Determinação e diferenciação de castas em Mischocyttarus (Mischocyttarus) drewseni Saussure, 1857 (Hymenoptera, Vespidae) de acordo com a perspectiva nutricional e aspectos relacionados

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Morfofisiologia das castas e forrageamento do cupim de cerrado Velocitermes heteropterus (Isoptera: Termitidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Aspectos bioecológicos de Angiopolybia pallens (Lepeletier, 1836) (Hymenoptera-Vespidae)

Pós-graduação em Ciências Biológicas (Zoologia) - IBRC

Efeito da cafeína no comportamento e na longevidade de operárias de abelhas africanizadas Apis mellifera L. (Hymenoptera: Apidae)

Pós-graduação em Ciências Biológicas (Zoologia) - IBRC

Impacto do uso droga imunomoduladora no tratamento da artrite séptica por Staphylococcus aureus

Pós-graduação em Doenças Tropicais - FMB

Interações entre diferentes espécies de Candida na candidose experimental em modelos de invertebrados e vertebrados

Pós-graduação em Biopatologia Bucal - ICT

Serum bactericidal activity as indicator of innate immunity in pacu Piaractus mesopotamicus (Holmberg, 1887)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Culture of equine bone marrow mononuclear fraction and adipose tissue-derived stromal vascular fraction cells in different media

The objective of this study was to evaluate the culture of equine bone marrow mononuclear fraction and adipose tissue - derived stromal vascular fraction cells in two different cell culture media. Five adult horses were submitted to bone marrow aspiration from the sternum, and then from the adipose tissue of the gluteal region near the base of the tail. Mononuclear fraction and stromal vascular fraction were isolated from the samples and cultivated in DMEM medium supplemented with 10% fetal bovine serum or in AIM-V medium. The cultures were observed once a week with an inverted microscope, to perform a qualitative analysis of the morphology of the cells as well as the general appearance of the cell culture. Colony-forming units (CFU) were counted on days 5, 15 and 25 of cell culture. During the first week of culture, differences were observed between the samples from the same source maintained in different culture media. The number of colonies was significantly higher in samples of bone marrow in relation to samples of adipose tissue.

Epidemiologia molecular aplicada ao monitoramento de estirpes de Staphylococcus aureus na produção de queijo Minas frescal

We studied the molecular epidemiology of Staphylococcus aureus strains potentially toxigenic, isolated from the production process of Minas frescal cheese in a small dairy plant in the state of São Paulo. For this, samples were taken during the period from June 2008 to July 2009. Samples were collected from the surface of the receiving and storage tanks of raw milk, the surface of the balance tank of pasteurized milk, the water supply system, the pipes and equipments, the hands of the handler and from the packaged cheese, totaling 140 samples. The colonies isolated on Baird-Parker Agar confirmed as Gram positive and positive for catalase, coagulase and acetoin production, were submitted to extraction of bacterial DNA using the Invitek - Uniscience® kit. Confirmation of the isolated species and enterotoxins SEA, SEB, SEC, SED and TSST-1 toxin was carried out through the amplification of specific fragments of chromosomal DNA. Among the 74 strains of isolated coagulase-positive staphylococci, only 41 (55.4%) strains were confirmed as Staphylococcus aureus, of which 25 (61.0%) were positive to the presence of staphylococcal toxins. The most frequently identified enterotoxin was SEA. The toxigenic strains of Staphylococcus aureus were more frequently isolated from hands of the handler (16.0%), raw milk receiving tank (12.0%), pasteurized milk for cheese making (12.0%) and fresh white cheese ready for consumption (12.0%).

Avaliação de fungicidas, óleos essenciais e agentes biológicos no controle de Amphobotrys ricini em mamoneira (Ricinus communis L.)

The castor bean (Ricinus communis L.) is a tropical oilseed species, and the oil extracted from its seeds is one of the most versatile oils in the nature, showing various industrial uses. Even though it is a rustic species, the castor bean is subjected to several diseases such as the gray mold, caused by the fungus Amphobotrys ricini. Genetic breeding would be the best alternative for the disease control, but a long time is required to obtain resistant cultivars. Thus, the use of control strategies based on chemical, alternative or biological methods shows viable in the short term. The aim of this study was to investigate gray mold control efficiency, in castor bean crop, using chemical, alternative and biological methods. The pathogen control efficiency was evaluated both in vitro and in vivo using fungicides, essential oils and biological control agents. As regards the in vitro inhibition of the pathogen mycelial growth, the best treatments with essential oils were those based on C. martini and C. zeylanicum at all five tested concentrations. For both oils, the average diameter of colonies was 0.7 cm against 4.79 cm for the control treatment. For the fungicides, at all four tested levels, the most efficient active ingredients were methyl tiophanate, carbendazim, tebuconazole and iprodione. The ED50 of these fungicides was <1uL/L, yielding 100% mycelial growth inhibition at all concentrations. As to the inhibition of A. ricini conidium germination, the fungicides tebuconazole and chlorotanolyl were the best at all tested concentrations, and the average of germinated conidia with these fungicides was 0.0 and 0.15%, respectively, against 100% for the control treatment. In the field, treatment with the fungicide iprodione was the best for the disease control when compared to biological and alternative treatments. Under field conditions, the average disease severity for the treatment with iprodione was 15.76% against 95.81% for the inoculated control.

Mycelial growth of two Lentinula edodes strains in culture media prepared with sawdust extracts from seven eucalyptus species and three eucalyptus clones

The in vitro mycelial growth of Lentinula edodes strains LE-95/01 and LE-96/18 were evaluated in solid culture media prepared with sawdust extracts from seven eucalyptus species (E. saligna, E. grandis, E. urophylla, E. pellita, E. paniculata, E. citriodora, and E. camaldulensis) and three eucalyptus clones (E. grandis × E. urophylla hybrids). Evaluations were made every 48 hours by means of colony diameter measurements (mean of four transversely-oriented measurements), during ten days of incubation in the dark at 25ºC ±1°C. The experimental design consisted of randomized blocks, and treatment means were compared by Tukey test. The culture medium prepared from E. citriodora sawdust extract was the most promising to grow L. edodes strains LE-96/18 and LE-95/01. L. edodes strain LE-96/18 presented the fastest mycelial growth after incubation for ten days, regardless of sawdust extract type used in the culture medium.

Efeito do tratamento local de vesiculite seminal sobre a qualidade e longevidade so sêmen equino

Pós-graduação em Medicina Veterinária - FMVZ

Ocorrência e identificação molecular de espécies do gênero Mycobacterium e marcadores de virulência em linhagens de Rhodococcus equi isoladas de linfonodos e das fezes de suínos de abatedeouro

Pós-graduação em Medicina Veterinária - FMVZ

Caracterização funcional do produto da ORF NCU03043 de Neurospora crassa homólogo ao fator de transcrição FlbC de Aspergillus nidulans

Pós-graduação em Biotecnologia - IQ

Determinação das estratégias coletivas de forrageamento em Atta sexdens rubropilosa (Hymenoptera, Formicidae)

Pós-graduação em Agronomia (Proteção de Plantas) - FCA