Perfil de vitamina D e sua associação com adiposidade corporal e resistência à insulina em pacientes com doença renal crônica na fase não dialítica

O termo vitamina D compreende um grupo de hormônios esteróides com ações biológicas semelhantes. O método mais acurado para determinar o estado de vitamina D é através dos níveis plasmáticos de 25 hidroxivitamina D [25(OH)D]. A deficiência de 25(OH)D é considerada um problema de saúde pública, tendo como principal causa à baixa exposição solar, idade avançada e doenças crônicas. A deficiência de 25(OH)D é frequente em pacientes com doença renal crônica (DRC) na fase não dialítica. Estudos têm evidenciado que os níveis séricos de 25(OH)D apresentam associação inversa com adiposidade corporal e resistência à insulina (RI) na população em geral e na DRC. O excesso de gordura corporal e o risco de Doença Cardiovascular (DVC) vêm sendo estudados em pacientes com DRC e dentre as complicações metabólicas associadas à adiposidade corporal elevada observa-se valores aumentados de HOMA-IR (Homeostasis Model Assessment of Insulin Resistance) um marcador para RI. Estudos avaliando o perfil da 25(OH)D na DRC na fase não dialítica, especialmente relacionados com a adiposidade corporal e RI são escassos. O presente estudo tem como objetivo avaliar a relação entre os níveis séricos de 25(OH)D, RI, e adiposidade corporal em pacientes com DRC na fase não dialítica. Trata-se de um estudo transversal observacional, incluindo pacientes adultos, clinicamente estáveis e com filtração glomerular estimada (FGe) ≤ 60 ml/min., em acompanhamento regular no Núcleo Interdisciplinar de Tratamento da DRC. Os participantes foram submetidos à avaliação do estado nutricional por antropometria (peso, altura, índice de massa corporal (IMC), circunferências e dobras cutâneas) e absorciometria de duplo feixe de raios X (DXA); foram avaliados no sangue: creatinina, uréia, glicose, albumina, colesterol total e frações e triglicérides, além de leptina, insulina e 25(OH)D. Níveis séricos < 20ng/dL de 25(OH)D foram considerados como deficiência. As análises estatísticas foram realizadas utilizando-se o software STATA versão 10.0, StataCorp, College Satation, TX, USA. Foram avaliados 244 pacientes (homens n=135; 55,3%) com média de idade de 66,3 13,4 anos e de FGe= 29,4 12,7 ml/min. O IMC médio foi de 26,1 kg/m (23,0-30,1) com elevada prevalência de sobrepeso/obesidade (58%). A adiposidade corporal total foi elevada em homens (gordura total-DXA= 30,2 7,6%) e mulheres (gordura total-DXA= 39,9 6,6%). O valor mediano de 25(OH) D foi de 28,55 ng/dL (35,30-50,70) e de HOMA-IR foi 1,6 (1,0-2,7). Os pacientes com deficiência de 25(OH)D (n= 51; 20,5%) apresentaram maior adiposidade corporal total (DXA% e BAI %) e central (DXA%) e valores mais elevados de leptina. A 25(OH)D apresentou correlação significante com adiposidade corporal total e central e com a leptina, mas não se associou com valores de HOMA-IR. Estes resultados permitem concluir que nos pacientes DRC fase não dialítica a deficiência de 25(OH)D e a elevada adiposidade corporal são frequentes. Estas duas condições estão fortemente associadas independente da RI; a alta adiposidade corporal total e central estão positivamente relacionadas com RI; 25(OH)H e RI não estão associados nessa população com sobrepeso/obesidade.

Reactions of fishes to two underwater survey tools, a manned submersible and a remotely operated vehicle

We examined the reactions of fishes to a manned submersible and a remotely operated vehicle (ROV) during surveys conducted in habitats of rock and mud at depths of 30–408 m off central California in 2007. We observed 26 taxa for 10,550 fishes observed from the submersible and for 16,158 fishes observed from the ROV. A reaction was defined as a distinct movement of a fish that, for a benthic or hovering individual, was greater than one body length away from its initial position or, for a swimming individual, was a change of course or speed. Of the observed fishes, 57% reacted to the ROV and 11% reacted to the submersible. Aggregating species and those species initially observed off the seafloor reacted most often to both vehicles. Fishes reacted more often to each vehicle when they were >1 m above the seafloor (22% of all fishes >1 m above the seafloor reacted to the submersible and 73% to the ROV) than when they were in contact with the seafloor (2% of all reactions to the submersible and 18% to the ROV). Fishes reacted by swimming away from both vehicles rather than toward them. Consideration of these reactions can inform survey designs and selection of survey tools and can, thereby, increase the reliability of fish assemblage metrics (e.g., abundance, density, and biomass) and assessments of fish and habitat associations.

Hydrodynamic entrapment of bacteria swimming near a solid surface.

The near-surface motility of bacteria is important in the initial formation of biofilms and in many biomedical applications. The swimming motion of Escherichia coli near a solid surface is investigated both numerically and experimentally. A boundary element method is used to predict the hydrodynamic entrapment of E. coli bacteria, their trajectories, and the minimum separation of the cell from the surface. The numerical results show the existence of a stable swimming distance from the boundary that depends only on the shape of the cell body and the flagellum. The experimental validation of the numerical approach allows one to use the numerical method as a predictive tool to estimate with reasonable accuracy the near-wall motility of swimming bacteria of known geometry. The analysis of the numerical database demonstrated the existence of a correlation between the radius of curvature of the near-wall circular trajectory and the separation gap. Such correlation allows an indirect estimation of either of the two quantities by a direct measure of the other without prior knowledge of the cell geometry. This result may prove extremely important in those biomedical and technical applications in which the near-wall behavior of bacteria is of fundamental importance.

Brevetoxin in two planktivorous fishes after exposure to Karenia brevis: implications for food-web transfer to bottlenose dolphins

Brevetoxin uptake was analyzed in 2 common planktivorous fish that are likely foodweb vectors for dolphin mortality events associated with brevetoxin-producing red tides. Fish were exposed to brevetoxin-producing Karenia brevis for 10 h under conditions previously reported to produce optimal uptake of toxin in blood after oral exposure. Striped mullet Mugil cephalus were exposed to a low dose of brevetoxin, and uptake and depuration by specific organs were evaluated over a 2 mo period. Atlantic menhaden Brevoortia tyrannus specimens were used to characterize a higher brevetoxin dose uptake into whole body components and evaluate depuration over 1 mo. We found a high uptake of toxin by menhaden, with a body to water ratio of 57 after a 10 h exposure and a slow elimination with a half life (t1/2) of 24 d. Elimination occurred rapidly from the intestine (t1/2 < 1 wk) and muscle (t1/2 ≈ 1 wk) compartments and redistributed to liver which continued to accumulate body stores of toxin for 4 wk. The accumulation and elimination characteristics of the vectoring capacity of these 2 fish species are interpreted in relation to data from the Florida Panhandle dolphin mortality event of 2004. We show that due to slow elimination rate of brevetoxin in planktivorous fish, brevetoxin-related dolphin mortality events may occur without evidence of a concurrent harmful algal bloom event.

Some aspects of reproductive biology of two sciaenids, Otolithes cuvieri Trewavas and Johnius elongatus Mohan: maturation, spawning, sex ratio and fecundity

The gonads of Otolithes cuvieri and Johnius elongatus are described in seven maturity stages. O. Cuvieri spawns once a year from April to September as evidence by ova diameter frequency distribution and GSI values. 50% maturity is attained at 210mm TL in males and 200mm TL in females. Fecundity ranged from 2387 to 104379 with a mean value of 33502. Log-Log relationship between fecundity and total lenght, body weight and ovary weight were determined. An overall sex ratio of 1.54:1.00 was unequal in favour of males. Johnius elongatus spawns twice a year from January-February to Aprile-May and from August to October as evidence by ova diameter frequency distribution and GSI values. 50% maturity is attained at 140-143mm TL in both sexes. Fecundity ranged from 4238 to 167669 with a mean value of 42818. Log-Log relationship between fecundity and total lenght, body weign and ovary weight were determined. An overall sex ratio of 1.00:1.20 was unequal in favour of females.

A study on the lipid distribution pattern in muscles of two teleosts, Arius dussumieri and Ophiocephalus striatus

The muscles of the various regions and zones of the body of the two teleosts, A. dussumieri and O. striatus have been analyzed for lipid contents. There is a significant dorsum-ventral gradient in lipid concentration exhibited by both the fishes with higher lipid values in the ventral aspect of the body, especially the belly flaps. As regards the vertical series, both the fishes exhibit comparatively higher lipid contents at the dorsal aspects of the caudal region and at anterior portion of the dorsal fin area with lower lipid values at cephalic and middle portions of the body. The red muscle of Arius exhibits higher lipid content than the white muscle.

Correlation between density of states distributions and the fermi level position in interfacial regions of amorphous silicon thin film transistors

This paper investigates the variation of the integrated density of states with conduction activation energy in hydrogenated amorphous silicon thin film transistors. Results are given for two different gate insulator layers, PECVD silicon oxide and thermally grown silicon dioxide. The different gate insulators produce transistors with very different initial transfer characteristics, but the variation of integrated density of states with conduction activation energy is shown to be similar.

A comparative study of the morphometrics and the proximate composition of two edible molluscs Crassostrea cuculata (Born) and Perna perna (L.)

The morphometrics and the bio-chemical characteristics of Crassostrea cuculata and Perna perna were studied. In the oyster, the length was found to be positively correlated with breadth and total body weight. There was also a positive relationship between the total body weight and the total muscle weight. In the mytilid the total body weight was positively correlated with length and total muscle weight. The muscle weight was also found to be highly correlated with the length. In both animals the relationship between the length and the total body weight was curvilinear. In the oyster, the protein content was found to increase with the total body weight and the total muscle weight. In the mytilid the protein content increased not only with the total body weight but also with the length. In both animals, the relative content of protein is higher than that of carbohydrates, lipids and ash.

Early developmental stages of two Secutor species (Family: Leiognathidae) collected from the Bak-khali river estuary of the Bay of Bengal, Bangladesh

Early developmental stages of two Secutor species, Secutor insidiator (Bloch) (11.9-36.0 mm standard length, SL) and Secutor ruconius (Hamilton-Buchanan) (14.0-33.0 mm SL) collected by ichthyoplankton net from the Bak-khali river estuary of the Bay of Bengal, Cox's Bazar, Bangladesh are described and illustrated. All of the fins with supporting spines and rays were present in the smallest collected sizes of both species. With growth of the specimens, significant changes in melanophore patterns were found. S. insidiator is similar to S. ruconius in having upward protracting mouth parts and body colouration, but can be distinguished easily by its more elongate body shape (body depth 38-47% of SL compared with 46-52% of SL in Secutor ruconius). Both the species occurred round the year from August 1998 to July 1999. The surface water temperature and salinity during the study period varied from 22.0-32) C and 10-37 ppt respectively.

Reproductive biology, artificial propagation and larval rearing of two freshwater eels, Monopterus cuchia and Mastacembelus armatus

Studies on reproductive biology and artificial propagation including larval rearing of freshwater mud eel, Monopterus cuchia and spiny eel, Mastacembelus armatus were attempted. The gonadosomatic index (GSI) of mud eel ranged from 0.41 (August) to 5.52 (June) in males and 0.53 (August) to 7.61 (June) in females. In both cases the GSI showed a peak in June. Fecundity ranged from 228 (TL - 396 mm; W - 78g) to 5510 (TL - 865 mm; W - 630 g). In case of spiny eel, the GSI varied from 0.65 (August) to 8.30 (July) in males and 0.70 (August) to 10.46 (July) in females. GSI showed single peak in July. Fecundity ranged from 570 (TL - 240 mm; W - 30 g) to 10870 (TL - 601; W - 350g). Histology of the testes and ovaries of the eels were carried out to investigate the gonadal development stages during the reproductive months (August to November 2003). In case of male M. cuchia, the secondary primordial germ cells, primary spermatogonium, some spermatogonia A and clone of spermatogonium B in testis were observed in September. In October-males different sized lobules having spermatogonia, spermatocytes and spermatids were observed. In the ovary of M. cuchia, polygonal shaped oocytes were seen during September. The oogonia were reduced with dense and irregular shaped during October. Numerous pycnotic cells were visible during November. In male M. armatus numerous broken lobule walls were found in testes during September. In October, abundant primary germ cells, pycnotic nests of degenerating cells, spermatogonia and spermatids were observed. In females, ovaries had distinct yolk vesicles stage and yolk granules stages in August. In September, the follicular cells of the oogonia were ruptured, shrunk forming irregular shaped in October. Oogonia were also shrunk with thin, irregular shaped structure but broken parts of the ruptured follicular cells were scattered in case of M. armatus. Experimental attempts on artificial propagation indicated that both freshwater eels were difficult to breed using inducing agents like pituitary glands (PG) of 10, 20, 50, 100 and 150 mg per kg of body weight. Same doses were used for both sexes with equal sex-ratio. In both cases, brood fish died at higher doses of injection given at 100 and 150 mg PG/kg bodyweight. However, M. cuchia breed naturally in cisterns when provided with water hyacinths and tunnel in muddy bottom. M. cuchia fed with chopped cooked fish attained a mean weight of 18.75 ± 2.3 g and cent percent survival. While in case of M. armatus best growth by weight (12.0 ± 2.48 g) and cent percent survival were achieved using chopped raw fish. Car tyre was observed as best shelter for attaining the mean weight gain 22.53 ± 2.24 g and cent percent survival of M. cuchia. While PVC pipe was found to be the best shelter for M. armatus, where it attained the mean weight of 12.73 ± 1.88 g and cent percent survival.

Population dynamics of two jewfishes Jhonius argentatus and Johnieops vogleri) in the coastal waters of Bay of Bengal, Bangladesh

Population parameters of Jhonius argentatus and Johnieops vogleri in coastal waters of Bay of Bengal, Bangladesh were estimated by using FiSAT programme. The von Bertalanffy growth parameters, extreme length (cm) and growth constant K (year ·1) were found to be 46.50 and 0.59 for J. argentatus, and 33.50 and 0.85 for J.vogleri The Loc(cm) and Z/K estimates provided by Wetherall plot were 46.694 and 1.791 for J. argentatus, and 31.25 and 2.623 for J. vogleri. The annual rate of natural (M) and fishing mortality (F) were estimated as 1.12 and 0.78 for J. argentatus, and 1.56 and 1.28 for J. vogleri. Rate of exploitation (E) was estimated as 0.41 for J. argentatus and 0.45 for J. vogleri. About 80.04% of J. argentatus were found to be recruited during peak pulses (April-May) and 19.96% during lean pulses (October-November) and 85.75% J. vogleri during peak pulses (May-July) and 14.25% during lean pulses (September-October). The growth performance index(') was 3.11 for J. argentatus and 1.93 for J. vogleri. The total length and body weight relationship was found to be W = 0.0403 TL25723 for J. argentatus and W = 0.0907 TV3482 for J. vogleri.

Microbial carbonate precipitation: Correlation of S-wave velocity with calcite precipitation

The use of microbial induced precipitation as a soil improvement technique has been growing in geotechnical domains where ureolytic bacteria that raise the pH of the system and induce calcium carbonate (CaCO3) precipitation are used. For many applications, it is useful to assess the degree of CaCO 3 precipitation by non-destructive testing. This study investigates the feasibility of S-wave velocity measurements to evaluate the amount of calcite precipitation by laboratory testing. Two sets of cemented specimen were tested. The first were samples terminated at different stages of cementation. The second were samples that went through different chemical treatments. These variations were made to find out if these factors would affect the S-wave velocity- cementation relationship. If chemical reaction efficiency was assumed to be constant throughout each test, the relationship between S-wave velocity (Vs) and the amount of CaCO3 precipitation was found to be approximately linear. This correlation between S-wave velocity and calcium carbonate precipitation validates its use as an indicator of the amount of calcite precipitation © 2011 ASCE.

Seasonal fluctuation in primary production in relation to the physicochemical parameters of two weed-infested ponds of Kalyani, West Bengal

Kalyani lake (P sub(1)), a weed infested recreational water body and a weed chocked derelict water body (P sub(2)) in the heart of Kalyani city of West Bengal were studied for a period of one year for their primary productivity and other physicochemical parameters. Very low primary productivity (GPP=360-1237mg C m super(-2) d super(-1); NPP=157-787 mg C m super(-2) d super(-1)) was recorded in P sub(2) in spite of having a high concentration of nutrients (PO sub(4)–P=0.052-0.260mg l super(- 1); NO sub(3)-N=0.110-0.412mg l super(-1)). On the other hand, moderate primary productivity (GPP=1687-3195mg C m super(-2) d super(-1); NPP=900-2700mg C m super(-2) d super(-1)) was found in P sub(1) with comparatively low range of nutrients (P0 sub(4)-P =0.010-0.058mg l super(-1); NO sub(3)-N=0.032-0.118mg l super(-1)). After studying the other physicochemical parameters (temperature, transparency, dissolved oxygen, free carbon dioxide, pH, alkalinity and macrophytic biomass), it was found that the overall hydro-biological conditions of the weed-chocked derelict water body (P sub(2)) is not congenial for biological production as compared to Kalyani Lake (P sub(1)). Kalyani Lake may be used for fish culture with proper management practices.

Estimation of RNA/DNA ratio in two north-Indian natural populations of mahseer (Tor tor) and its relationship with growth and hydrobiology

Samples of Tor tor were collected from Bari Reservoir of Udaipur and Narmada River at Hoshangabad (India), in the months of July and November 2005, respectively. Twenty-five samples were collected from each location. Bari Reservoir samples ranged from 17.0 to 24.5 cm in total length and from 75 to 155 g in weight, while Narmada samples ranged from 20.0 to 42.0 cm in length and 90 to 425 g in weight. The nucleic acid content in body muscle of Tor tor and the RNA/DNA ratio were estimated. The age of fishes was estimated by the scale study method and specimens were classified into four age groups. RNA/DNA ratio showed significant linear increase with increase in weight and age till the age of three years after which, the growth rate reduced. The 1-2 year group was the only one common between the two water bodies and a comparison of RNA/DNA ratios showed higher growth rate in Bari Reservoir. The gross primary productivity was also higher in Bari Reservoir being 551 mg cmˉ³ dˉ¹ compared to 404 mg cmˉ³ dˉ¹ observed for Narmada River. The condition factor (K) was found to be higher (1.21) in the fish from the Bari Reservoir compared to those of Narmada River (1.14). The growth rate was higher in females compared to males in >100 g specimens.

Ecology and production in two sandy beaches of Goa. Part (A) Distribution of bacteria in Colva and Siridaon in relation to certain environmental parameters

Qualitative and quantitative studies on the bacterial flora of two beaches viz., Colva and Siridaon at high tide, mid-tide and low tide levels during the pre-monsoon period were made. Estimations of important nutrients, like inorganic phosphates and nitrates and organic carbon have been made and correlation with the bacterial counts is being attempted. Definite correlation between bacterial population and organic carbon is seen however, no correlation is observed between bacterial counts and other physicochemical parameters.