Diseño y caracterización de sistemas nanoestructurados a partir de copolímeros SBM

215 p.

Biochemical biomarkers in samples processed by different methods in Environmental Specimen Banks

In the last decades the creation of new Environmental Specimen Banks (ESB) is increasing due to the necessity of knowing the effects of pollutants in both the environment and human populations. ESBs analyze and store samples in order to understand the effects of chemicals, emerging substances and the environmental changes in biota. For a correct analysis of the effect induced by these variables, there is a need to add biological endpoints, such as biomarkers, to the endpoints based on chemical approaches which have being used until now. It is essential to adapt ESB´s sampling strategies in order to enable scientists to apply new biological methods. The present study was performed to obtain biochemical endpoints from samples stored in the BBEBB (Biscay Bay Environmental Biospecimen Bank) of the Marine Station of Plentzia (PIE - UPV/EHU). The main objective of the present work was to study the variability caused in biochemical biomarkers by different processing methods in mussels (Mytilus galloprovincialis) from two localities (Plentzia and Arriluze) with different pollution history. It can be concluded that the selected biomarkers (glutathione S-transferase and acetylcholinesterase) can be accurately measured in samples stored for years in the ESBs. The results also allowed the discrimination of both sampling sites. However, in a further step, the threshold levels and baseline values should be characterized for a correct interpretation of the results in relation to the assessment of the ecosystem health status.

Hediste diversicolor itsas poliketoaren azterketa biokimiko eta histologikoa

[EUS] Hediste diversicolor poliketo espeziearekin burututako eta dietarekin lotutako azterketa esperimental bat azaltzen da. Baita poliketoaren populazio basati batean eta haztegiko populazioan egindako azterketa biokimiko eta histologikoak eta hauen arteko desberdintasunak.

Identification of treatments targeting PMLexpressing breast cancers

The main aim of this project is to find a suitable ATO concentration to combine with already approved chemotherapeutic agents to find that synergistic effect in triple negative breast cancer MDA-MB 231 cell line, as a new strategy to treat the disease.

Implicación de los microRNAs en la toxicidad del metotrexato en Leucemia Linfoblástica Aguda infantil

Analisis de la implicacion de los Polimorfismos de LLA en la respuesta al Metotrexato

Implicación de rs924607 del gen CEP72 en la neurotoxicidad asociada a la vincristina en pacientes con leucemia linfoblastica aguda (LLA)

Este trabajo se trata de un estudio estadístico de asociación entre el genotipo del SNP rs924607 presente en la región promotora del gen CEP72, y la neurotoxicidad asociada a la vincristina, en pacientes pediátricos de Leucemia Linfoblástica Aguda. El objetivo es determinar si el alelo presente en dicho SNP puede ser empleado como biomarcador para predecir la neurotoxicidad asociada a la vincristina.

Silenciamiento de PTPRK, un gen candidato de la enfermedad celíaca

La enfermedad celíaca, también conocida como enteropatía sensible al gluten, es un trastorno autoinmune crónico causada por la intolerancia a la ingesta de gluten y desarrollada por personas genéticamente predispuestas cuyo único tratamiento consiste en una dieta estricta libre de gluten. El desarrollo de dicha enfermedad está relacionada con una gran variedad de genes entre los que se encuentra PTPRK. Este trabajo desarrolla un estudio de la expresión de dicho gen así como un protocolo de silenciamiento del mismo.

Variantes genéticas en ANRIL y longevidad

[ES] En este trabajo se ha analizado el efecto de 4 tag-SNPs localizados en la región promotora del ARN no codificante ANRIL en la población longeva. Se cree que la desregulación de ANRIL podría estar asociada a la longevidad, considerando que esta desregulación podría ser debida a variantes genéticas en la región promotora del mismo. Idioma: Español.

Zonas de Brillouin de los grupos de capa

La base de datos de las zonas de Brillouin de los grupos de capa del Bilbao Crystallographic Server incluye tablas de vectores de onda y figuras que forman la base para la clasificación de las representaciones de los grupos de capa. Las propiedades de simetría de los vectores de onda se determinan por los llamados grupos del espacio recíproco y esta clasificación se compara con la que recoge el libro “Character Tables and Compatibility Relations of The Eighty Layer Groups and Seventeen Plane Groups” de Litvin & Wike.

Genetic Variants in MiRNA Processing Genes and Pre-MiRNAs Are Associated with the Risk of Chronic Lymphocytic Leukemia

Genome wide association studies (GWAS) have identified several low-penetrance susceptibility alleles in chronic lymphocytic leukemia (CLL). Nevertheless, these studies scarcely study regions that are implicated in non-coding molecules such as microRNAs (miRNAs). Abnormalities in miRNAs, as altered expression patterns and mutations, have been described in CLL, suggesting their implication in the development of the disease. Genetic variations in miRNAs can affect levels of miRNA expression if present in pre-miRNAs and in miRNA biogenesis genes or alter miRNA function if present in both target mRNA and miRNA sequences. Therefore, the present study aimed to evaluate whether polymorphisms in pre-miRNAs, and/or miRNA processing genes contribute to predisposition for CLL. A total of 91 SNPs in 107 CLL patients and 350 cancer-free controls were successfully analyzed using TaqMan Open Array technology. We found nine statistically significant associations with CLL risk after FDR correction, seven in miRNA processing genes (rs3805500 and rs6877842 in DROSHA, rs1057035 in DICER1, rs17676986 in SND1, rs9611280 in TNRC6B, rs784567 in TRBP and rs11866002 in CNOT1) and two in pre-miRNAs (rs11614913 in miR196a2 and rs2114358 in miR1206). These findings suggest that polymorphisms in genes involved in miRNAs biogenesis pathway as well as in pre-miRNAs contribute to the risk of CLL. Large-scale studies are needed to validate the current findings.

Filosofia com crianças: dos saberes da infância à infância dos saberes

Este é um texto que se constrói sobre uma linha frágil, contraditória talvez. Acredita na impossibilidade de transmitir uma experiência narrada, mas que, ao mesmo tempo, tenta fazer-se experiência em certo tipo de narração. Um desafio; um risco que corremos ao escrever sobre os efeitos das práticas de filosofia com crianças da Escola Municipal Pedro Rodrigues do Carmo, de Duque de Caxias, RJ,envolvidas com o projeto de extensão universitária 'Em Caxias, a filosofia em-caixa?A escola pública aposta no pensamento', coordenada pelo professor Walter Omar Kohan e desenvolvido pelo Núcleo de Estudos Filosóficos da Infância (NEFI) da Universidade do Estado do Rio de Janeiro (UERJ). Como professor envolvido comeste projeto desde 2009, pude notar alguns aspectos nestas práticas que apontavam indícios de que uma outra relação com os tempos, os saberes e as infâncias eram possíveis na complexidade cotidiana de uma escola. Tomando tais indícios como pistas de uma investigação, buscou-se compreender como estes temas se relacionavam com a escola e com os sujeitos formados por ela. A opção estética para este texto foi apresentá-lo como uma experiência de filosofia, partindo de uma 'disposição inicial', onde se apresenta de maneira leve as propostas deste escrito;passando pela 'vivência de um texto', onde apresentar-se-á o projeto 'Em Caxias, a filosofia em-caixa?' como texto a ser experienciado; rumando para uma 'problematização', de onde se levantam questões acerca deste texto; passando para a 'escolha de questões' a serem abordadas; indo, pois, para o 'diálogo' com os autores acerca dos temas eleitos e finalizando com um momento que chamamos de 'para continuar pensando', que de maneira alguma representa o fim derradeiro de uma questão. Para este diálogo, foram convidados W. Kohan e suas percepções sobre a infância, M. Foucault e suas contribuições sobre as relações de saber/poder,e, a partir dele, se estabelece uma relação com Jorge Larrosa que se propõe a discutir a formação do sujeito e as possibilidades da filosofia e da educação.Partindo de referenciais teóricos distintos, mas encontrando pontos de convergências com estas discussões, temos o harendtiano Jan Masschelein e suas considerações sobre a profanação e a suspensão dos saberes e a dissonante voz de Jacques Rancière e suas proposições sobre emancipação e embrutecimento. A última parte deste escrito consiste nas considerações finais acerca das discussões e a ponta algumas possibilidades de construção de um espaço/tempo onde as relações entre sujeitos e saberes pode se dar de uma outra maneira.

Double layers in gravity theories

Gravitational double layers, unlike their classical electromagnetic counterparts, are thought to be forbidden in gravity theories. It has been recently shown, however, that they are feasible in, for instance, gravity theories with a Lagrangian quadratic in the curvature. This is surprising with many potential consequences and the possibility of new physical behaviours. While a clear interpretation seems elusive, several lines of research are open. I present the field equations for double layers, the new physical quantities arising, and several explicit examples

The center of mass and center of charge of the electron

9th Biennial Conference on Classical and Quantum Relativistic Dynamics of Particles and Fields (IARD)

Pharmacological Blockade of Cannabinoid CB1 Receptors in Diet-Induced Obesity Regulates Mitochondrial Dihydrolipoamide Dehydrogenase in Muscle

Cannabinoid CB1 receptors peripherally modulate energy metabolism. Here, we investigated the role of CB1 receptors in the expression of glucose/pyruvate/tricarboxylic acid (TCA) metabolism in rat abdominal muscle. Dihydrolipoamide dehydrogenase (DLD), a flavoprotein component (E3) of alpha-ketoacid dehydrogenase complexes with diaphorase activity in mitochondria, was specifically analyzed. After assessing the effectiveness of the CB1 receptor antagonist AM251 (3 mg kg(-1), 14 days) on food intake and body weight, we could identified seven key enzymes from either glycolytic pathway or TCA cycle-regulated by both diet and CB1 receptor activity-through comprehensive proteomic approaches involving two-dimensional electrophoresis and MALDI-TOF/LC-ESI trap mass spectrometry. These enzymes were glucose 6-phosphate isomerase (GPI), triosephosphate isomerase (TPI), enolase (Eno3), lactate dehydrogenase (LDHa), glyoxalase-1 (Glo1) and the mitochondrial DLD, whose expressions were modified by AM251 in hypercaloric diet-induced obesity. Specifically, AM251 blocked high-carbohydrate diet (HCD)-induced expression of GPI, TPI, Eno3 and LDHa, suggesting a down-regulation of glucose/pyruvate/lactate pathways under glucose availability. AM251 reversed the HCD-inhibited expression of Glo1 and DLD in the muscle, and the DLD and CB1 receptor expression in the mitochondrial fraction. Interestingly, we identified the presence of CB1 receptors at the membrane of striate muscle mitochondria. DLD over-expression was confirmed in muscle of CB1-/- mice. AM251 increased the pyruvate dehydrogenase and glutathione reductase activity in C2C12 myotubes, and the diaphorase/oxidative activity in the mitochondria fraction. These results indicated an up-regulation of methylglyoxal and TCA cycle activity. Findings suggest that CB1 receptors in muscle modulate glucose/pyruvate/lactate pathways and mitochondrial oxidative activity by targeting DLD.