Método de quantificação da capacidade antioxidante total sérica: padronização e validação para espécie ovina

Pós-graduação em Ciência Animal - FMVA

Avaliação da maturidade pulmonar de cordeiros nascidos a termo e prematuros pela análise citológica, Test de Clements e contagem de corpos lamelares do líquido amniótico

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Isolamento de Streptococcus agalactiae em diferentes órgãos de tilápias do nilo (Oreochromis niloticus) criadas em tanques-rede

Streptococcosis is one of the major causes of mortality in tilapia's creation in Brazil, inducing great economic losses. As soon, the study objectived to determinate the frequency of isolation and identification the Streptococcus agalactiae in organs different of Oreochromis niloticus naturally infected, derived from eight fish farms in the northern region of the state of Parana, that presented clinical signs characteristics of streptococcal disease. However, blood samples and fragments (kidney, liver, spleen, heart and brain) were collected. These all samples were plated on solid medium of brain and heart infusion (BHI) added 5% ovine blood and incubated at 29 degrees C for 7 days in aerophilic conditions. Behind, the bacterial growth and from the macro and microscopic features, colonies compatibles with Streptococcus sp. gender, were selected. The species were identified by PCR reaction and confirmed by sequencing of 16S rDNA gene. The results exhibited that in tilapia of Nile infected with S. agalactiae the isolation is more common in brain, kidney and liver in descending order.

Effects of the morphine-lidocaine-ketamine combination on cardiopulmonary function and isoflurane sparing in sheep

The aims of this study were to evaluate the isoflurane sparing and clinical effects of a constant rate infusion of morphine - lidocaine - ketamine (MLK) in healthy sheep undergoing experimental gastrointestinal surgery. Twelve adult female sheep (Texel breed) were used, weighing 36.5 +/- 8.1 kg. The sheep were anesthetized for the implantation of duodenal cannulas. The sheep were premedicated with 0.3 mg kg(-1) intramuscular (IM) morphine and 20 mu g kg(-1) intravenous (IV) detomidine. After premedication, anesthesia was induced using 5 mg kg(-1) ketamine and 0.5 mg kg(-1) diazepam IV and maintained using isoflurane in 100% oxygen. After the induction of anesthesia, the animals were allocated into two groups (each n=6); the GMLK (MLK group - 10 mg morphine, 150 mg lidocaine, 30 mg de ketamine were added in 500 mL saline) received a 10 mL kg(-1)h(-1) MLK infusion during the maintenance of anesthesia, and GCON (control group) received 10 mL kg(-1)h(-1) of 0.9% sodium chloride. The animals were mechanically ventilated. Cardiopulmonary variables and end-tidal isoflurane concentration (FE'Iso) were measured at baseline (immediately before the surgery) and 15, 30 and 45 minutes after initiation of surgery. In GMLK, there was a decrease in the FE 'Iso at 15, 30 and 45 minutes, a reduction of up to 75.6% during the surgery. The HR was lower in GMLK compared with GCON at 30 minutes, and the MAP was at during baseline in GCON compared with GMLK. The standing time was less in GMLK than in GCON. The use of intravenous MLK was demonstrated to offer great efficiency as part of a balanced anesthesia protocol in sheep, with a 75.6% reduction in the need for isoflurane, providing stability of the cardiovascular parameters and blood gases with a shortened recovery period.

Avaliação da vitalidade de cordeiros nascidos de partos eutócicos e cesarianas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Improvement to the physical quality and imbibition pattern in seeds of candeia (Eremanthus incanus (Less.) Less.)

The objective of this work was to develop a methodology for seed processing and X-ray analysis and to study imbibition pattern in seeds of candeia (Eremanthus incanus). Seeds were gathered in 2001 and 2002 in Morro do Pilar and Lavras respectively, processed and stored in a cold chamber (5ºC/60%RH) until the start of experiments. In order to identify unfilled seeds, a protocol was developed for X-ray use combining radiation energy (Kv) and exposure time (seconds). For elimination of unfilled seeds, an experiment was conducted using a South Dakota seed blower whereby different opening settings and ventilation times were combined. Original seed lots and categorized lots following radiograph viewing were tested by germination tests, with seeds being cleansed in sodium hypochlorite and scattered over blotting paper in 'gerboxes' and then taken to germinators with alternating temperatures of 20º-30ºC and 10 hours of light, for up to 15 days. Imbibition curves were determined under the conditions 30ºC, 20º-30ºC/10 hours of light and 30º-20ºC/10 hours of light. The X-ray protocol that best allowed viewing of internal seed structures was 30Kv for 45 seconds. Seed separation using the blower setting at a 3.0 opening for 30 seconds raised the number of embryonic seeds to values exceeding 99%, also raising the germination rates as a consequence. The imbibition pattern is three-phased and the germination process is completed in 72 hours. Under alternate temperatures, primary root growth is favored.

Efeito de diferentes frações inspiradas de oxigênio sobre a ação anti-hipertensiva pulmonar do sildenafil

Pós-graduação em Biotecnologia Animal - FMVZ

Efeitos da adrenomodulina no tratamento da hipertensão pulmonar induzida por microesferas em ovinos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Supressão temporária do crescimento folicular ovariano em ovelha pela administraçao continuada de deslorelina na forma injetável ou implante de liberação controlada

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Desenvolvimento inicial e densidade de estocagem de larvas de Aracu-riscado, Leporinus agassizii (Characiformes: Anostomidae)

Pós-graduação em Aquicultura - FCAV

Perfil de Aglutininas Anti-leptospira e anti-brucella e condições sanitárias de ovinos da região noroeste do Estado de São Paulo, Brasil

The sheep industry has become increasingly prominent in agribusiness, transforming the stage production of Brazil, and thus contributes more to the socio-economic development of the country. The work aimed to verify the occurrence of brucellosis and leptospirosis in sheep from northwestern São Paulo state. In addition to determining the prevalence of major Leptospira in the region and to trace the diagnosis of sheep breeding in this part of the country. All the 1222 sheep serum samples from 49 properties did not react serologically to evidentiary testing for brucellosis, compared to antigens of B. abortus and B. ovis used in the testing of 2-ME and IDGA, respectively. The Microscopic Agglutination Test (MAT) test revealed that 19.14% (232/1212) of samples were positive for one or more serovars, with titles ranging from 100 to 800. The most frequent serovar was hebdomadis in the region, with Sentot and Sherman (18.10%, 11.64% and 8.62%, respectively). By profiling the system of sheep farming in the region, we found that most herds are composed of more than one race being the main purpose is for the court. According to the scheme adopted immunoprophylactics there is a homogeneous set schedule. It adopts the use of anti-helminth, non-prescription veterinary antibiotic. There is also the presence of diarrhea and abortions and the lack of criteria for los ovinos on management, making it the need for emergency development of programs for disease control, schema immunoprophylactics adequate sanitation and hygienic measures in sheep breeding.

Lipid structural information from a single equine embryo by MALDI-MS

O objetivo deste trabalho foi relatar o potencial da técnica de MALDI-MS para caracterizar espécies de lipídios presentes em um único embrião equino e estudar algumas estruturas lipídicas detectadas por dissociação induzida por colisão (CID). No espectro de modo íon positivo, pudemos observar espécies, principalmente, protonadas e sodiadas de esfingomielinas (SM), fosfatidileolinas (PC) e triacilgliceróis (TAG). No modo negativo, observamos fosfatidiletanolaminas (PE) e fosfatidilinositos (PI). Espectros de íons de lípidos com maior intensidade foram utilizados para demonstrar o potencial da informação estrutural por MALDI-MS/MS. O espectro no modo positivo de m/z (massa sobre carga) 760,6 (atribuída como PC34:1) apresentou características de fragmentos PC de m/z 184,1 (denominada cabeça polar de colina), além de perda neutral (NL) de m/z 183 (fosforilcolina). Para o íon de m/z 766,6 (atribuída como PE38:5), observou-se a NL de 140, característica do PE. Para o íon de m/z 808,7 (38,5 atribuído como PC), além do fragmento m/z 184,1 na NL de 183, foi possível observar a perda de trimetilamina (íon de m/z 749,6) e o ciclofosfano (íon de m/z 147,0). Finalmente, para o modo de íon negativo, foram isolados e fragmentados o íon de m/z 863,6 que foi atribuído como PI36:1, devido à presença de m/z 153 (fosfato de glicerol – H2O-H ), 223 (inositol fosfo - 2H2O-H) , 241 (fosfoinositol – H2O-H), 281 (ácido oleico) e 581,3 (lisofosfoinositol – H2O+H). Concluímos que a MALDI - MS permite a detecção de uma ampla gama de espécies de PC, SM, PE, PI e TAG lipídicas, bem como a caracterização rápida e confiante de estruturas lipídicas a partir de um único embrião equino.

Fisiologia da termorregulação e estresse térmico em ovinos lanados

The introduction of wool sheep in warm temperature regions of Brazil has also raised concerns regarding the warm stress and its consequent decrease in animal production. The understanding of the physiology of thermal balance in sheep, as well as the consequences of this phenomenon, should be studied in order to improve animal thermal comfort in association with the production and reproduction indices of these animals. The majority of available research findings related to thermal balance in sheep is derived from countries with temperate climates and therefore might be different from those performed in our climate conditions. This essay presents a literature review that intends to discuss this theme.

Qualidade das silagens de sorgo e de planta inteira de soja na terminação de cordeiros em confinamento

Pós-graduação em Zootecnia - FMVZ

Milho, amido ou caroço de algodão associados à glicerina bruta em dietas para ovinos

Pós-graduação em Zootecnia - FCAV