded at Peniche, where a change is observed passing from the Pliensbachian, when assemblages are dominated by muroliths, to the Toarcian showing assemblages where placoliths are abundant. A quantification of nannofossils per gram of rock shows that absolute abundances are the highest across the Pliensbachian/Toarcian boundary. Indeed, Peniche exhibits nannofossil abundances very high with respect to correlative levels in other Tethyan settings. The pelagic carbonate fraction (produced by nannofossils) is important in the marly hemi-couplets of Peniche. In some levels, nannofossils account for more than 50% of the total carbonate fraction.

Supporting On-Line Distributed Monitoring and Debugging

Monitoring systems have traditionally been developed with rigid objectives and functionalities, and tied to specific languages, libraries and run-time environments. There is a need for more flexible monitoring systems which can be easily adapted to distinct requirements. On-line monitoring has been considered as increasingly important for observation and control of a distributed application. In this paper we discuss monitoring interfaces and architectures which support more extensible monitoring and control services. We describe our work on the development of a distributed monitoring infrastructure, and illustrate how it eases the implementation of a complex distributed debugging architecture. We also discuss several issues concerning support for tool interoperability and illustrate how the cooperation among multiple concurrent tools can ease the task of distributed debugging.

The Farol Deposit (Depósito do Farol) – a Pleistocene beach deposit from Cape Mondego (Figueira da Foz, West Central Portugal)*

Among the Pleistocene and Holocene units recorded near the marine cliffs of Cape Mondego (Figueira da Foz, West Central Portugal) stands out the Farol Deposit (Depósito do Farol), at an altitude of ±95 m above present sea level. It is a marine terrace with three exposures of interstratified conglomerates and sands, overlapped by calclititic-fanglomerates. This sedimentary setting indicates that deposition took place in a seashore environment influenced by the proximity of a marine palaeocliff. The deposit has an interesting subfossil fauna with abraded and fragmented shells of Nucella lapillus (LINNÉ, 1758), Patella vulgata (LINNÉ, 1758) and Littorina littorea (LINNÉ, 1758), suggesting the existence of an environment with colder surface seawater, when compared with the present day Portuguese seashore. These specimens belonged to marine communities adapted to live in intertidal rocky platforms, which have been exposed to the cyclic action of waves and tidal flows, on the swash and surf zones. The Farol Deposit can be related to an Early/Middle Pleistocene “cold-water” episode, earlier to the Isotopic Stages 7 and 11. This episode occurred before the deposition of the units Quiaios Sands (Areias de Quiaios) and Cantanhede Sands (Areias de Cantanhede) (Sicilian?), but later than the Arazede Sands (Areias de Arazede) and Marinha das Ondas Sands (Areias de Marinha das Ondas) (Early Pleistocene).

Planning smart cities using wireless low energy monitoring systems

Thesis to obtain the Master Degree in Electronics and Telecommunications Engineering

Development of a high-throughput monitoring technique of bacteria photodynamic inactivation

Summary form only given. Bacterial infections and the fight against them have been one of the major concerns of mankind since the dawn of time. During the `golden years' of antibiotic discovery, during the 1940-90s, it was thought that the war against infectious diseases had been won. However currently, due to the drug resistance increase, associated with the inefficiency of discovering new antibiotic classes, infectious diseases are again a major public health concern. A potential alternative to antibiotic treatments may be the antimicrobial photodynamic inactivation (PDI) therapy. To date no indication of antimicrobial PDI resistance development has been reported. However the PDI protocol depends on the bacteria species [1], and in some cases on the bacteria strains, for instance Staphylococcus aureus [2]. Therefore the development of PDI monitoring techniques for diverse bacteria strains is critical in pursuing further understanding of such promising alternative therapy. The present works aims to evaluate Fourier-Transformed-Infra-Red (FT-IR) spectroscopy to monitor the PDI of two model bacteria, a gram-negative (Escherichia coli) and a gram-positive (S. aureus) bacteria. For that a high-throughput FTIR spectroscopic method was implemented as generally described in Scholz et al. [3], using short incubation periods and microliter quantities of the incubation mixture containing the bacteria and the PDI-drug model the known bactericidal tetracationic porphyrin 5,10,15,20-tetrakis (4-N, N, Ntrimethylammoniumphenyl)-porphyrin p-tosylate (TTAP4+). In both bacteria models it was possible to detect, by FTIR-spectroscopy, the drugs effect on the cellular composition either directly on the spectra or on score plots of principal component analysis. Furthermore the technique enabled to infer the effect of PDI on the major cellular biomolecules and metabolic status, for example the turn-over metabolism. In summary bacteria PDI was monitored in an economic, rapid (in minutes- , high-throughput (using microplates with 96 wells) and highly sensitive mode resourcing to FTIR spectroscopy, which could serve has a technological basis for the evaluation of antimicrobial PDI therapies efficiency.