A study of the anti-plasmodium activity of angiotensin II analogs

Controlling the dissemination of malaria requires the development of new drugs against its etiological agent, a protozoan of the Plasmodium genus. Angiotensin II and its analog peptides exhibit activity against the development of immature and mature sporozoites of Plasmodium gallinaceum. In this study, we report the synthesis and characterization of angiotensin II linear and cyclic analogs with anti-plasmodium activity. The peptides were synthesized by a conventional solid-phase method on Merrifield's resin using the t-Boc strategy, purified by RP-HPLC and characterized by liquid chromatography/ESI (+) MS (LC-ESI(+)/MS), amino acid analysis, and capillary electrophoresis. Anti-plasmodium activity was measured in vitro by fluorescence microscopy using propidium iodine uptake as an indicator of cellular damage. The activities of the linear and cyclic peptides are not significantly different (p < 0.05). Kinetics studies indicate that the effects of these peptides on plasmodium viability overtime exhibit a sigmoidal profile and that the system stabilizes after a period of 1 h for all peptides examined. The results were rationalized by partial least-square analysis, assessing the position-wise contribution of each amino acid. The highest contribution of polar amino acids and a Lys residue proximal to the C-terminus, as well as that of hydrophobic amino acids in the N-terminus, suggests that the mechanism underlying the anti-malarial activity of these peptides is attributed to its amphiphilic character.

The whole works of John Flavel, late minister of the gospel at Dartmouth, Devon.

I. The life of John Flavel. The fountain of life.--II. The method of in the gospel-redemption. Pneumatologia; a treatise of the soul of man.--III. Pneumatologia (cont.) A practical treatise of fear. The righteous man's refuge. A blow at the root; or, The causes and cure of mental errors. Vindiciarum vindex. The second appendix; of Antinomianism. A sermon on gospel-unity.--England's duty. Mount Pisgah. Divine conduct; or, The mystery of Providence. Wonderful sea-deliverances. Antipharmacum saluberrimum. Tidings from Rome; or, England's alarm.-- V. Husbandry spiritualized; or, The heavenly use of earthly things. Navigation spiritualized; or, A new compass for seamen. [Miscellaneous tracts and sermons]--VI. Preparations for sufferings. The balm of the covenant. An exposition of the Assembly's Shorter catechism. A reply to Mr. Philip Cary's Solemn call. Sacramental meditations. [Miscellaneous tracts and sermons].

History of the brewing industry and brewing science in America, prepared as part of a memorial to the pioneers of American brewing science, Dr. John E. Siebel and Anton Schwarz,

Acknowledgment, [1] p. at end.

The family expositor: or, A paraphrase and version of the New Testament; with critical notes, and a practical improvement of each section ...

Edited by Job Orton.

The works of John Otway, consisting of his plays, poems, and letters. With a sketch of his life, enlarged from that written by Dr. Johnson.

I. Alcibiades. Don Carlos, prince of Spain. Titus and Berenice. The cheats of Scapin. Friendship in fashion. The soldier's fortune.--II. The atheist; or, The second part of The soldier's fortune. The orphan. The history and fall of Caius Marius. Venice preserved. Poems and letters.

Paul the apostle, pioneer missionary to the heathen world,

Mode of access: Internet.

Lock's Essay for the understanding of St. Paul's epistles, and Le Clerc on inspiration.

An essay for the understanding St. Paul's epistles, &c. -- Le Clerc On ispiration.

The works of the Right Rev. John Sage, a bishop of the church in Scotland; with memoir and notes ...

Vol. 2 in 2 parts, pt. [2] of which is paged continously with v. 3.

The life of John Locke, with extracts from his correspondence, journals, and common-place books.

Goldsmiths'-Kress no. 26095.

Harrison's Description of England in Shakspere's youth. Being the second and third books of his Description of Britaine and England.

A fourth part (The supplement, # 2) planned by the society, was published 1908 in The Shakespeare library.

Paul, the herald of the cross,

Mode of access: Internet.

Brain and mind in the 'long' eighteenth century

How should the 'long' eighteenth century be defined? January 1, 1700 and December 31, 1799 are quite arbitrary dates. Why should they be chosen to segment our history rather than more significant periods of time, periods which have a coherent content, or are marked, perhaps, by the working out of a theme? Students of English literature sometimes take the long eighteenth century to extend from John Milton (Paradise Lost, 1667) to the passing of the first generation of Romantics (Keats (d. 1821), Shelley (d. 1822), Byron (d. 1824), Coleridge (d. 1834)). Students of British political history often take it to start with the accession of Charles II (the Restoration) in 1660 or, alternatively, the so-called Glorious Revolution of 1688 and to end with the great Reform Act of 1832. Others might choose different book ends. In the history of science and philosophy the terminus a quo is sometimes taken as the publication of Descartes' scientific philosophy or, in more Anglophone zones, the 1687 publication of Newton's Principia with its vision of a 'clockwork universe'. 'Nature and Nature's laws' as Alexander Pope enthused, 'lay hid in Night: God said, Let Newton be! and all was light!'.

EPIPOX:immunoinformatic characterization of the shared T-cell epitome between variola virus and related pathogenic orthopoxviruses

Concerns that variola viruses might be used as bioweapons have renewed the interest in developing new and safer smallpox vaccines. Variola virus genomes are now widely available, allowing computational characterization of the entire T-cell epitome and the use of such information to develop safe and yet effective vaccines. To this end, we identified 124 proteins shared between various species of pathogenic orthopoxviruses including variola minor and major, monkeypox, cowpox, and vaccinia viruses, and we targeted them for T-cell epitope prediction. We recognized 8,106, and 8,483 unique class I and class II MHC-restricted T-cell epitopes that are shared by all mentioned orthopoxviruses. Subsequently, we developed an immunological resource, EPIPOX, upon the predicted T-cell epitome. EPIPOX is freely available online and it has been designed to facilitate reverse vaccinology. Thus, EPIPOX includes key epitope-focused protein annotations: time point expression, presence of leader and transmembrane signals, and known location on outer membrane structures of the infective viruses. These features can be used to select specific T-cell epitopes suitable for experimental validation restricted by single MHC alleles, as combinations thereof, or by MHC supertypes.