RGS-GAIP, a GTPase-activating Protein for Gαi Heterotrimeric G Proteins, Is Located on Clathrin-coated Vesicles

RGS-GAIP (Gα-interacting protein) is a member of the RGS (regulator of G protein signaling) family of proteins that functions to down-regulate Gαi/Gαq-linked signaling. GAIP is a GAP or guanosine triphosphatase-activating protein that was initially discovered by virtue of its ability to bind to the heterotrimeric G protein Gαi3, which is found on both the plasma membrane (PM) and Golgi membranes. Previously, we demonstrated that, in contrast to most other GAPs, GAIP is membrane anchored and palmitoylated. In this work we used cell fractionation and immunocytochemistry to determine with what particular membranes GAIP is associated. In pituitary cells we found that GAIP fractionated with intracellular membranes, not the PM; by immunogold labeling GAIP was found on clathrin-coated buds or vesicles (CCVs) in the Golgi region. In rat liver GAIP was concentrated in vesicular carrier fractions; it was not found in either Golgi- or PM-enriched fractions. By immunogold labeling it was detected on clathrin-coated pits or CCVs located near the sinusoidal PM. These results suggest that GAIP may be associated with both TGN-derived and PM-derived CCVs. GAIP represents the first GAP found on CCVs or any other intracellular membranes. The presence of GAIP on CCVs suggests a model whereby a GAP is separated in space from its target G protein with the two coming into contact at the time of vesicle fusion.

Chicago, Illinois, railroads and industry, 1886 (Image 2 of 2) (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Railway terminal and industrial map of Chicago : showing the termini, connections, and general system by which interchanges and transfers of freights are effected between all railroads centering in and about Chicago, also indicating the location of freight and passenger depots, elevators, warehouses, coal, ore, and other docks, and the leading manufactories, with an alphabetical list of the principle industries located along the lines of the same, drawn & engraved by A. Zecse & Co. It was published by Industrial World. Co. in 1886. Scale [ca. 1:19,300]. This layer is image 2 of 2 total images of the two sheet source map, representing the southern portion of the map. The image inside the map neatline is georeferenced to the surface of the earth and fit to the Illinois East State Plane Coordinate System NAD83 (in Feet) (Fipszone 1201). All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as roads, railroads, drainage, selected buildings and industry locations (e.g. mills, factories, etc.), and more. Includes index of principal industries. This layer is part of a selection of digitally scanned and georeferenced historic maps from The Harvard Map Collection as part of the Imaging the Urban Environment project. Maps selected for this project represent major urban areas and cities of the world, at various time periods. These maps typically portray both natural and manmade features at a large scale. The selection represents a range of regions, originators, ground condition dates, scales, and purposes.

Chicago, Illinois, railroads and industry, 1886 (Image 1 of 2) (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Railway terminal and industrial map of Chicago : showing the termini, connections, and general system by which interchanges and transfers of freights are effected between all railroads centering in and about Chicago, also indicating the location of freight and passenger depots, elevators, warehouses, coal, ore, and other docks, and the leading manufactories, with an alphabetical list of the principle industries located along the lines of the same, drawn & engraved by A. Zecse & Co. It was published by Industrial World. Co. in 1886. Scale [ca. 1:19,300]. This layer is image 1 of 2 total images of the two sheet source map, representing the northern portion of the map. The image inside the map neatline is georeferenced to the surface of the earth and fit to the Illinois East State Plane Coordinate System NAD83 (in Feet) (Fipszone 1201). All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as roads, railroads, drainage, selected buildings and industry locations (e.g. mills, factories, etc.), and more. Includes index of principal industries. This layer is part of a selection of digitally scanned and georeferenced historic maps from The Harvard Map Collection as part of the Imaging the Urban Environment project. Maps selected for this project represent major urban areas and cities of the world, at various time periods. These maps typically portray both natural and manmade features at a large scale. The selection represents a range of regions, originators, ground condition dates, scales, and purposes.

Addis Ababa Region, Ethiopia, 1886 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Le regioni a libeccio dello Scioa occupate da Re Menilek : secondo l'itinerario del Cap. A. Cecchi e dell'Ing. G. Chiarini costrutto nel 1883 dal Cap. Antonio Cecchi ; disegnato dall'Ing. R. Mengaroni. It was published by Società geografica italiana in 1886. Scale 1:1,000,000. Covers the Addis Ababa, Shewa region, Ethiopia. Map in Italian.The image inside the map neatline is georeferenced to the surface of the earth and fit to the World Miller Cylindrical projected coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, cities and other human settlements, territorial boundaries, forests, and more. Shows the travel routes of Antonio Cecchi and Giovanni Chiarini, 1878-1879. Relief shown by hachures and spot heights.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

East Bridgewater, Massachusetts, 1848 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Map of East Bridgewater, Mass., surveyed by M. Bates, Jr., 1848. It was published by J.H. Bufford's Lithography in 1848. Scale [1:19,800]. Covers also portions of Whitman and Brockton, Massachusetts. The image inside the map neatline is georeferenced to the surface of the earth and fit to the Massachusetts State Plane Coordinate System, Mainland Zone (in Feet) (Fipszone 2001). All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as roads, railroads, drainage, public buildings, schools, churches, cemeteries, industry locations (e.g. mills, factories, mines, etc.), private buildings with names of property owners, town and district boundaries and more. Relief is shown by hachures.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

Analyzation of marine snow and fecal pellets collected in sediment trap CBi-2 off Cape Blanc, Mauritania

We analyzed size-specific dry mass, sinking velocity, and apparent diffusivity in field-sampled marine snow, laboratory-made aggregates formed by diatoms or coccolithophorids, and small and large zooplankton fecal pellets with naturally varying content of ballast materials. Apparent diffusivity was measured directly inside aggregates and large (millimeter-long) fecal pellets using microsensors. Large fecal pellets, collected in the coastal upwelling off Cape Blanc, Mauritania, showed the highest volume-specific dry mass and sinking velocities because of a high content of opal, carbonate, and lithogenic material (mostly Saharan dust), which together comprised ~80% of the dry mass. The average solid matter density within these large fecal pellets was 1.7 g cm**-3, whereas their excess density was 0.25 ± 0.07 g cm**-3. Volume-specific dry mass of all sources of aggregates and fecal pellets ranged from 3.8 to 960 µg mm**-3, and average sinking velocities varied between 51 and 732 m d**-1. Porosity was >0.43 and >0.96 within fecal pellets and phytoplankton-derived aggregates, respectively. Averaged values of apparent diffusivity of gases within large fecal pellets and aggregates were 0.74 and 0.95 times that of the free diffusion coefficient in sea water, respectively. Ballast increases sinking velocity and, thus, also potential O2 fluxes to sedimenting aggregates and fecal pellets. Hence, ballast minerals limit the residence time of aggregates in the water column by increasing sinking velocity, but apparent diffusivity and potential oxygen supply within aggregates are high, whereby a large fraction of labile organic carbon can be respired during sedimentation.

La fraternidad uruguaya. El acuerdo electoral [por] C.M. Ramírez, J.G. Busto [y] Byzantinus.

Mode of access: Internet.

Graham's American monthly magazine of literature, art, and fashion.

Editors: Jan. 1841- G. R. Graham (with E. A. Poe, Jan. 1841-May 1842; C. J. Peterson, Mrs. Ann S. Stephens, 1842; R. W. Griswold, July 1842-June 1843; R. T. Conrad, Jan.-June 1848: J. R. Chandler, J. B. Taylor, Oct. 1848-Dec. 1849).

Die schmetterlingsfauna Nordwest-Deutschlands : insbesondere die lepidopterologischen verhältnisse der umgebung von Göttingen /

"Litteratur": p. [xiii]-xviii.

Shaker almanac, 1886 : the mystery explained.

Almanac features biographies and portraits of American millionaires.

Concerto, in G minor, for violin and piano, op. 26 /

"Carl Fischer's music library, no. 312."

Concerto no. 4 in G : (Concertstück) : for cello and piano, op. 65 /

Mode of access: Internet.

Modern trio album. [music] : fifteen special concert arrangements and original compositions for violin, cello and piano /

Elegie and consolation, op. 200, no. 2 / Atherton, Frank P. -- Hungarian dance no. 5 (in G minor) / Brahms, Johannes -- Humoreske : op. 101, no. 7 / Dvor̂ák, Anton ; arr. by Gustav Saenger -- La Cinquantaine / Gabriel-Marie ; special concert arr. by C. Arnold -- Gavotte Louis XIII : Amaryllis / Ghys, H. ; arr. by N. Hansen -- Serenade, from Op. 3 / Herbert, Victor -- Dreams of love = Liebesträume : notturno III / Liszt, Franz ; arr. by R. Klugescheid -- O Belle Nuite, Barcarolle from "Contes d'Hoffmann" / Offenbach, J. ; arr. by Max Woltag -- Celebrated Andante / Reissiger, C. G. -- My heart at thy sweet voice : Cantabile from "Samson and Dalila" / Saint-Saëns, C. ; arr. by R. Klugescheid -- Melodie Mignonne / Sinding, Christian ; arr. by N. Hansen -- Vienna life = Wiener Blut / Strauss, Johann ; special concert arr. by C. Arnold -- Autumn song = Chant d'automne ; Chant sans paroles : op. 40, no. 6 / Tschaikowsky, P. ; arr. by N. Hansen -- Song to the evening star : from "Tannhäuser" / Wagner, Richard ; concert paraphrase by C. Arnold.