Trial by fire

Victoria is facing its driest summer for nearly one hundred years, with conditions worse than in 1983 when the state was swept by the devastating Ash Wednesday bushfires. The fires were particularly fierce in Mount Macedon in central Victoria, and the Woodend Fire Brigade is in the front line of the struggle to avoid a repeat of Ash Wednesday. Trial by Fire follows this brigade as they do all that they can to keep their community safe from the devastation of the fires.

A low-cost intelligent gas sensing device for military applications

The field of electronic noses and gas sensing has been developing rapidly since the introduction of the silicon based sensors. There are numerous systems that can detect and indicate the level of a specific gas. We introduce here a system that is low power, small and cheap enough to be used in mobile robotic platforms while still being accurate and reliable enough for confident use. The design is based around a small circuit board mounted in a plastic case with holes to allow the sensors to protrude through the top and allow the natural flow of gas evenly across them. The main control board consists of a microcontroller PCB with surface mount components for low cost and power consumption. The firmware of the device is based on an algorithm that uses an Artificial Neural Network (ANN) which receives input from an array of gas sensors. The various sensors feeding the ANN allow the microcontroller to determine the gas type and quantity. The Testing of the device involves the training of the ANN with a number of different target gases to determine the weightings for the ANN. Accuracy and reliability of the ANN is validated through testing in a specific gas filled environment.

Bovine Muc1 is a highly polymorphic gene encoding an extensively glycosylated mucin that binds bacteria

The bovine Muc1 protein is synthesized by mammary epithelial cells and shed into milk as an integral component of the milk fat globule membrane; however, the structure and functions of this mucin, particularly in relation to lactation, are poorly defined. The objectives of this investigation were to investigate the Muc1 gene and protein structures in the context of lactation and to test the hypothesis that Muc1 has a role in innate immune defense. Polymerase chain reaction analysis of genomic DNA from 630 cattle revealed extensive polymorphism in the variable number of tandem repeats (VNTR) in the bovine Muc1 gene. Nine allelic
variants spanning 7 to 23 VNTR units, each encoding 20 AA, were identified. Three alleles, containing 11, 14, and 16 VNTR units, respectively, were predominant. In addition, a polymorphism in one of the VNTR units has the potential to introduce a unique site for N-linked glycosylation. Statistical analysis indicated weak associations between the VNTR alleles and milk protein and fat percentages in a progeny-tested population of Holstein-Friesian dairy cattle. No association with somatic cell count could be demonstrated. Bovine Muc1 was purified from milk fat globule membranes and characterized. The protein was highly glycosylated, primarily with O-linked sialylated T-antigen [Neu5Ac(α2–3)-Gal(β1–3)-GalNAcα1] and, to a lesser extent, with N-linked oligosaccharides, which together accounted for approximately 60% of the apparent mass of Muc1. Purified bovine Muc1 directly bound fluorescently labeled Escherichia coli BioParticles (Invitrogen, Mount Waverley, Australia) and inhibited their binding to bovine mammary epithelial cells grown in vitro.

Water, weeds and autumn leaves : learning to be drier in the alpine region

Our paper explores how and what adults living and working in the Alpine region of Victoria understand and are learning about the changes to water availability, in a time when the response to water availability is subject to extensive debate and policy attention. Interviews for this study were conducted in the towns of Bright and Mount Beauty, with participants drawn from across the Alpine region. The interviews focused on what local stakeholders from the Alpine region understood about water availability in the region and how and what they had learned about living and working with climatic changes in their local area.

The findings of our study see that there was evidence of a strong understanding of the direct and indirect impact of climate change on participants' local community area. The study also sees evidence of learning through a community 'frames of reference' as outlined by Berkhout, Hertin and Dann et al.

Systemic activation of dendritic cells by toll-like receptor ligands or malaria infection impairs cross-presentation and antiviral immunity

The mechanisms responsible for the immunosuppression associated with sepsis or some chronic blood infections remain poorly understood. Here we show that infection with a malaria parasite (Plasmodium berghei) or simple systemic exposure to bacterial or viral Toll-like receptor ligands inhibited cross-priming. Reduced cross-priming was a consequence of downregulation of cross-presentation by activated dendritic cells due to systemic activation that did not otherwise globally inhibit T cell proliferation. Although activated dendritic cells retained their capacity to present viral antigens via the endogenous major histocompatibility complex class I processing pathway, antiviral responses were greatly impaired in mice exposed to Toll-like receptor ligands. This is consistent with a key function for cross-presentation in antiviral immunity and helps explain the immunosuppressive effects of systemic infection. Moreover, inhibition of cross-presentation was overcome by injection of dendritic cells bearing antigen, which provides a new strategy for generating immunity during immunosuppressive blood infections.

Climate change in the Victorian Alps : can VET be a change agent?

Of all the factors contributing to turbulent times in Australia, climate change is one that offers both challenges and opportunities for VET. In a time when the response to water availability is subject to ‘extensive debate and policy attention’, our presentation explores what adults living and working in the Alpine region of Victoria understand about the changes to water availability, and what they have learned about adapting to significant climatic changes in their local area. Interviews were conducted in the towns of Bright, Mount Beauty and Albury, with participants from across the Alpine region. Our study found evidence of a strong understanding of the direct impact of climate change on participants’ local community area, and a keen desire to learn about adaptation to change. In addition to an identified need for more information around climate change issues and projected impacts in general, participants saw practical hands-on water education strategies as an important way to educate people to help themselves. Conversations about where or how people learned to adapt to change were broad ranging, and clearly connected to the participants’ backgrounds, livelihoods or where they were situated. This raised the question of what responses VET might develop to address these identified learning needs. Major local industries
of tourism, agriculture, water harvesting and land care are all covered by national Training Packages that include industry- specific units of competence to support learning to live and work in an environmentally sustainable way. In addition, the national Employability Skills framework offers opportunities to build climate change awareness and adaptation into units of competency where they may not be explicitly incorporated. Our presentation will outline the opportunities for VET to act as a change agent in this and other Australian communities impacted by climate change.

Reinstating indigenous landscape planning curatorship : the Lake Condah restoration project

While contemporary Western planning traditions in Australia talk of the last 200 years of innovation and transposition of European and North American planning traditions upon the Australian landscape, they neglect to mention some 40-50,000 years of Indigenous landscape planning initiatives and practice. The ancestral country of the Gunditjmara people is in the Western District of Victoria focused upon the Lake Condah and Mount Eccles localities. The Gunditjmara had, and continue to have a strong social, cultural and land management and planning presence in the region, in particular linked to environmental engineering initiatives and aquaculture curatorship of eel and fish resources. Archaeological evidence confirms that some 10,000 years of pre-European contact landscape planning practice has been applied by the Gunditjmara to construct resources management infrastructure to service a regional food need as well as a community need. Within contemporary reconciliation discourses, the Gunditjmara have activity sought over the last 25 years the rehabilitation of Lake Condah, which is now coming into fruition, and the restoration of their traditional landscape planning and management responsibilities. This paper reviews the restoration of Indigenous landscape planning and management theory and practice by the Gunditjmara, pointing to significant policy and practice success as well as the need to better appreciate this culturally-attuned and ecologically-responsive approach to landscape planning borne out of generations of knowledge.

Corner inlet and Nooramunga habitat mapping project

Deakin University and the University of Tasmania were commissioned by Parks Victoria (PV) to create two updated habitat maps for areas within the Corner Inlet and Nooramunga Marine and Coastal Park and Ramsar area. The team obtained a ground-truth data set using in situ video and still photographs. This dataset was used to develop and assess predictive models of benthic marine habitat distributions incorporating data from both ALOS (Advanced Land Observation Satellite) imagery atmospherically corrected by CSIRO and LiDAR (Light Detection and Ranging) bathymetry. This report describes the results of the mapping effort as well as the methodology used to produce these habitat maps.

Overall accuracies of habitat classifications were good, returning overall accuracies >73 % and kappa values > 0.62 for both study localities. Habitats predicted with highest accuracies included Zosteraceae in Nooramunga (91 %), reef in Corner Inlet (80 %), and bare sediment (no-visible macrobiota/no-visible seagrass classes; both > 76 %). The majority of classification errors were due to the misclassification of areas of sparse seagrass as bare sediment. For the Corner Inlet study locality the no-visible macrobiota (10,698 ha), Posidonia (4,608 ha) and Zosteraceae (4,229 ha) habitat classes covered the most area. In Nooramunga no-visible seagrass (5,538 ha), Zosteraceae (4,060 ha) and wet saltmarsh (1,562 ha) habitat classes were most dominant.

In addition to the commissioned work preliminary change detection analyses were undertaken as part of this project. These analyses indicated shifts in habitat extents in both study localities since the late 1990s/2000. In particular, a post-classification analysis highlighted that there were considerable increases in seagrass habitat (primarily Zosteraceae) throughout the littoral zones and river/creek mouths of both study localities. Further, the numerous channel systems remained stable and were free of seagrass at both times. A substantial net loss of Posidonia in the Corner Inlet locality is likely but requires further investigation due to potential misclassifications between habitats in both the 1998 map (Roob et al. 1998) and the current mapping. While the unsupervised Independent Components Analysis (ICA) change detection technique indicated some changes in habitat extent and distribution, considerable areas of habitat change observed in the post-classification approach are questionable, and may reflect misclassifications rather than real change. A particular example of this is an apparent large decrease in Zosteraceae and increase in Posidonia being related to the classification of Posidonia beds as Zosteraceae in the 1998 mapping. Despite this, we believe that changes indicated by both the ICA and post-classification approaches have a high likelihood of being ‘actual’ change. A pattern of gains and losses of Zosteraceae in the region north of Stockyard channel is an example of this. Further analyses and refinements of approaches in change detection analyses such as would improve confidence in the location and extent of habitat changes over this time period.

This work has been successful in providing new baseline maps using a repeatable method meaning that any future changes in intertidal and shallow water marine habitats may be assessed in a consistent way with quantitative error assessments. In wider use, these maps should also allow improved conservation planning, advance fisheries and catchment management, and progress infrastructure planning to limit impacts on the Inlet environment.