919 resultados para Microarray Experiments


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We discuss the use of the CP asymmetry parameter (ACP) as a possible observable of CP violation in the leptonic sector. In order to do this, we study for a wide range of values of LIE the behavior of this asymmetry for the corresponding maximal value of the CP violation factor allowed by all the present experimental limits on neutrino oscillations in vacuum and the recent Super-Kamiokande atmospheric neutrino result. We work in the three neutrino flavor framework. ©1999 The American Physical Society.

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Biomass consumption and carbon release rates during the process of forest clearing by fire in five test plots are presented and discussed. The experiments were conducted at the Caiabi Farm near the town of Alta Floresta, state of Mato Grosso, Brazil, in five square plots of 1 ha each designated A, B, C, D, and E, with different locations and timing of fire. Plot A was located in the interface with a pasture, with three edges bordering on the forest, and was cut and burned in 1997. Plots B,C, D, and E were located inside the forest. Plot B was cut and burned in 1997. Plot C was inside a deforested 9-ha area, which was cut and burned in 1998. Plot D was inside a deforested 4-ha area, which was cut in 1998 and burned in 1999. Plot E was inside a deforested 4-ha area which was cut and burned in 1999. Biomass consumption was 22.7%, 19.5%, 47.5%, 61.5% and 41.8%, for A, B, C, D, and E, respectively. The effects of an extended curing period and of increasing the deforested area surrounding the plots could be clearly observed. The consumption for areas cut and burned during the same year, tended toward a value of nearly 50% when presented as a function of the total area burned. The aboveground biomass of the test site and the amount of carbon before the fire were 496 Mg ha-1 and 138 Mg ha-1, respectively. Considering that the biomass that remains unburned keeps about the same average carbon content of fresh biomass, which is supported by the fact that the unburned material consists mainly of large logs and considering the value of 50% for consumption, the amount of carbon released to the atmosphere as gases was 69 Mg ha-1. The amounts of CO2 and CO released to the atmosphere by the burning process were then estimated as 228 Mg ha-1 and 15.9 Mg ha-1, respectively. Observations on fire propagation and general features of the slash burnings in the test areas complete the paper. Copyright 2001 by the American Geophysical Union.

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The objective of this article is to apply the Design of experiments technique along with the Discrete Events Simulation technique in an automotive process. The benefits of the design of experiments in simulation include the possibility to improve the performance in the simulation process, avoiding trial and error to seek solutions. The methodology of the conjoint use of Design of Experiments and Computer Simulation is presented to assess the effects of the variables and its interactions involved in the process. In this paper, the efficacy of the use of process mapping and design of experiments on the phases of conception and analysis are confirmed. © 2007 IEEE.

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The tourism policies pursued by the Brazilian government since the 1990s have not produced the benefits that were expected from mass tourism. The example of two very successful cases of community-based tourism, stressing paths rooted in a development model that is fair and environmentally responsible, shows that tourism development can improve the quality of life in communities that receive an influx of tourists provided that the local community is taken into account and the planning and implementation of such development focus on creating opportunities and benefits for its members. © 2008 Latin American Perpectives.

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The objective of this article is to apply the Design of Experiments technique along with the Discrete Events Simulation technique in an automotive process. The benefits of the design of experiments in simulation include the possibility to improve the performance in the simulation process, avoiding trial and error to seek solutions. The methodology of the conjoint use of Design of Experiments and Computer Simulation is presented to assess the effects of the variables and its interactions involved in the process. In this paper, the efficacy of the use of process mapping and design of experiments on the phases of conception and analysis are confirmed.

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Background. From shotgun libraries used for the genomic sequencing of the phytopathogenic bacterium Xanthomonas axonopodis pv. citri (XAC), clones that were representative of the largest possible number of coding sequences (CDSs) were selected to create a DNA microarray platform on glass slides (XACarray). The creation of the XACarray allowed for the establishment of a tool that is capable of providing data for the analysis of global genome expression in this organism. Findings. The inserts from the selected clones were amplified by PCR with the universal oligonucleotide primers M13R and M13F. The obtained products were purified and fixed in duplicate on glass slides specific for use in DNA microarrays. The number of spots on the microarray totaled 6,144 and included 768 positive controls and 624 negative controls per slide. Validation of the platform was performed through hybridization of total DNA probes from XAC labeled with different fluorophores, Cy3 and Cy5. In this validation assay, 86% of all PCR products fixed on the glass slides were confirmed to present a hybridization signal greater than twice the standard deviation of the deviation of the global median signal-to-noise ration. Conclusions. Our validation of the XACArray platform using DNA-DNA hybridization revealed that it can be used to evaluate the expression of 2,365 individual CDSs from all major functional categories, which corresponds to 52.7% of the annotated CDSs of the XAC genome. As a proof of concept, we used this platform in a previously work to verify the absence of genomic regions that could not be detected by sequencing in related strains of Xanthomonas. © 2010 Moreira et al; licensee BioMed Central Ltd.

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A body of knowledge in Software Engineering requires experiments replications. The knowledge generated by a study is registered in the so-called lab package, which, must be reviewed by an eventual research group with the intention to replicate it. However, researchers face difficulties reviewing the lab package, what leads to problems in share knowledge among research groups. Besides that, the lack of standardization is an obstacle to the integration of the knowledge from an isolated study in a common body of knowledge. In this sense, ontologies can be applied, since they can be seen as a standard that promotes the shared understanding of the experiment information structure. In this paper, we present a workflow to generate lab packages based on EXPEiiQntology, an ontology of controlled experiments domain. In addition, by means of lab packages instantiation, it is possible to evolve the ontology, in order to deal with new concepts that may appear in different lab packages. The iterative ontology evolution aims at achieve a standard that is able to accommodate different lab packages and, hence, facilitate to review and understand their content.

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We present a general model of brain function (the calcium wave model), distinguishing three processing modes in the perception-action cycle. The model provides an interpretation of the data from experiments on semantic memory conducted by the authors. © 2013 Pereira Jr, Santos and Barros.

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Phylogenetic approaches based on mitochondrial DNA variation (fragments of Cyt B and 16S ribosomal RNA) have revealed Triatoma sherlocki as the most recent species addition to the Triatoma brasiliensis species complex; a monophyletic group which includes T. brasiliensis, Triatoma melanica, and Triatoma juazeirensis. T. sherlocki is the most differentiated among all species of this complex: it is unable to fly, possesses longer legs than the other members, and has reddish tonality in some parts of its exochorion. We question whether these species are reproductively compatible because of this pronounced morphological differentiation, and therefore, we present a series of cross breeding experiments that test compatibility between T. sherlocki and other members of the T. brasiliensis complex. We extended our analyses to include crosses between T. sherlocki and Triatoma lenti, because the latter has been suggested as a possible member of this complex. T. sherlocki male. ×. T. lenti female pairs failed to produce hybrids. All other crosses of T. sherlocki and members of T. brasiliensis species complex, as well as backcrosses, produced viable offspring through the third generation. This study stresses the importance of searching for the features that may isolate members of the T. brasiliensis species complex. © 2013 Elsevier B.V.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)