Efeito das fibras alimentares como substitutos de gordura em hambúrguer de carne bovina e paio

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Purificação parcial e caracterização bioquímica de uma isoforma de β-glicosidase do fungo termofílico Myceliophthora thermophila M.7.7

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Ciclagem de carbono em área sob semeadura direta e aplicação de lodo de esgoto

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Produção e caracterização de amilases bacterianas: α-amilase e ciclodextrina glucanotransferase (CGTase)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Digestibilidade do amido e disponibilidade de ca e p em alimentos energéticos extrusados para a tilápia do nilo (Oreochromis niloticus)

Apparent digestibility coefficients (ADC) of starch and calcium (Ca) and phosphorus (P) apparent availability were evaluated in five cereal grain products and byproducts (corn, wheat meal, rice grain, rice bran and sorghum) for Nile tilapia. Chromic oxide was used as an external digestibility marker. The highest Ca and P apparent availability, respectively, were obtained for rice grain (43.03 and 64.79%), sorghum (39.89 and 58.09%) and corn (22.18 and 19.48%), while rice bran (-31.49 and 3.25%) and wheat meal (5.80 and 1.18%) showed the lowest values. Starch digestibility varied between 99.45 and 95.59% among the evaluated ingredients. This high ADC of starch observed in this study confirms that Nile tilapia is able to efficiently digest and utilize complex carbohydrates.

Desempenho agronômico e diagnose foliar da aveia preta em função da calagem em plantio direto

The soil acidity in no tillage system could be resolved by lime superficial application, improvement to crop mineral nutrition and yield. The experiment carried out on a Rhodic Kandiudalf in Botucatu, São Paulo State, Brazil, in dry conditions, aiming to evaluate the agronomic development and leaf diagnosis of black oat plants, under superficial liming in no tillage system. A randomized complete block design was used, with four replications, the treatments were superficial application of different dolomítico lime rates (R0 = zero – without lime; R1 = 1,8 t ha-1 – lime to increase the base saturation at 50%; R2 = 3,6 t ha-1 – lime to increase the base saturation at 70% and R3 = 5,4 t ha-1 – lime to increase the base saturation at 90%), in October 2002. The black oat Common cultivar was seed in April 2004, in to second year, after the crop rotation of millet (spring) – common bean (summer) – black oat (autumn-winter). The results showed that: the black oat had yield increase by superficial liming, mainly to most dry matter and grain per panicule. Beyond, the superficial liming application did not prejudice leaf diagnosis of culture.

Desempenho de discos de corte de semeadora-adubadora em diferentes quantidades de cobertura vegetal

The quality and efficiency in straw cutting are determinants in the sowing process, according to the occurrence of "tamp" and stops, the magnitude of the straw removal or even the known effects of the seed "envelopment" where the cutting mechanism can not cut the remaining straw. Thus, the objective of this study was to evaluate the performance of different mechanisms for straw cutting of a seeder individualized in different amounts of crop coverage artificially added in a clay soil. The experiment was conducted at Unesp – Sao Paulo State University in the Experimental Farm Lageado, belonging to the College of Agricultural Sciences - Unesp, Botucatu. The experimental design was split plot with four replications, with main plots consisting of five quantities of black oat straw, in area with no-tillage system and subplots of three opening furrows mechanisms, which are: plane disk (PD), corrugated disk (CD) and wavy disk (WD). The wavy disk provided greater rolling resistance, reducing the speed of the set (tractor and equipment). The plane cutting disk showed the highest values of mobilized soil area, deeper penetration into the soil, requiring lower values of vertical force and higher values of lateral force. Regarding the type of waste and doses used, they offered no resistance to cutting and there was no change in the horizontal force on straw.

Influência da temperatura no crescimento micelial de linhagens de Lentinula edodes

The objective of the present work was to evaluate the in vitro mycelial growth of ten L. edodes strains (LED 12, LED 20, LED 25, LED 27, LED 33, LED 35, LED 51, LED 55, LED 58 and LED 75) submitted to the temperatures of 15, 20 and 25 ºC. An agar medium prepared with eucalyptus wood extract and soy bran was used and radial measurement of the mycelial growth of L. edodes strains was performed. The experimental design was totally randomized, in a 10 x 3 factorial scheme. Each treatment corresponded to a Petri plate and consisted of 5 repetitions. It was verified that L. edodes growth is influenced by the incubation temperature, that is the temperature of 25 ºC was the most favorable for the mycelial growth of all L. edodes strains, especially for LE 75, LE 55, LE 33 and LE 12 strains, which obtained the highest mycelial growth averages at 25 ºC at the end of the cultivation cycle.

Uso de resíduos madeireiros da Amazônia brasileira no cultivo in vitro de Lentinus strigosus

Lentinus strigosus (Schwein.) Fr. is an exploitable edible mushroom occurring in the Brazilian Amazon, being part of a huge diversity of edible mushrooms which are little grown. The use of regional waste is recommended to reduce production costs of any kind of edible mushroom. Thus, the mycelial growth of L. strigosus in culture media based on regional wood waste extract by using substrates based on Protium puncticulatum, Cariniana micrantha and Caryocar glabum sawdust, supplemented with 20% of wheat bran (Triticum aestivum), corn bran (Zea sp.) or rice bran (Oryza sp.) was observed. Eucalyptus (Eucaliptus sp.) sawdust was used for comparison with the other wood wastes because it is commonly used in the cultivation of edible fungi. The experimental design employed was totally randomized, in 4 x 3 factorial scheme (sawdust x bran), adding up 12 treatments with 5 repetitions, being that each repetition corresponded to a Petri dish, totalizing 60 dishes, incubated at 35 ºC. The diameter of the colony was daily evaluated until the fungus reached the borders of the Petri dish in one of the treatments. After that period, the media based on P. puncticulatum sawdust obtained thebest results of mycelial growth, showing potential to be used as an alternative residuein a future production of L. strigosus in the state of Amazonas.

Efeito de fungos contaminantes na produtividade de dois isolados de Agaricus blazei em duas formulações de composto

The effect of the contaminant fungi Trichoderma sp. and Chaetomium olivacearum on the cultivation of the ABL 99/30 and ABL 04/49 isolates of A. blazei in two compost formulations made up with tyfton (Cynodom dactylon) and oat (Avena sativa) was evaluated. The experimental design was a totally randomized 2 x 2 x 3 factorial design with 6 repetitions. The experimental unit consisted of 12-12.5 kg of wet compost. During the spawning, 150 g of Trichoderma sp. and C. olivacearum were added to the compost. The experiment was carried out in a climatized room, with humidity between 75-90% and temperature of 28º C. The productivity averages of the ABL 99/30 isolate of A. blazei were higher than those of ABL 04/49 and Trichoderma sp. and C. olivacearum negatively influenced the production of A. blazei. The different composts (based on tyfton and oat straw) did not influence the production of basidiomata.

Gamma irradiation can control the number of psychotrophic bacteria in Agaricus bisporus during storage

We evaluated the effect of gamma irradiation doses (0, 125, 250, and 500 Gy) in control of psychrotrophic bacteria in different strains of Agaricus bisporus (ABI-07/06, ABI-05/03, and PB-1) during storage, cultivated in composts based on oat straw (Avena sativa) and Brachiaria spp. The experimental design was completely randomized in a factorial scheme 4  2  3 (irradiation doses  composts  strains), with 24 treatments, each consisting of 2 replicates, totaling 48 experimental units (samples of mushrooms). The mushrooms collected from all culture conditions were packaged in plastic polypropylene with 200 g each and subjected to Cobalt-60 irradiator, type Gammacell 220, and dose rate 0.740 kGy h–1 , according to the treatments. Subsequently, the control (nonirradiated) and other treatments were maintained at 4 ± 1°C and 90% relative humidity (RH) in a climatic chamber to perform the microbiological analysis of mushrooms on the 1st and 14th day of storage. According to the results, it was found that the highest mean colony psychotrophic count, after 14 days of storage, was observed in strain ABI-07/06 1.30 × 108 g -1 most probable number (MPN) in nonirradiated mushrooms, coming from Brachiaria grass-based compost, and this same strain under the same storage conditions, coming from the same type of compost that underwent a dose of 500 Gy, obtained a significant reduction in mean colonies of psychrotrophic bacteria (2.25 × 104 g –1 MPN). Thus, the irradiation doses tested favored reducing the number of colonies of psychrotrophic bacteria, regardless of the type of compound and strain of A. bisporus.

Desenvolvimento micelial de Lentinula edodes em meios de cultivo à base de diferentes substratos orgânicos

The radial mycelial growth of Lentinula edodes (Berk.) Pegler, strain LE-96/13, was studied in culture media prepared with organic residues extract, by using substrates prepared with pineapple (Ananas comosus (L.) Merril) crown, Astrocaryum aculeatum Meyer peel, Theobroma grandiflorum Schum shell, Musa sp. (genomic group AAB, subgroup Pacovan) peel, and Musa sp. (genomic group AAB, subgroup Prata) peel, with three supplementation levels with wheat bran (0, 10 and 20%), and incubated at 25ºC. The experimental design was totally randomized, in a 5×3 factorial scheme, adding up 15 treatments with 4 repetitions, and each repetition corresponding to a Petri dish. The diameter of the colony was evaluated daily during nine days of incubation. After that period, it was verified that the highest mycelial growth averages of strain LE-96/13 of L. edodes were found in culture media prepared with T. grandiflorum Schum shell (whose supplementation with wheat bran was favorable for Mushroom development) and A. aculeatum Meyer peel (whose supplementation did not favor the mycelial growth of L. edodes in relation to the medium not supplemented).

Yield of different white button strains in sugar cane by product-based composts

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Productivity, Leaf Nutrient Content and Soil Carbon Stocked in Agroforestry and Traditional Management of Maize (Zea mays L.)

The aim of the work was to evaluate the productivity, leaf nutrient content and soil nutrient concentration in maize (Zea mays L.) grown in sequence with black oats (Avena strigosa Schreb.) under Leucaena diversifolia alley cropping agroforestry system (AFS) and traditional management system/sole crop (without trees-TS), after two years of cultivation following a randomized block design. The experiment was carried out in the Brazilian Association of Biodynamic Agriculture, in Botucatu—S?o Paulo, Brazil. Treatments were: control (C), chemical fertilizer application (F), biomass of L. diversifolia alley cropping application (B), biomass of L. diversifolia alley cropping + chemical fertilizer application (B + F). In the second year of management it was observed that black oat yield was higher in treatments B + F and F with significant difference in relation to the others treatments in both systems, followed by treatment B. Between systems, only treatment B showed significant difference, with higher yield value corresponding to AFS, reflecting the efficiency of AFS to promote soil fertility. Maize production presented the second year of cultivation an increasing trend in all treatments in both production systems. This result may be due to the cumulative effect of mineralization and maize straw and oats, along the experiment. How productivity was higher in the AFS system, could also be occurring effect of biological nitrogen fixation, water retention and reduction of extreme microclimate through the rows of L. diversifolia. Comparing the AFS and TS, it was observed that the concentration of N in leaf tissue was higher in the AFS treatments, probably due to nitrogen fixation performed through the rows of L. diversifolia, that is a nitrogen fixing tree species. After two years, carbon stocked in soil show higher values in the treatments biomass + fertilizer and biomass application, in both systems, AFS and TS.

Doses e épocas de aplicação de nitrogênio: Influência na relação C/N da palhada no desenvolvimento e produtividade do milho em sistema plantio direto

The objective of this research work was to evaluate the effects of doses and time of application of N on the C/N ratio of the straw cover and on the growth and productivity of maize plants growing in a no tillage system. The experiment was carried out at the Experimental Farm of the College of Agriculture of the São Paulo State University (UNESP) on its campus of Botucatu, state of São Paulo, Brazil. The treatments were distributed in the field according to a randomized complete block design in a split plot arrangement. The treatments consisted of four doses of N (0, 20, 40, and 60 kg ha-1 ) applied to oat crop and N doses (60, 80, 100, and 120 kg ha-1 ) sidedressed to corn. The development and productivity of the maize crop in a no-tillage system were found to be dependent of the C/N ratio and the straw cover. The response of the maize plants to the early application of N is dependent on doses and time of application.